ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Jiarong Su,Peishan Li,Xinrui Tang,Zihao Chen,Qiang Peng,Huilin Li,Qianhua Zhang,Shengjie Lu,Famin Wen,Huaicheng Qin,Yongguang Bi,Juan Guo,Fansheng Kong,Haijie Jin,Canzhao Chen,Huiyan Xu
Plant polyphenols have gained increasing attention due to their abundant and powerful biological activities. However, polyphenols from roots of Ilex asprella (RIAPs) remain largely unexplored, with limited research on their composition and bioactivity, making it necessary to focus on RIAPs. In the present study, single - factor analysis and response surface optimization were performed for the extraction of RIAPs via an ultrasonic bath extraction method. The findings demonstrated that at 50 % ethanol concentration, 30 min of ultrasonic time, and 300 W of ultrasonic power, the maximum extraction content of RIAPs was 30.14 ± 0.41 mg/g. Compound and structural analysis via high-resolution liquid chromatography-mass spectrometry (LC-MS/MS) and a Fourier transform infrared (FT-IR) spectrometer confirmed that polyphenols were the majority of compounds comprising RIAPs, in addition to small amounts of alkaloids and trace amounts of other compounds such as amino acids. Scanning electron microscope (SEM) images revealed that RIAPs exhibited a densely reticulated and fragmented microstructure. In addition, bioactivity experiments demonstrated significant antioxidant, antibacterial, anti-inflammatory, and anti-hyperlipidemic activities of RIAPs, supporting their potential pharmaceutical applications. This study provides an experimental foundation for further pharmacological research and potential drug development of RIAPs.
研究领域:
植物学
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生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lulu Zhao,Yongbo Peng,Jing Huang,Nishang Liu,Xinrong Zou,Junnan Li,Yunpeng Fan,Ping Li,Liling Tang,Jisheng Wang,Yajun Zeng,Yi Wu,Gaohui Zhu
Polysaccharides are the major bioactive composition of Polygonatum sibiricum ( P. sibiricum ). However, the structural and functional identifications of these polysaccharides were still limited. Herein, we isolated a novel P. sibiricum polysaccharides (PSPF) and explored its potential function and mechanism in alleviating hyperlipidemia. PSPF were purified by diethylaminoethyl-sepharose fast flow (DEAE-Sepharose FF) and cross-linked dextran gel LH-20 (Sephadex LH-20) column chromatography, and identified by gel-permeation chromatography, methylation analysis, fourier transform infrared spectrometer (FT-IR), nuclear magnetic resonance (NMR), and gas chromatography–mass spectrometry (GC–MS). Their molecular weight (Mw), polysaccharide composition, and chemical structure were characterized. Furthermore, egg yolk emulsion-induced acute hyperlipidemia mouse model was constructed to evaluate the lipid-lowering efficacy and the underlying mechanism of PSPF. It was found that PSPF, with the Mw of 3592 Da, were prepared and mainly consisted of fructan with →1)-β-D-Fru f -(2 → main chain and →6)-β-D-Fru f -(2 → side chains. In addition, PSPF supplements efficiently reduced liver lipid accumulation, alleviated hepatocyte steatosis, and upregulated the AMP-activated protein kinase (AMPK) pathway, thereby enhancing fatty acid oxidation and decomposition. These results indicate that PSPF may serve as the potential dietary supplements for lipid reduction.
研究领域:
生物化学
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营养学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shu-Min Hu,Jia-Min Zhou,Qing-Qing Zhou,Ping Li,Yuan-Yuan Xie,Tao Zhou,Qing Gu
Exopolysaccharides (EPS) produced by Lactobacillus rhamnosus ZFM231 isolated from milk were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence, obtaining four purified fractions EPS-1, EPS-2, EPS-3 and EPS-4. Among them, EPS-1 was a neutral polysaccharide and the others were acidic polysaccharides. The four factions were characterized by chemical composition analysis, molecular weight measurement, and UV, IR, 1 H and 13 C NMR spectra analysis. The antioxidant activity assays indicated that the purified fractions possessed appreciable ferric iron reducing power, free radical (DPPH, hydroxyl and superoxide anion radicals) scavenging ability and ferrous iron chelating ability. These purified exopolysaccharides were also found to possess marked hypolipidemic activity in vitro . The results showed that they were able to effectively inhibit lipase activity, and possessed remarkable activity in reducing cholesterol and triglyceride levels in the HepG2 cell models. Therefore, Lactobacillus rhamnosus ZFM231 and EPS produced by this probiotic strain may find application in food and pharmaceutical industries.
