Food Bioscience
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2026
·
IF 6.2
Shiqin Gong,Ziqi Li,Fang Liu,Yi Xiao
High-sugar diets are associated with multiple health risks, such as obesity, metabolic disorders, and cardiovascular diseases. To mitigate the adverse effects of high sugar intake on biological health, aspartame (ASP) is widely used as one of the sugar substitutes. In this study, we used Caenorhabditis elegans ( C. elegans ) as the model to reveal the specific mechanism by which ASP affects healthy lifespan. Our results showed that ASP (1, 10, 100 μmol/L) significantly extended the lifespan of C. elegans and improved health indicators in dose dependent manner, including reduced lipofuscin accumulation and enhanced motility, but did not influence the fertility of worms. Furthermore, by screening the classic signaling pathways, we discovered ASP extended healthspan via mechanistic target of rapamycin complex 1 (mTORC1) pathway. In addition, we found that ASP inhibited mTORC1 activity to promote the nuclear translocation of helix-loop-helix protein 30 (HLH-30), the nematode homolog of transcription factor EB (TFEB), and subsequently upregulated autophagy-related genes such as autophagy-related gene 5 ( atg-5 ), beclin 1 ( bec-1 ), LC3, GABARAP, GATE-16 family member 1 ( lgg-1 ) and LC3, GABARAP, GATE-16 family member 2 ( Lgg-2 ). This ultimately activated the autophagy process, thereby delaying aging. This finding elucidates that ASP induces autophagy to enhance healthspan in C. elegans dependent on the mTORC1/TFEB pathway, offering a novel approach for anti-aging research.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Yan Bai,Ji Feng,Xiao-Yu Wu,Zhi-Sheng Yao,Yue-Tong Liu,Yu-Hong Li,Guo-Dong Lu,Kun Xue
Background Aspartame, a widely used non-nutritive sweetener, has been associated with potential neurotoxicity. However, it remains unclear whether aspartame consumption can exacerbate cerebral ischemia–reperfusion injury (CIRI), a major contributor to stroke outcomes, and the underlying mechanisms are poorly understood. Methods In vivo , male C57BL/6 J mice were given free access to 0.1% or 0.2% (w/v, equivalent to human acceptable daily intake level) aspartame, for 7 days, followed by bilateral common carotid artery occlusion (BCCAO) to induce CIRI. In vitro , hippocampal neural stem cells (NSCs) were subjected to oxygen–glucose deprivation (OGD) with aspartame. Cell injury, general and mitochondrial reactive oxygen species (ROS) burden, and mitochondrial function were assessed. Gene Ontology (GO), KEGG enrichment, and protein–protein interaction (PPI) network analyses were performed to identify potential targets. The ERK/CREB1 signaling pathway was evaluated by western blotting and pharmacological modulation. Results Aspartame significantly increased the infarct volume and aggravated neuronal damage in BCCAO-treated mice. In NSCs, aspartame, but not acesulfame or sucralose, selectively enhanced OGD-induced apoptosis, accompanied by mitochondrial depolarization and excessive ROS accumulation, while showing minimal effects under normoxia conditions. GO/KEGG and PPI analyses highlighted ERK/CREB1 as an important node in aspartame-induced neurotoxicity. Consistently, aspartame suppressed the phosphorylation of ERK1/2 and CREB1. The ERK activator LM22B-10 and mitochondria-targeted antioxidant Mito-TEMPO partially reversed mitochondrial dysfunction, apoptosis and ERK/CREB1 suppression. Additionally, aspartame increased the mRNA expression of pro-inflammatory cytokines (TNF- α , IL-1 β and IL-6) and increased NF-κB p65 phosphorylation and reduced the proportion of Tuj1-positive cells, which were mitigated by ERK activation. Conclusion Aspartame exacerbates CIRI-associated injury in a stress-dependent manner, involving mitochondrial dysfunction, ROS accumulation, and ERK/CREB1 suppression. Future studies are warranted to explore the long-term neurobehavioral outcomes and validate these mechanisms in clinical scenarios.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2022
·Vol. 25
·期 3
·1649-1658
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IF 3.945
Jun Dong,Haoran Wu,Yi Cao,Jiaxin Yuan,Qingyan Han,Wei Gao,Chengyun Zhang,Jianxia Qi,Mengtao Sun
In this study, a capillary device based on the surface plasmon-enhanced Raman scattering effect was prepared by a simple and easy method. First, the capillary was treated with APTES solution. Due to the electrostatic effect, gold nanoparticles could be easily and tightly assembled in the capillary inner wall. On this basis, the effects of changing the concentration of APTES, the concentration of colloids and the soaking time of the capillary in the colloids on the assembly of gold nanoparticles on the inner wall of the capillary were studied, and the SERS enhancement effect under different conditions was analyzed, and the optimal solution was successfully found. At the same time, the reason why the capillary substrate shows better SERS performance than the traditional planar substrate is deeply discussed. Since the nanoparticles can be attached to the upper and lower surfaces of the inner wall of the capillary, the utilization rate of nanoparticles and laser is improved, thereby achieving higher enhancement. For the detection of the probe molecule rhodamine 6G, it was proved that the substrate has good uniformity and the lowest detection limit can reach 10−10 M. Finally, the real-life pesticide thiram and the food additive aspartame were tested, and the detection limits could reach 10−6 M and 0.25 g L−1. It is confirmed that the prepared capillary shows excellent SERS performance and can be used for rapid detection in various fields.
