JOURNAL OF ETHNOPHARMACOLOGY
·
2019
·
IF 3.414
Nur Amanina Syariff Tan,Nelli Giribabu,Kamarulzaman Karim,Shaik Nyamathulla,Naguib Salleh
Ethnopharmacological relevance Marantodes pumilum (MP) (Kacip Fatimah) is used to maintain the well-being of post-menopausal women. However, its role in ameliorating post menopause-related vaginal atrophy (VA) is unknown. Aims To investigate the ability of intravaginal MP gel treatment to ameliorate VA in sex-steroid deficient condition, mimicking post-menopause. Methods Ovariectomized female Sprague-Dawley rats received MP (100 μg/ml, 250 μg/ml and 500 μg/ml) and estriol (E) gels intravaginally for seven consecutive days. Rats were then euthanized and vagina was harvested and subjected for histological and protein expression and distribution analyses. Vaginal ultrastructure was observed by transmission electron microscopy (TEM). Results Thickness of vaginal epithelium increased with increasing intravaginal MP doses. Additionally, increased in expression and distribution of proliferative protein i.e. PCNA, tight junction protein i.e. occludin , water channel proteins i.e. AQP-1 and AQP-2 and proton extruder protein i.e. V-ATPase A1 were observed in the vagina following intravaginal MP and E gels treatment. Intravaginal MP and E gels also induced desmosome formation and approximation of the intercellular spaces between the vaginal epithelium. Conclusions Intravaginal MP was able to ameliorate features associated with VA; thus, it has potential to be used as an agent to treat this condition.
研究领域:
药理学
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妇科学
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分子生物学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Yiqun Chen,Mengyu Li,Yang Tong,Zizheng Liu,Liping Fang,Yi Wu,Zheng Fang,Feng Wu,Li-Zhi Huang
We report a process using NiFe 2 O 4 nanocatalyst for the heterogeneous activation of sulfite ions for estriol (E3) oxidation. This NiFe 2 O 4 -sulfite system consisted of NiFe 2 O 4 as an catalyst, sulfite as a reductant, O 2 as an oxidant, and oxysulfur radicals (SO 3 − , SO 4 − and SO 5 − ) and hydroxyl radicals (HO ) as generated radicals. The experimental conditions for E3 removal were investigated, including the initial pH values, dissolved oxygen concentration, and NiFe 2 O 4 and sulfite ion dosages. The initial pH has a significant influence on the catalytic performance, and approximately 87.6% of E3 could be removed at pH 9.0. NiFe 2 O 4 nanocatalyst was characterized by high resolution and field emission transmission electron microscopy, X-ray diffractometry, and X-ray photoelectron spectroscopy. The catalytic reactivity and stability of the NiFe 2 O 4 nanocatalyst were investigated by consecutive repeated runs. Radicals generated in this NiFe 2 O 4 -sulfite system were identified via radical-scavenging and electron spin resonance experiments. SO 5 − was identified as major oxidative radicals for E3 removal, which is quite different from the previous studies on sulfite activation . The generation of >SO 3 2− -Ni(II)-OH on NiFe 2 O 4 surface played a critical role in sulfite auto-oxidation. Our results supplemented the mechanism of the sulfate radical generation with sulfite ion as a source.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 7
·1109-1119
·
IF 2.613
Yongjuan Meng,Jinling Pu,Jiamei Gan,Jianping Li
A composite of the metal–organic framework compound ZIF-8 doped with CdSe quantum dots (QDs) with sensitive and stable luminescence was synthesized, and a molecularly imprinted electrochemiluminescence (ECL) sensor was constructed based on this composite. The ZIF-8@CdSe molecularly imprinted ECL sensor combines the high sensitivity of ECL and the high selectivity of molecular imprinting to realize the sensitive and specific detection of estriol. CdSe QDs and gold nanoparticles were encapsulated within ZIF-8 to obtain the ZIF-8@CdSe QDs/GNP (ZIF@CdSe/GNP) composite. Subsequently, the GNPs were further loaded on the surface of this composite to obtain the GNP/ZIF@CdSe/GNP composite. l -Cysteine was used to immobilize the GNP/ZIF@CdSe/GNP composite on the surface of a gold electrode to obtain the GNP/ZIF@CdSe/GNP-modified gold electrode. A molecularly imprinted polymer (MIP) film was prepared on the surface of the modified electrode by electropolymerization with o -phenylenediamine as the functional monomer and estriol as the template molecule. After elution, estriol could be specifically recognized by the cavities. The readsorption of estriol by the MIP can prevent the coreactant from reaching the electrode surface through the cavities, thereby weakening ECL. A good linear relationship existed between the ∆ECL and lg C of estriol concentrations of 1 × 10 −14 to 1 × 10 −9 mol·L −1 . The detection limit was as low as 8.9 × 10 −16 mol·L −1 . The sensor was applied in the determination of estriol in serum samples with a recovery of 97.0–102%.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Qiaoying Wu,Xuechi Yin,Yuanyuan Cheng,Chaoying Wang,Jiaqi Ma,Qingzhe Zhang,Huihui Liu,Ahmed Youssef,Jianlong Wang,Daohong Zhang
