SEPARATION AND PURIFICATION TECHNOLOGY
·
2019
·
IF 5.107
Dongze Wang,Gang Chen,Xuemei Li,Qiong Jia
A β -cyclodextrin based hypercrosslinked polymer (Bn-CD-HCP) was prepared using 4,4′-bis(chloromethyl)-1,1′-biphenyl as the crosslinker via a Friedel-Crafts reaction. The Bn-CD-HCP material showed high surface area and thermal stability, making it an efficient adsorbent for albendazole, a benzimidazole carbamate. 8 was determined to be the best pH value for albendazole adsorption and the time necessary to reach equilibrium of adsorption was 30 min. The adsorption process agreed with the pseudo-second-order model and Langmuir isotherm model. A high adsorption capacity of 181.82 mg g −1 was achieved. The negative value of Gibbs free energy change suggested a spontaneous adsorption process. In addition, Bn-CD-HCP could be reused for albendazole adsorption for multiple cycles without significant losing its adsorption amount.
研究领域:
环境科学
·
材料科学
·
药学
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产品关联
DOI
高影响因子
LIFE SCIENCES
·
2022
·
IF 6.78
Tianfeng Yang,Cheng Cheng,Rui Xu,Jian Huo,Xiujuan Peng,Yanbin Chen,Yonghong Liang,Zhiheng Su,Yanmin Zhang
Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third most common cause of cancer-related deaths. The WW-domain containing oxidoreductase (WWOX) protein suppresses carcinogenesis and its absence is closely related to aggressive HCC phenotypes. In this study, by using SPR analysis, cell viability assay and xenograft mice models, we found that albendazole (ABZ), a safe and effective anthelmintic drug, exhibited the binding affinity with WWOX protein and potential inhibition effect on HCC cells in vitro and in vivo . Overexpression and knockdown of WWOX confirmed that the suppression of HCC by ABZ. Flow cytometric analysis, western blotting analysis and Co-IP were conducted to study the mechanism of ABZ. Our data showed that ABZ regulated the interaction between WWOX and its binding proteins including p53 and C-MYC. Furthermore, ABZ triggered p53-induced intrinsic apoptosis and suppressed EMT-mediated migration by C-MYC/Fibronectin axis. In addition, ∆NP73 expression was significantly inhibited by ABZ, which further sensitized p53-induced intrinsic apoptosis and cell cycle arrest. In summary, ABZ could suppress the proliferation and migration of HCC cells by regulating WWOX-dependent signaling pathway.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·614-620
·
IF 1.938
Qin Li,Xuanping Tan,Lingli Fu,Qu Liu,Weiwei Tang
Glutathione (GSH)-capped CdTe QDs with diameters of 3 nm were synthesised. The interaction between the QDs and albendazole (ABZ) was investigated by UV-vis absorption, resonance Rayleigh scattering (RRS) and fluorescence spectroscopy. Based on changes in the UV-vis absorption spectra of QDs and ABZ, we demonstrated that the QDs associated with ABZ to form new complexes. In pH 7.1 PBS buffer solution, the fluorescence intensity of QDs was effectively quenched by ABZ. The fluorescence quenching of QDs by ABZ mainly results from the QDs–ABZ complexes, and the electrostatic attraction played a major role in stabilising these complexes. Furthermore, the interaction between QDs and ABZ led to a remarkable enhancement in RRS, which was proportional to the ABZ concentration within a certain concentration range. The suitable reaction conditions, important factors as well as the influence of coexisting substances were studied. The possible reaction mechanism was also discussed.
