NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 44
·期 30
·12962-12966
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IF 3.288
Jing Sun,Rui Wang,Meng Xia,Shuyun Zhu,Xian-En Zhao
As alternatives to nanozymes, some metal ions have been used in chemical/biochemical sensors owing to their facile acquisition avoiding tedious preparation and purification. Ce4+ has been reported to possess oxidase-like activity. Ascribed to its strong oxidative ability, Ce4+ can trigger the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue product oxTMB without the requirement of H2O2. When ALDS is added, the oxidation ability of Ce4+ can be reduced because of the redox reaction between Ce4+ and ALDS. The absorbance intensity ascribed to oxTMB decreases followed by the fading of the solution. On the basis of such a mechanism, a simple method is proposed for monitoring ALDS in two linear ranges of 0.1–10.0 μM and 10.0–60.0 μM. The detection limit is low to 0.05 μM. Furthermore, this method exhibits great potential for ALDS detection in real biological body fluids and pharmaceutical samples.
研究领域:
分析化学
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药物分析
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产品关联
DOI
高影响因子
ONCOLOGY RESEARCH
·
2024
·
IF 2
HUAN GAO,JIE ZHANG,TONY G. KLEIJN,ZHAOYONG WU,BING LIU,YUJIN MA,BAOYUE DING,DONGFENG YIN
Bone metastasis secondary to breast cancer negatively impacts patient quality of life and survival. The treatment of bone metastases is challenging since many anticancer drugs are not effectively delivered to the bone to exert a therapeutic effect. To improve the treatment efficacy, we developed Pluronic P123 (P123)-based polymeric micelles dually decorated with alendronate (ALN) and cancer-specific phage protein DMPGTVLP (DP-8) for targeted drug delivery to breast cancer bone metastases. Doxorubicin (DOX) was selected as the anticancer drug and was encapsulated into the hydrophobic core of the micelles with a high drug loading capacity (3.44%). The DOX-loaded polymeric micelles were spherical, 123 nm in diameter on average, and exhibited a narrow size distribution. The in vitro experiments demonstrated that a pH decrease from 7.4 to 5.0 markedly accelerated DOX release. The micelles were well internalized by cultured breast cancer cells and the cell death rate of micelle-treated breast cancer cells was increased compared to that of free DOX-treated cells. Rapid binding of the micelles to hydroxyapatite (HA) microparticles indicated their high affinity for bone. P123-ALN/DP-8@DOX inhibited tumor growth and reduced bone resorption in a 3D cancer bone metastasis model. In vivo experiments using a breast cancer bone metastasis nude model demonstrated increased accumulation of the micelles in the tumor region and considerable antitumor activity with no organ-specific histological damage and minimal systemic toxicity. In conclusion, our study provided strong evidence that these pH-sensitive dual ligand-targeted polymeric micelles may be a successful treatment strategy for breast cancer bone metastasis.
研究领域:
肿瘤学
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药剂学
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DOI
PubMed
高影响因子
RSC Advances
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2018
·Vol. 8
·期 40
·22764-22776
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IF 2.936
Lei Liu,Xiaoyu Li,Xuetao Shi,Yingjun Wang
In a minimally invasive procedure, hydrogels can be injected into an affected area for drug loading and tissue repair. Here, gelatin methacryloyl (GelMA) was modified with alendronate (ALN), and three different alendronate-functionalized GelMA (GelMA-ALN) hydrogels were prepared. The modification greatly improved the swelling ratio, protein adsorption and mineralization. The GelMA-ALN hydrogels significantly promoted the in vitro osteogenic differentiation of hFOB cells as indicated by their higher alkaline phosphatase (ALP) activity, denser mineralization and up-regulated osteogenesis-related genes at both the mRNA and protein levels. Meanwhile, the cells maintained their activity and differentiated into osteoblasts when encapsulated in the GelMA-ALN hydrogels. Alendronate-modified hydrogels have potential for use in the minimally invasive treatment of irregular bone defects.
