TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
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2022
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IF 3.752
Qian WEI,Liu-yang DONG,Cong-ren YANG,Xue-duan LIU,Fen JIAO,Wen-qing QIN
Lime (CaO) and sodium humate (NaHA) were used as the combined depressant for arsenopyrite pre-treated by CuSO 4 and butyl xanthate. Micro-flotation tests show that the combined depressant CaO and NaHA achieved the selective depression of arsenopyrite. Closed-circuit lab-scale test results indicate that the synergistic effect of CaO+NaHA achieved a satisfactory flotation separation of sphalerite and arsenopyrite, for which the Zn grade and recovery of Zn concentrate were 51.21% and 92.21%, respectively. Contact angle measurements, adsorption amount measurements and X-ray photoelectron spectroscopy analysis indicate that the dissolved calcium species (mainly as Ca 2+ ) were adsorbed on the mineral surfaces, thereby promoting NaHA adsorption. Moreover, the surface of the arsenopyrite absorbed more amount of calcium species and NaHA than that of the sphalerite, thereby accounting for the strong hydrophilic surface of arsenopyrite. The adsorption of NaHA on arsenopyrite was mainly chemical adsorption through its carboxyl groups and Ca atoms, whereas that on sphalerite surface was relatively weak.
研究领域:
矿物加工工程
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化学工程
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产品关联
DOI
高影响因子
Chinese Physics B
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2022
·Vol. 31
·期 10
·103302
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IF 1.652
Ya-Wei Zhang,Guan-Hua Ren,Xiao-Qiang Su,Tian-Hua Meng,Guo-Zhong Zhao
Terahertz time-domain spectroscopy (THz-TDS) is an effective nondestructive and noninvasive tool for investigating sulfur-containing pigments. Combined with Raman spectroscopy and vibrational mode analysis, it is significant for artifact identification and conservation. In this work, the terahertz absorption spectra of pararealgar (As4S4) and orpiment (As2S3) samples mixed with polytetrafluoroethylene (PTFE) are characterized in a range from 0.2 THz to 2.2 THz, and their distinctive peaks are observed, respectively. Meanwhile, qualitative analysis is also implemented by using Raman spectroscopy as a complementary technique. The lattice vibrations are simulated by using solid-state density functional theory (ss-DFT), illustrating different characteristic absorption peaks for specific crystalline structures and dynamic properties. This work provides a reliable database of sulfur-containing pigments for using the THz technology to actually analyze and diagnose cultural relics.
研究领域:
文物保护
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材料科学
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化学
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DOI
高影响因子
International Journal of Nanomedicine
·
2023
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IF 8
Yihan Wang,Yizhen Wei,Yichun Wu,Yue Zong,Yingying Song,Shengyan Pu,Wenwen Wu,Yun Zhou,Jun Xie,Haitao Yin
Purpose Realgar, as a kind of traditional mineral Chinese medicine, can inhibit multiple solid tumor growth and serve as an adjuvant drug in cancer therapy. However, the extremely low solubility and poor body absorptive capacity limit its application in clinical medicine. To overcome this therapeutic hurdle, realgar can here be fabricated into a nano-realgar hydrogel with enhanced chemotherapy and radiotherapy (RT) ability. Our objective is to evaluate the superior biocompatibility and anti-tumor activity of nano-realgar hydrogel.Methods We have successfully synthesized nano-realgar quantum dots (QDs) coupling with 6-AN molecules (NRA QDs) and further encapsulated with a pH-sensitive dextran hydrogel carrier with hyaluronic acid coating (DEX-HA gel) to promote bioavailability, eventually forming a multifunctional nano-realgar hydrogel (NRA@DH Gel). To better investigate the tumor therapy efficiency of the NRA@DH Gel, we have established the mice in situ bearing GL261 brain glioblastoma as animal models assigned to receive intratumor injection of NRA@DH Gel.Results The designed NRA@DH Gel as an antitumor drug can not only exert the prominent chemotherapy effect but also as a “sustainable reactive oxygen species (ROS) generator” can inhibit in the pentose phosphate pathway (PPP) metabolism and reduce the production of nicotinamide adenine dinucleotide phosphate (NADPH), thereby inhibiting the conversion of glutathione disulfide (GSSG) to glutathione (GSH), reducing GSH concentrations in tumor cells, triggering the accumulation of ROS, and finally enhancing the effectiveness of RT.Conclusion Through the synergistic effect of chemotherapy and RT, NRA@DH Gel effectively inhibited the proliferation and migration of tumor cells, suppressed tumor growth, improved motor coordination, and prolonged survival in tumor-bearing mice. Our work aims to improve the NRA@DH Gel-mediated synergistic chemotherapy and RT will endow a “promising future” for the old drug in clinically comprehensive applications.
