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Foods · 2021 ·Vol. 11 ·期 1 ·92 · IF 4.35

Antibacterial Mechanism of 3-Carene against the Meat Spoilage Bacterium Pseudomonas lundensis and Its Application in Pork during Refrigerated Storage

Zhiling Tang,Haiming Chen,Ming Zhang,Zhuye Fan,Qiuping Zhong,Weijun Chen,Yong-Huan Yun,Wenxue Chen

Pseudomonas lundensisis the main bacterium responsible for meat spoilage and its control is of great significance. 3-Carene, a natural monoterpene, has been proved to possess antimicrobial activities. This study aimed to investigate the antibacterial activity and mechanism of 3-carene against the meat spoilage bacteriumP. lundensis, and explore its application on pork. After 3-carene treatment, cellular structural changes were observed. Cell walls and membranes were destroyed, resulting in the leakage of alkaline phosphatase and cellular contents. The decreased activity of Ca2+-Mg2+-ATPase and Na+-K+-ATPase showed the imbalance of intracellular ions. Subsequently, adenosine triphosphate (ATP) content and oxidative respiratory metabolism characteristics indicated that 3-carene inhibited the metabolism of the tricarboxylic acid cycle inP. lundensis. The results of binding 3-carene with the vital proteins (MurA, OmpW, and AtpD) related to the formation of the cell wall, the composition of the cell membrane, and the synthesis of ATP further suggested that 3-carene possibly affected the normal function of those proteins. In addition, the growth ofP. lundensisand increase in pH were inhibited in pork during the 5 days of cold storage after the samples were pre-treated with 3-carene. These results show the anti-P. lundensisactivity and mechanism of 3-carene, and its potential use in meat preservation under refrigerated conditions.Keywords:Pseudomonas lundensis;3-carene;preservatives;membrane damage

CAS / MDL: 6104-58-1
研究领域: 食品科学 · 微生物学 · 药理学
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY · 2015 · IF 3.082

Effects of preservatives on Alicyclobacillus acidoterrestris growth and guaiacol production

Rui Cai,Yahong Yuan,Zhouli Wang,Chunfeng Guo,Bin Liu,Chunqing Pan,Laping Liu,Tianli Yue

Alicyclobacillus acidoterrestris can survive the pasteurization process, multiply in pasteurized juices and produce guaiacol which causes medicinal or antiseptic off-flavors. Chemical preservatives have the potential to suppress outgrowth of surviving populations during subsequent storage of fruit juices. In the present study, the individual effects of potassium sorbate , sodium benzoate , potassium metabisulfite , dehydroacetic acid , ethyl 4-hydroxybenzoate, cinnamic acid and ε-polylysine on A. acidoterrestris growth and guaiacol production were firstly evaluated in a laboratory medium. Of the seven preservatives investigated, only dehydroacetic acid, cinnamic acid and ε-polylysine were effective both in controlling growth and guaiacol formation by A. acidoterrestris . Then, these three antimicrobials were applied to apple juice. Through the addition of 270   mg/L dehydroacetic acid, 108   mg/L cinnamic acid or 100   mg/L ε-polylysine, the A. acidoterrestris counts were reduced by 3.43, 3.17 and 4.78   log   colony   forming   unit   (CFU)/mL, respectively, and no guaiacol was detected after 14   days of storage. Sensory evaluation revealed that the addition of these three preservatives did not affect the organoleptic properties of the apple juice. Results obtained in this paper could be very useful for a better control of A. acidoterrestris -related spoilage in the fruit juice/beverage industry.

