JOURNAL OF CEREAL SCIENCE
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2018
·
IF 2.302
Kuijie Gong,Lirong Chen,Xiaoyue Li,Linlin Sun,Kaichang Liu
A combination of germination and extrusion was applied to whole grain corn to increase its nutritional composition, functional properties and non-anthocyanin polyphenol profile, especially related to in vitro hypoglycemic effects. Individual germination and extrusion both led to an increase in soluble dietary fiber and lysine, methionine , tryptophan content, while insoluble dietary fiber content decreased. Extruded germinated whole corn flour (EGWCF) had a higher water absorption and solubility index, showing 103 and 575% increases over untreated ones, respectively. Germination increased free, bound phenolic acid and bound flavonoid content to 169, 230 and 311%, respectively. The total phenolic acid and flavonoid content of EGWCF was 260 and 249% of WCF, respectively. Germination increased the polyphenol composition from 12 to 15 species. Phenolic profiles of EGWCF exhibited collective effects, showing three new phenolic compounds . Polyphenol extracts showed more potent activity against α -glucosidase rather than α -amylase. Free form polyphenols had stronger inhibitory activity than the bound forms. Compared with WCF, free and bound polyphenol extracts from EGWCF increased the anti- α -glucosidase activity by 221 and 40%, and increased the anti- α -amylase activity by 105 and 108%.
研究领域:
食品科学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Simin Feng,Di Luan,Ke Ning,Ping Shao,Peilong Sun
Brasenia schreberi ( B. schreberi ) is a rare and precious vegetable, which coat with a gelatinous mucilage. This study is aiming to investigate the relationship between structural characteristics and hypoglycemic activities of polysaccharides with different molecular weight ( Mw ) range. Three polysaccharides fractions, namely BSP-U100 (50–100 kDa), BSP-U50 (10–50 kDa) and BSP-U10 (<10 kDa), were isolated from B. schreberi mucilage using ultrafiltration method. Compared to other polysaccharide samples, only BSP-U100 had a triple helix structure and existed as the ordered structure in aqueous solution. Furthermore, BSP-U100 exhibited higher α-amylase (IC50 = 0.4414 mg/mL) and α-glucosidase (IC50 = 0.5993 mg/mL) inhibitory activity. A heteropolysaccharide BSP-1a, which average Mw was 83.98 kDa, was purified from BSP-U100 by DEAE Sepharose Fast Flow and Sephadex G-75 column. D-galactose (66.85%), d -glucose (2.05%) and D-mannose (1.65%) were three main monosaccharides of BSP-1a. Structural characterization of BSP-1a showed that BSP-1a might be a kind of L-D-pyranose type galactose and mainly composed of α -1 → 6-linked Galp, α -1 → 3-linked Manp and α -1 → 4-linked Glcp. This study provided a theoretical basis for the development and application of B. schreberi polysaccharides as a potential anti-diabetic supplement.
研究领域:
植物化学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
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2022
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IF 4.885
Yaling He,Hui Chen,Ziyang Ye,Xinmiao Zhang,Hongling Ye,Ming Ye
To prepare zinc and selenium modified polysaccharides derivatives (ZnLEP-1a and SeLEP-1a), a novel polysaccharide (LEP-1a) isolated from Lachnum YM38 was chosen. Following that, the structural characterization and in vitro bioactivities of LEP-1, ZnLEP-1a and SeLEP-1a were investigated. Results revealed that LEP-1a had a molecular weight of 5.69 × 10 4 Da and was composed of mannose , galactose and glucose. The linkage types of LEP-1a were proved to be (1→)-α- D -Manp, (1→3)-α- D -Manp, (1→2)-α- D -Galp, (1→2)-α- D -Glcp, (1→2,6)-α- D -Manp. Zinc content of ZnLEP-1a was 0.69 mg/g, while selenium content of SeLEP-1a was 206.99 µg/g. Compared with LEP-1a, ZnLEP-1a and SeLEP-1a had a lower molecular weight, same monosaccharides in different molar ratios. The results of FT-IR, DSC , NMR, SEM and AFM suggested that there were obvious changes in structural characteristics after LEP-1a was modified with zinc and selenium. ZnLEP-1a and SeLEP-1a had stronger antioxidant, hypoglycemic, antitumor activities in vitro in comparison with LEP-1a. As a result, ZnLEP-1a and SeLEP-1a showed great potential to develop into a natural antioxidant, hypoglycemic and low-toxic antitumor drug.