研究领域:
生物医学工程
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组织工程
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材料科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Meng Xia,Fengjin Shi,Yinghui Xia,Jing Sun,Xian-En Zhao,Shuyun Zhu
A ratiometric fluorescence assay for alendronate (ALDS) has been designed with Ce 4+ -triggered cascade chromogenic reaction. This strategy involves three processes: (1) Ce 4+ oxidizes ascorbic acid (AA) into dehydroascorbic acid (DHAA), which then condenses with o -phenlenediamine (OPD) to generate fluorescent 3-(dihydroxyethyl)furo[3,4- b ] quinoxaline-1-one (DFQ), presenting the maximum emission at 434 nm; (2) As oxidase-mimics, Ce 4+ can oxidize OPD into fluorescent 2,3-diaminophenazine (DAP) which shows a strong emission at 568 nm; (3) ALDS inhibits the oxidation ability of Ce 4+ towards OPD, thus inhibiting the generation of DAP. Accordingly, a homogeneous ratiometric fluorescence system with dual emission comes into being and the presence of ALDS can change the fluorescence intensity ratio obviously. With F 434 / F 568 as readout, ALDS can be detected sensitively with the detection limit of 30 nM. Moreover, this ratiometric method was used to analyze ALDS in both human serum and pharmaceutical samples.
研究领域:
分析化学
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药物分析
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生物医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2013
·
IF 2.947
Baogang Xie,Aihong Liu,Xiuzhong Fang,Ying Chen,Haijun Zhong
Determination of alendronate is crucial to routine quality control of alendronate tablets. However, tedious sample treatment processes such as derivatization were generally required by chromatographic separation of alendronate as its high polarity and no chromophore in the molecular structure. Here, we describe the use of 1 H NMR for the quantification of alendronate sodium in tablets. Linearity, recovery, selectivity and sensitivity of the assay were validated to be satisfactory with quick sample preparation and acquisition. The contents of alendronate sodium in tablets from five manufacturers were determined, the results showed that all assayed tablets fell within the range of 90.0–110.0% of the label claim and the relative standard deviation was less than 6.0%. The method was also successfully employed for alendronate tablet dissolution assay in this study.
研究领域:
药学
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分析化学
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DOI
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Lei Yu,Wentao Wang,Chang Lv,Qian Chen,Peng Yang,Zhenrong Qi,Haomiao Yu,Ruiqi Cao,Wenhao Li,Yi Qin,Gaoran Ge,Peilai Liu,Lixin Zhu,Houyi Sun,Dechun Geng,Liang Zhang
Osteoporotic bone regeneration poses significant challenges due to the complexity of the condition. Osteoporosis, a degenerative disorder, results from an imbalance in bone homeostasis driven by dysregulation of osteoblast and osteoclast activity. This complicates the treatment of osteoporosis and its related bone injuries in clinical practice. Despite the development of various polymer scaffolds for bone defect repair, achieving effective regeneration in osteoporotic bones—especially when combined with osteoporosis medications—remains difficult. In this study, we designed a drug delivery system composed of mesoporous bioactive glass (MBG) and photo-crosslinked hyaluronic acid methacrylate (HAMA). This system, loaded with the osteogenesis-promoting peptide DWIVA (D5) and the osteoclastogenesis-inhibiting drug alendronate (ALN), is gelled using a light initiator and 405 nm wavelength light. The MBG@D5-Gel complex enables the controlled spatiotemporal release of these agents, markedly enhancing bone regeneration in osteoporotic conditions within ovariectomized rats by inhibiting osteoclastogenesis and bone resorption while promoting osteogenic differentiation and mineralization. This dual-action system synergistically regulates osteoblast and osteoclast activity, optimizing the pathological microenvironment of osteoporosis and facilitating the repair of osteoporotic bone defects. MBG@D5-Gel holds great potential as an effective organic-inorganic hybrid biomimetic implant material for the treatment of osteoporotic bone defects.