研究领域: 食品科学 · 微生物学
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INTERNATIONAL JOURNAL OF COSMETIC SCIENCE · 2015 ·Vol. 37 ·期 5 ·474-478 · IF 1.377

Investigation on formaldehyde release from preservatives in cosmetics

C. Lv,J. Hou,W. Xie,H. Cheng

Synopsis en This link goes to a English section fr This link goes to a French section Objective To understand formaldehyde residue in cosmetics, an investigation on formaldehyde release from eight preservatives (methenamine – MA, paraformaldehyde – PF, poly(p-toluenesulfonamide-co-formaldehyde) -PTSAF, quaternium-15 – QU, imidazolidinyl urea – IU, diazolidinyl urea – DU, dimethyloldimethyl hydantoin – DMDM and bronopol – BP) under various conditions was performed. Methods The concentration of released formaldehyde was determined by high-performance liquid chromatography with photodiode array detection after derivatization with 2,4-dinitrophenylhydrazine. Results The amounts of formaldehyde release were in the order of PF > DU > DMDM ≈ QU ≈ IU > MA > BP > PTSAF. The releasing amounts of formaldehyde were the highest in the presence of aqueous matrices for the releasers except QU and IU, and the releasing effect was also relative to pH. More formaldehyde was released with longer storage time and higher temperature. Furthermore, all preservatives in cosmetic matrices released fewer amounts of formaldehyde than in pure aqueous or organic matrices, and the formaldehyde-releasing amounts were also cosmetic specific. Conclusion Formaldehyde release was dependent on the matrix, pH, time and mainly temperature, and the releasing effect was also cosmetic specific. Résumé en This link goes to a English section fr This link goes to a French section Objectif Pour comprendre les résidus de formaldéhyde dans les produits cosmétiques, une étude du dégagement de formaldéhyde de huit conservateurs (méthénamine -MA, paraformaldéhyde -PF, poly (p-toluènesulfonamide-co-formaldéhyde) -PTSAF, quaternium-15 -QU, imidazolidinylurée -IU, diazolidinylurée -DU, dimethyloldimethyl -DMDM hydantoïne et bronopol -BP) dans diverses conditions a été réalisée. Methodes La concentration de formaldéhyde libéré a été déterminée par Chromatographie liquide à haute performance avec détection à réseau de photodiodes après dérivatisation avec du 2,4-dinitrophénylhydrazine. Résultats Les taux de dégagement de formaldéhyde étaient dans l'ordre décroissant PF> DU> DMDM ≈ UI> MA ≈ QU> BP> PTSAF. Les taux de libération de formaldéhyde étaient les plus élevés en présence de matrices aqueuses pour les libérateurs sauf QU et IU et l'effet de libération était également variables selon le pH. Plus de formaldéhyde a été libéré plus la durée du temps de stockage et la température étaient élevées. En outre, tous les conservateurs dans les matrices cosmétiques libéraient moins de quantités de formaldéhyde que dans les matrices aqueuses ou organiques purs et les taux de libération de formaldéhyde étaient également spécifique pour chaque cosmétique. Conclusion Le dégagement de formaldéhyde était dépendant de la matrice, du pH, du temps de stockage et surtout de la température et l'effet de libération était aussi spécifique de la formulation cosmétique

研究领域: 化学 · 毒理学
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Foods · 2025 ·Vol. 14 ·期 13 ·2370 · IF 5.1

Saponins from Solanum nigrum L. Fruit: Extraction Optimization, Structural Characterization, and Dual-Functional Efficacy

Shuyuan Chen,Weiyun Guo,Tonghe Zhang,Jianfang Chen,Li Huang,Jihong Huang,Ruqiang Huang

Solanum nigrumL., a widely consumed Asian medicinal edible plant, is a promising source of bioactive saponins for functional food applications. This study optimized the extraction of saponins fromS. nigrumfruits (8.59% total saponin yield), followed by isolation via column chromatography and structural elucidation using spectroscopic analyses (IR, NMR, and MS). Concurrently, the antioxidant properties and antibacterial activity of the purified substances were detected and analyzed. The three saponins (SNL1, SNL2, SNL3) were identified as γ2-Solamargine , Diosgenin, and β-Solanine. Then-butanol -purified fraction demonstrated a remarkable capacity to scavenge DPPH, hydroxyl, and ABTS radicals (DPPH IC50 = 0.0096 mg/mL; hydroxyl radical IC50 = 0.8 mg/mL; ABTS IC50 = 0.061 μg/mL), indicating the inhibition of a multi-pathway oxidative chain reaction. Concurrently, the saponins exhibited selective antimicrobial efficacy against key foodborne pathogens, particularly Escherichia coli. To the best of our knowledge, this work provides the first empirical evidence ofS. nigrumfruit saponins as dual-functional natural preservatives, synergistically suppressing lipid oxidation and microbial growth. These findings highlight their potential as safer, multi-mechanistic alternatives to synthetic additives, aligning with clean-label food industry demands.