研究领域:
生物化学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
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2025
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IF 3.3
Wu Yingying,Liu Yanan,Wang Shanshan,Zhang Yuhong,Hou Dianzhi,Ren Feiyue,Zhou Sumei
The study aimed to examine the nutritional composition, polyphenolic content, antioxidant properties and hypoglycemic activity of ten land-race colored highland barley varieties. The results indicated that highland barley exhibited significant levels of protein (ranging from 9.33 to 16.71%), fiber (ranging from 11.59 to 15.26%), and β-glucans (ranging from 4.07 to 6.42%), with comparatively lower fat content (ranging from 1.73 to 2.79%). The colored barley exhibited a noteworthy concentration of polyphenols, whereas the yellow barley variety 1128 displayed surprisingly elevated levels of polyphenols. The identified polyphenols encompassed naringenin, salicylic acid in the unbound fraction, and ferulic acid, epicatechin, and salicylic acid in the bound fraction across all ten varieties. Both the free and bound fractions of polyphenols in highland barley demonstrated exceptional antioxidant activity. Nevertheless, there is currently limited data regarding the hypoglycemic effects of highland barley polyphenols. In this study, it was determined that the polyphenols found in highland barley possess the capability to impede the enzymatic activities of α-amylase and α-glucosidase. The individual phenolic compounds significantly contributed to the antioxidant capacity, as well as the inhibition of α-amylase and α-glucosidase. These findings suggest that the ten varieties of highland barley hold promise as a functional supplement for regulating blood glucose levels and preventing diabetes.
研究领域:
食品科学
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植物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2018
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IF 4.946
Lei Wang,Bin Zhang,Jie Xiao,Qiang Huang,Chao Li,Xiong Fu
Water-soluble polysaccharides (RTFP) were extracted from Rosa roxburghii Tratt fruit by hot water method. The physicochemical, functional, and hypoglycemic properties of RTFP were investigated. The results revealed that RTFP mainly contained carbohydrates (63.79 ± 0.73%, g/g), uronic acids (14.8 ± 0.06%, g/g), and proteins (4.10 ± 0.58%, g/g). RTFP was composed of arabinose, galactose, glucose, mannose, xylose, and fucose with molar percentages of 33.8, 37.3, 20.7, 1.74, 3.43, and 2.95%, respectively. Functional analyses indicated that RTFP had good oil-holding capacity, foaming properties, and emulsifying capacity. The rheological results showed that RTFP exhibited typical shear-thinning behavior and viscoelastic properties influenced by sample concentration, temperature and inorganic salt. Additionally, RTFP exhibited favorable inhibitory activities against α-glucosidase in a mixed inhibition type, and against α-amylase in an uncompetitive inhibition type. These results suggest that RTFP can be exploited as a multi-functional additive or hypoglycemic agent in foods, pharmaceuticals and cosmetics.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
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2023
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IF 8.4
Zhiming Li,Shu Zhang,Lu Bai,Huacheng Tang,Guifang Zhang,Jiayu Zhang,Weihong Meng,Dongjie Zhang
The self-assembled structures of coix seeds affected the enzymatic efficiency and doesn‘t facilitate the release of more active peptides. The influence of heating combined with ultrasound pretreatment (HT + US) on the structure, enzymatic properties and hydrolysates (CHPs) of coix seed prolamin was investigated. Results showed that the structural of coix seed prolamins has changed after HT + US, including increased surface hydrophobicity, reduced α-helix and random coil content, and a decrease in particle size. So that, leads to changes in thermodynamic parameters such as an increase in the reaction rate constant and a decrease in activation energy, enthalpy and enthalpy. The fractions of <1000 Da, degree of hydrolysis and α-glucosidase inhibitory were increased in the HT + US group compared to single pretreatment by 0.68%–17.34%, 12.69%–34.43% and 30.00%–53.46%. The peptide content and α-glucosidase inhibitory activity of CHPs could be maintained at 72.21 % and 57.97 % of the initial raw materials after in vitro digestion. Thus, the findings indicate that HT + US provides a feasible and efficient approach to can effectively enhance the enzymatic hydrolysis efficiency and hypoglycaemic efficacy of CHPs.
