Analytical Methods
·
2016
·Vol. 8
·期 7
·1577-1585
·
IF 1.915
Tao Liao,Fang Yuan,Haoyang Yu,Zigang Li
Sepsis is a potentially fatal systemic body infection with a significant mortality rate worldwide. Procalcitonin (PCT) is a sensitive and specific biomarker for sepsis diagnosis, however, the traditional immune-gold labeling detection of PCT is semi-quantitative with relatively low sensitivity (>0.1 ng mL−1). Recently proposed techniques (such as electrochemical immunosensor, microarray, and total internal reflection based methods) require either a complex sensor or sophisticated equipment. Herein, we report a magnetic bead and enzyme-antibody labeled gold nanoparticle based ELISA strategy for sensitive PCT detection. This developed assay is sensitive enough to detect PCT at 20 pg mL−1, efficient for the direct human serum test. Notably, this assay specifically distinguishes PCT from other sepsis markers and the whole assay takes only 1.5 hours to finish.
研究领域:
医学诊断
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免疫学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Liang Huang,Tian Tian,Ying Zhang,Yuting Liao,Jing Wang
Although magnetic beads have been widely utilized as a homogeneous carrier for immunoassays, the effectiveness of the immuno-response is often hindered by the physical and chemical characteristics of these beads, such as the challenge of achieving an optimal equilibrium among magnetic response, suspension performance and colloidal dispersibility. Herein, we present the fabrication of monodisperse polystyrene magnetic beads in a sandwich-like configuration and further explore their immunoassay capabilities as capturing substrates in the detection of procalcitonin (PCT) using the enzyme-linked immunosorbent assay (ELISA). The developed two-step magnetic particles assembly process effectively regulates the magnetic content, while the organic/inorganic double silica layer coating imparts desirable stability and dispersion to the assembly structure. Notably, the prepared magnetic beads exhibit high aqueous dispersity (PDI ∼ 0.2), rapid magnetic separation (25 s) and gradual half-sedimentation time (5 h). Leveraging the magnetic bead-based ELISA, we accomplish not only qualitative screening of PCT at an exceptionally low concentration by naked eye (cut-off value: 0.1 ng/mL) but also highly sensitive quantification of PCT using a microplate reader (limit of detection: 0.031 ng/mL). This investigation offers a solution for striking a harmonious balance among the diverse properties of magnetic beads and demonstrates the perspective of the magnetic capturing substrates in the field of immunoassays.
研究领域:
生物医学工程
·
免疫学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2018
·
IF 1.206
Yun Zhang,Xiaohui Si,Miaomiao Zhang,Xue Yang,Huan Yuan,Xiangpeng Wang,Yang Zhang,Hui Wang
Human procalcitonin is an early diagnostic biomarker for sepsis and bacterial infections and can be used in distinguishing bacterial infections from viral infections. In this study, a colorimetric sensing platform for the rapid determination of procalcitonin was developed. The approach involves the capture of procalcitonin by immunomagnetic beads, and a detection antibody labeled with horseradish peroxidase to perform sandwich format, where it catalyzes the oxidation of 3,3′,5,5′–tetramethylbenzidine to produce the colorimetric signal. Under the optimal conditions, a detection limit of 0.04 ng/mL (3σ) was obtained within the calibration range 0.1–10 ng/mL. The proposed method was performed in less than 90 min and exhibited good specificity without interferences from other biomarkers including C-reactive protein and human serum albumin. Overall, the proposed method provided a new alternative strategy for procalcitonin detection due to its sensitive, rapid, specific, and simple characteristics. This method is suitable for rapid screening of various biomedical targets.