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FOOD AND AGRICULTURAL IMMUNOLOGY
·
2023
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IF 3
Linyang Song,Xiaoyi He,Mengxia Wang,Lixia Li,Jing Liu,Guoying Li,Junhua Yang
People often simultaneously expose to acceptable/tolerable daily intake of food additives and pesticides in daily life. Exposure in adult rodents to acceptable/tolerable daily intake of food additives and pesticides chemical mixtures (AFACM) is reported to cause health damage. Our recently study reported that AFACM exposure of pregnant mice resulted in neuroinflammation and abnormal emotional behaviours in offspring. Here, we found that neutralising IFN-γ with neutralising antibodies completely blocked the influences induced by maternal AFACM exposure, including the increased IFN-γ level in serum, increased IFN-γ, interleukin-6 and tumour necrosis factor-α levels in prefrontal cortex(PFC) in offspring on postnatal day(PND)7, the abnormal emotional behaviours in offspring on PND56, and the decreased of ATPase phospholipid flippase 8A2(ATP8A2) in the PFC of offspring on PND7. In sum, maternal AFACM exposure induced the PFC neuroinflammation, abnormal emotional behaviours and decreased PFC ATP8A2 expression through increasing systemic IFN-γ in offspring.
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ENVIRONMENTAL POLLUTION
·
2023
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IF 8.9
Zhijian Ke,Junyun Song,Jingrui Ma,Mengting Wang,Haiguang Mao,Chunli Xia,Lili Qi,Yidong Zhou,Jinbo Wang
Aspartame is one of the main varieties of artificial sweeteners. Although it has been approved as a food additive , the environmental hazards and ecological risks posed by aspartame are attracting more and more attention. In the present study, strain Pseudarthrobacter sp. AS-1 was isolated and characterized as an efficient aspartame degrader. Strain AS-1 was capable of degrading 200 mg L −1 aspartame within 10 h under conditions optimized at 30 °C and pH 8.0. At the same time, it was found that enzymes degrading aspartame in strain AS-1 were induced and secreted extracellularly. Degradation of aspartame in Pseudarthrobacter sp. AS-1 was identified as following: it was first demethylated to aspartyl-phenylalanine, then degraded to phenylalanine and aspartate , and finally the two amino acids were further degraded. In addition, strain AS-1 was able to remove more than 85% of aspartame in soil and river water. It is the first time that pure bacterial cultures were reported to have the capability of aspartame degradation. These findings add to our knowledge of the microbial metabolic mechanisms of aspartame.
