RSC Advances
·
2015
·Vol. 5
·期 39
·30631-30639
·
IF 3.84
Haiyan Mao,Fan Yang,Chaoxia Wang,Youjiang Wang,Donggang Yao,Yunjie Yin
Dye-bonded blocked waterborne polyurethanes were developed to synchronously realize coloring and finishing of textiles and improve colorfastness using an anthraquinone chromophore and a methyl ethyl ketoxime blocking agent. The number-average molecular weight of the dye-bonded blocked polyurethane is 2303 g mol−1 and the polydispersity is 1.06, indicating a narrow molecular weight distribution. The maximum absorption wavelengths do not shift after the monomolecular chromophores are bonded to the polyurethane. The self-colored polyurethanes provide better centrifugal stability and water solubility than the dye-mixed blocked polyurethanes. The dye-bonded blocked polyurethane offers good thermal stability below 100 °C and undertakes de-blocking at about 150 °C. The covalent bonding of the anthraquinone moiety with the polyurethane chain and temporary blocking of the terminal isocyanate groups are conducive to enhancing the tinting strength and colorfastness of the treated cotton fabrics. The self-colored blocked polyurethanes with good elasticity and toughness can improve the recovery angle of the treated cotton up to 120°, which can also endow the fabric with wrinkle resistance.
研究领域:
材料科学
·
纺织化学
·
染料化学
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DOI
高影响因子
Journal of Analysis and Testing
·
2024
·
IF 5.5
Zhang Dan,Zhou Guobao,Yang Hongyan,Wang Yi,Shen Lijun,Qiu Yuxuan,Li Lei,Guo Longhua
In this work, we proposed a ratiometric silver nanoclusters (AgNCs) fluorescent assay by designing a bifunctional-blocker-aided hybridization chain reaction (HCR). Hairpin probe 1 (HP1) containing two special DNA fragments (5′-CACCGCT-3′ and 5′-ATTTGCCTTTTGGGGACGGATA-3′) at two terminals creates a red-emitting AgNC nucleation sequence (rNS, 5′-CACCGCTATTTGCCTTTTGGGGACGGATA-3′). We found that the presence of a toehold fragment (5′-TGCCC-3′) in HP1 could silence the rNS. Upon the addition of a target nucleic acid, HCR of HP1 and hairpin probe 2 (HP2) could be initiated, resulting in the formation of long chain of DNA duplexes with multibranched rNS. As the toehold fragment in HP1 participated in generating duplexes, a strong emission of rNS-templated AgNCs was observed at 670 nm. More significantly, a bifunctional blocker was introduced not only to reduce the background red-emitting fluorescence but also to play as an internal green-emitting AgNCs nucleation sequence. On the one hand, the blocker could increase the signal-to-noise-ratio of the constructed biosensor, and on the other hand, the blocker also helped to prepare ratiometric HCR-AgNCs assay with self-calibrating ability to strengthen its reproducibility. Compared with the traditional HCR-AgNCs sensors, the developed ratiometric assay based on the bifunctional-blocker-aided HCR has higher reliability, which is important for the fabrication of biosensors in various fields for practical biosensing applications.
研究领域:
生物化学
·
分子生物学
·
纳米生物学
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产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Yimei Zhang,Shiyi Xiang,Yayi Wu,Can Yang,Dianyong Tang,Zhongzhu Chen,Zheng Huang
Photodynamic therapy (PDT) combined with immunotherapy has become a promising antitumor strategy. However, precise regulation of the activation of antitumor immunity and effective reversion of immunosuppressive tumor microenvironment (TME) remains challenging. In this paper, a novel co-delivery nanomedicine is developed to solve these issues for photodynamic amplified immunotherapy. Specifically, the glycolysis inhibitor (Lon) is coupled with PD1/PDL1 blocker (BMS-1) by thioketal linkage to form smartly responsive prodrug LTB , which could further encapsulate photosensitizer chlorine e6 (Ce6) to construct a co-delivery nanoplatform ( LTB-6 NPs ) by self-assembly. Of note, LTB-6 NPs possess favorable stability, uniform morphology and improved cellular uptake. More importantly, LTB-6 NPs are capable of inhibiting glycolysis and blocking PD1/PDL1, which could greatly improve the immunosuppressive TME to promote immune activation. LTB-6 NPs -mediated PDT not only inhibits tumor proliferation but also induces ICD response to activate immunological cascade. In vivo experiments indicate that intravenously injected LTB-6 NPs remarkably suppresses the tumor growth while leads to a minimized side effect. This research provides a multi-synergized strategy for developing effective photodynamic nanoplatforms in tumor treatment.