研究领域:
食品科学
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生物技术
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酶学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Yi-Long Ma,Zi-Tong Wang,Dong-Mei Yang,Zhi Li,Qian-Lan Wu,Xin Guo,Ya-Fang Shang,Kiran Thakur,Zhao-Jun Wei
The well- known winter jujube ( Zizyphus jujuba Mill. cv. Dongzao), has been proved to contain a series of bioactive compounds. To investigate the impact of processing and digestion on its phenolics, winter jujube was processed (steaming and freeze-drying) and digested. The treated winter jujubes' phenolic content, antioxidant capacity, and hypoglycemic potential were assessed. The results showed that processing reduced the number of total phenolics/flavonoids, antioxidant, and α-glucosidase inhibitory activity by 0.74–25.26%. With the exception of α-glucosidase inhibitory activity, which increased by 16.67–19.25% after digestion, these declines were more pronounced. After processing, the six discovered phenolics in winter jujube were mostly constant, but after digestion, their contents dropped by 6.72–38.64 %. These results suggested that following processing and digestion, the phenolic content and activity of winter jujube remained high (61.36–97.93%), indicating that freeze-drying and steaming are viable approaches to prepare and consume winter jujube.
研究领域:
食品科学
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植物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
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2017
·Vol. 22
·期 10
·1562
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IF 2.861
Tai-Mei Di,Shao-Lan Yang,Feng-Yu Du,Lei Zhao,Tao Xia,Xin-Fu Zhang
One new and three known triterpenoid saponins were isolated and identified fromCamellia oleiferaseeds through IR, NMR, HR-ESI-MS and GC-MS spectroscopic methods, namely oleiferasaponin A3, oleiferasaponin A1, camelliasaponin B1, and camelliasaponin B2. The structure of oleiferasaponin A3was elucidated as 16α-hydroxy-21β-O-angeloyl-22α-O-cinnamoyl-23α-aldehyde-28-dihydroxymethylene-olean-12-ene-3β-O-[β-d-galactopyranosyl-(1→2)]-[β-d-xylopyranosyl-(1→2)-β-d-galactopyranosyl-(1→3)]-β-d-gluco-pyranosiduronic acid. Camelliasaponin B1and camelliasaponin B2exhibited potent cytotoxic activity on three human tumour cell lines (human lung tumour cells (A549), human liver tumour cells (HepG2), cervical tumour cells (Hela)). The hypoglycemic activity of oleiferasaponin A1was testified by protecting pancreatic β-cell lines from high-glucose damage.Keywords:Camellia oleifera;triterpenoid saponin;oleiferasaponin A3;cytotoxic activity;hypoglycemic activity
研究领域:
植物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Li Tang,Xiaomin Luo,Meiyuan Wang,Zhong Wang,Juan Guo,Fansheng Kong,Yongguang Bi
Under a simple redox system of selenite and ascorbic acid , we used Gracilaria lemaneiformis polysaccharides (GLPs) as a stabilizer and dispersing agent to generate well-dispersed and stable selenium nanoparticles (SeNPs). The size, stability, morphology and physicochemical properties of GLPs-SeNPs were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Ultraviolet-visible spectroscopy (UV–vis), Fourier transform infrared (FT-IR) spectra, energy dispersive X-ray (EDX), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Thermogravimetric (TG). The results showed that orange-red, amorphous, zero-valent and spherical GLPs-SeNPs with mean diameter of approximately 92.5 nm were successfully prepared, which exhibited good storage stability at 4 °C and remaining highly stable at different ion strengths and pH. The 2,2-diphenyl-1-pycrylhydrazyl (DPPH), 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and superoxide anion radical (O 2 •− ) radical scavenging ability of GLPs-SeNPs were higher than those of bare SeNPs, GLPs and sodium selenite (Na 2 SeO 3 ), and could reach 103.41%, 94.23%, 86% at a concentration of 1.5 mg/mL, respectively. Besides, GLPs-SeNPs also showed higher inhibitory effects on α-amylase and α-glucosidase. In vitro cytotoxicity assay and hemolysis activity examinations indicated that GLPs-SeNPs have excellent biocompatibility. Therefore, the GLPs-SeNPs might be used as a potential antioxidant agent and antidiabetic agent for food and medical applications.
