JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 3
·e70121
·
IF 3.4
Sisi Wu,Xiaomei Cheng,Xinyi Zhang,Yan Liu,Gaoyang Li,Shuangping Chen,Xiangrong Zhu
This study explores the antifungal properties of ferulic acid (FA), ferulic acid methylester (FAM), and ferulic acid ethylester (FAE), against Botryosphaeria dothidea ( B. dothidea ), and their effects on the postharvest quality (decay rate, weight loss rate, total soluble solids, titratable acids, firmness, respiration rate) of “Hongyang” kiwifruit. The results demonstrated that FAE exhibited a stronger in vitro inhibitory effect on B. dothidea than FA and FAM, and offered superior preservation of kiwifruit quality after harvest. Scanning electron microscopy (SEM) images revealed that B. dothidea mycelium treated with FA, FAM, and FAE showed altered surface morphology, with signs of plasmolysis. Transmission electron microscopy (TEM) further confirmed that FAE caused significant damage to the cell membrane and organelles of B. dothidea . Additional analyses using propidium iodide (PI) and calcofluor fluorescence white (CFW) staining, conductivity tests, and alkaline phosphatase (AKP) assays demonstrated that FA, FAM, and FAE treatments compromised cell membrane integrity. In addition, FAE treatment reduced the decay rate of kiwifruit, delayed the softening process and respiratory peak, maintained firmness, and prolonged antioxidant enzyme activity, preserving the fruit's quality over time. These findings suggest that FAE is a potent antifungal agent against B. dothidea with potential applications as an eco-friendly solution for controlling postharvest soft rot in kiwifruit. Practical Application This study explores the antifungal properties of ferulic acid (FA), ferulic acid methylester (FAM), and ferulic acid ethylester (FAE), against Botryosphaeria dothidea , and their effects on the postharvest quality of “Hongyang” kiwifruit. This work provides an eco-friendly solution for controlling postharvest soft rot in kiwifruit.
研究领域:
植物病理学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
Chemistry Central Journal
·
2012
·Vol. 6
·期 1
·1-10
·
IF 3.281
Li Xican,Lin Jing,Gao Yaoxiang,Han Weijuan,Chen Dongfeng
Background As a typical Chinese herbal medicine, rhizoma Cimicifugae (RC, 升麻 in Chinese) possesses various pharmacological effects involved in antioxidant activity. However, its antioxidant activity has not been reported so far. The aim of the present study was to systematically evaluate the antioxidant ability of RC in vitro, then discuss the mechanism. Methods Firstly, five RC extracts (i.e. petroleum ether extract PERC, ethyl acetate extract EARC, absolute ethanol extract AERC, 95% ethanol extract 95ERC, and water extract WRC) were prepared and determined by various antioxidant methods, including anti-lipidperoxidation, protection against DNA damage, ·OH scavenging, ·O 2 - scavenging, DPPH· (1,1-diphenyl-2-picryl-hydrazl radical) scavenging, ABTS + · (2,2’-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid radical ion) scavenging, Cu 2+ -chelating, and Fe 3+ reducing assays. Subsequently, we measured the chemical contents of five RC extracts, including total phenolics, total saponins, total sugars, caffeic acid, ferulic acid and isoferulic acid. Finally, we quantitatively analyzed the correlations between antioxidant levels (1/IC 50 values) and chemical contents. Results In the study, the antioxidant levels and chemical contents (including total phenolics, total saponins, total sugars, caffeic acid, ferulic acid and isoferulic acid) of five RC extracts were determined by various methods. In all antioxidant assays, five RC extracts increased the antioxidant levels in a dose-dependent manner. However, their antioxidant levels (IC 50 values) and chemical contents significantly differed from each other. Quantitative analysis of the correlation showed that total phenolic was of significant positive correlations (average R value was 0.56) with antioxidant levels; In contrast, total sugars and total saponins had no positive correlation with antioxidant (the average R values were −0.20 and −0.26, for total sugars and total saponins, respectively); Among total phenolics, three phenolic acids (caffeic acid, ferulic acid and isoferulic acid) also displayed positive correlations (the average R values were 0.51, 0.50, and 0.51, for caffeic acid, ferulic acid and isoferulic acid, respectively). Conclusions As an effective antioxidant, Rhizoma Cimicifugae can protect DNA and lipids against oxidative damage. Its antioxidant ability can be responsible for its various pharmacological effects and may be mainly attributed to the existence of total phenolics, among which caffeic acid, ferulic acid and isoferulic acid are regarded as main bioactive components. Rhizoma Cimicifugae exerts its antioxidant effect through metal-chelating, and radical-scavenging which is via donating hydrogen atom (H·) and donating electron (e).
