JOURNAL OF MICROBIOLOGY
·
2013
·Vol. 51
·期 2
·166-173
·
IF 1.276
Wu Qiong,Bai Linquan,Liu Weicheng,Li Yingying,Lu Caige,Li Yaqian,Fu Kehe,Yu Chuanjin,Chen Jie
Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea . To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea , this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01- chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01- chit42 CT on B. cinerea , including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma , which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.
研究领域:
微生物学
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植物病理学
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基因工程
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Junwei Huang,Zilin Zhu,Ruomu Chen,Dandan Pan,Qing X. Li,Xiangwei Wu
Ibuprofen, a widely used nonsteroidal anti-inflammatory drug, contaminates agricultural products and potentially threatens human health due to its frequent detection and poor biodegradability. Microbial metabolism dominates the elimination of residual ibuprofen in the environment. In mineral salt medium at pH 6 with 5 mM glucose, Streptomyces sp. D218 transformed ibuprofen concentrations ranging from 0.05 to 0.40 mM in 24 h. The optimal temperature, pH, and initial OD600 nm for ibuprofen transformation by strain D218 were 25–37 °C, 5.0–6.0, and 1.0–1.5, respectively. Strain D218 could simultaneously transform ibuprofen into the intermediates 2-hydroxyibuprofen and ibuprofen amide (IBUA). The two intermediates were further metabolized to 2-hydroxyibuprofen amide (2HIBUA), thus relieving the growth inhibition of ibuprofen in Scenedesmus obliquus. This is the first complete pathway reported for the detoxification of ibuprofen transformation by a Gram-positive strain. These findings further our understanding of the microbial catabolism of the IBU.
研究领域:
环境微生物学
·
药物代谢
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生态毒理学
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产品关联
DOI
PubMed
高影响因子
3 Biotech
·
2020
·Vol. 10
·期 7
·1-10
·
IF 1.798
Peng Xiao-Lun,Zhao Wei-Jun,Wang Yuan-Shan,Dai Ke-Lei,Cen Yu-Ke,Liu Zhi-Qiang,Zheng Yu-Guo
Gibberellic acid (GA 3 ) is a natural plant growth hormone that has been widely used in agriculture and horticulture. To obtain higher GA 3 producing strains, the method of screening the strains for resistance to simvastatin was used after treatment with nitrosoguanidine (NTG) and gamma rays. The rationale for the strategy was that mutants showing simvastatin resistance were likely to be high GA 3 producers, as their activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is relatively more effective. GA 3 yield of mutant S109 increased by 14.2% than that of the original strain. The GA 3 production ability in mutant S109 remained relatively stable after ten generations. With the addition of 0.4 g glycerol on the 5th day during the fermentation process in Erlenmeyer flask, maximum GA 3 production of 2.7 g/L was achieved by this mutant, exhibiting 28.6% increase compared with original strain. Furthermore, we also achieved 2.8 g/L GA 3 and had a 33.3% increase with addition 20 g glycerol on the 5th day during the fermentation process in a 5-L bioreactor. Our results indicated efficient GA 3 production could be achieved on the condition that the supply of glycerol at the suitable conditions. This study would lay a foundation for industrial production of GA 3 .