研究领域:
肿瘤学
·
纳米医学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Guangxu Yang,Zhenggang Gong,Lizhen Huang,Xiaolin Luo,Li Shuai,Jing Liu
Enzymatic hydrolysis of cellulose in lignocelluloses exhibits low efficiency due to low cellulose accessibility and adverse lignin effects. By using phenylsulfonic acid pretreatment to improve cellulose accessibility, we have developed several new plant proteins such as corn germ and green rapeseed proteins that extracted from inexpensive defatted meals as lignin-blockers. For pretreated lignocellulosic substrates with high cellulose accessibility, such plant proteins could effectively overcome negative lignin effects and achieve robust cellulose enzymatic conversion (88–97 %) at a low cellulase loading of 5 FPU/g glucan . To reach the same level of cellulose enzymatic conversion, adding corn germ protein could save cellulase loading by 3.6 times as compared to those without lignin-blockers. But these plant proteins showed insignificant promotion effects on substrates with low cellulose accessibility. Overall, this study coupled high cellulose-accessible substrates and efficient lignin-blockers to synergistically improve the profitability of lignocellulosic biorefinery.
研究领域:
生物化学
·
可再生资源与环境工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xiaoyu Du,Yuqin Wang,Shanyu Zhang,Pingping Fan,Shuanghong Yan,Panke Zhang,Hong-Yuan Chen,Shuo Huang
Blockers of pore-forming toxins (PFTs) limit bacterial virulence by blocking relevant channel proteins. However, screening of desired blockers from a large pool of candidate molecules is not a trivial task. Acknowledging its advantages of low cost, high throughput, and multiplicity, DiffusiOptoPhysiology (DOP), an emerging nanopore technique that visually monitors the states of individual channel proteins without using any electrodes, has shown its potential use in the screening of channel blockers. By taking different α-hemolysin (α-HL) mutants as model PFTs and different cyclodextrins as model blockers, we report direct screening of pore blockers solely by using fluorescence microscopy. Different combinations of pores and blockers were simultaneously evaluated on the same DOP chip and a single-molecule resolution is directly achieved. The entire chip is composed of low-cost and biocompatible materials, which is fully disposable after each use. Though only demonstrated with cyclodextrin derivatives and α-HL mutants, this proof of concept has also suggested its generality to investigate other pore-forming proteins.
研究领域:
生物物理学
·
纳米技术
·
分子生物学
·
毒理学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Zheng-Meng Jiang,Wen-Jin Liu,Ying Li,Jie Liu,Hui-Ying Wang,Ping Li,E-Hu Liu
In this work, for the first time, 10 isoquinoline alkaloids (IQAs) were separated and determined by supercritical fluid chromatography (SFC) utilizing eco-friendly deep eutectic solvents (DESs) as novel mobile phase additives. The effects of DES types, DES concentrations, and other chromatographic parameters on the separation of IQAs were systematically optimized. Improved resolution of alkaloids and decreased peak tailing were distinctly observed when DESs were added to the mobile phase. The supercritical CO2 and MeOH-2% H2O-0.5% FA-0.25% ChCl-Gly were used as the optimized mobile phase, and the 10 IQAs were well separated in SFC. The separation mechanism might be attributed to the fact that DESs were used as silanol blockers to compete for the adsorption sites with IQAs on the silica surface. The present study suggests that the separation of IQAs from complex samples in SFC can be effectively improved using DESs as mobile phase additives. Moreover, the proposed “green” method for analyzing IQAs focuses on reducing the use of organic solvents and improving the applicability for alkaloid analysis.