研究领域:
生物医学工程
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骨科
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材料科学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2021
·
IF 5.268
Pu Zhang,Wanqiang Xia,Ping Deng,Yuanhong Min,Juan Tan,Yi Wang,Wensheng Fu
Peroxidase-like activity of MoS 2 quantum dots (QDs) can be reversibly regulated by means of Fe 3+ /alendronate sodium (ALDS)-induced aggregation/disaggregation of the QDs in solution. Specifically, Fe 3+ can selectively aggregate the MoS 2 QDs and thus greatly enhance their peroxidase-like activity, while such enhancement can be inhibited in the presence of ALDS owing to the competitive coordination of ALDS with Fe 3+ . By regulating the enzyme-like activity of MoS 2 QDs, different colorimetric signal of a typical substrate of horseradish peroxidase , 3,3΄,5,5΄-tetramethylbenzidine, can be measured in the presence of H 2 O 2 . Based on this mechanism, we develop a colorimetric approach for the determination of ALDS and further applied in quality control of pharmaceutical products, utilizing either smartphone or UV–vis spectrometer as a readout. This detection method is rapid and selective, where derivatization of ALDS before detection is not needed. Such a smartphone-based colorimetric detection platform is promising to be applied in point-of-care testing at home, small clinics, or underdeveloped regions.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Wu-Shang Yang,Ze-Lin Qiu,Wen-Han Yu,Li-Feng Fang,Bao-Ku Zhu
Nanofiltration membrane (NF) have become increasingly crucial in ion separation. However, traditional polyamide (PA) NF suffers from poor selectivity for mono-/divalent anions due to weak charges and structure defects. Thus, a novel binary organic phosphonic acid, alendronic acid (AA), was firstly used as an aqueous co-monomer via interfacial polymerization (IP) to create negatively charged nanofiltration membranes with both higher charge density and pore size homogeneity for the separation of mono-/divalent anions. Both structure characterization and performance tests demonstrated the successful introduction of AA in the separation layer. Compared with pristine membrane, the NaCl/Na 2 SO 4 selectivity of membrane incorporated with AA was four times improved, while the permeability exhibited no decline. Simultaneously, the separation efficiency of Cl - /SO 4 2- was tested in NaCl/Na 2 SO 4 binary mixed solutions with varying total concentrations and different mass ratios, and the separation factor consistently exceeded 500 across a concentration range of 1 g/L to 20 g/L. Moreover, the AA-integrated membrane exhibited excellent chlorine resistance after 20000 ppm·h of chlorine treatment, which is promising for high efficiency separation of mono-/divalent anions in industrial applications.
研究领域:
材料科学
·
环境工程
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 9
·期 2
·508-522
·
IF 5.344
Lei Chen,Yufei Tang,Kang Zhao,Xiang Zha,Min Wei,Quanchang Tan,Zixiang Wu
Drugs are loaded into PMMA bone cement to reduce the risk of infection in freshly implanted prostheses or to promote the differentiation and growth of osteoblasts. However, the same method of loading of drugs in the bone cement cannot simultaneously achieve an effective antibacterial response and long-term treatment outcomes for osteoporosis based on a patient's clinical needs. In the present study, gentamicin sulfate (GS)/alendronate (ALN)-dual-loaded gelatin modified PMMA bone cement (GAPBC) was fabricated to provide rapid and continuous antibiotic release and long-term anti-osteoporotic therapy. Specifically, the gelatin microspheres were loaded with the drugs using separate methodologies, namely, ALN was loaded during fabrication of the gelatin microspheres after which GS was absorbed onto the gelatin from solution. The results demonstrate that sequential release of the GS and ALN was achieved, GS release playing a major role over the first 24 hours and ALN release dominant after 3 weeks of immersion in PBS, resulting from the graded distribution within the gelatin microspheres, and the final drug release ratio of GS (73.6%) and ALN (68.5%) from the modified bone cement was significantly higher than from PMMA bone cement. Therefore, GAPBC represents a potential drug carrier for future clinical applications.
研究领域:
生物医学工程
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药剂学
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骨科研究
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Hongyu Quan,Yuwei He,Jujiang Sun,Weihu Yang,Wei Luo,Ce Dou,Fei Kang,Chunrong Zhao,Jian He,Xiaochao Yang,Shiwu Dong,Hong Jiang
Bone defects/fractures are common in older people suffering from osteoporosis. Traditional hydroxyapatite (HA) materials for osteoporotic bone repair face many challenges, including limited bone formation and aseptic loosening of orthopedic implants. In this study, a new multifunctional HA is synthesized by spontaneous assembly of alendronate (AL) and Fe3O4 onto HA nanocrystals for osteoporotic bone regeneration. The chemical coordination of AL and Fe3O4 with HA does not induce lattice deformation, resulting in a functionalized HA (Func-HA) with proper magnetic property and controlled release manner. The Func-HA nanocrystals have been encapsulated in polymer substrates to further investigate their osteogenic capability. In vitro and in vivo evaluations reveal that both AL and Fe3O4, especially the combination of two functional groups on HA, can inhibit osteoclastic activity and promote osteoblast proliferation and differentiation, as well as enhance implant osseointegration and accelerate bone remodeling under osteoporotic condition. The as-developed Func-HA with coordinating antiresorptive ability, magnetic property, and osteoconductivity might be a desirable biomaterial for osteoporotic bone defect/fracture treatment.