研究领域:
植物学
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微生物学
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遗传学
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DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2020
·Vol. 11
·期 12
·6555-6563
·
IF 2.851
Hou Rongrong,Shi Jie,Ma Xiubing,Wei Huanran,Hu Jiajun,Tsang Yiu Fai,Gao Min-Tian
Abstract Phenolic compounds are widely used in different research fields, such as pesticides, medicines, and food. In this study, phenolic acids (PAs) were extracted from rice straw and were found to exhibit a strong inhibitory effect on Botrytis cinerea . B. cinerea mycelial growth and spore generation decreased by 86.18% and 69.10%, respectively, following 0.2 g/L phenolic acid treatment. Confocal microscopic images demonstrated that phenolic acids changed the morphology of B. cinerea . The addition of phenolic acids to B. cinerea- infected tomato leaves increased PAL (phenylalaninammo-nialyase) and PPO (polyphenol oxidase) activities, and decreased POD (peroxidases) and CAT (catalase) activities in the leaves, indicating that phenolic acids enhanced the tolerance of tomato leaves to B. cinerea by reducing oxidative stress. Chlorophyll fluorescence imaging revealed that phenolic acids could alleviate the destruction of the photosynthetic system of B. cinerea -infected leaves. These results provide new insight into the use of phenolic acids from rice straw, through which a complete green cycle of ecological production can be established. Graphic Abstract
研究领域:
植物病理学
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农业科学
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产品关联
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2021
·Vol. 4
·期 3
·552-561
·
IF 5.693
Yan Jia,Niu Yanfei,Wu Chunhua,Shi Zhengjun,Zhao Ping,Naik Nithesh,Mai Xianmin,Yuan Bingnan
As antimicrobial agents, essential oils (EOs) have been used by more and more companies as alternatives to traditional chemicals. In the study, seven EOs from Pinus massoniana , cinnamon , Star anise , Litsea cubeba , Cinnamomum camphora , Lemon eucalyptus , and Camphor from Guangxi were researched for their antifungal performances and chemical components. With the help of the diffusion in a disc way as well as the minimum inhibitory concentration (MIC) to three fungi, the antifungal impacts and the bamboo slices anti-mold tests of these EOs were studied. The compositions of these oils were identified. Litsea cubeba EO, Anise EO, and Cinnamon EO exhibited super antifungal activity against Trichoderma viride , Aspergillus niger , and Penicillium citrinum . Treated by Cinnamon EO, Anise EO, or Litsea cubeba EO, few molds were observed on the surface of bamboo chips, which showed that these EOs had a good antifungal effect on molds of bamboo. Graphical abstract Antifungal effect of seven essential oils on bamboo
研究领域:
植物学
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微生物学
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DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 5
·835
·
IF 5.1
Chuling Liu,Li Cheng,Mingtian Yang,Zhengli He,Yanan Jia,Li Xu,Yuansong Zhang
Monascusis a fungus widely used in food fermentation. This study employed microbial technology, combined with microscopic morphological observations and ITS sequence analysis, to isolate, purify, and identify 10 strains of red yeast mold from variousMonascusproducts. After the HPLC detection of metabolic products, the M8 strain containing the toxic substance citrinin was excluded. Using the EWM-TOPSIS model, the remaining nine safeMonascusstrains were evaluated for their inhibitory activities against pancreatic lipase, α-glucosidase, α-amylase, and the angiotensin-converting enzyme. The M2 strain with the highest comprehensive scores for lowering blood sugar, blood lipids, and blood pressure was selected. Its fermentation product at a concentration of 3 mg/mL had inhibition rates of 96.938%, 81.903%, and 72.215%, respectively. The contents of the blood lipid-lowering active substance Monacolin K and the blood sugar and blood pressure-lowering active substance GABA were 18.078 mg/g and 5.137 mg/g, respectively. This strain can be utilized for the biosynthesis of important active substances such as Monacolin K and GABA, as well as for the fermentation production of safe and effective functional foods to address health issues like high blood lipids, high blood sugar, and high blood pressure in people. This study also provides insights into the use of natural fungi to produce healthy foods for combating chronic diseases in humans.
研究领域:
微生物学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Hao Yang,Hanlin Ding,Xiaonuo Zhang,Xuegang Luo,Yu Zhang
In this study, a new surface-modified biobased composite was obtained by modifying the surface of fungal mycelium microspheres (FHMs) with polydopamine (PDA). A. niger mycelium/PDA microspheres (AM/PDA microspheres) were used as a highly efficient adsorbent for the removal of U(VI) ions. This study examined the effects of the initial pH, ionic strength, and electrolyte ions on the U(VI) adsorption capacity of the AM/PDA microspheres. The results confirmed that U(VI) was successfully adsorbed on the AM/PDA microspheres. We compared their adsorption capacity with that of other adsorbents, and the adsorption rate and adsorption capacity of the AM/PDA microspheres were found to be higher than those of the other adsorbents. Equilibrium was achieved after 300 min, and the AM/PDA microspheres had a maximum adsorption capacity of 250.70 mg/g. We aimed not only to investigate the factors that affect the adsorption process but also to provide a new mycelial-based composite adsorbent for U(VI)-contaminated water.
