FOOD CHEMISTRY
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2022
·
IF 9.231
Shuai Zhang,Qin Ma,Lihong Dong,Xuchao Jia,Lei Liu,Fei Huang,Guang Liu,Zhida Sun,Jianwei Chi,Mingwei Zhang,Ruifen Zhang
Phytochemicals are unevenly distributed in grain kernels and concentrated in bran fractions. However, their specific distribution in the grain bran, especially colored grains, is not clarified. This study divided rice bran from black rice into five fractions by stepwise milling to obtain BF1(outermost layer) to BF5 (the innermost layer). Each fraction accounted for approximately 2% of the whole kernel. The total content of phenolics (TPC), flavonoids (TFC), and anthocyanins (TAC) of five fractions significantly decreased from BF1 to BF5. The TPC, TFC and TAC of BF1 contribute 25.7%, 28.2%, 28.4% to the total of five fractions, respectively. HPLC analysis showed that the contents of most anthocyanin and phenolic acids compounds decreased from BF1 to BF5. Together with α -glucosidase and α -amylase inhibitory activities of BF1, the antioxidant activity was higher than those of other fractions. These results can guide the moderate processing of black rice and the utilization of its bran.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 9
·5909-5916
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IF 3.612
Guanghe Zhao,Ruifen Zhang,Lihong Dong,Lei Liu,Fei Huang,Xuchao Jia,Mei Deng,Jianwei Chi,Yongxuan Ma,Yanxia Chen,Qin Ma,Mingwei Zhang
Summary Phenolics in whole grains naturally occur in free and bound forms, and are dominated by the latter. Previous study indicated that bound phenolics played an indispensable role in the health benefits of whole grain dietary fibre. However, existing extraction methods can only partly release bound phenolics, which results in an underestimation of their contents and activity. The content, composition and antioxidant activity of bound phenolics released from rice bran dietary fibre (RBDF) by different chemical hydrolysis methods were analysed in this study to explore a method releasing much more bound phenolics. The results showed that alkaline hydrolysis could release 1.54 times more phenolics than acid hydrolysis. The combination of 2 hydrolysis methods, especially alkaline-acid hydrolysis (the residue after alkaline hydrolysis was then hydrolysed with acid), could more effectively release bound phenolics from RBDF. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) antioxidant activity of the RBDF hydrolysates from different hydrolysis methods were generally consistent with their phenolic contents. However, the alkaline hydrolysate had 7.62% less phenolics, but showed 53.03% higher CAA activity than the acid-alkaline hydrolysate. The RBDF residues after different chemical hydrolysis, furthermore, exhibited considerable DPPH and FRAP activity by QUENCHER method, indicating their possible local antioxidant activity in the colonic microenviroment.
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高影响因子
AMB Express
·
2021
·Vol. 11
·期 1
·1-10
·
IF 3.298
Yao Jian,Gui Lun,Yin Shaocheng
A novel esterase gene was isolated from a soil metagenomic library. The gene encoded a protein of 520 amino acids which contained a 21 aa signal peptide. Primary structure analysis of the protein sequence revealed that it contained a conserved active site motif (SxSxG) and a structural motif (CS-D-HC). Then the esterase gene was cloned and expressed in Escherichia coli BL21(DE3). SDS-PAGE analysis of the purified esterase showed that it was expressed in a highly soluble form and its molecular mass was estimated to be 55 kDa. Characterization of the esterase revealed that it exhibited high activity toward p-nitrophenyl esters with short acyl chains and especially p-nitrophenyl acetate, suggesting that it was a typical carboxylesterase rather than a lipase. With p-nitrophenyl acetate as substrate, the enzyme showed its optimal activity at pH 7.0 and 30 °C, and it was stable at a broad pH range from 4.5 to 10.0 and temperature not higher than 50 °C. Furthermore, the enzyme showed different substrate specificity from known esterase, it was not only hydrolyzing against p-nitrophenyl esters, but also hydrolyzing all hydroxybenzoic esters and hydroxycinnamic ester assayed. As it was an enzyme active on a broad range of phenolic esters, simultaneously possessing feruloyl esterase, chlorogenate esterase and tannase activities, it could serve as a valuable candidate for applications in biotechnology.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