研究领域:
食品科学
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微生物学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2013
·Vol. 18
·期 4
·3733-3744
·
IF 2.428
Yu Cao,Yang Zhang,Xiaoning Wang,Bo Yang,Yonghua Wang
Structured lipids (SLCTs triacylglycerols with short- and long-chain acyl residues) were synthesized by interesterification of triacetin and fatty acid methyl esters (FAMEs) from camellia oil, followed by molecular distillation for purification. Different commercial immobilized lipases (Lipozyme RM IM and Novozyme 435), the substrate molar ratios of FAMEs to triacetin, the reaction temperatures and the lipase amounts were studied for their efficiency in producing SLCTs. Results showed that Novozyme 435 was more suitable for this reaction system. Moreover, the optimal reaction conditions for the highest conversion of FAMEs and the highest LLS-TAGs (triacylglycerols with one short- and two long-chain acyl residues) yields were achieved at a molar ratio of FAMEs to triacetin of 3:1, 50 °C of reaction temperature and a lipase amount of 4% (w/v). Scale-up was conducted based on the optimized reaction conditions. Results showed that after 24 h of reaction , the conversion rate of FAMEs was 82.4% and the rate of disubstituted triacetin was 52.4 mol%. The final product yield rate was 94.6%. The effects of the synthesized SLCTs on the plasma lipid level of fasting mice were also studied. The SLCTs could effectively lessen the total triacylglycerol levels in plasma compared to the triacylglycerol group in fasting NIH mice. It suggested that this type of structured lipid might be beneficial for human health, especially for the prevention of obesity.Keywords:structured lipids;lipase;fasting test;interesterification;triacylglycerol
研究领域:
生物化学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2018
·Vol. 34
·期 4
·571-577
·
IF 1.248
Han Xie,Shao Kaiyuan,Hu Wenxiang
Hyperglycemia is frequently accompanying with hyperlipidemia. To explore the potent drugs with dual-activity and dual-site effects that could reduce blood glucose and blood lipid at the same time, fibrate group with lipid-lowering effect on the 9th position of berberine(BBR) was introduced using the drug design combination principle and the multitarget collaborative treatment method. Moreover, the molecular structure of BBR was modified, and six 9-substituted derivatives of BBR were designed and synthesized, among which, five compounds have never been reported before. In addition, the molecular structures of these derivatives were identified using liquid chromatography-mass spectrometry(LC-MS), 1 H nuclear magnetic resonance( 1 H NMR) and 13 C NMR, respectively. Furthermore, the microwave irradiation experimental technique was applied in the synthesis reaction using the novel microwave synthesizer, which accelerated the reaction rate, enhanced the reaction yield, reduced the reaction by-products, and simplified the post-processing steps. In the meantime, the 9-position regioselective demethylation of BBR was explored through quantum chemical calculation during the synthesis of berberrubine. The computations were consistent with the experimental results, which contributed to deducing the mechanism of its selective methylation.
研究领域:
药物化学
·
合成化学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hui Zhang,Guilin Chen,Yongli Zhang,Mei Yang,Jinming Chen,Mingquan Guo
Leaf of Nelumbo nucifera Gaertn. ( N. nucifera ) has been widely used as the main ingredient in lipid-lowering herbal teas and some prescriptions in China due to their excellent hypoglycemic and hypolipidemic effects. However, the active components responsible for these beneficial properties and their mechanisms remain unexplored. In this work, the N. nucifera leaf extracts significantly promoted the glucose consumption of HepG2 cells, and also exhibited remarkable inhibitory activities against α-glucosidase, pancreatic lipase, and COX-2. Furthermore, the top four potential active compounds ( N -nornuciferine, Nuciferine, 2-Hydroxy-1-methoxyaporphine, and Isorhamnetin 3-O-glucoside) targeting the above three enzymes were screened out by bioaffinity ultrafiltration with multiple targets coupled with HPLC-MS/MS. The enzyme inhibitory activities of candidate compounds were verified by enzyme inhibition assay and molecular docking. In addition, molecular docking revealed the binding information between the candidate molecules and enzymes. The current study provided valuable information in discovering functional active ingredients from complex medicinal plant extracts.