研究领域:
环境科学
·
生物材料学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 56
·期 12
·6591-6600
·
IF 3.713
Jiali Yang,Tengfei Wang,Jianbing Di,Yaping Liu,Xiaoling Hao,Yu Wang
Summary Although several studies have shown the ability of l -glutamate (glutamate) to mitigate the stress imposed by pathogens, the underlying mechanism is still in its infancy. To gain further knowledge, this study focussed on the effect of glutamate on primary nitrogen and carbon metabolisms during apple- Penicillium expansum interaction. The obtained result showed that glutamate could effectively restrict blue mould rot development in apples, but had no direct impact on fungal growth in vitro . The application of glutamate increased the level of nitrogen in apples, resulting in a disrupted balance of carbon and nitrogen. Consistently, the key enzymes glutamine synthetase (GS) and glutamate synthase (GOGAT) involved in the GS/GOGAT cycle, and the deaminating activity of glutamate dehydrogenase (GDH), forming 2-oxoglutarate and ammonium from glutamate, were promptly stimulated by glutamate. Interestingly, glutamate led to considerable consumption of the tricarboxylic acid (TCA) cycle intermediates, such as isocitric acid and citric acid, accompanied by the enhancement of malate dehydrogenase and succinate dehydrogenase activities. Collectively, exogenous application of glutamate might confer blue mould resistance in apples, at least in part, by redirecting host’s primary nitrogen and carbon metabolisms, such as the activation of the GS/GOGAT cycle, deaminating activity of GDH and the TCA cycle.
研究领域:
植物病理学
·
食品科学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Ke Hu,Rongyu Li,Feixu Mo,Yi Ding,Aiai Zhou,Xue Guo,Ruotong Li,Min Li,Minggui Ou,Ming Li
Dendrobium officinale Kimura et Migo is a traditional Chinese herbal medicinal plant . However, the frequent occurrence of soft rot disease (SRD) is one of the most harmful diseases in D. officinale production in recent years, which can seriously affect its yield and quality. In this study, the major pathogenic fungus (SR-1) was isolated from D. officinale with typical symptoms of SRD, and was identified as Fusarium oxysporum through morphological and molecular identification. The biological activities of five natural products were determined against F. oxysporum using a mycelial growth inhibition assay. The results showed that osthole had the highest antifungal activity against F. oxysporum , with an EC 50 value of 6.40 mg/L. Scanning electron microscopy (SEM) showed that osthole caused F. oxysporum mycelia to shrink and deform. Transmission electron microscopy (TEM) showed that the organelles were blurred and the cell wall was thickened in the presence of osthole. The sensitivity of F. oxysporum to calcofluor white (CFW) staining was significantly enhanced by osthole. Relative conductivity measurements and propidium iodide (PI) observation revealed that osthole had no significant effect on the cell membrane. Further experiments showed that the activity of chitinase and β-1,3-glucanase were decreased, and expression levels of chitinase and β-1,3-glucanase related genes were significantly down-regulated after treatment with osthole. In conclusion, osthole disrupted the cell wall integrity and dynamic balance of F. oxysporum , thereby inhibiting normal mycelial growth.
研究领域:
植物病理学
·
药用植物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF STORED PRODUCTS RESEARCH
·
2024
·
IF 2.7
Lingling Li,Chunhua Wu,Qian Chen,Zhengjun Shi,Kaimeng Xu,Yanfei Niu,Xiaoping Rao
The presence of chemical pesticides has an impact on human health and there is an urgent need for the development of green antifungal alternatives. This study investigated the freshness application of dehydroabietic acid-modified chitosan product (CSDA) mixed with three essential oil (litsea cubeba oil (LCO), wintergreen essential oil (WEO), cinnamon essential oil (CEO)). The antifungal activity of modified chitosan/essential oils was tested by the punching method and the antifungal mechanism was investigated by the transmission electron microscope (TEM). The results showed that fungal inhibition rate of CSDA-WEO on Penicillium digitatum reached 100% at a concentration of 0.05 μL/mL and on Penicillium italicum reached 100% at a concentration of 1 μL/mL. Both molds were not well inhibited by CSDA-LCO and CSDA-CEO. CSDA-WEO was able to affect the cell walls and energy metabolism of the molds, resulting in nutrient loss and death. Fruits were preserved with 0.1 mg/g CSDA and 1 μL/mL WEO. CSDA-WEO maintained the antioxidant activity of citrus , inhibited respiration and water loss, and extended shelf life up to 24 days. CSDA-WEO extended the shelf life of longan to 18 days and kiwi slices to 6 days. CSDA-WEO is expected to serve as an alternative to chemical fungicides and shows promise as a broad-spectrum fruit and vegetable preservative.