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IF 5.162
Weiyue Liang,Tianzhen Xiong,Xiaomei Wang,Huaxiang Deng,Yajun Bai,Tai-Ping Fan,Xiaohui Zheng,Yujie Cai
A novel feruloyl esterase (BpFae12) with rosmarinic acid (RA) hydrolysis activity was isolated from Bacillus pumilus W3 and expressed in Escherichia coli BL21 (DE3). With RA as a substrate, the optimal pH and temperature of BpFae12 were pH 8.0 and 50 °C, respectively. The specific enzyme activity was 12.8 U·mg −1 . BpFae12 showed the highest activity and substrate affinity toward RA ( V max of 13.13 U·mg −1 , K m of 0.41 mM). Moreover, it also presented strong hydrolysis performance against chlorogenic acid (190.17 U·mg −1 ). RA was effectively Hydrolyzed into more bioactive caffeic acid and 3,4-dihydroxyphenyllactic acid by BpFae12, which have potential applications in the food industry.
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yueqin Li,Yunzhen Zhang,Lezhen Dong,Ying Li,Yahui Liu,Yang Liu,Lingyi Liu,Lianliang Liu
In this study, strains producing feruloyl esterase were screened by Oxford Cup clear zones method and by evaluating the ability to decompose hydroxycinnamoyl esters. The strain was identified by 16S rDNA molecular biology. The contents of dietary fiber, reducing sugar, water-extractable arabinoxylans, phytic acid, total phenolics, total flavonoid, phenolic compounds composition, microstructure and antioxidant activity in bran before and after fermentation were studied. Eight strains producing feruloyl esterase were screened, among which strain P 1 had the strongest ability to decompose hydroxycinnamoyl esters. The strain was identified and named L. fermentum NB02. Compared with unfermented bran, fermented bran exhibited higher contents of soluble dietary fiber, reducing sugar, water-extractable arabinoxylans, total phenolics, total flavonoid, and lower insoluble dietary fiber and phytic acid content. The dense surface structure of bran was destroyed, forming a porous structure. The release of phenolic compounds increased significantly. L. fermentum NB02 fermentation improved the antioxidant capacity of bran.
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NEW JOURNAL OF CHEMISTRY
·
2023
·
IF 3.3
Wenbin Jin,Kaipeng Wang,Xueyang Xu,Yiling Xu,Fengwei Li,qingzhi ji,Xiaodong Chen,Xiaohong Yu
Ferulic acid is a natural phenolic chemical which widely present in plants and widely used in food and cosmetic industries. It is used as the cross-linking agent for the preparation of edible films, and as skin protection agents. Enzymatic hydrolysis of lignocellulose to produce ferulic acid has been studied for many years and is rarely used in the industry due to easy deactivation of enzymes. Deep eutectic solvent (DES) is exploited as a novel co-solvent for improving the activity and stability of enzyme. A mixed enzyme system composed of feruloyl esterase and xylanase was constructed for catalytic degradation of de-starched wheat bran (DSWB) to produce ferulic acid. When the ratio of feruloyl esterase to xylanase reached 1:1, the release of ferulic acid reached the maximum. Four DES was synthesized and evaluated the effects of compositions and concentrations on the release of ferulic acid from DSWB. The mixed enzymatic system showed the best performance in the ChCl-Gly DES aqueous solution (volume ratio of 1:2). Through the determination of the enzymatic properties and catalytic conditions, the mixed enzymatic system was applied to the catalytic reaction. Ferulic acid yield 4.2 mg/g DSWB was obtained under the optimum conditions. The results suggested that the efficient production of ferulic acid from lignocellulosic biomass can be realized through the synergistic effect of feruloyl esterase and xylanase in DES aqueous solution.