研究领域:
中药学
·
药理学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2015
·
IF 1.884
Fuming He,Song Zhang,Xinli Liu
Feruloyl esterase plays an indispensable role in hydrolyzing plant cell walls, and ferulic acid will be released as by-product, which has potential applications in food and medicine industry. This study presents immobilization of partially purified feruloyl esterases from the fermentation liquor of recombinant Pichia on magnetic Fe 3 O 4 nanoparticles. Furthermore, the optimal conditions for the immobilization and some characteristics of immobilized enzyme were studied. The optimal immobilization conditions observed were enzyme 0.2 mg (1 mg/mL, 0.2 mL), magnetic Fe 3 O 4 nanoparticles 4 mg, pH 6.0, immobilization time 3 h. The results showed that the optimal reaction temperature of immobilized enzyme was increased from 45°C to 55°C. Thermal stability of the immobilized enzyme also had an improvement, the residual activity retained 80% and 69% after 120 h at 40°C and 50°C, respectively, while free enzymes only showed 45% and 40% remnant activity at the same condition. The immobilized enzyme also exhibited good operational stability and 52.4% of its initial activity was observed during the fifth cycle. In terms of the thermal and operational stability, the immobilized enzyme could be better used in many more applications than the free enzymes. At last the destarched wheat bran was taken as substrate for immobilized and free feruloyl esterases to produce ferulic acid, the maximum ferulic acid yield was 11.2% and 12.3%, respectively, indicating a great potential in industrial application.
研究领域:
生物技术
·
酶工程
✓ 已验证
DOI
高影响因子
Heliyon
·
2024
·
IF 3.4
Ying Yang,Shuqin Wang,Xingyan Liu,Wenbin Zhang,Wenhua Tong,Huibo Luo,Liming Zhao
Ferulic acid (FA) and ferulic acid methyl ester (FAM) are important phenolic compounds in Baijiu. In this study, the interaction of FA and FAM with human serum albumin (HSA) and lysozyme (LZM) was investigated using multispectral methods and molecular dynamics simulation. FA and FAM could interact with HSA and LZM, changing the conformation and hydrophilicity of the protein. The quenching mechanisms of FA-HSA, FA-LZM, FAM-HSA, and FAM-LZM were all static-quenching. In the FA-HSA, FAM-HSA, and FA-LZM systems, the interaction forces were mainly hydrophobic interactions and hydrogen bonding. In the FAM-LZM system, the interaction forces were mainly hydrophobic interactions, hydrogen bonding, and van der Waals force. Common metal ions such as K + , Ca 2+ , Cu 2+ , Mg 2+ , and Mn 2+ could affect the binding ability of FA and FAM to HSA and LZM. Moreover, FA and FAM could increase the stability of HSA and LZM, and the protein bound to FA/FAM was more stable than the free protein. FA and FAM had varying degrees of impact on the physiological activities of HSA and LZM. This study provides relevant information on the interactions and metabolic mechanisms of FA and its derivatives with endogenous proteins.
