RSC Advances
·
2019
·Vol. 9
·期 29
·16701-16712
·
IF 3.049
Xiaofeng Li,Maohua Ma,Xuan Xin,Yuqian Tang,Guanglei Zhao,Xinglong Xiao
Gastrodin, a bioactive compound extracted from the plant source of Gastrodia elata Blume, has a wide range of therapeutic effects on central nervous system (CNS) diseases, but suffers from poor brain permeability and short half-life in plasma. In this study, fatty acid esters of gastrodin were successfully synthesized by a whole cell-based biocatalytic method. Aspergillus oryzae cells showed different catalytic activities in the organic solvent systems tested. Tetrahydrofuran was confirmed as the most suitable pure organic solvent, with the highest substrate conversion of 98.0%. Addition of ionic liquids (ILs) into pyridine dramatically accelerated the reaction with conversion increased from 5.9% to 84.2%, and also changed the selectivity of the cells, mainly due to the use of IL-containing systems altering cell permeability and contact of the enzymes with solvent molecules possessing different polarities. The ester products were characterized by 13C-NMR and ESI-MS as gastrodin monoester and diester.
研究领域:
药理学
·
生物催化
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2015
·
IF 1.884
Fuming He,Song Zhang,Xinli Liu
Feruloyl esterase plays an indispensable role in hydrolyzing plant cell walls, and ferulic acid will be released as by-product, which has potential applications in food and medicine industry. This study presents immobilization of partially purified feruloyl esterases from the fermentation liquor of recombinant Pichia on magnetic Fe 3 O 4 nanoparticles. Furthermore, the optimal conditions for the immobilization and some characteristics of immobilized enzyme were studied. The optimal immobilization conditions observed were enzyme 0.2 mg (1 mg/mL, 0.2 mL), magnetic Fe 3 O 4 nanoparticles 4 mg, pH 6.0, immobilization time 3 h. The results showed that the optimal reaction temperature of immobilized enzyme was increased from 45°C to 55°C. Thermal stability of the immobilized enzyme also had an improvement, the residual activity retained 80% and 69% after 120 h at 40°C and 50°C, respectively, while free enzymes only showed 45% and 40% remnant activity at the same condition. The immobilized enzyme also exhibited good operational stability and 52.4% of its initial activity was observed during the fifth cycle. In terms of the thermal and operational stability, the immobilized enzyme could be better used in many more applications than the free enzymes. At last the destarched wheat bran was taken as substrate for immobilized and free feruloyl esterases to produce ferulic acid, the maximum ferulic acid yield was 11.2% and 12.3%, respectively, indicating a great potential in industrial application.
研究领域:
生物技术
·
酶工程
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2016
·Vol. 38
·期 6
·1009-1013
·
IF 1.639
Kang Le,Bai Yajun,Cai Yujie,Zheng Xiaohui
Objectives To characterize a novel feruloyl esterase from Escherichia coli BL21 DE3. Results The gene encoding BioH was cloned and overexpressed in E. coli . The protein was purified and its catalytic activity was assessed. BioH exhibited feruloyl esterase activity toward a broad range of substrates, and the corresponding kinetic constants for the methyl ferulate, ethyl ferulate, and methyl p -coumarate substrates were: K
m
values of 0.48, 6.3, and 1.9 mM, respectively, and k
cat
/ K
m
values of 9.3, 3.8, and 3.8 mM −1 s −1 , respectively. Conclusions Feruloyl esterase from E. coli was expressed for the first time. BioH was confirmed to be a feruloyl esterase.