研究领域:
药学
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肿瘤学
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纳米技术
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生物医学工程
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
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IF 5.4
Jianchao Li,Yuanjing Ma,Nuo Deng,Fei Wang,Yi Tao,Huilin Zhu,Weichen Xu,Jinjun Shan,Yongming Li,Xiao Wu
Ethnopharmacological relevance Realgar is a traditional Chinese medicine rich in arsenic, and long-term use may lead to arsenic poisoning. The toxicity of realgar has primarily focused on the liver and kidney, with limited studies on its intestinal toxicity. Niuhuang Jiedu Tablet (NHJDT) is a renowned realgar-containing formula for clearing heat and detoxifying. NHJDT compatibility significantly reduces the toxicity of realgar, but the detoxification effects and underlying mechanisms in reducing realgar-induced intestinal toxicity remain unclear. Aim of the study The study aims to investigate the detoxification mechanism of NHJDT's compatibility in reducing the intestinal toxicity of realgar. Materials and methods To evaluate the intestinal detoxification efficacy of NHJDT, mice were orally administered 0.5 % CMC-Na, realgar (0.5 and 1.0 g/kg) or NHJDT (3.9 and 7.8 g/kg) for 14 days. The intestinal detoxification effects of NHJDT were evaluated by toxic symptoms and intestinal barrier function. Changes of body weight, colon length, histopathological damage, tight junction and MUC2 proteins, and goblet cells were determined. The inflammatory factors and DAO were detected by ELISA. Further investigations including 16S rRNA sequencing, untargeted metabolomics, targeted short-chain fatty acid metabolomics and molecular docking assays were performed to explore the underlying detoxification mechanisms of NHJDT. Additionally, targeted microbiota and its derived metabolite gavage were conducted in mice to verify the detoxification effects. Results NHJDT's compatibility significantly mitigated realgar-induced intestinal toxicity and gut barrier damage. It restored gut microbiota homeostasis by modulating the abundance of Akkermansia and concurrently enhanced the transformation from tryptophan to indole-3- lactate (ILA). The supplementation with Akkermansia muciniphila ( A. muciniphila ) and its derived ILA activated the aryl hydrocarbon receptor (AhR) in mice, thereby ameliorating realgar-induced intestinal damage. Conclusion NHJDT's compatibility alleviated realgar-induced intestinal toxicity by activating the AhR pathway via A. muciniphila and ILA. This study elucidates the detoxification mechanism of NHJDT's compatibility and offers novel insights for the clinical treatment of arsenic poisoning.