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·
IF 3.3
Wenbin Jin,Kaipeng Wang,Xueyang Xu,Yiling Xu,Fengwei Li,qingzhi ji,Xiaodong Chen,Xiaohong Yu
Ferulic acid is a natural phenolic chemical which widely present in plants and widely used in food and cosmetic industries. It is used as the cross-linking agent for the preparation of edible films, and as skin protection agents. Enzymatic hydrolysis of lignocellulose to produce ferulic acid has been studied for many years and is rarely used in the industry due to easy deactivation of enzymes. Deep eutectic solvent (DES) is exploited as a novel co-solvent for improving the activity and stability of enzyme. A mixed enzyme system composed of feruloyl esterase and xylanase was constructed for catalytic degradation of de-starched wheat bran (DSWB) to produce ferulic acid. When the ratio of feruloyl esterase to xylanase reached 1:1, the release of ferulic acid reached the maximum. Four DES was synthesized and evaluated the effects of compositions and concentrations on the release of ferulic acid from DSWB. The mixed enzymatic system showed the best performance in the ChCl-Gly DES aqueous solution (volume ratio of 1:2). Through the determination of the enzymatic properties and catalytic conditions, the mixed enzymatic system was applied to the catalytic reaction. Ferulic acid yield 4.2 mg/g DSWB was obtained under the optimum conditions. The results suggested that the efficient production of ferulic acid from lignocellulosic biomass can be realized through the synergistic effect of feruloyl esterase and xylanase in DES aqueous solution.
研究领域:
生物技术
·
酶学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
PHARMACOLOGY
·
2016
·
IF 1.533
Wang Zhe,Gong Yun,Zeng Da-li,Chen Lian-guo,Lin Gao-tong,Huang Cheng-ke,Sun Wei,Chen Meng-Chun,Hu Guo-xin,Chen Rui-jie
Background: CYP2C9 is one of the most important phase I drug-metabolizing enzymes in liver. The objective of this work was to investigate the effects of apigenin on the metabolism of losartan and human CYP2C9 and rat CYP2C11 activity in vitro. Methods: Different concentrations of apigenin were added to a 100 mmol/l Tris-HCl reaction mixture containing 2 pmol/ml recombinant human CYP2C9.1, 0.25 mg/ml human liver microsomes or 0.5 mg/ml rat liver microsomes to determine the half maximal inhibition or a half-maximal inhibitory concentration (IC50) on the metabolism of losartan. In addition, diclofenac used as CYP2C9 substrate was performed to determine the effects of apigenin on CYP2C9. Results: The results showed that apigenin has the inhibitory effect on the metabolism of losartan in vitro, the IC50 was 7.61, 4.10 and 11.07 μmol/l on recombinant CYP2C9 microsomes, human liver microsomes and rat liver microsomes, respectively. Meanwhile, apigenin's mode of action on human CYP2C9 activity was competitive for the substrate diclofenac. In contrast to its potent inhibition of CYP2C9 in humans (9.51 μmol/l), apigenin had lesser effects on CYP2C11 in rat (IC50 = 15.51 μmol/l). Conclusion: The observations imply that apigenin has the inhibitory effect on the metabolism of losartan and CYP2C9 activity in vitro. More attention should be paid as to when losartan should be administrated combined with apigenin
研究领域:
药理学
·
生物化学
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Shujun Liu,Lubna Soomro,Xue Wei,Xufeng Yuan,Tianyi Gu,Zhen Li,Yulu Wang,Yuming Bao,Fengzhong Wang,Boting Wen,Fengjiao Xin
Producing ferulic acid (FA) from the natural substrate with feruloyl esterase is promising in industries, screening and engineering new enzymes with high efficiency to increase the FA yield is of great concern. Here, the feruloyl esterase of Lactobacillus acidophilus (FAELac) was heterologous expressed and the FAELac with different oligomerization states was separated. Interestingly, the activity of dimer was 37-fold higher than high-polymer. To further enhance the efficiency of FAELac, eight mutants were generated based on the simulated structure, of which Q198A, Q134T enhanced the catalytic efficiency by 5.4- and 4.3-fold in comparison with the wild type. Moreover, higher yields of FA (2.21, 6.60, and 1.67 mg/g substrate, respectively) were released by the mutants from de-starched wheat bran, insoluble wheat arabinoxylan, and steam-exploded corn stover. These results indicated that improving the purification process, engineering new FAELac and substrates bias studies hold great potential for increasing FA production yield.