研究领域:
医学诊断
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Juncong Miao,Kang Du,Xuan Li,Xiaoting Xu,Xue Dong,Jinglong Fang,Wei Cao,Qin Wei
Procalcitonin (PCT) as a disease marker is of great significance in the early diagnosis of septicemia and pyemia. Biosensor have good prospects for analysis and detection of disease markers, but developing highly sensitive detection methods for detecting PCT remains a daunting task. In this paper, we develop a ratiometric electrochemical immunosensor with Au NPs modified SiO 2 -Fc-COOH complex as matrix and UiO-66 loaded with Toluidine blue (TB) as a marker for quantitative detection of procalcitonin (PCT). The SiO 2 modified by APTES not only has a large specific surface area but also contains abundant amino groups, which can be connected to the electrochemical probe of ferrocenecarboxylic acid (Fc-COOH). UiO-66 has the advantages of large specific surface area, high porosity and adjustable structure, which can adsorb toluidine blue electrochemical probe through electrostatic attraction . Measurement and analysis of the prepared immunosensor by Differential Pulse Voltammetry (DPV) show that the oxidation peak currents of Fc-COOH and TB appear at potentials of 0.30 V and −0.30 V respectively. As the PCT concentration increases, the oxidation peak current of TB increases and the oxidation peak current of Fc-COOH decreases. The ratios ( ΔI = ΔI TB /ΔI Fc-COOH ) between the double signals could show a certain linear relationship with the concentration of the PCT within a certain range. Under optimal conditions, the linear range of detection obtained by the immunosensor was 1 pg/mL-100 ng/mL and the detection limit was 0.3 pg/mL. In this work, the developed ratiometric electrochemical immunosensor not only provides a simple, reliable and sensitive strategy for quantitative detection of PCT but also provides a useful method for clinical detection of other disease markers.
研究领域:
生物传感器
·
医学检测
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电化学分析
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 9
·2122-2132
·
IF 4.2
Na Wang,Juan Yang,Zhi Luo,Dongmiao Qin,Yusheng Wu,Biyang Deng
A dual-emitting electrochemiluminescence (ECL) immunosensor based on manganese dioxide nanoflowers (MnO2NFs) and zinc sulfide quantum dots (ZnSQDs) was constructed for the first time to sensitively detect procalcitonin (PCT) in human serum. rGO@Ag functioned not only to adsorb primary antibodies (Ab1) but also to improve the electrical conductivity of the immunosensor. The MnO2NFs and ZnSQDs in the nanocomposite, synergistically with silver nanoparticles, simultaneously functioned as cathodic ECL emitters to enhance the detection sensitivity of PCT by shortening the electron-transfer path, thereby reducing energy loss. Under the optimized experimental conditions, the ECL immunosensor was capable of quantitatively detecting PCT in the linear range of 0.1 pg mL−1 to 100 ng mL−1 and over the scanning potential range of −2.0–0 V, with a detection limit of 0.033 pg mL−1. Furthermore, the ECL immunosensor demonstrated high specificity for PCT in the presence of other competing antigens, excellent stability over 10 cycles, and excellent reproducibility, corroborating its potential for measuring PCT concentrations in clinical samples.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2017
·Vol. 35
·期 12
·1861-1868
·
IF 1.852
Lijiang Lei,Yang Zhou,Yuwang Han,Hongman Zhang
Procalcitonin (PCT) is a sensitive and specific biomarker for sepsis diagnosis and widely used as a biomarker to improve the diagnosis of bacterial infections and to guide the antibiotic therapy. In our work, an improved up-converting nanoparticle (UCP) technology based on the immunochromatographic assay (UPT-ICA) was developed for rapid and quantitative detection of PCT. In order to further improve the accuracy, sensitivity and stability of the assay on the basis of our previous study, the UCP coupling with monoclonal antibody of PCT (UCP-Ab1) was freeze-dried under certain conditions. And the detections of PCT levels with UCP-Ab1 conjugates before and after freeze-drying were evaluated. The results show that, compared to the UCP-Ab1 conjugates without freeze-drying, the detection sensitivity of freeze-dried UCP-Ab1 is slightly improved, having a lower immunochromatogragh background and better stability. This improved method can provide a rapid, accurate, and relatively easy way for the clinical detection of PCT.