研究领域:
药理学
·
生物化学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Miao-Miao Wang,Fan Wang,Gen Li,Meng-Ting Tang,Chong Wang,Qing-Qing Zhou,Tao Zhou,Qing Gu
In order to make full use of the citrus resource, antioxidant and hypolipidemic activities of pectin isolated from citrus fruit canning processing water were investigated. The crude citrus pectin (CLCP) and its three separated fractions (LCP0, LCP1 and LCP3) possessed considerable DPPH, hydroxyl and ABTS radical scavenging ability. These were also found to effectively decrease ROS level and improve SOD activity in the H 2 O 2 -induced oxidative stress HepG2 cell model. In an in vitro simulated gastrointestinal environment, all the four pectins showed remarkable bile acid-binding ability. CLCP, LCP1 and LCP3 exhibited appreciable activity in reducing total cholesterol (TC) and triglyceride (TG) levels in an oleic acid-induced high-fat HepG2 cell model. RT-qPCR assays revealed that pectins effectively down-regulated the mRNA expression level of fatty acid synthetase (FAS) and sterol regulatory element-binding protein (SREBP)-1c. Thus, down-regulation of mRNA expression FAS and SREBP-1c could be important mechanism of the pectins to lower blood lipids. The citrus pectins investigated in this work have potential as ingredients of functional foods due to the antioxidant and hypolipidemic activities.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Mingjing Zheng,Huan Ouyang,Zhipeng Li,Tao Hong,Yanbing Zhu,Yuanfan Yang,Xiaoming Guo,Hui Ni,Zedong Jiang
The structure and in vitro hypolipidemic activity of Bangia fusco-purpurea polysaccharide (BFP) assisted extracted with ultra-high pressure (UHP) at 100–600 MPa were studied. Compared to native BFP, UHP assisted extracted BFP had a more loose network structure with higher total sugar and uronic acid contents while less molecular weight ( p < 0.05). Moreover, UHP assisted extraction significantly improved the in vitro hypolipidemic and antioxidant activity of BFP. Especially at 400 MPa UHP, the cholesterol adsorption and antioxidant capacities of BFP were increased by approximately 38.02 % and 11.69 %–32.29 %, respectively. BFP with UHP assisted extraction could alleviate oleic acid-induced lipid accumulation and lipid oxidation in HepG2 cells more effectively by activating the AMPK signaling pathway as well as inhibiting PPARγ expression, which was much related with its reduced molecular weight and loose network structure. The findings indicated that UHP assisted extracted BFP has better potential to develop natural hypolipidemic agent.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Dong-Dong Li,Pan Yu,Zhen-Zhong Wang,Wei Xiao,Xin-Hu Zhou,Lin-Guo Zhao
Berberine (BBR), a well-known alkaloid, exhibits various pharmacological activities, especially hypolipidemic activity, which has attracted much interest from medicinal chemists in the past decade. However, little progress was made on the structural modification of BBR for improving lipid-lowering activity, mainly due to its unclear biological target and low safety. In this study, a new scaffold of 7,9-disulfatetrahydroberberine was discovered unexpectedly, provided with extremely low cytotoxicity. Hence, a novel series of highly safe 7,9-disulfatetrahydroberberines were designed, synthesized, and evaluated for their hypolipidemic activities. In order to investigate the significance of the 9-position substituent, another new series of 7-sulfatetrahydroberberines were designed and synthesized. Lipid-lowering experiments showed that among these compounds, 5f exhibited the best lipid-lowering activity based on two cell models, 3T3-L1 cells and HepG2 cells. Compared with the blank control, the inhibition rate of compound 5f against total cholesterol was over 60%, the inhibition rate against triglyceride was over 70%, the inhibition rate against low-density lipoprotein cholesterol was approximately 75%, and the inhibition rate against high-density lipoprotein cholesterol was close to 50%, which were far superior to the positive control BBR. This result also verified the feasibility of the development of BBR as a lipid-lowering drug via disubstituted modification at the 7- and 9-position.