研究领域:
生物材料学
·
骨科医学
·
纳米医学
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DOI
PubMed
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2018
·
IF 3.026
Xue Dong,Shaohua Zou,Chunjing Guo,Kaili Wang,Feng Zhao,Huaying Fan,Jungang Yin,Daquan Chen
The traditional therapy of cancer has systemic side effects, and many cancers, such as human breast cancer and lung cancer easily metastasize to bones, leading to the formation of secondary tumours. This study was aimed at enhancing the anti-tumour effect of curcumin (CUR) and preventing tumour spread to the bone. A novel multifunctional redox-responsive and CD44 receptor targeting polymer-drug, poly alendronate-hyaluronan-S-S-curcumin copolymer (ALN-oHA-S-S-CUR) based CUR and alendronate (ALN) were synthesized successfully with the disulphide bond linker. The structure of ALN-oHA-S-S-CUR was characterized by 1H-NMR. The nanomedicine had natural anti-tumour drugs (CUR) as the hydrophobic kernel, and targeting CD44 receptor oligosaccharides of hyaluronan (oHA) and other anti-tumour drugs (ALN) as hydrophilic shell, named ALN-oHA-S-S-CUR conjugates, which could self-assemble into micelle-like nano-spheres in water via a dialysis method with hydrodynamic diameters of 179 ± 23 nm. Interestingly, the cur-loaded ALN-oHA-S-S-CUR micelles were stable in PBS but were capable of releasing the drug under the reducing environment. The rate of drug release was proportional to the GSH concentration. The uptake and cytotoxicity of micelles were higher in MDA-MB-231 cells than in MCF-7 cells because of a higher expression of the CD44 receptor in the former cell line. And compared to the cur-loaded oHA-CUR micelles, the cur-loaded ALN-oHA-S-S-CUR micelles had a good cellular uptake in 2D cancer cell and penetrability in 3D cancer cell spheroids. These results indicated the active targeting redox-sensitive micelles were promising as intracellular drug delivery systems for cancer treatment.
研究领域:
药学
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肿瘤学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2024
·
IF 18
Lingfei Zhao,Chenyu Liu,Xing Chen,Zirui He,Shuiquan Zhang,Anan Zhang,Shuaimin Tang,Zihan Wu,Changsheng Liu,Yuan Yuan
The osteoporotic bone defect caused by excessive activity of osteoclasts has posed a challenge for public healthcare. However, most existing bioinert bone cement fails to effectively regulate the pathological bone microenvironment and reconstruct bone homeostasis in the presence of osteoclast overactivity and osteoblast suppression. Herein, inspired by natural bone tissue, an in-situ modulation system for osteoporotic bone regeneration is developed by fabricating an injectable double-crosslinked PEGylated poly(glycerol sebacate) (PEGS)/calcium phosphate cement (CPC) loaded with sodium alendronate (ALN) (PEGS/CPC@ALN) adhesive bone cement. By incorporating ALN, the organic-inorganic interconnection within PEGS/CPC@ALN results in a 100 % increase in compression modulus and energy dissipation efficiency. Additionally, PEGS/CPC@ALN effectively adheres to the bone by bonding with amine and calcium ions present on the bone surface. Moreover, this in-situ regulation system comprehensively mitigates excessive bone resorption through the buffering effect of CPC to improve the acidic microenvironment of osteoporotic bone and the release of ALN to inhibit hyperactive osteoclasts, and facilitates stem cell proliferation and differentiation into osteoblasts through calcium ion release. Overall, the PEGS/CPC@ALN effectively regulates the pathological microenvironment of osteoporosis while promoting bone regeneration through synergistic effects of drugs and materials, thereby improving bone homeostasis and enabling minimally invasive treatment for osteoporotic defects.
研究领域:
生物材料学
·
骨科医学
·
再生医学
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DOI
PubMed
高影响因子
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