研究领域:
生物化学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Oleo Science
·
2021
·
IF 1.601
Hongli Yang,Xu Han,Ahmed S. M. Saleh,Chen Shao,Yumin Duan,Zhi-gang Xiao
In this study, Novozym 435-catalyzed interesterification of ethyl ferulate (EF) with phosphatidylcholine (PC) in a two-phase system consisting of an ionic liquid (IL) and toluene was optimized to prepare feruloylated lysophospholipids (FLPs). Optimum conditions for the interesterification process were found to be [Bmim][Tf2N]/toluene ratio of 1:1 (v/v), solvent volume of 4 mL, molecular sieves (4 Å) concentration of 80 mg/mL, reaction temperature of 55°C, substrate molar ratio of 5:1 (PC/EF), Novozym 435 concentration of 50 mg/mL. Under these conditions, two FLPs products (1-FLP and 2-FLP) with total conversion rate of 50.79% were obtained. Because the formation of 1-FLP was significantly higher than 2-FLP, 1-FLP was purified and characterized by LC-MS and NMR. In addition, 1-FLP showed DPPH scavenging activity comparable with those of EF and BHT. Therefore, this study provides a good method for transformation of ferulic acid to improve its solubility and promote its application as functional ingredient in the food and pharmaceutical industries.
研究领域:
食品科学
·
生物化学
·
制药工程
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yueqin Li,Yunzhen Zhang,Lezhen Dong,Ying Li,Yahui Liu,Yang Liu,Lingyi Liu,Lianliang Liu
In this study, strains producing feruloyl esterase were screened by Oxford Cup clear zones method and by evaluating the ability to decompose hydroxycinnamoyl esters. The strain was identified by 16S rDNA molecular biology. The contents of dietary fiber, reducing sugar, water-extractable arabinoxylans, phytic acid, total phenolics, total flavonoid, phenolic compounds composition, microstructure and antioxidant activity in bran before and after fermentation were studied. Eight strains producing feruloyl esterase were screened, among which strain P 1 had the strongest ability to decompose hydroxycinnamoyl esters. The strain was identified and named L. fermentum NB02. Compared with unfermented bran, fermented bran exhibited higher contents of soluble dietary fiber, reducing sugar, water-extractable arabinoxylans, total phenolics, total flavonoid, and lower insoluble dietary fiber and phytic acid content. The dense surface structure of bran was destroyed, forming a porous structure. The release of phenolic compounds increased significantly. L. fermentum NB02 fermentation improved the antioxidant capacity of bran.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Zhang Kai,Xin Jiaying,Lu Xuechun
This study established a Candida rugosa lipase (CRL) system to catalyze triolein and ethyl ferulate interesterification. The products were identified, and the binding mode between the substrates and CRL was predicted through molecular docking. Three methods for preparing CRL-AuNPs were proposed and characterized. It was found that the addition of 40 mL of 15 nm gold nanoparticles increased the CRL activity from 3.05 U/mg to 4.75 U/mg, but the hybridization efficiency was only 32.7 %. By using 4 mL of 0.1 mg/mL chloroauric acid, the hybridization efficiency was improved to 50.7 %, but the enzyme activity was sharply decreased. However, when the molar ratio of Mb to HAuCl 4 was 0.2, the hybridization efficiency increased to 71.8 %, and the CRL activity was also enhanced to 5.98 U/mg. Under optimal conditions, the enzyme activity of CRL-AuNPs③ was maintained at 95 % after 6 repetitions and 85.6 % after 30 days at room temperature.
研究领域:
生物化学
·
酶工程
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Mengxing Zhao,Xingye An,Zhongqiu Fan,Shuangxi Nie,Zhengbai Cheng,Haibing Cao,Xiaohong Zhang,Md Manik Mian,Hongbin Liu,Liqin Liu
Tobacco based cellulose nanofiber (TCNF) is a novel nanocellulose that has recently been used to replace undesirable wood pulp fibers in the preparation of reconstructed tobacco sheets (RTS). However, given the strict requirements for controlling toxic chemical content in tobacco products, there is a global interest in developing a green, efficient, and toxic-chemical free approach to isolate TCNF from tobacco stem as a bioresource. In this study, we propose a creative and environmentally friendly method to efficiently and safely isolate TCNF from tobacco stem pulp, which involves integrated biological pretreatment followed by a facile mechanical defibrillation process. Feruloyl esterase is used to pretreat the stem pulp by disrupting the ether and ester bonds between lignin and polysaccharide carbohydrates within the fiber wall, which effectively facilitates cellulase hydrolysis and swelling of the stem pulp fiber, as well as the following mechanical shearing treatment for TCNF isolation. The results demonstrate that TCNF obtained by the comprehensive feruloyl esterase/cellulase/mechanical process exhibit uniform and well-dispersed nanofiber morphology, higher crystallinity , and stronger mechanical properties than those of the control. The addition of 0.5 % TCNF can replace wood pulp by 18 wt% ~ 25 wt% in the production of RTS samples while maintaining their reasonable strength properties.