研究领域:
分析化学
·
环境科学
·
药学
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产品关联
DOI
高影响因子
International Heart Journal
·
2024
·Vol. 65
·期 6
·1095-1106
·
IF 1.2
Xiaoping Zhu,Shengnan Han,Yulu Geng,Wenzhi Ren,Fushi Quan
Stroke poses a serious risk to human health. The incidence of stroke is increasing year by year. In this study, a rat cerebral ischemiareperfusion (middle cerebral artery occlusion) model was established, and the reliability of this model was determined.The expression changes of key proteins of the BDNF TrkB pathway and synaptic plasticity markers (SYP, PSD-95, and MAP-2) were detected at different time points.The results showed that BDNF expression increased rapidly in the acute phase, and the expression of synaptic plasticity markers decreased, and blocking the BDNF-TrkB pathway with the specific blocker K252a also had a significant effect.This suggests that changes in the BDNF-TrkB signaling pathway can affect the synaptic plasticity in nerve cells of stroke patients.
CAS / MDL:
研究领域:
神经科学
·
分子生物学
·
中风研究
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Zeyu Ma,Jingyu Wang,Xing Lu,Guobao Zhou,Yi Wu,Dan Zhang,Lei Li,Longhua Guo
Heterogeneous electrochemical DNA biosensors have attracted huge attention due to their enhanced signal sensitivity, compared to homogeneous biosensors. However, the high cost of probe labeling and the reduced recognition efficiency associated with current heterogeneous electrochemical biosensors confine their potential applications. In the present work, a dual-blocker assisted and dual-label-free heterogeneous electrochemical strategy based on multi-branched hybridization chain reaction (mbHCR) and reduced graphene oxide (rGO) was fabricated for ultrasensitive detection of DNA. The target DNA could trigger the mbHCR of two DNA hairpin probes, resulting in the generation of multi-branched long chain of DNA duplexes with bidirectional arms. One direction of the multi-branched arms in the mbHCR products were then bound to the label-free capture probe on the gold electrode through multivalent hybridization with enhanced recognition efficiency. The other direction of multi-branched arms in mbHCR product could adsorb rGO via π-π stacking interactions. Two DNA blockers were ingeniously designed to block the binding of excessive H1-pAT on electrode and to prevent the adsorption of rGO by residual unbound capture probes. As a result, with the electrochemical reporter methylene blue selectively intercalated into the long chain of DNA duplex and absorbed on rGO, a remarkable electrochemical signal rise was observed. Thus, a dual-blocker aided and dual-label-free electrochemical strategy for ultrasensitive DNA detection is readily realized with the merit of cost-effective. The as-developed dual-label-free electrochemical biosensor has great potential to be employed in nucleic acid related medical diagnostics.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
分子诊断
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 16
·3652-3661
·
IF 5.227
Qinghao Cao,Chao Chen,Jun Huang,Dantong Shen,Haolin Chen,Huiqing Zhong,Zhiming Liu,Zhouyi Guo
The flexible surface-enhanced Raman scattering (SERS) platform has ceaselessly propelled the development of point-of-care testing (POCT) in diverse fields. Herein, we report a facile strategy for the SERS-chemometric analysis of four β-blockers (bisoprolol, metoprolol, acebutolol and esmolol) based on a super-sticky mussel-inspired hydrogel SERS tape. The surface morphology and mechanical properties of the hydrogel tape can be easily controlled by adjusting the compositional ratio. The optimized tape with excellent toughness and adhesiveness allows efficient collection of analytes through a simple “paste and peel off” approach, further by spraying with silver nanoparticles using a household sprayer to instantly assemble a flexible SERS substrate, the analytes can then detected by a portable Raman spectrometer. This POCT strategy enables the identification and discrimination of four similar β-blockers with high sensitivity and accuracy in combination with the statistical algorithms. The developed SERS tape is finally utilized for the recognition of β-blockers in simulated urine solution, which realizes a limit of detection of 1.0 ng mL−1, revealing a promising potential of this SERS-based POCT for the clinical detection of doping abuse.