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Food Bioscience
·
2023
·
IF 5.2
Guanghe Zhao,Ruifen Zhang,Lihong Dong,Mei Deng,Yanxia Chen,Mingwei Zhang
Bound phenolics are the main phytochemicals and important contributors to the health benefits of whole grains . The commonly used chemical methods for bound phenolic release are not appropriate for processing of food materials. The effects of cellulase and feruloyl esterase on releasing bound phenolics from rice bran dietary fibre (RBDF) and the auxiliary effects of autoclaving pretreatment were investigated in the present study to find a method to effectively increase bioaccessible phenolics in RBDF. The results showed that cellulase (CT) and feruloyl esterase (FT) alone and combined application (CFT) released limited bound phenolics. Autoclaving pretreatment could enhance the release of bound phenolics by enzymatic treatments significantly with 1.74, 5.56 and 1.76 folds increases when compared with the above mentioned enzymolysis, respectively. Moreover, the total quantity of phenolic compounds released from RBDF via combined enzymolysis of cellulase and feruloyl esterase with autoclaving pretreatment (A-CFT) were comparable with that via alkaline hydrolysis previously reported. The predominant phenolic compounds released by A-CFT from RBDF were vanillin and ferulic acid , while ferulic and p -couramic acids accounted for 99.30% of the sum of phenolics released by alkaline hydrolysis. The DPPH scavenging, FRAP and ORAC antioxidant activities of the enzymatic hydrolysates were positive correlated with their total phenolic contents. The phenolics released by A-CFT showed lower antioxidant activity than those by alkaline hydrolysis, which may ascribe to the difference in phenolic profiles between methods. This study provides a new approach for processing whole grain and the by-products for functional foods materials with higher phenolic bioaccessibility.
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高影响因子
MOLECULES
·
2018
·Vol. 23
·期 1
·202
·
IF 3.098
Guanghe Zhao,Ruifen Zhang,Lihong Dong,Fei Huang,Lei Liu,Yuanyuan Deng,Yongxuan Ma,Yan Zhang,Zhencheng Wei,Juan Xiao,Mingwei Zhang
The composition, in vitro bioaccessibility and antioxidant activities of the phenolic compounds in defatted rice bran (DRB) and its soluble and insoluble dietary fibres were systematically evaluated in this study. The total phenolic content of insoluble dietary fibre from DRB (IDFDRB) was much higher than that of the soluble dietary fibre from DRB (SDFDRB) but was 10% lower than that of DRB. Bound phenolics accounted for more than 90% of the total phenolics in IDFDRB, whereas they accounted for 34.2% and 40.5% of the total phenolics in DRB and SDFDRB, respectively. Additionally, the phenolic profiles and antioxidant activities were significantly different in DRB, SDFDRB and IDFDRB. The phenolic compounds in IDFDRB were much less bioaccessibility than those in DRB and SDFDRB due to the higher proportion of bound phenolics in IDFDRB. Considering that bound phenolics could be released from food matrices by bacterial enzymes in the large intestine and go on to exert significant beneficial health effects in vivo, further studies on IDFDRB are needed to investigate the release of the phenolics from IDFDRB via gut microbiota and the related health benefits.Keywords:rice bran;dietary fibre;phenolic compounds;antioxidant activity;bioaccessibility
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Zan Long,Bingni Jia,Bingqian Jing,Xiaofeng Liu,Ke Tian,Peng Zhang,Bo Feng,Taiping Qing
The new detection technology represented by fluorescent probe is an effective supplement to the traditional instrument analysis of environmental pollutants. However, background interference is an inevitable obstacle in the fluorescent analysis of complex samples. Dissolved organic matter (DOM) in water is widespread and significantly affects the performance of fluorescent probes in pollutants detection. In this work, the impact of DOM on the performance of fluorescent probes were investigated under different conditions. Firstly, three-dimensional fluorescence spectroscopy of local lake was performed to identify the composition of