研究领域:
生物技术
·
材料科学
·
烟草学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Shujun Liu,Lubna Soomro,Xue Wei,Xufeng Yuan,Tianyi Gu,Zhen Li,Yulu Wang,Yuming Bao,Fengzhong Wang,Boting Wen,Fengjiao Xin
Producing ferulic acid (FA) from the natural substrate with feruloyl esterase is promising in industries, screening and engineering new enzymes with high efficiency to increase the FA yield is of great concern. Here, the feruloyl esterase of Lactobacillus acidophilus (FAELac) was heterologous expressed and the FAELac with different oligomerization states was separated. Interestingly, the activity of dimer was 37-fold higher than high-polymer. To further enhance the efficiency of FAELac, eight mutants were generated based on the simulated structure, of which Q198A, Q134T enhanced the catalytic efficiency by 5.4- and 4.3-fold in comparison with the wild type. Moreover, higher yields of FA (2.21, 6.60, and 1.67 mg/g substrate, respectively) were released by the mutants from de-starched wheat bran, insoluble wheat arabinoxylan, and steam-exploded corn stover. These results indicated that improving the purification process, engineering new FAELac and substrates bias studies hold great potential for increasing FA production yield.
研究领域:
生物技术
·
酶学
·
生物化学
·
工业生物技术
✓ 已验证
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2022
·
IF 6.751
Hui Kong,Xincheng Fu,Xia Chang,Zemin Ding,Yang Yu,Haishan Xu,Rongrong Wang,Yang Shan,Shenghua Ding
Alternaria alternata is the principal pathogenic fungus causing postharvest black spot disease of Korla pear, leading to enormous economic losses. This study aimed to explore the antifungal activity of ferulic acid (FA) and its two simple esterified derivatives, methyl ferulate (MF) and ethyl ferulate (EF), against A. alternata in vitro and in vivo , and to reveal the underlying inhibitory mechanisms. The results showed that MF and EF exhibited stronger inhibitory effects than FA on mycelial growth, spore germination and germ tube elongation of A. alternata in vitro , and on reducing the development of black spot disease in Korla pear fruit. Scanning electron microscopy images indicated that the surface morphology of A. alternata hyphae treated with FA, MF, and EF presented irreversible deformation, and transmission electron microscopy observation further demonstrated that MF and EF caused more serious damage to the cell membranes, cytoplasm, and organelles of A. alternata than FA, which impeded the normal growth of A. alternata . Notably, EF was slightly better than MF in inhibiting spore germination and damaging cell ultrastructure of A. alternata . In addition, propidium iodide staining suggested that FA, MF, and EF treatments damaged the plasma membrane integrity of A. alternata spores. At the same conditions, MF and EF treatments induced more leakage of intercellular electrolytes, nucleic acids and proteins of A. alternata than FA, indicating the increase of cell membrane permeability . Taken together, all these results evidenced that MF and EF exerted stronger antifungal activity than FA against A. alternata in vitro and in vivo through membrane damage mechanism, therefore they can be used as promising green substitutes to control postharvest black spot disease on Korla pear fruit.