研究领域:
药理学
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微生物学
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毒理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
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2022
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IF 2.65
Mingyi Sun,Yaolei Li,Ying Wang,Huimin Wu,Jing Liu,Longtai You,HuLinyue Peng,Huating Huang,Hongyu Jin,Xiaoxu Dong,Changhai Qu,Xingbin Yin,Jian Ni
At present, several experiments have been carried out to study the changes in total arsenic content of realgar and its prescription, but few researches on its form and valence. We evaluated the change in arsenic species concentration in realgar from the perspective of absorption by using an in vitro dissolution study, an in vivo unidirectional intestinal perfusion study, transmembrane transport in Caco-2 cells, and a pharmacokinetic study in rats. In the gastrointestinal tract, arsenic species are mainly present inorganic forms of AsIII and AsV. The cumulative dissolution rates of soluble arsenic in 4 h artificial gastric fluid and 8 h artificial intestinal fluid were 21.99% and 59.20%, respectively. The values of soluble arsenic in realgar in the duodenum, jejunum, and ileum of rats were 5.4 × 10−3, 6.1 × 10−3 and 5.8 × 10−3 cm/min, respectively. In the process of small intestine perfusion, the AsIII of realgar was partially converted into AsV in the duodenum and jejunum. As the transport time increased, the transmembrane transport rate and value of soluble arsenic in realgar were increased in Caco-2 cells, and it also suggested that arsenic species may be passively transported across the Caco-2 cell monolayer. The Cmax and AUC (0-24) of AsIII, AsV, and DMA in plasma of realgar were 41.26 ng L−1/343.977 ng h mL−1, 21.626 ng L−1/47.310 ng h mL−1, and 2.372 ng L−1/30.429 ng h mL−1, respectively. Tmax and MRT (0-∞) of AsIII, AsV, and DMA were 2.571 h/9.649 h, 0.393 h/2.790 h, and 3.143 h/23.145 h, respectively. It is hoped to provide a basis for clarifying the arsenic species in realgar.
研究领域:
药学
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毒理学
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药理学
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产品关联
DOI
PubMed
高影响因子
Journal of Nanomaterials
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2023
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IF 3.791
Shengyan Pu,Wenwen Wu,Dongsheng Shi,Yanqi Dai,Jun Zhang,Mingyan Zhao,Shuangyu Liu,Guihong Zheng,Xingqi Wang,Yu Yan,Jun Xie
Realgar, a traditional Chinese medicine, has notable antitumor activity and can be used in various hematologic tumor and solid tumor therapies in a wide range of clinical applications. Nanosized realgar possesses higher surface activity and enhanced therapeutic effects on cancer cells compared with those of bulk realgar. In this study, we reported the design of high-performance nanorealgar quantum dots (NR QDs) with aggregation-induced emission enhancement of crystal units (CUs) with the addition of hydrochloric acid. The enhanced fluorescence characteristics of NR QDs are associated with an optimized crystal structure obtained by accelerating the growth process of NR CUs in which the smaller NR CUs facilitate a self-assembling arrangement and a complete structural optimization process, ultimately forming regular crystallization with a size quantization effect. To improve the theranostic efficiency of NR QDs, we synthesized NR QDs coupled with a tripeptide of arginine–glycine–aspartic acid (RGD) with tumor neovascular targeting. We intravenously injected [email protected] QDs into BALB/c mice bearing a subcutaneously transplanted breast tumor (4T1). Our results indicate that functional [email protected] QDs are effective chemotherapeutic drugs that can anchor into the tumor endothelial cells through an active targeting effect, inhibit the angiogenesis in the tumor, and eventually “cut-off” the nutrient-rich blood supply to the tumor. This promising antiangiogenesis therapeutic strategy induces [email protected] QDs to penetrate tumor-fenestrated vascular networks and has potential clinical value.
研究领域:
纳米技术
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癌症研究
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药学
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生物医学工程
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分子生物学
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产品关联
DOI
高影响因子
Scientific Reports
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2016
·Vol. 6
·期 1
·1-12
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IF 5.228
Ma Qiang,Wang Chuan,Li Xiaojin,Guo Hua,Meng Jie,Liu Jian,Xu Haiyan
Realgar (As 4 S 4 ) has been demonstrated to be effective for the treatment of acute myeloid leukemia (AML); it has the advantages of no drug resistance and oral administration. Nevertheless, its poor solubility has been an obstacle to its bioavailability, requiring high-dose administration over a long period. We investigated whether crushing realgar crystals to the nanoscale and encapsulating the particles in a water-soluble polymer in one step using hot-melt extrusion would increase the bioavailability of As 4 S 4 . Raw As 4 S 4 (r-As 4 S 4 ) and water-soluble polymer were processed via co-rotating twin screw extrusion. The resulting product (e-As 4 S 4 ) was characterized by SEM, XRD, and DLS. The cytotoxicity and therapeutic effects of e-As 4 S 4 were evaluated in vivo and in vitro . The results show that e-As 4 S 4 dissolved rapidly in water, forming a stable colloid solution. The average size of e-As 4 S 4 particles was 680 nm, which was reduced by more than 40-fold compared with that of r-As 4 S 4 . The bioavailability of e-As 4 S 4 was up to 12.6-fold higher than that of r-As 4 S 4 , and it inhibited the proliferation of HL-60 cells much more effectively than did r-As 4 S 4 , inducing apoptosis and significantly reducing the infiltration of HL-60 cells into the bone marrow, spleen, and liver. This in turn prolonged the survival of AML mice.