研究领域:
材料科学
·
分析化学
·
生物医学工程
·
药物分析
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Yihao Zhu,Yajian Li,Xuwen Li,Yuan Yu,Lingpu Zhang,Hanchen Zhang,Can Chen,Dong Chen,Mingshuai Wang,Nianzeng Xing,Feiya Yang,Wahafu Wasilijiang,Xiongjun Ye
Immune checkpoint blockers (ICBs) therapy stands as the first-line treatment option for advanced renal cell carcinoma (RCC). However, its effectiveness is hindered by the immunosuppressive tumor microenvironment (TME). Sonodynamic therapy (SDT) generates tumor cell fragments that can prime the host's antitumor immunity. Nevertheless, the hypoxic microenvironment and upregulated autophagy following SDT often lead to cancer cell resistance. In response to these challenges, a hypoxia-responsive polymer (Poly(4,4′-azobisbenzenemethanol-PMDA)-mPEG 5k , P-APm) encapsulating both a HIF-2α inhibitor (belzutifan) and the ultrasonic sensitize (Chlorin e6, Ce6) is designed, to create the nanoparticle APm/Ce6/HIF. APm/Ce6/HIF combined with ultrasound (US) significantly suppresses tumor growth and activates antitumor immunity in vivo. Moreover, this treatment effectively transforms the immunosuppressive microenvironment from “immune-cold” to “immune-hot”, thereby enhancing the response to ICBs therapy. The findings indicate that APm/Ce6/HIF offers a synergistic approach combining targeted therapy with immunotherapy, providing new possibilities for treating RCC.
研究领域:
肿瘤学
·
生物医学工程
·
免疫学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Rongrong Zheng,Ni Yang,Qiuyuan Li,Zuxiao Chen,Chuyu Huang,Linping Zhao,Xin Chen,Shiying Li
The combination of therapy-induced immunogenic cell death (ICD) and immune checkpoint blockade can provide a mutually reinforced strategy to reverse the poor immunogenicity and immune escape behavior of tumors. In this work, a chimeric peptide-engineered immunostimulant (ER-PPB) is fabricated for endoplasmic reticulum (ER)-targeted photodynamic immunotherapy against metastatic tumors. Among which, the amphiphilic chimeric peptide (ER-PP) is composed of ER-targeting peptide FFKDEL, hydrophilic PEG 8 linker and photosensitizer protoporphyrin IX (PpIX), which could be assembled with a PD-1/PD-L1 blocker (BMS-1) to prepare ER-PPB. After passively targeting at tumor tissues, ER-PPB will selectively accumulate in the ER. Next, the localized PDT of ER-PPB will produce a lot of ROS to destroy the primary tumor cells, while increasing the ER stress to initiate a robust ICD cascade. Moreover, the concomitant delivery of BMS-1 can impede the immune escape of tumor cells through PD-1/PD-L1 blockade, thus synergistically activating the immune system to combat metastatic tumors. In vitro and in vivo results demonstrate the robust immune activation and metastatic tumor inhibition characteristics of ER-PPB, which may offer a promising strategy for spatiotemporally controlled metastatic tumor therapy.
研究领域:
癌症研究
·
免疫疗法
·
药学
·
分子生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Congjun Xu,Haolan Yang,Zhanghong Xiao,Tao Zhang,Zilin Guan,Jie Chen,Hualu Lai,Xiaoyu Xu,Yanjuan Huang,Zeqian Huang,Chunshun Zhao
The pentose phosphate pathway (PPP) plays a critical role by providing ribulose-5-phosphate (Ru5P) and NADPH for nucleotide synthesis and reduction energy, respectively. Accordingly, blocking the PPP process may be an effective strategy for enhancing oxidation therapy and inhibiting cell replication. Here, we designed a novel reduction-responsive PEGylated prodrug and constructed nanoparticles [email protected] to simultaneously deliver a PPP blocker, dehydroepiandrosterone (DHEA), and chemotherapeutic camptothecin (CPT) to integrate amplification of oxidation therapy and enhance cell replication inhibition. Following cellular uptake, DHEA and CPT were released from [email protected] in the presence of high glutathione (GSH) levels. As expected, DHEA-mediated reduction level decreases and CPT-induced oxidation level increases synergistically, breaking the redox balance to aggravate cancer oxidative stress. In addition, suppressing nucleotide synthesis by DHEA through the reduction of Ru5P and blocking DNA replication by CPT further motivates a synergistic inhibition effect on tumor cell proliferation . The results showed that [email protected] featuring multimodal treatment has satisfactory antitumor activity both in vitro and in vivo . This study provides a new tumor treatment strategy, which combines the amplification of oxidative stress and enhancement of inhibition of cell proliferation based on inhibition of the PPP process.
研究领域:
药理学
·
肿瘤学
·
纳米技术
·
生物化学
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）