研究领域:
生物技术
·
酶学
·
生物化学
·
工业生物技术
✓ 已验证
DOI
PubMed
高影响因子
Separation Science Plus
·
2019
·Vol. 2
·期 10
·384-391
·
IF 0
Zhongping Huang,Xiaoli Huang,Huijun Liu,Lili Wang,Yilei Fan,Charles Liu,Yiping Ren
A new method of direct analysis in real-time integrated with mass spectrometry was established and applied to the rapid determination of ferulic acid in Chinese herbs. Instrument parameter settings were optimized to obtain the sensitive and accurate determination of ferulic acid. At the sample introduction speed of 0.5 mm/s, high signal intensity of [M-H] − ( m/z 193) for ferulic acid was achieved in the negative ion mode by utilization of high purity helium gas at 350°C. The calibration curve was linear over the range of 5 – 100 mg/L with the correlation coefficient r = 0.9846 and relative standard deviation (n = 6) in the range of 2.0 to 6.1%. The limit of detection ( S/N = 3) and limit of quantitation ( S/N = 10) were 0.4 and 1.2 mg/L respectively. The recoveries were in the range of 90.6–114.2%. The results of direct analysis in real time-mass spectrometry were in agreement with those obtained by high performance liquid chromatography. The proposed direct analysis in real time mass spectrometry method is a simple, rapid and high-throughput approach for the determination of ferulic acid in Chinese herbs.
研究领域:
中药学
·
分析化学
✓ 已验证
DOI
Foods
·
2023
·Vol. 12
·期 7
·1369
·
IF 5.2
Fenghong Deng,Xiuting Hu,Yueru Wang,Shunjing Luo,Chengmei Liu
Rice bran, rich in feruloyl arabinoxylan, is a good source of feruloyl oligosaccharides (FOs). To prepare FOs, bran was often hydrolyzed by amylase and protease to remove starch and protein and then hydrolyzed by xylanase, which was time-consuming and had a low yield. To solve the above problems, enzymatic extrusion was used to treat rice bran, and the effects of traditional hydrolysis, a combination of traditional extrusion and hydrolysis (extrusion-hydrolysis) and enzymatic extrusion on the yield of FOs were investigated and compared in this study. It was found that traditional extrusion and enzymatic extrusion significantly increased the yield of FOs. Particularly, the yield of FOs resulting from enzymatic extrusion was increased to 5.78%, while the yield from traditional hydrolysis was 4.23%. Microscopy analysis showed that extrusion damaged the cell wall of bran, which might increase the accessibility of xylanase to arabinoxylan and the yield of FOs. Spectroscopy analysis suggested that FOs obtained by different pretreatments had similar structures. It was obvious that enzymatic extrusion saved the time for removal of starch and protein and increased the yield of FOs. In addition, the highest yield of FOs was found at the moisture content of 30% and the screw speed of 50 rpm. This study provided an efficient method for the preparation of FOs that is suitable for industrial production.Keywords:rice bran;feruloyl oligosaccharides;enzymatic extrusion;yield
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hongling Wang,Xianghui Qi,Song Gao,Guoshi Kan,Lkhagvasuren Damdindorj,Yingfeng An,Fuping Lu
Herein, a novel multifunctional enzyme β-glucosidase/xylanase/feruloyl esterase (GXF) was constructed by fusion of β-glucosidase and bifunctional xylanase/feruloyl esterase. The activities of β-glucosidase, xylanase, feruloyl esterase and acetyl xylan esterase displayed by GXF were 67.18 %, 49.54 %, 38.92 % and 23.54 %, respectively, higher than that of the corresponding single functional enzymes. Moreover, the GXF performed better in enhancing aroma and quality of Longjing tea than the single functional enzymes and their mixtures. After treatment with GXF , the grassy and floral odors of tea infusion were significantly improved. Moreover, GXF treatment could improve concentrations of flavonoid aglycones of myricetin, kaempferol and quercetin by 68.1-, 81.42- and 77.39-fold, respectively. In addition, GXF could accelerate the release of reducing sugars, ferulic acid and xylo -oligosaccharides by 9.48-, 8.25- and 4.11-fold, respectively. This multifunctional enzyme may have potential applications in other fields such as food production and biomass degradation.
