MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Menghua Liu,Shuangying Li,Zhi Li,Yanhui Li,Yue Zhang,Jingwen Niu,Linchun Nie,Chunmao Chen,Qingxiang Zhou
Amoxicillin is one of the most important semi-synthetic penicillin broad-spectrum β-lactam antibiotics and has achieved many applications. There are few reports on its application for synthesizing carbon dots (CDs). Present work explored the possibility of amoxicillin converted into CDs with sodium carbonate used as the co-reactant employing facile hydrothermal method. The obtained CDs exhibited good selectivity and sensitivity to benzidine and could be explored as an effective fluorescence probe for monitoring benzidine. The fluorescence intensity of CDs was quenched based on the principle of static quenching due to the formation of a non-fluorescent complex between CD and benzidine. Within the range of 0.2–2 μM and 2–16 μM, CDs possessed good linear characteristics, high sensitivity, and a low detection limit of 0.08 μM. The results demonstrated that the fluorescence probe possessed the merits of simplicity, rapidity, low cost, short analytical time, high selectivity and sensitivity, and good stability. According to the verification results from the real water samples, the fluorescence probe had high reliability and feasibility, and could be employed for monitoring benzidine in waters.
研究领域:
分析化学
·
环境监测
·
纳米材料
·
荧光传感
·
药物化学
✓ 已验证
DOI
高影响因子
MARINE POLLUTION BULLETIN
·
2019
·
IF 3.782
Qiuyu Li,Weihong Zhao,Hui Miao,Xiaotian Han
This work attempted to prolong the validity of the molybdate mixed solution and ascorbic acid solution used in the phosphomolybdenum blue spectrophotometric method by improving their preservation according to the influence factors. The results showed that the molybdate mixed solution can be directly preserved in darkness with validity over half a year. The ascorbic acid solution is influenced by light, temperature, pH, metal ions , oxygen, and bacteria. The validity of ascorbic acid is shortened as the temperature rises. Through keeping in darkness, adding complexing agents , adjusting pH, removing oxygen and sterilization, the validity of ascorbic acid solution was prolonged to over 2.7 times under 4 °C and over 5 times under 25 °C. At the same time, the hybrid solution of ascorbic acid solution and molybdate mixed solution should be preserved separately, otherwise the using effect is poor.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Sihua Qian,Li’na Qiao,Wenxiang Xu,Kai Jiang,Yuhui Wang,Hengwei Lin
Antibiotics play important roles in the treatment and prevention of bacterial infections. However, the over-use of antibiotics can lead to a lot of adverse side-effects like nerve damage, anaphylaxis and drug tolerance. Herein, we report an inner filter effect (IFE)-based near-infrared (NIR) fluorescent probe for highly sensitive and selective detection of tetracyclines (TCs) and quinolones (QNs), of which being the most frequently applied antibiotics. NIR emissive carbon-dots (NIR-CDs) that displayed broad excitation range, stable and strong emission were selected as label-free fluorophores. In the presence of TCs or QNs, efficient IFE from CDs to the two types of antibiotics occurred because of the good spectrum overlap between the ultraviolet absorption of TCs/QNs and the excitation of NIR-CDs. As a result, fluorescence emission of the NIR-CDs was obviously quenched, and this builds the foundation of TCs and QNs quantification. The probe provides linear detection ranges, i.e. 1–80 nM and 0.01–0.2 μM for oxytetracycline (OTC, an example for TCs) and norfloxacin (NOR, an example for QNs) with detection limits of 0.5 nM and 6.3 nM, respectively. To the best of our knowledge, this is the first report on NIR fluorescent probe for antibiotics assay. Moreover, the probe has been demonstrated to be very robust, and was successfully adopted for the determination of OTC and NOR in milk samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2018
·Vol. 6
·期 36
·9636-9641
·
IF 5.976
Zhi Shen,Cheng Zhang,Xinling Yu,Jian Li,Zhenyang Wang,Zhongping Zhang,Bianhua Liu
