Fang Dong,Xiaoshan Zhu,Wei Qian,Pu Wang,Jiangxin Wang
Ocean acidification (OA) and heavy metals are common stress factors for marine ecosystems subject to anthropogenic impacts. OA coupled with the heavy metal is likely to affect marine species. This study investigated the single and combined effects of OA (1500 ppm) and cadmium (Cd; 0.4, 1.2 mg/L) on the marine diatom Phaeodactylum tricornutum under 7 d exposure. The results clearly indicated that either OA or Cd stress (1.2 mg/L) alone inhibited the growth of P. tricornutum . However, under the combined OA-Cd stress, the growth inhibition disappeared, and the intracellular oxidative damage was mitigated. These results indicated a significantly enhanced tolerance of P. tricornutum to Cd while under OA conditions, which could be beneficial to the survival of this diatom. This study will ultimately help us understand the responses of marine organisms to multiple stressors and have broad implications for the potential ecological risks of Cd under future OA conditions.
Propsilocerus akamusi (Tokunaga) is a common species of midge in Siberia , Japan, and China and an important prey species for fish and aquatic birds. Furthermore, this species has been shown to have an extraordinary capacity to resist cadmium (Cd) toxicity. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) coupled liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to analyze relative changes in the P. akamusi hemolymph proteome following exposure to a sublethal concentration of Cd 2+ . The results showed that Cd 2+ stress affects energy metabolism in P. akamusi . After examining the differentially expressed proteins (DEPs), only one up-regulated protein associated with metabolism, α-enolase (Eno1) was identified and further isolated and characterized. Sequence alignments showed that the deduced P. akamusi Eno1 amino acid sequence is highly conserved, with similarities of 77–95% noted when compared to other Dipteran Eno1 sequences. Furthermore, prolonged Cd 2+ exposure impacted Eno1 transcription, protein expression and enzyme activity levels. These results suggest that Eno1 may play a role in the response to Cd 2+ stress in P. akamusi .
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