Results in Chemistry
·
2026
·
IF 5.8
Huanhuan Zhu,Yong Xu,Jiafen Huang,Yiheng Zhang,Manman Sun,Zhiming Wang,Yurong Wang
In this work, a sensitive glassy carbon electrode modified with palladium-doped electrochemically reduced graphene oxide (Pd@ErGO/GCE) was developed for the selective and simultaneous determination of tetrandrine (TET) and fangchinoline (FAN). The electrochemical behavior was investigated by cyclic voltammetry (CV) and a multimodal voltammetric platform was established: (1) differential pulse voltammetric (DPV) quantification of TET (LOD = 55 nM), (2) linear sweep voltametric (LSV) determination of total content (LOD = 45 nM), and (3) simultaneous determination of both analytes via DPV. The methods have great reproducibility and repeatability, great interference tolerance, and satisfactory accuracy in serum (recovery: 95.7% ~ 104%) and urine (97.4% ~ 103%). Comprehensive validation against HPLC-UV ( t = 0.32) confirmed method accuracy for pharmaceutical quality control in Radix Stephaniae tetrandrae. This methodology offers a reliable framework for alkaloid monitoring in herbal and biological matrices.
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高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Yujiao Tu,Ze Liu,Lei Jiang,Yingying Xiang,Fei Song,Lifen Meng,Xiuling Ji,Lin Yuan
New coccine is one of the most used red colorants in foods. Due to its cytotoxicity, quantitative determination is very necessary. Herein, the ratiometric fluorescent and UV absorption dual-mode nanosensor , derived from fangchinoline and o-phenylenediamine, was successfully prepared by hydrothermal way. The prepared carbon dots with three colors were purified with dialysis, thin layer chromatography and chromatographic column. Highly green fluorescence of Fan,N-CDs was quenched by new coccine with inner filter effect. The dependence of the ratiometric fluorescent response on new coccine concentrations was found linear over the range of 0–12.5 μM and 20–55 μM with LOD of 30.12 nM and 54.85 nm, respectively. The linearity of UV absorption ranged from 0 to 45 μM with of LOD 1.98 nM. In addition, the nanosensor was used to determine new coccine in energy drinks, carbonated beverage, gum and ketchup. It provides a new insight on the detection of new coccine by the dual-mode nanosensor.
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ONCOLOGY REPORTS
·
2020
·Vol. 45
·期 1
·139-150
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IF 3.417
Fengqi Jiang,Shuo Ren,Yaodong Chen,Ange Zhang,Yuekun Zhu,Zhenan Zhang,Zizhuo Li,Daxun Piao
Fangchinoline (FAN), an alkaloid extracted from Stephania tetrandra, has a variety of biological and pharmacological activities, but evidence of its effects on colon adenocarcinoma (COAD) is limited. Therefore, the present study aimed to elucidate the molecular mechanisms by which FAN affects COAD. The cytotoxicity, viability and proliferation of DLD‑1 and LoVo cells were assessed in the presence of FAN using MTT and colony formation assays. The effects of FAN on apoptosis and the cell cycle in COAD cells were analysed by flow cytometry, and the migration and invasion of these cells were assessed by wound healing and Transwell experiments. Furthermore, a network pharmacological analysis was conducted to investigate the target of FAN and the results were confirmed by western blotting. In addition, a xenograft model was established in nude mice, and ultrasound imaging was used to assess the preclinical therapeutic effects of FAN in vivo. To the best of our knowledge, the results of this study provided the first evidence that FAN inhibited cellular proliferation, stemness, migration, invasion, angiogenesis and epithelial‑mesenchymal transition (EMT), and induced apoptosis and G1‑phase cell cycle arrest. Network pharmacological analysis further confirmed that FAN prevented EMT through the epidermal growth factor receptor (EGFR)‑phosphoinositide 3‑kinase (PI3K)/AKT signalling pathway. Finally, FAN significantly repressed tumour growth and promoted apoptosis in xenografts. Thus, targeting EGFR with FAN may offer a novel therapeutic approach for COAD.
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PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2014
·
IF 0.638
Zhisheng Xie,Xinjun Xu,Chunyan Xie,Zhikun Liang,Mei Yang,Jieyun Huang,Depo Yang
Tetrandrine and fangchinoline were successfully separated by reversed-phase flash chromatography on a manually-packed C18 column from Radix Stephania tetrandra. After recrystallization by acetone, 13 mg of fangchinoline and 21 mg of tetrandrine were obtained from 100 mg Stephania tetrandra extract, with their relative content 98.78% and 98.19%, respectively. Their structures were characterized by UV, IR, MS, and NMR. The established method was simple, fast, and reproducible, which can be applied to the preparation of reference substances of tetrandrine and fangchinoline.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2011
·
IF 2.733
Lihong Liu,Shenglan Li,Zilin Chen
A simple and selective method was developed for the simultaneous determination of tetrandrine and fangchinoline in herbal medicine by HPLC with electrochemical detection (ECD) on a bare glassy carbon electrode. The HPLC separation and ECD conditions have been optimized. The separation was carried out on a WondaSil ® C18-WR column (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile–ammonium acetate buffer (pH 6.5; 40 mM) (32:68, v/v) using an isocratic elution at the flow rate of 0.5 mL/min. The electrochemical detection potential was set at +0.9 V. The obtained LODs for tetrandrine and fangchinoline were 0.26 and 0.27 μmol/L, respectively. The method was successfully applied to the analysis of tetrandrine and fangchinoline contents in Stephania tetrandra S. Moore. It has been demonstrated that the LC-ECD method is an excellent technique for analysis of the herbal medicine. The mean recoveries were in the range of 95–105%, while the precision expressed as repetition of peak area was lower than 2.7%.
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PubMed
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