Nature Communications
·
2022
·Vol. 13
·期 1
·1-14
·
IF 17.694
Zhou Liang,Lei Qi,Guo Jimin,Gao Yuanyuan,Shi Jianjun,Yu Hong,Yin Wenxiang,Cao Jiangfan,Xiao Botao,Andreo Jacopo,Ettlinger Romy,Jeffrey Brinker C.,Wuttke Stefan,Zhu Wei
Deoxyribonucleic acid (DNA) is the blueprint of life, and cost-effective methods for its long-term storage could have many potential benefits to society. Here we present the method of in situ cryosilicification of whole blood cells, which allows long-term preservation of DNA. Importantly, our straightforward approach is inexpensive, reliable, and yields cryosilicified samples that fulfill the essential criteria for safe, long-term DNA preservation, namely robustness against external stressors, such as radical oxygen species or ultraviolet radiation, and long-term stability in humid conditions at elevated temperatures. Our approach could enable the room temperature storage of genomic information in book-size format for more than one thousand years (thermally equivalent), costing only 0.5 $/person. Additionally, our demonstration of 3D-printed DNA banking artefacts, could potentially allow ‘artificial fossilization’.
研究领域:
生物技术
·
分子生物学
·
基因组学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Yunqing Tian,Yiming Ma,Yuxiang Kang,Shu Tian,Qingsi Li,Lei Zhang,Jing Yang
Hypothermic preservation (HP) is highly desired for the maintenance of the viability of living cell specimens, e.g. rare cells in whole-blood samples or therapeutic cells, in an unfrozen state. However, the extension of the viable preservation time is a challenge because of the multiple injuries suffered by hypothermically preserved cells. Here, based on a dynamic bond crosslinked zwitterionic hydrogel, we established a sensing preservation system that could monitor the levels of reactive oxygen species (ROS) via real-time electronic signals and intelligent control of antioxidant addition, to completely prevent an excess of ROS in the whole-cell specimen. Furthermore, the hydrogel-based system can counter the extracellular-matrix-loss-induced anoikis of living cells. Based on the design aimed at affording protection against two primary HP injuries (i.e. ROS overproduction and anoikis) to cells, this system extended the preservation time of cell specimens under refrigerated conditions to 24 days. After preservation, the use of a mild cell retrieval process guaranteed the activity of the preserved living cells. This work not only possesses the potential to facilitate intelligent cell-based clinical applications, but also paves the way for the preparation of living materials that can host programmed cells with long-term survival. Statement of significance An intelligent system based on a zwitterionic sensing hydrogel is established, which can afford ultra-long hypothermic cell-preservation times of up to 24 days. The system enables the real-time monitoring of ROS overproduction and intelligent antioxidant addition, because of the merging of the smart hydrogel with a computer intelligent detection and control system. Furthermore, the automatic addition of an antioxidant according to the ROS-signal changes produced by the ZBA hydrogel effectively prevented HP lesions, including ROS over-production and ECM loss, in the preserved living cells. Subsequently, the system could also be gently dissociated, to retrieve the preserved cells. This work provides a solution for the real-time monitoring and long-term HP of living specimens, which holds the promise of benefiting cell-based medicine and the development of genetically programmed cell-based living materials.
研究领域:
生物技术
·
细胞生物学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wenze Wang,Yiya Ping,Liang Zhang,Jianxing Feng,Wentao Zhang,Jing Sun,Jianlong Wang
To address the microbial spoilage of perishable products, this study developed an advanced multi-modal antibacterial packaging solution that combines bacterial capture and visible light-driven photothermal synergistic photodynamic antibacterial effects to effectively control microbial growth and prolong preservation. By innovatively combining curcumin-loaded porous hollow carbon spheres with a chitosan matrix, a composite film has been created that demonstrates exceptional visible light utilization performance through the integration of the following innovative characteristics: (i) enhanced electron dynamics that reduce the recombination of photo-generated electrons and holes; (ii) broadened absorption and reflective properties of PHC to maximize visible light utilization; (iii) improved carrier functionality that mitigates the tendency of curcumin to aggregate in water; and (iv) the excellent bacterial capture efficiency of chitosan for the short-range effectiveness of reactive oxygen species. The system achieved a high sterilization rate (99.9 %) after a short treatment with visible light (10 min) and effectively extended the shelf life of kumquats to 27 days. Furthermore, the low hemolysis rate (≤2.0 %) and high 3T3 cell viability (≥ 86 %) demonstrated the biosafety, laying the foundation for the practical application. Above all, the composite film holds great promise as a microbial control barrier, capable of efficiently utilizing visible light for antibacterial preservation of perishable products.
研究领域:
食品科学
·
材料科学
·
生物技术
·
微生物学
·
光动力疗法
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）