Carbon dots (CDs) prepared by the crosslinking and carbonization reaction between the carboxyl and amino groups of organic molecules have the characteristics of high fluorescence quantum yield and tunable emission colors, enabling their wide use in chemical sensing and bioimaging. However, straight-chain organic amines are mostly used in the preparation of carbon dots. Here, we chose cyclen that contained an azamacrocyclic ring and citric acid to synthesize bright blue-emitting fluorescent CDs by the microwave method. Under 750 W microwave irradiation, the condensation–crosslinking–carbonization process can be completed within 5 minutes with a quantum yield of up to 27.6%. Taking the advantage of using cyclen, the resulting CDs still have a ring-like structure on the surface, and thus can coordinate with Eu3+ ions to construct a ratiometric fluorescent probe for tetracycline detection. When tetracycline is present in the detection system, a CD–Eu3+–tetracycline ternary complex is formed and generates an intense red emission of Eu3+. Meanwhile, the blue emission of the CDs remains unchanged, realizing accurate tetracycline detection by recording the red to blue emission ratio. Besides, the output color changes from blue to red upon increasing the concentration of tetracycline. Therefore, a smartphone based tetracycline detection technique was successfully developed through the corresponding RGB value analysis.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-8
·
IF 5.705
Zhou Yunlei,Sui Chengji,Yin Huanshun,Wang Yue,Wang Minghui,Ai Shiyun
A method is described for photoelectrochemical determination of chloramphenicol (CLOA). It is based on the use of (a) aptamers protected with photoactive WS 2 nanosheets, and (b) DNase I-assisted target recycling. The DNA aptamer without label was employed for recognition of CLOA. In the absence of CLOA, the aptamer is adsorbed on the surface of WS 2 . This leads to a decrease of photocurrent due to the steric-hindrance effect of aptamer DNA. The adsorption of WS 2 also protects the aptamer from digestion by DNase. In the presence of CLOA, the aptamer will be desorbed from the WS 2 surface due to formation of an aptamer/CLOA conjugate. This results in an increased photocurrent due to a decreased amount of aptamer DNA on the electrode surface. The increase of photocurrent can be further improved by applying DNase triggered catalytic recycling of CLOA. Under optimal experimental conditions, the response is linear 10 pM – 10 nM CLOA concentration range, with a 3.6 pM lower detection limit (at 3σ). This method is acceptably selective, accurate and stable. It was applied to the determination of CLOA in spiked milk samples and gave satisfactory results.
A simple and sensitive photoelectrochemical apta-biosensor was fabricated for chloramphenicol detection. In this work, WS 2 nanosheets were employed as photoactive material, and DNase I catalytic chloramphenicol recycling strategy was adopted to amplify the detection signal.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Songlin Wang,Junfeng Wu,Xiuqing Lu,Wenxin Xu,Qing Gong,Jiaqi Ding,Bingsong Dan,Pengchao Xie
In this study, the removal of acetaminophen (ACT) in the Fe 2+ /persulfate system has been comprehensively evaluated. A kinetic model has been established based on the principal reactions. Good accordance between the experimental data and the predicted results under different operational conditions including molar ratio of Fe 2+ to persulfate, initial pH, initial ACT concentration, the presence of chloride, under natural water matrices and the mixture of ACT, amoxicillin and pyridine, evidences the validity of the proposed kinetic model. The rate constants for ACT reacting with SO 4 − and HO were determined as (1.80 ± 0.17) × 10 9 M −1 s −1 and (3.26 ± 0.41) × 10 9 M −1 s −1 , respectively. SO 4 − was evidenced to be the primary radical accounting for ACT removal through both kinetic model calculation and the scavenging results, using either methanol or tert -butyl alcohol. The optimum molar ratio of Fe 2+ to persulfate for ACT removal was determined to be 5:4. The removal rate of ACT was little affected by initial pH due to the rapid decrease of solution pH caused by the hydrolysis of iron ions. Cl − has a dual effect on the removal of ACT, and Cl 2 − rather than SO 4 − is proposed as the dominant radical for ACT removal at 10.0 mM Cl − . Possible degradation pathways are also proposed based on the detected intermediates.