CAS / MDL: 50-81-7
研究领域: 食品科学 · 植物化学 · 微生物学 · 药用植物学
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European Journal of Wood and Wood Products · 2014 ·Vol. 73 ·期 1 ·97-102 · IF 1.105

Toxicity of sodium fluoride to subterranean termites and leachability as a wood preservative

Pan Chengyuan,Ruan Guanhua,Chen Haihong,Zhang Dayu

Wood preservation is one of the most effective methods to prevent damage caused by termites. Sodium fluoride (NaF) is a well-known fungicide for preventing decay in timber due to its excellent diffusion in wood. In this study, the toxicity of NaF to the termites Reticulitermes flaviceps and its potential leachability as wood preservative were assessed. While termites were in contact with NaF-treated filter paper in a Petri dish for nine days, termites were found completely dead while the papers were treated by NaF at concentration of 0.5 % (wt/wt) or above. Wood blocks of Pinus massoniana were treated with five different concentrations of NaF (0.1, 0.25, 0.5, 1 and 2 %) respectively, and then exposed to a non-soil-contact field leaching experiment. After 4 weeks, retention levels of NaF in the wood blocks dropped from 0.075–0.834 % to 0.057–0.284 %. A choice test, in which NaF-treated and untreated wood blocks were exposed to the termites R. flaviceps was conducted. The results showed NaF, even at retention of 0.057 % (wt/wt), could significantly inhibit termite feeding in the treated blocks. The results suggest that NaF is a suitable wood preservative with anti-feedant activity against termites.

CAS / MDL: 7681-49-4
研究领域: 昆虫学 · 林学
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SURFACE & COATINGS TECHNOLOGY · 2024 · IF 5.3

ZIF-8 based bifunctional coatings with anticorrosive and antibacterial properties: A new design strategy for more efficiency

Wenquan Kong,Maria Serdechnova,Valeryia Kasneryk,Dahai Gao,Hao Wang,Xinyuan Xie,Carsten Blawert,Mikhail L. Zheludkevich,You Zhang

An efficient and simple in-situ/mechanical two-step strategy has been discovered for the fabrication of nanocontainers based on zeolite imidazole framework-8 (ZIF-8) with corrosion inhibitors (benzotriazole, BTA). The nanocontainers were applied in anticorrosive coatings with anticorrosion and antibacterial bifunctions for aluminum alloys AA2024. The effects of ZIF-8 modified with the corrosion inhibitor via different methods (in-situ, mechanical, and two-step strategies) on its morphology, composition, structure, loading/release behavior were studied and discussed. Results showed that the amount of loaded BTA was positively correlated with the crystal size of the final ZIF-8, impacting sustained release. Compared with the conventional primary load (in-situ and mechanical loading way), the two-step loading method (in-situ followed by mechanical treatment) achieved a higher BTA loading without evident ZIF-8 morphology and structure changes. The two-step modified (2-BTA@ZIF-8) powders exhibited good corrosion inhibition and remarkable antibacterial properties against Escherichia coli and Staphylococcus aureus . Additionally, the 1-BTA@ZIF-8 (in-situ strategy), 1-BTA/ZIF-8 (mechanical strategy) and 2-BTA@ZIF-8 composites were dispersed in acrylic resin to prepare anticorrosive and antibacterial bifunctional coatings. Results showed that the 2-BTA@ZIF-8 incorporated acrylic coatings exhibited superior anticorrosion performance and antimicrobial activity compared to the unmodified coating due to the controlled release of BTA. Thus, elaborated strategy represents a feasible and effective way to construct high-performance anticorrosive and antimicrobial coatings with metal-organic framework materials.