研究领域:
食品科学
·
微生物学
·
植物病理学
·
农业化学
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产品关联
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2016
·
IF 2.429
Jinwei Zhang,Zhangwei Han,Bo Teng,Wuyong Chen
For getting a clear and detail process of chromium tanned leather biodeteriorated with Penicillium sp. as a reference for future research and providing a cleaner chromium containing leather waste disposal method. The chromium tanned leather (CTL) and chromium tanned hide powder (CTHP) were biodeteriorated with the Penicillium . The CTL was biodeteriorated at first to verify the biodeterioration effect, and then the changes of the CTHP structure and thermal properties after the biodeterioration were analyzed by Scanning Electron Microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), Thermal Gravimetric Analyzer (TG) and Differential Scanning Calorimeter (DSC). The deterioration degree of the CTHP was characterized by the contents of hydroxyproline and total nitrogen in the filtrate, the Penicillium number was counted too during the process. The results showed that the CTL could be biodeteriorated with the Penicillium as shrinkage temperature and mass decreasing even though the biodeterioration degree was not ideal enough. After the process of CTHP biodeteriorated with the Penicillium , the surface morphology and structure of the CTHP were damaged. The thermal resistance and thermal denaturing temperature reduced which showed the detanning effect happened in the biodeterioration process. With the increasing of biodeterioration time, the hydroxyproline and total nitrogen in the filtrate increased. The Penicillium number maximized at 96 h and protease was produced in the process with enzymatic activity reaching to 9.23U mL −1 . These phenomena demonstrated the Penicillium grew through digesting chromium tanned leather and produced metabolites. To sum up, the biodeterioration process of chromium tanned leather with Penicillium was a synergistic effect of chromium detanning and the Penicillium growth.
研究领域:
微生物学
·
环境科学
·
皮革化学
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DOI
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-8
·
IF 4.38
Xu Xue,Naseri Ali,Houbraken Jos,Akbari Farzaneh,Wang Xiaodong,Zhao Rongfen,Zhang Hong,Najafzadeh Mohammad Javad,Deng Shuwen
Aspergillus species are emerging causative agents of non-dermatophyte mold onychomycosis. In this study, 48 Aspergillus isolates were obtained from patients with onychomycosis in Mashhad, Iran, during 2015–2018. The aim is to identify the Aspergillus isolates to the species level by using partial calmodulin and beta-tubulin gene sequencing and MALDI-TOF MS, and to evaluate their in vitro susceptibility to ten antifungal drugs: terbinafine, itraconazole, voriconazole, posaconazole, ravuconazole, isavuconazole, caspofungin, micafungin, anidulafungin and amphotericin B according to CLSI M38-A3. Our results indicate that A.flavus (n = 38, 79%) is the most common Aspergillus species causing onychomycosis in Mashhad, Iran. Other detected species were A. terreus (n = 3), A. tubingensis (n = 2), A. niger (n = 1), A. welwitschiae (n = 1), A. minisclerotigenes (n = 1), A. citrinoterreus (n = 1) and A. ochraceus (n = 1). Aspergillus flavus , A. terreus and A. niger isolates were correctly identified at the species level by MALDI-TOF MS, while all cryptic species were misidentified. In conclusion, A. flavus is the predominant Aspergillus species causing onychomycosis due to Aspergillus spp. in Mashhad, Iran. MALDI-TOF MS holds promise as a fast and accurate identification tool, particularly for common Aspergillus species. It is important that the current database of reference spectra, representing different Aspergillus species is expanded to increase the precision of the species-level identification. Terbinafine, posaconazole and echinocandins were i n vitro most active against the studies Aspergillus isolates and terbinafine could be the first choice for treatment of onychomycosis due to Aspergillus .
研究领域:
医学
·
真菌学
·
临床微生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）