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Biology-Basel
·
2021
·Vol. 10
·期 6
·548
·
IF 5.079
Ferry Saputra,Yu-Heng Lai,Rey Arturo T. Fernandez,Allan Patrick G. Macabeo,Hong-Thih Lai,Jong-Chin Huang,Chung-Der Hsiao
Simple SummaryThe usage of artificial sweetener has been increased from year to year as the result of pursuing healthy lifestyle. However, ironically, several studies suggest that the consumption of artificial sweeteners cause sugar-related adverse effects (e.g., obesity, type 2 diabetes and cardiovascular disease). In this study, we explore the potential cardiovascular adverse effect of several artificial sweeteners using zebrafish as animal model. We found that artificial sweetener at the highest concentration found in nature only slightly alter the cardiovascular performance of zebrafish larvae. Furthermore, no alteration of cardiac performance showed after longer incubation which support the safety of artificial sweeteners.AbstractArtificial sweeteners are widely used food ingredients in beverages and drinks to lower calorie intake which in turn helps prevent lifestyle diseases such as obesity. However, as their popularity has increased, the release of artificial sweetener to the aquatic environment has also increased at a tremendous rate. Thus, our study aims to systematically explore the potential cardiovascular physiology alterations caused by eight commercial artificial sweeteners, including acesulfame-K, alitame, aspartame, sodium cyclamate, dulcin, neotame, saccharine and sucralose, at the highest environmentally relevant concentration on cardiovascular performance using zebrafish (Danio rerio) as a model system. Embryonic zebrafish were exposed to the eight artificial sweeteners at 100 ppb and their cardiovascular performance (heart rate, ejection fraction, fractional shortening, stroke volume, cardiac output, heartbeat variability, and blood flow velocity) was measured and compared. Overall, our finding supports the safety of artificial sweetener exposure. However, several finding like a significant increase in the heart rate and heart rate variability after incubation in several artificial sweeteners are noteworthy. Biomarker testing also revealed that saccharine significantly increase the dopamine level in zebrafish larvae, which is might be the reason for the cardiac physiology changes observed after saccharine exposure.Keywords:artificial sweeteners;zebrafish;cardiac performance
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TALANTA
·
2021
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IF 6.057
Qichao Ye,Chen Men,Lili Tian,Yuxin Liu,Lei Zhan,Yuan Fang Li,Cheng Zhi Huang,Shu Jun Zhen
Conventional methods for the detection of the sulfur mustard poisoning metabolic marker, thiodiglycol (TDG), require expensive instruments and reagents as well as professional operators. To address these problems, a novel test strip based on a molecularly imprinted sensitive membrane (MIM) was developed in this work for point-of-care (POC) detection of TDG. The TDG test strip was prepared conveniently by coating molecular imprinted polymers (MIPs) on a nitrocellulose membrane. When the sample contained TDG, the MIPs could specifically bind with TDG. A great number of AuNPs (AuNPs) could then be adsorbed on the test strip via the formation of an Au–S bond between TDG and AuNPs, giving the test strip the obvious red color of AuNPs. In the absence of TDG, the test strip exhibited much lighter color because it could not adsorb AuNPs. By monitoring the color change of the test strip, TDG could be detected from 1.0 ng/mL to 100.0 μg/mL with a detection limit of 0.23 ng/mL (3σ) under the optimal conditions (the molar ratio of TDG to MAA was 1:2; the eluent was chloroform; the elution time was 50 min; the reaction time between MIPs and TDG was 15 min; the adsorption time of AuNPs was 40 min; the temperature of the reaction system was 35 °C). This method has excellent selectivity and has been used to detect TDG in urine, showing great potential for POC detection of TDG in clinical samples.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
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IF 2.88
Hongmei Zhang,Shixin Sun,Yanqing Wang,Zhenghao Fei,Jian Cao
In this work, the interactions between bovine serum albumin (BSA) and five sweeteners including aspartame (APM), acesulfame (AK), sucralose (TGS), sodium cyclamate (SC), and rebaudioside-A (REB-A) have been studied by multispectroscopic techniques, and molecular simulation in order to provide much useful information for the application of new and safer artificial sweeteners. Fluorescence quenching assays indicated that the formation of complexes between sweeteners and BSA mainly induced the fluorescence quenching of protein and the binding site number were about 1 indicting that there is one mainly binding site of APM, AK, TGS, SC, or REB-A in domain of BSA with relatively weak interactions. Molecular modeling results indicated that hydrogen bonding interactions were the mainly binding forces of sweeteners with BSA. Circular dichroism spectra indicated that APM and REB-A obviously induced the secondary structure changes of BSA. The presence of APM increased the fraction of α–Helix of BSA from 65.4% to 73.8%, while the presence of REB-A resulted in decreasing the fraction of α–helix of BSA from 65.4% to 51.2%. The melting temperature studies showed that these five sweeteners except REB-A act as stabilizers to increase the thermal stability of BSA during the thermal denaturation process. In addition, AK, TGS, and SC obviously increased the esterase-like activity of BSA, and such loss of activity of BSA induced by APM and REB-A.
