FOOD CHEMISTRY
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2021
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IF 7.514
Yu-Xiao Wang,Yue Xin,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
A homogeneous galactoglucan was purified from the alkali-extracted polysaccharides from the basidioma of Macrolepiota albuminosa by gradient ethanol precipitation, whose proposed structure was given for the first time. Results showed it had a molecular weight of 210 kDa, and mainly consisted of glucose and galactose. There were abundant filaments, randomly distributed sheet-like and flaky appearance in its surface by SEM observation. Its backbone comprised β-(1 → 6)-Glc p , α-(1 → 6)-Gal p and β-(1 → 3,6)-Glc p residues at 4:1:1, terminated by β-(1 → 3)-Glc p and T -Glc p residues. Rheological measurements suggested its steady flow behavior was highly dependent on concentrations. Newtonian behavior was evident at low concentrations, whereas pseudoplastic behavior was observed at high concentrations. Besides, the X-ray diffraction patterns proved the presence of amorphous structure. The conformational parameters were detected by HPSEC-MALLS-RI, revealing a random coil conformation in NaNO 3 aqueous solution. This work provides a theoretical basis for the application of polysaccharides from M. albuminosa in food- and drug-based therapies.
研究领域:
食品科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Kangyong Zhang,Sibo Liu,Hongwei Tang,Smith Etareri Evivie,Zengwang Guo,Bailiang Li
Exopolysaccharides (EPS) yield and added concentration of lactic acid bacteria can greatly affect the processing characteristics of fermented milk. In order to investigate the effects and mechanisms of EPS yield and added concentration on fermented milk, researchers extracted EPS from 50 strains of Lactobacillus helvedicus ( L. helvedicus ) and selected the two strains with the largest difference in EPS yield ( L. helvedicus LH18 and L. helvetigus LH33) for subsequent experiments. The physicochemical properties of EPS-LH18 and EPS-LH33 were analyzed. The gel characteristics and protein conformation of fermented milk were studied by means of texture analyzer, rheometer, scanning electron microscopy, nuclear magnetic resonance machine, fluorescence spectrophotometer and circular dichroism . The results indicate that the monosaccharide compositions of EPS-LH18 and EPS-LH33 are the same and have good thermal stability . The texture and rheological properties of L. helveticus LH18 fermented milk are significantly superior to other fermented milk. The reason is that L. helveticus LH18 EPS has the highest yield, which leads to a denser gel structure, lower surface hydrophobicity and free sulfhydryl content of its fermented milk. According to circular dichroism analysis, β- sheet and random coil are the internal factors leading to the difference in fermented milk gel. In addition, the fermented milk improved even more favorably as the concentration of the two EPS additions increased. As described above, L. helveticus LH18 has the potential to be an excellent yogurt starter, and both of the above EPS can be used as probiotic stabilizer alternatives for fermented dairy products.
研究领域:
食品科学
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微生物学
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乳品科学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2023
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IF 5.2
Yulun Chen,Qingrun Liu,Hang Yu,Yahui Guo,Yuliang Cheng,He Qian,Yunfei Xie,Weirong Yao
Titanium dioxide nanoparticles (TiO 2 NPs) could interact with proteins in foods and gastrointestinal fluids, thus changing the properties and functions of proteins. Many research articles have only studied the interactions between TiO 2 NPs and digestive enzymes or the gastrointestinal fate of TiO 2 NPs without considering the effect of food matrix. Therefore, the interactions between TiO 2 NPs and trypsin and its effects on the digestion of collagen in vitro were investigated. Results showed that the functionalization of amino acids (Asp, and Ser) significantly harmed the adsorption of trypsin by TiO 2 NPs. The fluorescence quenching and isothermal titration calorimetry confirmed that the hydrogen bond and van der Waals force played an essential role in the interactions between TiO 2 NPs and trypsin. The β-sheet content of trypsin bound to TiO 2 NPs decreased from 52.40% to 50.73% while the random coil content increased from 20.20% to 22.63%. Binding sites were identified by molecular docking . Additionally, the digestibility of collagen was improved in vitro in the presence of TiO 2 NPs analyzed by SDS-PAGE, hydroxyproline quantification, and the antioxidation of digested products. Hydroxyproline concentration increased by nearly 25% and ABTS radical scavenging rate increased by 50%. This study provides a new insight into the impact of nanoparticles on gastrointestinal digestion.
研究领域:
纳米技术
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生物化学
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食品科学
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胃肠病学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Bilal Muhammad Khan,Hua-Mai Qiu,Xue-Fei Wang,Zhuo-Yan Liu,Jia-Yan Zhang,Yan-Jun Guo,Wei-Zhou Chen,Yang Liu,Kit-Leong Cheong
A complete absence of the physicochemical characterization of Gracilaria chouae polysaccharides (GCP) and its corresponding bioactivities urged the need for this study. It was found that GCP is a heteropolysaccharide which exists in linear random coil conformation. It contained a sulfate content of 7.9% in addition to 52.63% total sugar content and 9.62% galacturonic acid. Galactose and 3,6-anhydrogalactose were found in a molar ratio of 1.0:0.6. Its setting and melting points were determined as 41.3 and 71.7 °C, respectively, which makes it a suitable candidate for industrial processing where further heating is required and/or where the end-product needs to have extended shelf life in hot climate. GCP demonstrated 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis and hydroxyl radical scavenging activity (36.86, 27.42 and 19.07% at 3 mg·ml −1 ). Moreover, the results also suggested its potential use as a prebiotic due to its perceived high fermentability.
研究领域:
海洋生物学
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药学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Zhouyi Xiong,Tingting Weng,Jihui Wang,Shan Xiao,Hao Suo,Dan Li
In this study, the impact of different phosphorylation degrees on ovalbumin (OVA) assemblies was investigated by introducing negatively charged phosphorylated groups as driving forces at pH 9.0 and 85 °C for 2, 4, 6, 10, and 12 h to examine their turbidity, zeta-potential, and average particle size during phosphorylation-mediated aggregation. The presence of phosphate groups in the aggregates was confirmed by 31 P NMR and FTIR spectra. The aggregation process, including amyloid formation, was analyzed by evaluating active thiol groups , intrinsic fluorescence spectra, surface hydrophobicity , circular dichroism , and thioflavin T (THT) fluorescence. TEM and small-angle scattering analysis of the morphological evolution of the self-assembly process over time revealed a structural progression from flexible to longer and thicker flexible fibrils, with the formation of shorter, more rigid semiflexible aggregates after 10h of phosphorylation. When phosphorylated for 12 h, the OVA aggregates exhibited a compact core primarily composed of cross β-structures and surrounded by loosely packed protein segments with random coil structures. Overall, this study highlighted the enhancement of OVA self-assembly by phosphorylation, where electrostatic and hydrophobic interactions play pivotal roles in regulating the formation of protein self-assembly aggregates.
研究领域:
蛋白质化学
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生物物理学
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材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）