✓ 已验证
产品关联
DOI
高影响因子
Open Chemistry
·
2018
·Vol. 16
·期 1
·949-955
·
IF 1.425
Lvshan Zhou,Xiaogang Guo,Chuan Lai,Wei Wang
The electro-photocatalytic degradation of amoxicillin in aqueous solution was investigated using single factor test by the potassium permanganate method for measuring the values of chemical oxygen demand (COD Mn ). Batch experiments were carried out successfully under different conditions, including initial amoxicillin concentration, calcium titanate dosage, pH, UV irradiation time, electrolyte and temperature. The experimental results show that there is a great difference between electro-photocatalytic and photocatalitic degradation. The maximum electro-photocatalytic degradation efficiency can increase to 79% under the experimental conditions of 200 mL amoxicillin solution (100 mg L -1 ) with 0.5 g calcium titanate by pH=3 for 120 min irradiation and 0.058 g sodium chloride as electrolyte at 318.5K. In addition, the reaction rate constant of 0.00848~0.01349 min -1 , activation energy of 9.8934 kJ mol -1 and the pre-exponential factor of 0.5728 were obtained based on kinetics studies, indicating that the electro-photocatalytic reaction approximately followed the first-order kinetics model.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Yao Yao,Chengmei Jiang,Jianfeng Ping
Flexible sensing devices have drawn tremendous attention in the past decades due to their potential applications in future hand-held, potable consumer, and wearable electronics . Here, we firstly developed an ultrasensitive wireless potentiometric aptasensor based on flexible freestanding graphene paper for kanamycin detection. Flexible graphene paper made from a simple vacuum filtration method was used as a biocompatible platform for effective immobilization of aptamer. A nuclease-assisted amplification strategy was introduced into this potentiometric biosensing system in order to significantly improve the detection sensitivity through a classic catalytic recycling reaction of target induced by the nuclease (DNase I). As expected, an ultra-low detection limit of 30.0 fg/mL for kanamycin was achieved. Furthermore, the developed potentiometric enzymatic aptasensor exhibits high selectivity , favorable flexibility, excellent stability and reproducibility, which holds great promising for its routine sensing application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Chengji Sui,Yunlei Zhou,Mengying Wang,Huanshun Yin,Po Wang,Shiyun Ai
Graphite-like carbon nitride (g-C 3 N 4 ) nanosheet is a new type of photoactive semiconducting material with promising application in photoelectrochemical (PEC) biosensor. In the present work, a simple and selective PEC biosensor was fabricated using g-C 3 N 4 nanosheets as photoactive material coupled with capture-release strategy. DNA aptamer labeled with ferrocene (DNA-Fc) was served as both recognition reagent of antibiotic chloramphenicol and electron donor of PEC biosensor. Based on the decreased affinity of aptamer to g-C 3 N 4 nanosheets causing by the formation of aptamer-chloramphenicol complex, DNA-Fc could be released from g-C 3 N 4 nanosheets modified ITO surface, resulting a decreased PEC response. The developed method showed a wide linear range from 1 pM to 100 nM and a low detection limit of 0.22 pM (3σ). The fabricated PEC biosensor also illustrated good detection specificity and can be applied to detect chloramphenicol residue in water samples with recoveries ranging from 94.5% to 107.3%.