研究领域:
药物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2025
·
IF 3.3
Zang Yanqing,Chuang Yingying,Wang Changyuan,Cao Yang
The aim of this study was to investigate the optimal process conditions for the preparation of white quinoa polysaccharides and the bioactivity of white quinoa polysaccharides in vitro. This study used white quinoa as raw material and utilized ultrasound-assisted extraction to extract polysaccharides from white quinoa. The optimal extraction technology for white quinoa polysaccharides (WQP) was studied through response surface methodology (RSM) experiments. In addition, preliminary characterization of WQP and determination of their physicochemical properties were conducted. Determined the content of nutrients in WQP. The hypoglycemic, antioxidant, and lipid-lowering effects of WQP were evaluated by different in vitro experiments. The results showed that the yield of WQP was 2.11% under the optimal process. WQP inhibited α-glucosidase and α-amylase by 60.85% and 51.30%, respectively. The scavenging rates of ▪OH, DPPH, ABTS, and ▪O 2 − radicals were 48.36%, 67.17%, 74.87%, and 61.26% respectively. In addition, WQP has some choline-binding ability. It also inhibited cholesterol lipase and pancreatic lipase by 49.76% and 80.57% respectively. The present study demonstrated the glucose-lowering, antioxidative and lipid-lowering effects of white quinoa polysaccharide in vitro.
研究领域:
食品科学
·
生物化学
·
药理学
·
营养学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Chunqi Lu,Qiuya Gu,Xiaobin Yu
Fucoidan, a marine-derived sulfated polysaccharide, exhibits a broad spectrum of biological activities. However, its high molecular weight and viscosity often restrict these benefits. In this study, a new fucoidanase-producing microbial strain Kosakonia oryzendophytica LH-58 was isolated from freshwater for the first time. Three low-molecular-weight fucoidans were synthesized through enzymatic hydrolysis, and their antioxidant, hypoglycemic, hypolipidemic, and anticoagulant properties were evaluated in vitro . With the hydrolysis of fucoidan by fucoidanase, there was no significant change in the composition of monosaccharides, and the sulfate content decreased slightly. Interestingly, reduced molecular weight enhanced antioxidant activity, though anticoagulant efficacy diminished. In vitro experiments demonstrated that a higher molecular weight of fucoidan significantly prolonged activated partial thromboplastin and thrombin times. The fraction F1, with a weight-average molecular weight exceeding 10 kDa, displayed superior hypoglycemic and lipid-lowering effects compared to its counterparts. The IC 50 values for F1's inhibition of α-amylase and α-glucosidase were 0.81 mg/mL and 0.94 mg/mL, respectively, while its binding rates to sodium taurocholate and sodium glycocholate were 50.11% ± 2.32% and 54.58% ± 2.71%, respectively. This study also offers a methodological reference for the swift identification of functional polysaccharides to prevent atherosclerosis.
研究领域:
海洋生物学
·
药理学
·
生物化学
✓ 已验证
DOI
高影响因子
Journal of Analysis and Testing
·
2024
·
IF 5.5
Zhang Hui,Chen Guilin,Zhang Yongli,Muema Felix Wambua,Ding Jun,Guo Mingquan
With the growing prevalence of diabetes and obesity worldwide, the role of α -glucosidase and lipase inhibitors has gained increasing concentration. Mulberry, a functional medicinal and edible plant, has been reported extensively in alleviating both diabetes and obesity. However, there is still a lack of refined research on its active ingredients and mechanisms. In our study, the inhibitory activities of mulberry leaf against α -glucosidase and pancreatic lipase reached 191.37 ± 14.46 and 775 ± 68.60 μg/mL, respectively. Meanwhile, it also promoted glucose consumption in the HepG2 cells model. Subsequently, four α -glucosidase ligands and four pancreatic lipase ligands were identified through bio-affinity ultrafiltration combined with liquid-chromatography-mass spectrometry (UF-HPLC–MS/MS). In vitro inhibition assays confirmed the inhibitory potential of these representative ligands. Molecular docking suggested that these possible ligands could be inserted into the active pocket of the target enzyme through multiple intermolecular forces. Our findings indicated that the polyphenols in mulberry leaves may contribute significantly to their hypoglycemic and hypolipidemic properties. Furthermore, our study provides valuable insights for the screening of active ingredients in other medicinal and edible plants, which could help in the development of new therapies for diabetes and obesity.
研究领域:
药理学
·
生物化学
·
药物化学
·
植物科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）