研究领域:
分析化学
·
生物医学
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产品关联
DOI
高影响因子
AAPS PHARMSCITECH
·
2025
·Vol. 26
·期 1
·1-13
·
IF 4
Lan Sumin,Chen Kexi,Feng Liqiang,Sima Panle,Ji Xiaoyao,Wu Feihua,Lin Yining
Albendazole serves as a broad-spectrum anthelmintic medication for treating hydatid cysts and neurocysticercosis. However, its therapeutic effectiveness is limited by poor solubility. Nanocrystals offer a promising technology to address this limitation by enhancing drug solubility. The objective of this study is to evaluate an effective stabilizer for creating an albendazole nanocrystal formulation to improve oral absorption. Among different surfactants and polymers examined, tea saponins were used as the stabilizer to develop a nanosuspension with the particle size of 180 nm through a wet grinding approach. The physical characteristics of the nanocrystals were assessed using SEM, DSC, and XRPD. The nanocrystals significantly enhanced solubility by 2.9–2602 fold in different media and showed significant enhancement in dissolution rate compared to albendazole crystals in both pH 1.0 and pH 6.8 medium. Everted gut sacs experiments demonstrated that the nanocrystals increased P app by 3.60-fold in duodenum, 3.76-fold in jejunum, 3.71-fold in ileum, and 5.26-fold in colon, respectively. Furthermore, pharmacokinetic studies revealed that the nanocrystals significantly enhanced oral bioavailability, resulting in a 4.65-fold increase in plasma AUC 0−t value of albendazole sulfoxide (the primary active metabolite of albendazole) compared to the albendazole group. The present data indicates that tea saponins are potential natural stabilizers for preparing nanocrystals with enhanced oral bioavailability for insoluble drugs. Graphical
研究领域:
药剂学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2019
·
IF 5.107
Dongze Wang,Xuemei Li,Xiangqun Jin,Qiong Jia
In the present study, cucurbit[6]uril-based hypercrosslinked polymers (HCPs) were successfully synthesized and employed for the capture of albendazole, a broad-spectrum anthelmintic drug. Fourier-transform infrared spectroscopy, elemental analysis, powder X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, and N 2 adsorption-desorption measurements were used to prove the successful preparation of the desired HCPs. Thanks to the encapsulation of cucurbit[6]uril toward albendazole and the high surface area of HCPs, remarkable adsorption capacity of albendazole was achieved. The adsorption behavior followed Langmuir adsorption isotherm with a negative value of Gibbs free energy change, suggesting a spontaneous adsorption process. In addition, the kinetics of albendazole adsorption was investigated, which could be described with the pseudo-second-order kinetics model.
研究领域:
材料科学
·
药物化学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 6
·2824-2837
·
IF 4.1
Yiting Wen,Yujia Zhang,Xiaoli Zhang,Linjun Wang,Qiuxia Pan,Qiuhan Bai,Du Zhu,Weiming Chai
BACKGROUND Tyrosinase is the key enzyme involved in enzymatic browning of plant-derived foods. Inhibition of tyrosinase activity contributes to the control of food browning. Due to safety regulations or other issues, most identified tyrosinase inhibitors are not suitable for practical use. Therefore, it is necessary to search for novel tyrosinase inhibitors. In this study, the anti-tyrosinase activity and mechanism of albendazole and 2-(2-aminophenyl)-1 H -benzimidazole (2-2-A-1HB) were investigated through ultraviolet–visible absorption spectroscopy, fluorescence spectra, molecular docking, and molecular dynamic (MD) simulation. The anti-browning effect of albendazole on fresh-cut apples was then elucidated. RESULTS Albendazole and 2-2-A-1HB were both efficient tyrosinase inhibitors with IC 50 of 51 ± 1.5 and 128 ± 1.3 μmol L −1 , respectively. Albendazole suppressed tyrosinase non-competitively and formed tyrosinase–albendazole complex statically. Hydrogen bond and hydrophobic interaction were major driving forces in stabilizing the tyrosinase–albendazole complex. While 2-2-A-1HB inhibited the enzyme competitively and quenched its intrinsic fluorescence through a static mechanism, it generated strong binding affinity with tyrosinase through hydrophobic interaction. MD simulations further validated that albendazole/2-2-A-1HB could form stable complexes with tyrosinase and loosened its basic framework structure, leading to a change in secondary structure and conformation. In addition, albendazole could delay the browning of fresh-cut apples by inhibiting the activity of polyphenol oxidase, peroxidase and phenylalanine ammonia-lyase, and reducing the oxidation of phenolic compounds. CONCLUSION This research might provide a deep view of tyrosinase inhibition by benzimidazole derivatives and a theoretical basis for developing albendazole as a potential fresh-keeping agent. © 2023 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 23