研究领域:
药理学
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肿瘤学
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材料科学
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治疗学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2025
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IF 4.8
Donghan Bai,Zihao Zhang,Jingyue Gao,Qiaochu Wang,Remy Macdonald,Ziwen Xu,Shumin Chen,Nanxi Huang,Lu Luo
Background: Leukemia is a malignant hematologic disease that poses a serious threat to human health. Realgar-Indigo Naturalis formula (RIF), a traditional Chinese medicine formula, have demonstrated anti-tumor activity, but its mechanisms of action remain unclear.Objective: This study aimed to establish a zebrafish HL-60 tumor transplantation model to investigate the anti-leukemic effects of RIF and explore its underlying mechanisms.Methods: The zebrafish HL-60 tumor transplantation model was established, with RIF as the intervention drug and all-trans retinoic acid (ATRA) as the control. Anti-tumor efficacy was assessed via pharmacodynamic analysis. Transcriptomic analysis further elucidated gene expression profiles, differentially expressed genes, and relevant biological pathways.Results: RIF significantly reduced tumor cell fluorescence intensity, demonstrating anti-tumor efficacy. Additionally, it improved HL-60-induced liver damage in zebrafish, as evidenced by a reduction in fatty vacuolar degeneration in liver tissue. Transcriptomic analysis revealed that ATRA mainly affected the FoxO signaling pathway, PI3K-Akt signaling pathway, apoptosis, and complement and coagulation cascades in leukemia treatment. RIF primarily influenced the ubiquitin-proteasome system, ferroptosis, and glutathione metabolism. The combination of ATRA and RIF also affected autophagosome and lysosome pathways, in addition to the aforementioned pathways.Conclusion: RIF exhibit significant anti-tumor effects through modulation of multiple pathways. This study provides a theoretical foundation for the potential clinical application of RIF in leukemia treatment.
研究领域:
肿瘤学
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药理学
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中医学
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发育生物学
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毒理学
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基因组学
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产品关联
DOI
PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
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2021
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IF 2.63
Songsong Wang,Xiao Xiao,Ao Li,Peng Li
An-Gong-Niu-Huang Wan (AGNH) has been a well-known cinnabar- and realgar-containing compound recipe for cerebral diseases. Unfortunately, its clinical practice is often restrained by the specific hepatorenal toxicity of cinnabar and realgar (C + R). In previous research studies, we have found that the antioxidative and anti-inflammatory effects of its herbal constituents could mitigate the risks from the toxicity. The underlying detoxification mechanisms are still unsolved. The present study investigated the protective effects of AGNH’s herbal constituents on hepatorenal injury induced by C + R. For the mice treated with C + R, the increased expression levels of sensitive biomarkers of metal exposure and hepatorenal toxicity, including metallothionein (MT) in both hepatorenal tissues and kidney induced molecule-1 (KIM-1) in the kidney, were simultaneously reduced when C + R coadministered with other herbal medicines. In addition, the contents of trivalent As (AsIII), pentavalent As (Asv), and mercury (Hg) in hepatorenal tissues of mice were also significantly reduced benefiting from the herbal constituents in AGNH. Further mechanism studies showed that the herbal constituents in AGNH could downregulate the expressions of uptake transporters (AQP9 and OAT1) and upregulate the expressions of efflux transporters (P-gp, MRP2, and MRP4) in mice intoxicated by C + R. Our results suggested that AGNH’s herbal constituents protect the body against C + R-induced hepatorenal toxicity and accumulations of Hg and As, which could be associated with the reestablishment of heavy metal homeostasis and the detoxification system.