研究领域:
医学检验
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生物技术
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 23
·6968-6974
·
IF 4.019
Jianqiao Liu,Li Quan,Xuelian Yu,Lin Wang
In this work, a sandwich-type electrochemical immunosensor for the detection of procalcitonin (PCT) is constructed layer-by-layer with a novel label based on MoO3/Au@rGO nanocomposites. The high surface area and electroconductivity of graphene contributes to the transfer of electrons. Besides, the introduction of Au modulates the morphology of the MoO3@rGO nanocomposites from hexagonal prisms to agglomerated particles. Compared to rGO, Au@rGO and MoO3@rGO, the synergistic effect of MoO3/Au@rGO nanocomposites leads to the highest electrocatalytic activity towards H2O2 reduction. The electrochemical immunosensor exhibits a wide working range from 0.01 pg mL−1 to 10 ng mL−1 and a detection limit of 0.002 pg mL−1 at a signal-to-noise ratio of 3. Additionally, we also obtain excellent sensing performance for the immunosensor, including excellent selectivity, good reproducibility and stability. This work will give insight into the application of the nanocomposites based on noble metals combined with transition metal oxides in the fields of electroanalytical chemistry and biosensors.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 105
·103463-103470
·
IF 3.289
Tao Liao,Fang Yuan,Chuan Shi,Chuan-Xin He,Zigang Li
Procalcitonin (PCT) is a potentially specific early marker of bloodstream infection and sepsis. In this work, we report, for the first time, the development of a fluorescence-based immunochromatographic strip test (IST) which employs Eu(DBM)3phen-containing nanoparticles as reporters for the detection of PCT in human serum. Experimental results demonstrate that the developed IST has an excellent ability for rapid (15 min), sensitive (0.05 ng mL−1) and quantitative analysis of PCT, and the assay results are comparable to which of the conventional enzyme-linked immunosorbent assay (ELISA). These results suggest that the developed assay could be utilized for point-of-care detection of PCT.
研究领域:
医学诊断
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Suimin Deng,Yun Hao,Lina Yang,Tongtong Yu,Xiangfeng Wang,Hailing Liu,Yuan Liu,Mengxia Xie
Nanozymes possess the advantages of high stability, adjustable catalytic activity and simple preparation processes, which position them as a promising alternative to natural enzymes. In this work, an oxidase-like nanozyme has been prepared by loading mixed valence manganese oxides (Mn x O y ) on defective PCN-224 MOFs (dPCN). Dodecanoic acid was utilized to introduce abundant mesoporous defects into the dPCN, allowing manganese oxide to grow in situ on the surface and within the pores. The mixed valence state of manganese oxides endowed the Mn x O y @dPCN nanozyme (MdP) with redox and catalytic properties, and the high oxidase-like catalytic performance of MdP for TMB substrate also originated from its favorable electrical conductivity and affinity to the substrate. The reactive oxygen species of the catalytic reaction were mainly singlet oxygen ( 1 O 2 ) and peroxyl radicals (·O 2 − ). Without the existence of hydrogen peroxide (H 2 O 2 ), the nanozyme can rapidly and efficiently oxidize TMB substrate into blue oxidation state, which has strong absorbance at 650 nm. An immunosensor for detecting biomarker procalcitonin (PCT) in human serum samples has been established based on the high catalytic property of MdP nanozyme. The immunoassay for PCT has satisfactory accuracy and repeatability, and its linear detection range can reach to be 0.05–100 ng mL −1 with a limit of detection (LOD) of 0.011 ng mL −1 . The result affords a promising idea to construct oxidase-like nanozyme, and provides a method for sensitive determination of PCT in complex matrices.
研究领域:
生物化学
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医学检测
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomolecules and Biomedicine
·
2025
·
IF 2.2
Tao Qin,Chenxia Rao,Yunsen Deng
Necrotizing enterocolitis (NEC) is a severe, often life-threatening gastrointestinal disease in neonates, predominantly affecting preterm infants, and is frequently complicated by intestinal stenosis—a condition whose nonspecific clinical manifestations make early diagnosis and timely intervention particularly challenging. We aimed to investigate the clinical characteristics of NEC intestinal stenosis and its correlation with the histone demethylase Jumonji domain-containing protein 3 (JMJD3). A total of 310 children with NEC treated between February 2021 and June 2024 were retrospectively enrolled, categorizing them into an NEC group (n=265) and a post-NEC intestinal stenosis group (n=45). General data and laboratory indicators were collected, and analyses were performed to identify factors influencing the development of post-NEC intestinal stenosis. Spearman correlation analysis was utilized to assess relationships between JMJD3 and clinical parameters. Results indicated that the post-NEC intestinal stenosis group exhibited significantly lower platelet counts (PLT) and elevated levels of serum C-reactive protein (CRP), procalcitonin (PCT), and JMJD3 in intestinal tissues (p<0.05). JMJD3 was significantly associated with the development of post-NEC intestinal stenosis (p<0.001), presenting a 3.114-fold increased risk. Furthermore, JMJD3 levels were negatively correlated with PLT levels and positively correlated with CRP and PCT levels (p<0.001). Receiver operating characteristic curve analysis demonstrated that the combination of CRP, PCT, and JMJD3 provided the highest predictive efficiency, with an area under the curve of 0.918, sensitivity of 86.67%, specificity of 86.42%, and a Youden index of 0.731 (p<0.05), all surpassing the performance of individual markers. In conclusion, levels of CRP, PCT, and JMJD3 were significantly elevated in NEC children with intestinal stenosis. Their combined assessment presents a highly effective approach for the early diagnosis of post-NEC intestinal stenosis.