organic matter in water. The types of chromophoric DOM in local lake mainly include humic acids and tryptophan, and its concentrations varied over time and across different regions. Then, three fluorescent probes with different fluorescence emission were selected to investigate the interaction between DOM (humic acids, tryptophan, and fulvic acid as the interfering substances) and fluorescent groups. The experimental results demonstrated that humic acid significantly reduced the signal intensity of fluorescent probes through mechanisms such as inner filter effects and fluorescence resonance energy transfer. In contrast, tryptophan and fulvic acid had relatively minor impacts. More importantly, the altered pH and ions of the environmental water did not significantly alter the interference of DOM on fluorescent probe. To further verify the influence of chromophoric DOM on fluorescent probes in real water, the water treatment under UV irradiation with H 2 O 2 was used for the preparation of simulated water samples. The influence of DOM on fluorescent probes in real water samples was also similar to that in buffer. These results suggested that the chromophoric DOM can effectively affect the spectral properties of different fluorescent probes, and greatly interfere with the sensitivity and accuracy of fluorescence detection. This work help to understand the interference mechanisms of DOM in water, and are significant for improving the accuracy of fluorescent probes in water quality monitoring applications.
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JOURNAL OF CATALYSIS
·
2024
·
IF 6.5
Yun Tian,Mengqiao Gao,Zhiyang Tang,Fukun Li,Qiang Zeng,Jinxing Long,Xuehui Li
Conversion of lignin to valuable chemicals without external hydrogen presents a significative but challengeable endeavor. Herein, an efficient approach has been proposed for the lignin hydrogenolysis with in-situ hydrogen in the presence of Ni nanoparticles nested in layered double oxides (LDOs). 84.4 % conversion of lignin was achieved over 30Ni-Mg 3 Al-LDOs, affording a 21.3 % yield of monomers, and 38.0 % of which was identified as 4-ethylphenol. Catalyst characterizations revealed that this excellent performance of lignin conversion should be ascribed to the highly dispersed Ni nanoparticles, strong interfacial interactions between Ni and LDOs, and suitable basicity of catalysts. Furthermore, the roles of the solvent (1,4-dioxane aqueous solution) and the lignin methoxyl groups as hydrogen resources were distinguished, and the former acted as an initial hydrogen to promote lignin depolymerization, and the latter was responsible for the high yield of 4-ethylphenol. In addition, a plausible reaction pathway for lignin hydrogenolysis has been proposed.
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Heliyon
·
2024
·
IF 3.4
Ying Yang,Shuqin Wang,Xingyan Liu,Wenbin Zhang,Wenhua Tong,Huibo Luo,Liming Zhao
Ferulic acid (FA) and ferulic acid methyl ester (FAM) are important phenolic compounds in Baijiu. In this study, the interaction of FA and FAM with human serum albumin (HSA) and lysozyme (LZM) was investigated using multispectral methods and molecular dynamics simulation. FA and FAM could interact with HSA and LZM, changing the conformation and hydrophilicity of the protein. The quenching mechanisms of FA-HSA, FA-LZM, FAM-HSA, and FAM-LZM were all static-quenching. In the FA-HSA, FAM-HSA, and FA-LZM systems, the interaction forces were mainly hydrophobic interactions and hydrogen bonding. In the FAM-LZM system, the interaction forces were mainly hydrophobic interactions, hydrogen bonding, and van der Waals force. Common metal ions such as K + , Ca 2+ , Cu 2+ , Mg 2+ , and Mn 2+ could affect the binding ability of FA and FAM to HSA and LZM. Moreover, FA and FAM could increase the stability of HSA and LZM, and the protein bound to FA/FAM was more stable than the free protein. FA and FAM had varying degrees of impact on the physiological activities of HSA and LZM. This study provides relevant information on the interactions and metabolic mechanisms of FA and its derivatives with endogenous proteins.
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高影响因子
危险品化学品经营许可证（带存储）