研究领域:
药理学
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毒理学
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分子生物学
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药学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2022
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IF 6.14
Dongsheng Shi,Shengyan Pu,Haitao Yin,Yingying Song,Hongmei Liu,Xiaolian Yu,Yang Zhao,Chengkun Ye,Shuangyu Liu,Xingqi Wang,Jinjin Huang,Yu Zhang,Jun Xie
Realgar as a traditional Chinese medicine for curing cancer has unique advantages, such as great antitumor effect, low genotoxicity, and good organism tolerability. Unfortunately, its poor solubility and body absorptive capacity may cause an extremely low bioavailability. To overcome the therapeutic hurdle, we have successfully synthesized high-performance fluorescent realgar nanoclusters (RNCs), which encapsulate “ethanolamine-modified nano-realgar (NR) assemblies” into functional a poly(ethylene glycol) (PEG)–phospholipid hydrophobic layer, simultaneously coupling with a targeting ligand of hyaluronic acid (HA). As a chemotherapeutic agent, the designed HA-modified RNCs (RNCs@HA) possess excellent water dispersbility, pH-sensitive NR quantum dots (QDs) release capacity, low toxicity, and enhanced anticancer efficacy. As a fluorescent probe, RNCs@HA can be used as an in vivo fluorescence trace system to measure and image biological activity. Herein, we have established a BALB/c mice model bearing a subcutaneous transplanted breast tumor and designed the intravenous RNCs@HA-mediated tumor theranostics in vivo. Under the guidance of targeting HA ligands, RNCs@HA concentrates in the tumor tissue from fenestrated neovascularization, gradually degrades into hydrophobic NR QDs, gathers around nucleopores, and moves into the nucleus regions of cancer cells in an acidic environment, conclusively facilitating nuclear targeting-associated chemotherapy. Our work aims to improve the chemotherapeutic efficacy of realgar and facilitate its more widespread use for clinical application.
研究领域:
药学
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纳米技术
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癌症研究
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
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2021
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IF 9.776
Shengmei Wang,Xuanjun Liu,Shengfeng Wang,Linqi Ouyang,Hui Li,Jinsong Ding,Guiming Deng,Wenhu Zhou
Discovery of BCR-ABL1 tyrosine kinase inhibitors (TKIs) has revolutionized the therapy of chronic myeloid leukemia (CML), a malignant myeloproliferative disease characterized by abnormal activation of BCR-ABL fusion oncoprotein with protein tyrosine kinase (PTK) activity. However, the long-term treatment outcomes with TKIs are strongly limited by multiple drug resistances, resulting in relapse albeit with initial high response rate. Here, we reported a realgar (As 4 S 4 ) nanocrystal-based delivery system to reverse drug resistance for synergistic CML therapy. While As 4 S 4 is extremely insoluble in water, bovine serum albumin (BSA) was rationally screened to effectively stabilize As 4 S 4 nanocrystal with uniformed size of ~40 nm. Imatinib (IMA), a representative TKIs, can be readily loaded into the hydrophobic domain of BSA to develop As 4 S 4 /IMA co-delivery system. Mechanistically, IMA inhibits PTK activity, while As 4 S 4 degrades BCR-ABL1, which co-contribute to tumor suppression via complementary pathways for synergistic effect. Moreover, the nanosystem was modified with folic acid (FA) to enable tumor targetability, which has been demonstrated both in vitro and in vivo, resulting in robust tumor growth inhibition and significantly prolonged mice survival without any noticeable adverse effects. This work designed a synergistic nanoplatform for targeted CML therapy, provided a strategy to address the key limitation of As 4 S 4 for biomedical applications, and highlighted the advantages of the combination between traditional Chinese and western medicine for diseases treatment.
研究领域:
肿瘤学
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药学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）