Excessive intake of estrogen poses significant health risks to the human body; hence, there is a necessity to develop rapid detection methods to monitor its levels of addition. Gold nanoparticles (AuNPs), commonly utilized as colorimetric signal labels, find extensive application in lateral flow immunoassay (LFIA). However, the detection sensitivity of traditional AuNPs-LFIA is typically constrained by low molar extinction coefficients and reliance on a single signal. Herein, in this work, unique spark-type AuCuPt nanoflowers modified with tannic acid (AuCuPt@TA) were precisely designed by reasonable layer-by-layer element composition and green modification. The obtained AuCuPt displays robust broadband absorption spanning the visible to near-infrared spectrum, showcasing a notable molar extinction coefficient of 2.38 × 1012 M–1 cm–1 and a photothermal conversion efficiency of 48.5%. Based on this, selecting estriol (E3) as a model analyte, colorimetric/photothermal dual-signal LFIA (CLFIA and PLFIA) was developed. Limits of detection (LOD) of the CLFIA and PLFIA were achieved at 0.033 ng mL–1 and 0.021 ng mL–1, respectively, which represent a 9.3- and 14.6-fold improvement compared to the visual LOD of AuNPs-LFIA. Moreover, the application feasibility of the immunoassay was further evaluated in the milk and pork with satisfactory recoveries ranging from 86.21% to 117.91%. Thus, this work has enhanced the performance of LFIA for E3 detection and exhibited enormous potential for other sensing platform construction.
研究领域:
生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Feilong Dong,Cong Li,Xingmao Ma,Qiufeng Lin,Guilin He,Shipeng Chu
Estriol (E3) is a potent intracellular estrogen and endocrine disruptor that has been frequently detected in surface water in recent years. We investigated the degradation kinetics of E3 during chlorination in a pilot-scale water distribution system (WDS), and found that its degradation followed second-order kinetics. Higher concentrations of bromide and neutral acids promoted the degradation of E3. In addition, the degradation of E3 was higher at higher water velocity in WDS. This study further compared the degradation of E3 in different pipe materials and the results indicated that the degradation of E3 was most efficient in stainless-steel pipe, followed by the polyethylene pipe and then ductile iron pipe. To gain more insights into the mechanisms of E3 degradation, intermediate products from E3 degradation were analyzed. Five intermediates were identified, and a possible pathway was proposed by combining the experimental observations with the density functional theory (DFT) calculation. Four types of disinfection byproducts (DBPs) in ductile iron pipe were detected from E3 degradation during chlorination, confirming that DBPs are generated by E3 during the free chlorine oxidation, which pose a greater safety threat to drinking water.
研究领域:
环境科学
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生物学
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化学工程
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产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
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2019
·
IF 2.398
Lipeng Yuan,Bo Liu,Kaidan Yin,Zhen-Lin Xu
In this study, a heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of estriol (E3) in milk was developed. Rabbit polyclonal antibody specific for E3 was raised by introducing a carboxyl spacer arm from bromoacetic acid of E3 as immunizing hapten. A heterologous ciELISA using a coating hapten containing a carboxyl spacer arm from 6-ketoestriol of E3. The assay exhibited the required sensitivity for detection of E3 with limit of detection of 0.07 ng mL−1 and a working range from 0.41 ng mL−1 to 15.78 ng mL−1. Good recoveries were obtained ranging from 83.2% to 125.8% for E3 spiked milk samples. A satisfactory correlation (R2 = 0.994) between the results of ciELISA analysis and that of LC–MS/MS analysis indicated good accuracy and reproducibility of the proposed method, which was ideally suited as a rapid screening tool for routine E3 monitoring.