研究领域: 材料科学 · 表面工程 · 防腐技术 · 微生物学
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Foods · 2023 ·Vol. 12 ·期 12 ·2275 · IF 5.2

Potential of Syzygnium polyanthum as Natural Food Preservative: A Review

Nur Julizan,Safri Ishmayana,Achmad Zainuddin,Pham Van Hung,Dikdik Kurnia

Food preservation is one of the strategies taken to maintain the level of public health. Oxidation activity and microbial contamination are the primary causes of food spoilage. For health reasons, people prefer natural preservatives over synthetic ones.Syzygnium polyanthumis widely spread throughout Asia and is utilized as a spice by the community.S. polyanthumhas been found to be rich in phenols, hydroquinones, tannins, and flavonoids, which are potential antioxidants and antimicrobial agents. Consequently,S. polyanthumpresents a tremendous opportunity as a natural preservative. This paper reviews recent articles aboutS. polyanthumdating back to the year 2000. This review summarizes the findings of natural compounds presented inS. polyanthumand their functional properties as antioxidants, antimicrobial agents, and natural preservatives in various types of food.Keywords:Syzygnium polyanthum;antioxidant;antimicrobials;natural preservative;food preservation

研究领域: 植物学 · 食品科学 · 药理学
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CHROMATOGRAPHIA · 2024 · IF 1.2

Simultaneous Analytical Method for Various Paraben and Alcohol Preservatives in Supposedly Natural and Organic Cosmetics by Off-line SPE–UHPLC–DAD and LC–MS/MS

Han Kyoung-Moon,Woo In Suk,Park Chae Gyeong,Kim You Kyung,Yang Yoon Ji,Park Bo Reum,Choi Jang Duck,Son Kyung Hun

Preservatives can be used in natural and organic cosmetics to prevent quality deterioration during distribution and use, but the ingredients permitted are limited in the Republic of Korea. A method of simultaneous analysis was developed to monitor natural and organic cosmetics distributed online for the presence of 15 illegally used preservatives. Considering the complexities of the cosmetic matrices, an optimized sample preparation method is essential. Therefore, we developed the optimal purification method by comparing three preparation methods: QuEChERS, high-speed centrifugation, and solid-phase extraction (SPE). The SPE method, which exhibited the most favorable results in matrix reduction, was employed. To establish the SPE-ultra-high-performance liquid chromatography-diode array detection method, factors such as the mobile phase and column were optimized. The optimal separation of the 15 preservatives was observed under the following conditions: mobile phase A comprising 0.1% phosphoric acid in water with 0.5 mM sodium hexanesulfonate and a C 8 column (1.7 μm, 2.1 × 150 mm) as the stationary phase. The linear correlation coefficients were > 0.9998, and the limits of detection and quantitation ranged from 0.05 to 0.67 and 0.15 to 2.02 μg mL −1 , respectively. Using the established analytical method, illegally used preservatives were detected in products claimed to be natural and organic cosmetics sold on the market, and these findings were subsequently verified using liquid chromatography-tandem mass spectrometry. Six out of 47 samples contained phenoxyethanol and parabens. Therefore, this method will help manage the addition of illegal preservatives to natural and organic cosmetics.

CAS / MDL: 104-29-0 122-99-6
研究领域: 分析化学 · 化妆品科学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY · 2021 · IF 5.279

Efficient Preparation of Sophorolipids and Functionalization with Amino Acids to Furnish Potent Preservatives

Yujing Tang,Mingjie Jin,Tianyou Cui,Yang Hu,Xuwei Long

The development of new preservatives is an ongoing investigation in the food industry, especially those which are safe and environmentally friendly. In this study, biosurfactant sophorolipids (SLs) functionalized with amino acids were developed as efficient preservative agents. SLs were first isolated from fermentation broth by Candida bombicola ATCC 22214, hydrolyzed, and purified by extraction. The typical recovery is around 70%, while the extracted material consists of over 90% deacetylated acidic SLs (SL-COOH). Four types of SL derivatives were then synthesized via dicyclohexylcarbodiimide amidation reactions from prepared SL-COOH. Among the derivatives produced, the arginine SL conjugates (SL-d-Arg) displayed the highest activity against Gram-positive bacteria and fungi and even inhibited the cell growth of Gram-negative bacteria and mildew. Furthermore, the arginine conjugates performed the broadest antimicrobial activity among the derivatives evaluated. The sterilization dosage of the arginine conjugates against the food-spoilage pathogen Bacillus spp. was 63–125 mg/L, in contrast to 250 mg/L for the enterotoxin producer Staphylococcus aureus and 500 mg/L for fungi. More importantly, SL-d-Arg displayed excellent biocompatibility, with a therapeutic index of over 7.94. SL-d-Arg has excellent potential as an alternative to traditional chemical preservatives.