研究领域:
酶学
·
食品科学
·
茶叶技术
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2020
·
IF 2.734
Bo Yu,Ying Pu,Jun Liu,Jie Liao,Ke Chen,Jiani Zhang,Wen Zhong,Yalan Hu,Xue-Qiang Wang,Bo Liu,Huixia Liu,Weihong Tan
Targeted delivery of antiobesity drugs to adipocytes presents a novel strategy for obesity treatment. The 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in adipose tissue is an attractive therapeutic target of obesity. Emodin (EMO) has been proven to be a potent and selective inhibitor of 11β-HSD1, but it frequently exerts low bioavailability owing to its poor water solubility and lack of tissue specificity. In this work, we conjugated a nanoscale drug delivery polymer with Adipo8 (Ap), a DNA aptamer with high affinity to mature white adipocytes, as a targeting modality that enabled selective delivery of emodin to adipocytes. Emodin-loaded PEG-PLGA nanoparticles (EMO-NPs) were formulated by a modified oil-in-water (O/W) emulsion solvent evaporation method, and Ap was covalently bound to the NP surface via the EDC/NHS method. The NPs were structurally investigated by TEM imaging, DLS and UV-VIS spectroscopy. The resulting aptamer-conjugated, emodin-loaded nanoparticles (Ap-EMO-NPs) had a spherical shape and an average particle size of 146.7 ± 27.85 nm with a drug loading of around 6.8% and a sustained-release property. Confocal microscopy and flow cytometry demonstrated a significant increase in the internalization of Ap-EMO-NP in differentiated 3T3-L1 cells compared to EMO-NP functionalized with nonspecific aptamer. As confirmed by Oil Red O coloring, the Ap-EMO-NPs reduced lipid stacking in 3T3-L1 adipocytes in a sustained-release and dose-dependent manner. The results indicated that Adipo8-functionalized PEG-PLGA NPs could be a potential targeted therapeutic delivery vehicle for obesity treatment.
研究领域:
药理学
·
生物医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 29
·16701-16712
·
IF 3.049
Xiaofeng Li,Maohua Ma,Xuan Xin,Yuqian Tang,Guanglei Zhao,Xinglong Xiao
Gastrodin, a bioactive compound extracted from the plant source of Gastrodia elata Blume, has a wide range of therapeutic effects on central nervous system (CNS) diseases, but suffers from poor brain permeability and short half-life in plasma. In this study, fatty acid esters of gastrodin were successfully synthesized by a whole cell-based biocatalytic method. Aspergillus oryzae cells showed different catalytic activities in the organic solvent systems tested. Tetrahydrofuran was confirmed as the most suitable pure organic solvent, with the highest substrate conversion of 98.0%. Addition of ionic liquids (ILs) into pyridine dramatically accelerated the reaction with conversion increased from 5.9% to 84.2%, and also changed the selectivity of the cells, mainly due to the use of IL-containing systems altering cell permeability and contact of the enzymes with solvent molecules possessing different polarities. The ester products were characterized by 13C-NMR and ESI-MS as gastrodin monoester and diester.
研究领域:
药理学
·
生物催化
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）