研究领域:
食品科学
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生物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Ma Quantao,Li Yaqi,Zhao Ruixue,Tang Ziyan,Li Jialin,Chen Cong,Liu Xiaoyao,Hu Yujie,Wang Ting,Zhao Baosheng
Background: Type 2 diabetes (T2D) is considered as one of the most significant metabolic syndromes worldwide, and the long-term use of the drugs already on the market for T2D often gives rise to some side effects. The mulberry leaf (ML), Morus alba L., has advantages in terms of its comprehensive therapeutic efficacy, which are characterized as multicomponent, multitarget, multipathway, and matching with the complex pathological mechanisms of diabetes.Methods: T2D rats were established by a high-fat diet combined with an intraperitoneal injection of streptozotocin; an evaluation of the hypoglycemic effects of the ML in combination with fasting blood glucose and other indicators, in addition to the utilization of metabolomics technology, was performed to analysis the metabolite changes in serum of rats.Results: MLs significantly reduced the fasting blood glucose of T2D rats, while improving the symptoms of polyphagia and polyuria. ML treatment altered the levels of various metabolites in the serum of T2D rats, which are involved in multiple metabolic pathways (amino acid metabolism, carbohydrate metabolism, and lipid metabolism), played a role in antioxidative stress and anti-inflammation, modulated immune and gluconeogenesis processes, and improved obesity as well as insulin resistance (IR).Conclusion: The ML contains a variety of chemical components, and metabolomic results have shown that MLs regulate multiple metabolic pathways to exert hypoglycemic effects, suggesting that MLs may have great promise in the development of new hypoglycemic drugs.
研究领域:
药理学
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代谢组学
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糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 19
·3082
·
IF 4.7
Chuan Liu,Yu Miao,Wenjia Zhou,Yiming Ma,Wenkui Guo,Aili Li
In this study, three kinds of thermal treatments were applied to sweet potatoes: steaming (100 °C, 20 min), frying (150 °C, 10 min), and baking (200 °C, 30 min). We analyzed the changes in the physicochemical structure, antioxidant properties, and hypoglycemic activities of sweet potato polysaccharides between untreated and heat-treated samples. The results showed that the polysaccharides of all sweet potatoes (untreated and heat-treated) were composed of pyranose structures, had low protein content, and shared the same monosaccharide composition. Infrared spectra showed that the three thermal processing treatments had no significant effect on the functional groups or chemical bonding of sweet potato polysaccharides. In addition, all four polysaccharides exhibited dose-dependent antioxidant and hypoglycemic activities. The above experimental results suggest that thermal processing did not affect the physicochemical, antioxidant, or hypoglycemic activities of sweet potato polysaccharides.
研究领域:
食品科学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Sheng Zhang,Xiangzhou Li
We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro . The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose :Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125–0.500 mg/mL. Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration. A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established. This result suggests that CCP can be used as hypoglycemic medicine or health food.
研究领域:
生物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）