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Food Analytical Methods
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2018
·Vol. 12
·期 2
·381-393
·
IF 2.245
Sun Xiao-Dong,Wu Hai-Long,Liu Zhi,Chen Yue,Liu Qian,Ding Yu-Jie,Yu Ru-Qin
A rapid and sensitive analytical strategy combining high-performance liquid chromatography-diode array detection (HPLC-DAD) and chemometrics methods was developed for the determination of multi-class food additives in a variety of beverage samples. Different kinds of beverages, which contain diverse unknown interferences, can be directly injected into a chromatographic system after a simple dilution or pretreatment step, and the data were recorded in a short time under a simple gradient elution mode. Although peaks overlapped and changeable interferences existed in the dimension of the chromatographic and spectral in real beverages, all food additives were accurately resolved by using a second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm, and accurate chromatographic profiles, spectral profiles, and concentration profiles were also obtained. Limits of detection for all additives were between 1.40 and 165.1 ng mL −1 , while limits of quantitation ranged from 4.20 to 500.2 ng mL −1 . The spiked recoveries were in the range of 87.3–103% (except amaranth) with RSDs less than 10.2%. Compared with the results obtained by classic HPLC method, our proposed method was more accurate. In all, the proposed method is fast, sensitive, and universal and could be used as a reliable tool to determine food additives and quality monitoring in different complex beverages.
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TALANTA
·
2018
·
IF 4.244
Li’na Qiao,Sihua Qian,Yuhui Wang,Hengwei Lin
Pesticides play a critical role in improving crop yield in modern agriculture, but their residues significantly harm the environment and human health. Herein, a novel and simple colorimetric sensor array built on sulfuric acid assisted KMnO 4 fading strategy has been developed for pesticides detection and discrimination. This sensor array is facilely fabricated by KMnO 4 and sulfuric acid through simply adjusting their concentrations and ratios. Hierarchical clustering analysis (HCA) demonstrates that the as-fabricated colorimetric sensor array has a high dimensionality, and shows excellent capability to recognize common kinds of pesticides from potential interferants. Semi-quantitative detection was achieved through combining HCA and corresponding fitting curves. Moreover, the proposed sensor array was successfully applied to detect pesticide residues (e.g. carbaryl) in real samples. The strategy described herein will not only “maximally” simplify the design and fabrication approach, but expand the application fields of colorimetric sensor array methodology towards weak-reactive analytes.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Hongkun Zhao,Chunning Chen,Yalei Wang,Jiaqi Liu,Jiaxin Lu,Jingtong Zhai,Rui Li,Nan Lu
For on-site analysis, the combination of surface enhanced Raman scattering (SERS) and colorimetry, as a dual-mode detection, can effectively improve the accuracy of detection, and reduce the influence of instrument fluctuation, which greatly improves the accuracy and reliability of the results. However, the preparation of SERS/colorimetry substrates are usually time-consuming and costly, which limits their practical applications. In this paper, a hydrophobic paper-based SERS/colorimetry substrate can be prepared by a simple spraying method. The hydrophobicity is introduced by the structures formed with polydimethylsiloxane and polymethylmethacrylate, which leads to high detection sensitivity due to its enrichment effect. Moreover, the electrostatic interaction between Ag nanoparticles and the analytes further enhances the performance of SERS and colorimetry in detection of thiram and aspartame. It also provides a new method for the detection of aspartame with colorimetry. Finally, the detection limits of SERS and colorimetry for thiram and aspartame are 0.1 mg/L and 0.1 g/L, 1 mg/L and 0.1 g/L, respectively. The paper-based SERS/colorimetry substrate makes the results more reliable through dual-mode detection, which shows great potential in the detection of real samples.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Weiwei Jiang,Yuwei Cheng,Lei Hou,Ying Huang,Sizhen Wang,Yunchang Zhang,Tao Jiang,Feng Yang,Zhiqiang Ma
The challenges posed by intractable relapse and metastasis in cancer treatment have led to the development of various forms of photodynamic therapy (PDT). However, traditional drug delivery systems, such as virus vectors, liposomes, and polymers, often suffer from issues like desynchronized drug release, carrier instability, and drug leakage during circulation. To address these problems, we have developed a dual-prodrug nanogel (PVBN) consisting of Pyro (Pyropheophorbide a) and SAHA (Vorinostat) bound to BSA (Bovine Serum Albumin), which facilitates synchronous and spontaneous drug release in situ within the lysosome. Detailed results indicate that PVBN-treated tumor cells exhibit elevated levels of ROS and Acetyl-H3, leading to necrosis, apoptosis, and cell cycle arrest, with PDT playing a dominant role in the synergistic therapeutic effect. Furthermore, the anti-tumor efficacy of PVBN was validated in melanoma-bearing mice, where it significantly inhibited tumor growth and pulmonary metastasis. Overall, our dual-prodrug nanogel, formed by the binding of SAHA and Pyro to BSA and releasing drugs within the lysosome, represents a novel and promising strategy for enhancing the clinical efficacy of photochemotherapy.
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