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 39
·16381-16388
·
IF 7.394
Yanni Chen,Yongwei Wang,Liqiang Liu,Xiaoling Wu,Liguang Xu,Hua Kuang,Aike Li,Chuanlai Xu
A novel gold immunochromatographic assay (GICA) based on anti-β-lactam receptors was innovatively developed that successfully allowed rapid and simultaneous detection of fifteen β-lactams in milk samples in 5–10 minutes. By replacing the antibodies used in traditional GICA with anti-β-lactam receptors, the difficulty in producing broad specific antibodies against β-lactams was overcome. Conjugates of ampicillin with BSA and goat anti-mouse immunoglobulin (IgG) were immobilized onto the test and control lines on the nitrocellulose membrane, respectively. Since goat anti-mouse IgG does not combine with receptors, negative serum from mice labelled with gold nanoparticles (GNP) was mixed with GNP-labelled receptors. Results were obtained within 20 min using a paper-based sensor. The utility of the assay was confirmed by the analysis of milk samples. The limits of detection (LOD) for amoxicillin, ampicillin, penicillin G, penicillin V, cloxacillin, dicloxacillin, nafcillin, oxacillin, cefaclor, ceftezole, cefotaxime, ceftiofur, cefoperazone, cefathiamidine, and cefepime were 0.25, 0.5, 0.5, 0.5, 1, 5, 5, 10, 25, 10, 100, 10, 5, 5, and 2 ng mL−1, respectively, which satisfies the maximum residue limits (MRL) set by the European Union (EU). In conclusion, our newly developed GICA-based anti-β-lactam receptor assay provides a rapid and effective method for one-site detection of multiple β-lactams in milk samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Xiuzhong Wang,Shanshan Dong,Panpan Gai,Rui Duan,Feng Li
The ubiquitous presence of antibiotic residues in foodstuff have serious health consequences for consumers from allergic reactions to the evolution of antibiotic-resistant bacteria. To address this problem, a novel homogeneous electrochemical aptasensor with high sensitivity and specificity is designed for antibiotic residues detection based on target-induced and T7 exonuclease-assisted dual recycling signal amplification strategy. It was realized by the remarkable diffusivity difference between hairpin probe and the mononucleotides towards the negatively charged indium tin oxide electrode. For the proof-of-concept experiment, ampicillin , was employed as a model analyte to examine the desirable properties of this assay. A low detection limit of 4.0 pM toward ampicillin with an excellent selectivity could be achieved, which has been successfully applied to assay antibiotic in milk. What's more, compared with the immobilization-based electrochemical means, the proposed sensing system avoids the tedious and time-consuming steps of electrode modification, making the experimental processes much simpler and more convenient. With the advantages of high sensitivity, excellent selectivity and simple operation, it is believed that this strategy possesses great potential for the simple, easy and convenient detection of antibiotic residues in food safety field.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Zewei Luo,Yimin Wang,Xiaoyong Lu,Junman Chen,Fujing Wei,Zhijun Huang,Chen Zhou,Yixiang Duan
Antibiotic abuse has been bringing serious pollution in water, which is closely related to human health. It is desirable to develop a new strategy for antibiotic detection. To address this problem, a sensitive fluorescent aptasensor for antibiotic detection was developed by utilizing gold nanoparticles modified magnetic bead composites (AuNPs/MBs) and nicking enzyme . AuNPs/MBs were synthesized with the help of polyethylenimine (PEI). The prepared AuNPs/MBs acted as dual-functional scaffolds that owned excellent magnetic separation capacity and strong covalent bio-conjugation. The non-specifically absorbed aptamers in AuNPs/MBs were less than that in MBs. Hence, the fluorescent aptasensor based on AuNPs/MBs show a better signal to background ratio than that based on carboxyl modified magnetic beads (MBs). In this work, ampicillin was employed as a model analyte . In the presence of ampicillin, the specific binding between ampicillin and aptamer induced structure-switching that led to the release of partial complementary DNA (cDNA) of aptamer. Then, the released cDNA initiated the cycle of nicking enzyme assisted signal amplification (NEASA). Therefore, a large amount of taqman probes were cleaved and fluorescence signal was amplified. The prepared fluorescent aptasensor bring sensitive detection in range of 0.1–100 ng mL −1 with the limit of detection of 0.07 ng mL −1 . Furthermore, this aptasensor was also successfully applied in real sample detection with acceptable accuracy. The fluorescent aptasensor provides a promising method for efficient, rapid and sensitive antibiotic detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）