研究领域:
生物化学
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分析化学
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食品科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
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2023
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IF 8.3
Longjie Liu,Zeyang Lu,Wanling Cai,Gary Owens,Zuliang Chen
Reduced graphene oxide/iron nanoparticles (rGO/FeNPs) synthesized by the chemical method have been used in Fenton oxidation of organic contaminants , yet little is known about biosynthesized rGO/FeNPs using green tea extract (GT) as how to activate persulfate in sulfate radical-based advanced oxidation processes . In this study, rGO/FeNPs were used to activate peroxydisulfate (PDS) for 17β-estradiol (βE 2 ) and estriol (E 3 ) removal. The rGO/FeNPs-PDS system removed 83.6% of βE 2 and 62.5% of E 3 within 240 min, which was confirmed by a combination of adsorption and degradation via both radical and non-radical pathways. Four main reactive species in βE 2 and E 3 degradation were observed, i.e., hydroxyl radical (·OH), sulfate radical (SO 4 ·− ), singlet oxygen ( 1 O 2 ) and electron transfer , with the respective contributions of ·OH (32.9 and 34.7%), SO 4 ·− (16.1 and 19.7%), 1 O 2 (12.2 and 14.1%) and electron transfer (8.0 and 7.2%). Analysis of X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR), Electron Paramagnetic Resonance (EPR) and electrochemical measurements all indicated that beside the well-known role of Fe, C O from rGO through the generation of ·OH, SO 4 ·− , 1 O 2 and electron transfer, as well as GT through electron transfer also participated in the activation of PDS. Finally, the degradation pathways of βE 2 /E 3 were proposed. Overall, this study provides a new insight into the biosynthesis of rGO/FeNPs to activate PDS for the oxidation of mixed emerging contaminants .
研究领域:
环境科学
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化学工程
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生物学
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产品关联
DOI
PubMed
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2022
·Vol. 36
·期 17
·e9345
·
IF 2.586
Chong-Xin Kang,Ru-Ting Fan,Hua-Ming Xiao,Xian Wang
Rationale Assessing estrogen concentrations in biological systems can provide valuable information on physiological processes, which is crucial for the early diagnosis of many diseases. Because estrogens are present in the human body in low concentrations and in a wide dynamic range, analytical methods with high sensitivity and specificity are required for their determination in complex biological matrices. Methods To discover an appropriate derivatization reagent for estrogen mass spectrometry (MS) analysis, we compared five sulfonyl chloride derivatization reagents, namely 3-methyl-8-quinolinesulfonyl chloride (MQSCl) and 8-quinolinesulfonyl chloride (QSCl), 1-methyl-1 H -pyrazole-4-sulfonyl chloride, 1,2-methyl-imidazole-5-sulfonyl chloride, and dansyl chloride. By selecting the derivatization reagent with the best performance, we developed and validated a novel chemical derivatization–assisted-liquid chromatography-electrospray ionization-tandem mass spectrometry (CD-LC-ESI-MS/MS) method to simultaneously determine the concentrations of estrone, estradiol, and estriol (E1, E2, and E3) in human serum. Results It was found that among the five investigated reagents, MQSCl-derivatized estrogens presented the highest sensitivity using LC-ESI-MS/MS. Based on this discovery, MQSCl was chosen to derivatize the analyzed estrogens to assist LC-ESI-MS/MS analysis. The limit of quantification of E1, E2, and E3 was measured as 2.7, 4.6, and 5.1 pg/mL, respectively. Inter- and intra-day precision, expressed as the coefficient of variation, was shown to be lower than 13.2% for all concentrations. The mean recovery was 72.4% overall, with good reproducibility at low, medium, and high concentrations in the calibration range. Conclusions The developed method was successfully applied to the quantitative determination of estrogens in clinical human serum from pediatric and adult women, demonstrating the suitability of estrogen analysis in the biological matrix at low concentration (pg/mL).
研究领域:
分析化学
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临床化学
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质谱分析
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DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2021
·
IF 4.384
Lin Zhu,Xiang Yu,Qian Li,Yuanyuan Zhang,Yiguang Jin,Lina Du
Estriol can be used to treat radiation-induced leukopenia by increasing peripheral blood leukocytes and therefore it plays an important role in radiation protection. However, only high-dose injectable suspensions are available when estriol is used to combat against ionizing radiation-induced injury. Intramuscular (i.m.) administration of estriol is very painful and inconvenient, and the lack of timely self-administered formulation greatly limits the wide application of estriol. This will facilitate quick response under emergent conditions in complementary with the available estriol formulations. Herein, we prepared estriol microneedle (MNs) patches for the convenient and efficient treatment of radiation-induced injury. A biocompatible polymer, polyvinylpyrrolidone K90, was dissolved in an estriol solution of methanol and cast into a mold to obtain conical-shaped MNs. N-vinyl pyrrolidone was poured on the base of the MNs and photocured to enhance the mechanical strength of estriol MNs (EMNs). EMNs were easily pierced 200 μm into the mouse skin. More importantly, the EMNs tips were dissolved very quickly within 5 min so that the drugs could permeate across skin. Mouse models of ionizing radiation-induced injury were established with 6.5 Gy radiation of 60 Co γ ray. Moreover, EMNs increased peripheral blood leukocytes in irradiated mice, protected the bone marrow hematopoietic system, and improved the survival rate of the irradiated mice to 80%. EMNs are a promising transdermal drug delivery system that allows for easy, rapid administration and protects the body from damage caused by ionizing radiation.