·3894
·
IF 5.561
Yawen Guo,Zhaoyuan He,Yali Zhu,Shuyu Liu,Pengfei Gao,Kaizhou Xie,Tao Zhang,Yuhao Dong
An innovative and sensitive approach using high-performance liquid chromatography-photo diode array detection (HPLC-PDAD) was developed and optimized for the simultaneous determination of abamectin (ABM), ivermectin (IVM), albendazole (ABZ) and three metabolites in eggs. The samples were extracted with acetonitrile (MeCN)/water (90:10,v/v), and the extracts containing the targets were cleaned up and concentrated by a series of liquid–liquid extraction (LLE) steps. A reversed-phase C18 column and a mobile phase consisting of 0.1% trifluoroacetic acid (TFA) aqueous solution and methanol (MeOH) were utilized to perform optimal chromatographic separation. The developed method was validated on the basis of international guidelines. The limits of detection (LODs) and quantitation (LOQs) were 2.1–10.5 µg/kg and 7.8–28.4 µg/kg, respectively. Satisfactory linear relationships were observed for the targets in their corresponding concentration ranges. The mean recoveries ranged from 85.7% to 97.21% at 4 addition levels, with intraday and interday relative standard deviations (RSDs) in the ranges of 1.68–4.77% and 1.74–5.31%, respectively. The presented protocol was demonstrated to be applicable and reliable by being applied for the detection of target residues in locally sourced egg samples.Keywords:abamectin;ivermectin;albendazole;HPLC–PDAD;LLE
研究领域:
分析化学
·
兽医学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
ANTI-CANCER DRUGS
·
2021
·Vol. 33
·期 1
·19
·
IF 2.248
Liu Shengli,Liu Honglian,Sun Hao,Deng Shengming,Yue Ling,Weng Zhen,Yang Jianfeng,Zuo Bin,He Yang,Zhang Bin
Albendazole (ABZ), a clinical antiparasitic drug, has shown potential antitumor effects in various tumors. Herein, we prepared dimeric cRGD [(cRGD) 2 ] modified human serum albumin (HSA) nanosystem to co-delivery of albendazole (ABZ) and iodine-131 ( 131 I) for chemoradiotherapy of triple-negative breast cancer (TNBC). [email protected] NPs were synthesized by the self-assembly method. 131 I-(cRGD) 2 / [email protected] NPs were fabricated through covalently binding [email protected] NPs with (cRGD) 2 peptides, followed by chloramine T direct labeling with 131 I. In vitro therapeutic effects on TNBC (MDA-MB-231 and 4T1 cells) were determined using MTT assay, crystal violet assay, wound-healing assay and western blotting analysis. In vivo treatment was performed using 4T1-bearing mice, and the tumor-targeting efficacy was assessed by gamma imaging. The distribution of NPs was quantitatively analyzed by detecting the gamma counts in tumor and main organs. The nanoparticles possessed negative charge, moderate size and good polydispersity index. Dual responding to pH and redox, the in vitro release rate of ABZ was more than 80% in 72 h. In vitro, NPs inhibited the proliferation of TNBC cells in a concentration-dependent manner and decreased cell migration. Western blotting analysis showed that the NPs, as well as free ABZ, cell-dependently induced autophagy and apoptosis by restraining or promoting the expression of p-p38 and p-JNK MAPK. In vivo, gamma imaging exhibited an earlier and denser radioactivity accumulation in tumor of 131 I-(cRGD) 2 / [email protected] NPs compared to NPs free of (cRGD) 2 conjugating. Furthermore, 131 I-(cRGD) 2 / [email protected] NPs significantly suppressed tumor growth by restraining proliferation and promoting apoptosis in vivo. Our study suggested that the nanoparticles we developed enhanced tumor-targeting of ABZ and increased antitumor effects by combination of chemotherapy and radiotherapy.
研究领域:
肿瘤学
·
药学
·
纳米技术
·
癌症治疗
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 19
·5247-5259
·
IF 3.125
Pan Ziyu,Peng Jingdong,Zang Xu,Lei Gang,He Yan,Liu Di
A novel, highly selective, and sensitive resonance light scattering (RLS) detection approach coupled with high performance liquid chromatography (HPLC) was researched and developed for the synchronous analysis of three kinds of benzimidazole anthelmintics, including mebendazole (MBZ), albendazole (ABZ), and fenbendazole (FBZ) for the first time. In the pH range of 3.5–3.7 Britton-Robinson buffer medium, three kinds of anthelmintics, which were separated by HPLC, reacted with eosin Y (EY) to form 1:1 ion-association complexes, resulting in significantly enhanced RLS signals and the maximum peak located at 335 nm. The enhanced RLS intensity was in proportion to the MBZ, ABZ, and FBZ concentration in the range 0.2–25, 0.2–23, and 0.15–20 μg/mL, respectively. The limit of detection was in the range of 0.064–0.16 μg/mL. In addition, human urine was determined to validate the proposed method by spiked samples and real urine samples. Satisfactory results were obtained by HPLC-RLS method.