研究领域:
植物病理学
·
植物化学
·
食品科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hongling Wang,Xianghui Qi,Song Gao,Guoshi Kan,Lkhagvasuren Damdindorj,Yingfeng An,Fuping Lu
Herein, a novel multifunctional enzyme β-glucosidase/xylanase/feruloyl esterase (GXF) was constructed by fusion of β-glucosidase and bifunctional xylanase/feruloyl esterase. The activities of β-glucosidase, xylanase, feruloyl esterase and acetyl xylan esterase displayed by GXF were 67.18 %, 49.54 %, 38.92 % and 23.54 %, respectively, higher than that of the corresponding single functional enzymes. Moreover, the GXF performed better in enhancing aroma and quality of Longjing tea than the single functional enzymes and their mixtures. After treatment with GXF , the grassy and floral odors of tea infusion were significantly improved. Moreover, GXF treatment could improve concentrations of flavonoid aglycones of myricetin, kaempferol and quercetin by 68.1-, 81.42- and 77.39-fold, respectively. In addition, GXF could accelerate the release of reducing sugars, ferulic acid and xylo -oligosaccharides by 9.48-, 8.25- and 4.11-fold, respectively. This multifunctional enzyme may have potential applications in other fields such as food production and biomass degradation.
研究领域:
酶学
·
食品科学
·
茶叶技术
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2020
·
IF 4.236
Chen Miao,Liu Shujun,Imam Khandaker Md. Sharif Uddin,Sun Lichao,Wang Yulu,Gu Tianyi,Wen Boting,Xin Fengjiao
Wheat bran is a cereal rich in dietary fibers that have high levels of ferulic acid, which has prebiotic effects on the intestinal microbiota and the host. Herein we explored the effect of xylooligosaccharide, xylan, and whole wheat bran on the human gut bacteria and screened for potential ferulic acid esterase genes. Using in vitro fermentation, we analyzed the air pressure, pH-value, and short-chain fatty acid levels. We also performed 16S rRNA gene and metagenomic sequencing. A Venn diagram analysis revealed that 80% of the core operational taxonomic units (OTUs) were shared among the samples, and most of the xylooligosaccharide treatment core OTUs (319/333 OTUs) were shared with the other two treatments’ core OTUs. A significant difference analysis revealed that the relative abundance of Dorea, Bilophila, and Sulfurovum in wheat bran treatment was higher than that in xylan and xylooligosaccharide treatments. The clusters of orthologous groups of proteins functional composition of all samples was similar to the microbiota composition of the control. Using metagenomic sequencing, we revealed seven genes containing the conserved residues, Gly-X-Ser-X-Gly, and the catalytic triad, Ser-His-Asp, which are thus potential ferulic acid esterase genes. All the results indicate that xylan and/or xylooligosaccharide, the main dietary fibers in wheat bran, plays a major role in in vitro fermentation by the human gut microbiota.
研究领域:
微生物学
·
营养科学
·
胃肠病学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
TOXICON
·
2024
·
IF 2.6
Xu He,Xiuhong Peng,Suyu Zhang,Tianfeng Yang,Jian Huo,Yanmin Zhang
Amphotericin B (AmB) induced liver and kidney injury is often responsible for hepatic and renal dysfunction. Therefore, the protection strategy on liver and renal functions in patients treated with AmB should be emphasized. In this paper, diammonium glycyrrhizinate (DG) and piperazine ferulate (PF) were taken as the research object to study its hepatoprotective and neuroprotective effect on AmB-induced liver and kidney damage in vitro and in vivo . The microplate method and ELISA kits were employed for the biochemical detection in the serum and urine of mice. Flow cytometric analysis and western blotting analysis were conducted to study the mechanism of DG and PF. Our results confirmed the prevention capacity of DG and PF on AmB-induced liver and kidney injury through the alleviation of pathological changes and enzyme reducing action. Furthermore, DG and PF suppressed ROS-mediated mitochondrial apoptosis in AmB-treated mice and cells through Caspase pathway and Caspase-independent AIF pathway. In summary, DG and PF could protect AmB-induced hepatotoxicity and nephrotoxicity by disrupting oxidative stress and apoptosis.
研究领域:
药理学
·
毒理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）