研究领域:
药学
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辐射生物学
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药物递送系统
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2023
·Vol. 15
·期 5
·2625-2635
·
IF 3.2
Luo Xiaoliang,Zhao Bincheng,Yao Junwen,Peng Mingguo,Mao Linqiang,Zhang Wenyi,Shen Rongyan
A large amount of estrogen is introduced into the feed to improve livestock growth, excessive residual estrogen would exist in the excrements. The accumulation of estrogen can cause the water and soil pollution around the farm, further inhibit growth and induce caner for organisms and human. Anaerobic digestion (AD) could effectively remove estrogen in livestock manure while the mechanism is still unclear. In this study, the mechanism of estrogen removal by AD was explored during AD process of pig manure. Estrone (E1), estradiol (E2) estriol (E3) and ethinylestradiol (EE2) were selected as research subjects. The removal rates of E1, E2, E3 and EE2 were 19.14, 28.62, 25.83 and 11.81%. Dissolved organic matters (DOM), especially humic acid, play an important role in reducing bioavailability of estrogen. Estrogen will be absorbed by DOM through structures such as aromatic ring and amides. Estrogen may also promote the growth of microorganisms which could degrade estrogen, such as Rhodococcus , Sphingomonas and Pseudomonas . The amount of these microorganisms and dissolved microbial metabolites did not change obviously. This study would give a new explanation for the removal of estrogen and can provide theoretical support for harmless treatment of pig manure. Graphical
研究领域:
环境科学
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微生物学
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生物技术
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生态毒理学
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废弃物管理
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Qian Zhao,Yousef Faraj,Lu-Yue Liu,Wei Wang,Rui Xie,Zhuang Liu,Xiao-Jie Ju,Jie Wei,Liang-Yin Chu
A facile and efficient electrochemical biosensing platform based on screen printed carbon electrode (SPCE) modified with three-dimensional (3D) nanocomposite consists of reduced graphene oxide (RGO) with the insertion of silver nanowires (AgNWs) followed by the anchoring of silver nanoparticles (AgNPs) is constructed as RGO/AgNWs/AgNPs/SPCE for the simultaneous determination of dopamine (DA), uric acid (UA) and estriol (EST). The morphology characteristic and surface elemental composition of RGO/AgNWs/AgNPs nanocomposite are investigated by field-emission scanning electron microscope, transmission electron microscope and X-ray photoelectron spectroscope. Cyclic voltammetry, electrochemical impedance spectroscopy, linear sweep voltammetry and differential pulse voltammetry are utilized to explore the electrochemical performances of the constructed electrodes. Due to abundant active sites and excellent electrocatalytic activity of the nanocomposite, the RGO/AgNWs/AgNPs/SPCE sensor exhibits well-resolved oxidation peaks and enhanced oxidation peak currents in the ternary mixture of DA, UA and EST with respective linear response ranges of 0.6 to 50 μM, 1 to 100 μM and 1 to 90 μM and detection limits (S/N = 3) of 0.16 μM, 0.58 μM and 0.58 μM, respectively. Moreover, the constructed biosensor exhibits good selectivity, reproducibility and stability, and excellent performance in determining DA, UA and EST in synthetic urine samples with excellent recovery. The results reveal that the RGO/AgNWs/AgNPs nanocomposite is a promising candidate for advanced electrode material in electrochemical sensing field and possesses great application prospects in further sensing researches.
研究领域:
分析化学
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材料科学
·
纳米技术
·
电化学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Jiashun Cao,Boming Fu,Teng Zhang,Yang Wu,Ziyan Zhou,Jianan Zhao,E Yang,Tangjian Qian,Jingyang Luo
In this study, the distribution, removal efficiency, and potential risks of 9 typical endocrine active compounds (EACs) in two full-scale wastewater treatment plants (WWTPs) were investigated. The EAC concentrations ranged from 0.2 to 7394.2 ng/L in influents. The source of influents was a critical factor in determining the EAC levels. EACs were primarily removed in the secondary biological processing units, with removal efficiencies fluctuating from 13.7% to 98%. The biological treatment processes and operating parameters ( i.e., HRT and SRT) influenced the EAC removal efficiency. Bisphenol A (BPA) and estriol were mainly removed by biodegradation, while antidepressants were primarily eliminated by sludge adsorption according to the distribution patterns and mass flow of EACs in WWTPs . Novosphingobium, Saprospiraceae, etc. were the core functional bacteria for EAC biodegradation. In addition, sertraline in effluents and dewatered sludge may pose medium environmental risks, while the other EACs pose low environmental risks.
研究领域:
环境科学
·
水处理
·
毒理学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）