研究领域:
儿科学
·
胃肠病学
·
分子生物学
·
免疫学
·
新生儿学
✓ 已验证
DOI
PubMed
高影响因子
ACS Nano
·
2020
·
IF 14.588
Tianxing Ji,Xinqiang Xu,Xindong Wang,Ning Cao,Xiaorui Han,Minhong Wang,Bo Chen,Zhen Lin,Hongyun Jia,Min Deng,Yong Xia,Xuguang Guo,Meng Lei,Zhenwei Liu,Qiang Zhou,Guanying Chen
Luminescence nanomaterial-based lateral flow assay (LFA) is promising for point-of-care tests. However, the detection sensitivity and accuracy are often affected by the interferences of autofluorescence and photon scattering from nitrocellulose membrane and colored plasma. Here, we describe a near-infrared to near-infrared upconversion nanoparticle (UCNP) immunolabeled LFA for background-free chromatographic detection of sepsis biomarker procalcitonin (PCT) in clinical human plasma. This upconversion immunolabeling enables both light excitation (at ∼980 nm) and anti-Stokes emission (at 800 nm) to be adopted within the first biological window (700–1000 nm), which eliminates background autofluorescence as well as photon scattering interferences, empowering a high-sensitivity detection without complicated procedures. After optimization, the described assay presented a limit of detection reaching down to 0.03 ng/mL, lower than the normal level (0.05 ng/mL), while having a detection range of 0.03–50 ng/mL that covers the clinical PCT level of interest (0.5–10 ng/mL). The assay recoveries in human serum samples were evaluated to be about 95–110%, whereas the inter- and intra-assay coefficient variations were both determined to be below 15%. Importantly, measured PCT concentrations in clinical samples are in good correlation with that of the electrochemiluminescence immunoassay (Roche) widely applied in large clinical settings. This near-infrared to near-infrared upconversion immunolabeling approach has direct implications for ultrasensitive and background-free point-of-care detection of other serum biomarkers in resource-limited clinical settings.
研究领域:
医学诊断
·
生物材料
·
临床检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Yuyun Sui,Aigui Xu,Xiaorui Jin,Jiao Zheng,Xin He,Yan Cheng,Qingji Xie,Rushi Liu
Procalcitonin (PCT) is an important indicator for bacterial inflammatory diseases, and its sensitive, accurate and rapid detection has important clinical value. On the basis of sandwich immunoassay , glucose oxidase-catalyzed gold deposition and in-situ microliter-droplet anodic stripping voltammetry (ASV) of the enzyme-generated gold directly on the immunoelectrode, the ultrasensitive electrochemical detection of PCT is achieved. A new method of the chemical dissolution of gold by an appropriately diluted aqua regia and the simultaneous cathodic preconcentration of gold on the immunoelectrode is suggested, which gives the better performance for the ASV analysis of gold than the reported one. Under optimized conditions, the ASV peak current is linear with the common logarithm of PCT concentration from 0.05 fg mL −1 to 500 ng mL −1 , with a limit of detection (LOD, S / N = 3) as low as 0.04 fg mL −1 . Our method has also been used for detection of PCT in serum samples with satisfactory results.
研究领域:
生物医学工程
·
免疫学
·
临床检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）