The diagram mechanism of generating resonance between emitted light and scattered light
研究领域:
分析化学
·
药物分析
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhaoyuan He,Xinrui Shi,Yawen Guo,Fanxun Guan,Pengfei Gao,Yayun Tang,Shuyu Liu,Yali Zhu,Kaizhou Xie,Hailan Chen
Antimicrobial drugs are frequently used in a combination or shuttle way to cope with coinfection of bacteria or parasites and prevent drug resistance, thus the accurate quantification of multiple drug residues in animal-derived foods is crucial to ensure food safety. Here, a simple and efficient high-performance liquid chromatography-photodiode array (HPLC-PDA) method was established for the simultaneous quantitative screening of six common residues of antiparasitic drugs, including abamectin (ABM), ivermectin (IVM), albendazole (ABZ) and the three metabolites of ABZ in beef and chicken. The LODs and LOQs for six target compounds in beef and chicken are determined to be 3.2 to 12.5 µg/kg and 9.0 to 30.0 µg/kg, respectively. The calibration curves show good linearity (R 2 ≥ 0.9990) between the peak area and concentration. The recoveries from the fortified blank samples are all above 85.10%. Finally, the applicability of the HPLC-PDA method is successfully demonstrated by the real sample analysis.
研究领域:
分析化学
·
食品科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NPG Asia Materials
·
2019
·Vol. 11
·期 1
·1-18
·
IF 8.052
Liang Jianming,Zhu Ying,Gao Caifang,Ling Chengli,Qin Jing,Wang Qi,Huang Yongzhuo,Lu Weigen,Wang Jianxin
Current chemotherapeutics for glioma are not sufficiently effective due to their low tolerance and poor blood–brain barrier (BBB) permeability. Energy restriction based on co-delivery of albendazole (Abz) and nano-silver has been proven to suppress tumor growth without adverse effects. Accordingly, menthol was conjugated to BSA (MeB) to overcome the BBB-permeability issue. In this study, Abz-loaded MeB-silver nanoparticles (MBS-Abz) were developed by self-assembly of MeB, albendazole and nano silver for glioma targeting therapy. The nanoparticle entered the brain across the BBB and specifically accumulated in the glioma region. MeB delivered the nanoparticles across the brain capillary endothelial cell monolayer by promoting internalization and BBB disruption. MBS-Abz hindered ATP generation via the inhibition of glycolytic and mitochondrial pathways both in vitro and in vivo. The anti-glioma mechanisms of energy restriction were related to the cytotoxicity, proliferation inhibition, cell cycle arrest, and apoptosis induced by ATP exhaustion, and the effects of MBS-Abz were significantly better than those induced by monotherapy nanoparticles or unmodified nanoparticles. These results demonstrated that by combining the energy restriction effect of albendazole and nano silver, as well as the BBB penetration ability of menthol, MBS-Abz achieves superior anti-glioma efficacy and can be an effective strategy for glioma therapy.
研究领域:
药理学
·
神经科学
·
纳米技术
·
癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Tian-Tian Zhao,Tian-Jiao Zhou,Chen Zhang,Ying-Xuan Liu,Wen-Jia Wang,Chengjun Li,Lei Xing,Hu-Lin Jiang
Chemotherapy is the main treatment method for osteosarcoma in the clinic. However, drug resistance and its poor antimetastatic effects greatly limit its clinical application. In this work, dual-drug nanoparticles (NPs) containing albendazole (ABZ) and doxorubicin (DOX), named AD@PLGA–PEG NPs, were prepared to solve the problems of chemotherapeutic drug resistance and poor antimetastasis effects. Compared with free DOX, ABZ combined with DOX can increase intracellular reactive oxygen species (ROS) and induce more tumor cell apoptosis; therefore, AD@PLGA–PEG NPs produced more mitochondria-mediated oxidative stress and better apoptosis efficiency. Importantly, ABZ can also effectively inhibit the expression of hypoxia inducible factor-1α (HIF-1α) and then reduce the expression of its downstream vascular endothelial growth factor (VEGF); thus, the AD@PLGA–PEG NPs effectively inhibited tumor metastasis in vivo. Collectively, the dual-drug AD@PLGA–PEG NPs delivery system provided prominent antitumor and antimetastatic efficacy and might be a promising treatment for osteosarcoma.
研究领域:
肿瘤学
·
药学
·
纳米技术
·
癌症生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）