研究领域: 食品科学 · 微生物学 · 生物化学
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JOURNAL OF SEPARATION SCIENCE · 2014 ·Vol. 37 ·期 16 ·2248-2252 · IF 2.594

Determination of preservatives in soft drinks by capillary electrophoresis with ionic liquids as the electrolyte additives

Bingbing Sun,Li Qi,Minglin Wang

A capillary electrophoresis method for separating preservatives with various ionic liquids as the electrolyte additives has been developed. The performances for separation of the preservatives using five ionic liquids with different anions and different substituted group numbers on imidazole ring were studied. After investigating the influence of the key parameters on the separation (the concentration of ionic liquids, pH, and the concentration of borax), it has been found that the separation efficiency could be improved obviously using the ionic liquids as the electrolyte additives and tested preservatives were baseline separated. The proposed capillary electrophoresis method exhibited favorable quantitative analysis property of the preservatives with good linearity ( r 2 = 0.998), repeatability (relative standard deviations ≤ 3.3%) and high recovery (79.4–117.5%). Furthermore, this feasible and efficient capillary electrophoresis method was applied in detecting the preservatives in soft drinks, introducing a new way for assaying the preservatives in food products.

研究领域: 分析化学 · 食品科学
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JOURNAL OF MOLECULAR LIQUIDS · 2017 · IF 3.648

An example of green copper corrosion inhibitors derived from flavor and medicine: Vanillin and isoniazid

Shi Mo,Ling Jie Li,Hong Qun Luo,Nian Bing Li

The use of some industrial materials such as medicine and flavor in the anti-corrosion field is an attractive topic. A Schiff base ( N -isonicotinamido-3-methoxy-4-hydroxybenzalaldimine, IM) based on flavor (vanillin) and medicine (isoniazid) has been synthesized and utilized as a green corrosion inhibitor for copper in NaCl media. Electrochemical techniques are employed to analyze its inhibition property. The copper surface is characterized by scanning electron microscopy, infrared spectroscopy , and energy-dispersive X-ray spectroscopy. IM acts as an anodic inhibitor and its inhibition efficiency is up to 93% at 120   mg/L and more remarkable than its corresponding reactants. With a large molecular surface area and better electron donation ability, IM has a better corrosion inhibition performance and may be adsorbed on the copper surface through NH and C N groups, according to theoretical studies.

CAS / MDL: 121-33-5 54-85-3
研究领域: 材料科学 · 电化学 · 表面科学
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Food Packaging and Shelf Life · 2024 · IF 8.5

Edible freshness-keeping film prepared by Pouteria campechiana pericarp residue and applied to the preservation of duck breast meat

Ren Liu,Kejun Li,Liangyue Fang,Wanru Luo,Shuangfei Wang,Chongxing Huang

The global waste rate of fresh meat products is approximately 20 – 30%, with the vast majority occurring in the consumer and retailer sectors. The use of antimicrobial preservative films can reduce fresh retail meat waste. In this study, we propose an environmentally friendly antimicrobial preservative film prepared using the residue of Pouteria campechiana pericarp as a substrate, sodium alginate as the film-forming agent, and nisin as the antimicrobial agent. This film was used to preserve duck breast meat. Nisin in the film slows the spoilage of duck breast meat by inhibiting and killing microorganisms, thereby extending its four-day shelf life. It is important to note that mouse-feeding experiments on the film have proven that it is safe and edible. This strategy provides ideas for the reuse of agricultural waste, and this edible antimicrobial film has great potential for the preservation of fresh meat.

研究领域: 食品科学 · 生物技术 · 环境科学
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