Toxicology Research
·
2021
·Vol. 10
·期 4
·667-676
·
IF 3.524
Liu Rundong,Wang Yawei,Bai Lin,Wang Ruike,Wu Yingying,Liu Mengchen,Li Qiong,Ba Yue,Zhang Huizhen,Zhou Guoyu,Cheng Xuemin,Huang Hui
Sirtuin 1 (SIRT1), the NAD-dependent histone deacetylase, has been extensively investigated due to its cognitive protective effect. Studies suggest microRNAs (miRNAs) and histone modifications are key epigenetic regulators of gene expression and play important role in brain development. We previously showed that cognitive impairment by lead (Pb) was associated with downregulation of SIRT1, but the epigenetic role of this is unclear. Thus, we exposed 4-week-old male mice to 0.2% lead acetate solution for three months, and subsequently extracted brain homogenate from mice cortex and hippocampus at the age of 1, 4, and 16 months, respectively. In this study, we found that the protein level of SIRT1 was inhibited in the hippocampus and cortex of 16-month-old aged mice exposed to Pb. Moreover, changes in the levels of miR-138-5p and miR-141-3p, which were considered to the mechanistic target of SIRT1 by bioinformatic analysis, were negative correlations SIRT1 protein expression. We also found miR-34c-3p expression was increased in the cortex of mice at the age of 16 months. Collectively, our results showed the expression of neural SIRT1 and three selected microRNAs at different age nodes of mice for the first time of following Pb exposure. Our results suggest that additional efforts should focus on the consequences of early Pb exposure from an epigenetic perspective.
研究领域:
神经科学
·
分子生物学
·
毒理学
·
表观遗传学
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DOI
PubMed
高影响因子
Microfluidics and Nanofluidics
·
2016
·Vol. 20
·期 7
·1-11
·
IF 2.537
Bridle H.,Morton J.,Cameron P.,Desmulliez M. P. Y.,Kersaudy-Kerhoas M.
Public engagement activities based on microfluidics are being increasingly delivered and reported on in the literature. Here, we evaluate the success of a novel approach to microfluidics outreach recently undertaken with schoolchildren aged 12–13. Unlike previous work, a problem-based learning approach was adopted whereby participants were asked to design and test a microfluidic system to solve a research challenge. Our aim was to develop understanding of microfluidics design, manufacture and operation via involvement in the full engineering cycle of a product, from ideas to design, and from fabrication to test. This article demonstrates that problem-based learning is a successful method of public engagement with microfluidics, and we share our best practice, including activity design, supporting material produced for the project and an example case study detailing the types of chips produced by the participants. Furthermore, following an evaluation of the activity by all participants recommendations for delivery of this, or similar, activities are provided.
研究领域:
生物医学工程
·
科学教育
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DOI
高影响因子
Education and Information Technologies
·
2022
·Vol. 27
·期 6
·8203-8233
·
IF 3.666
Hu Yue,Su Chien-Yuan,Fu Anna
In recent years, increased attention has been given to programming instruction for primary and secondary students. Several game-based programming learning platforms, such as Code.org, Lightbot, and Run Marco, have been created to offer enticing, enjoyable, and visualizable programming learning conditions that facilitate student interest and engagement in learning programming. However, few studies have explored the perceptions of younger adolescents regarding the use of game-based programming learning. The study aims to adopt a technology acceptance model rooted in perceived enjoyment, computer self-efficacy, perceived visual attractiveness, system response, and challenge to investigate the intentions of younger adolescents regarding the use of game-based programming learning supported by Code.org and to analyze the moderating influence of gender and grade level. Data obtained from 1,539 primary (grades 3–6) and secondary (grades 7–8) students in eastern China through questionnaire surveys were analyzed using structural equation modeling. The results showed that perceived usefulness, perceived enjoyment, and computer self-efficacy all had a direct impact on students’ intentions to use game-based programming learning, while perceived visual attractiveness, system response, challenge, and perceived ease of use all had an indirect effect. Moreover, we also found that gender and grade level had moderating effects on student game-based programming learning. Overall, this study offered insight into younger adolescents’ intentions toward game-based programming learning.
研究领域:
教育心理学
·
计算机教育
·
青少年认知发展
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DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 23
·16228-16239
·
IF 3.9
Peipei Huang,Qiongfang Nie,Yu Tang,Shunshun Chen,Kaiyuan He,Mingguo Cao,Zefeng Wang
Dental erosion is a common problem in dentistry, and it refers to the chronic pathological loss of dental hard tissues due to nonbacterially produced acids, primarily caused by the exposure of teeth to exogenous acids. Dietary factors play a pivotal part in the pathogenesis of dental erosion, with a high intake of acidic beverages leading to an increased prevalence of dental erosion in adolescents. Fluoride is mainly used in clinical practice to prevent dental erosion. However, long-term fluoride intake may lead to chronic fluorosis symptoms caused by fluoride overdose. Nano-coatings on dental surfaces have become a popular area of research in dental materials in recent years. The objective of this study was to develop a novel nerol-segmented waterborne polyurethane nano-coating to protect teeth from direct contact with an acidic environment and to provide a safe, effective method for preventing dental acid erosion.
研究领域:
牙科
·
材料科学
·
纳米技术
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DOI
PubMed
高影响因子
BMC Ophthalmology
·
2023
·Vol. 23
·期 1
·1-24
·
IF 2
Zhang Zengrui,Mu Jingyu,Wei Jing,Geng Haoming,Liu Chunmeng,Yi Wenhua,Sun Yue,Duan Junguo
Background Refractive errors are one of the most common ocular conditions among children and adolescents, with myopia showing an increasing prevalence and early onset in this population. Recent studies have identified a correlation between refractive errors and ocular biometric parameters. Methods A systematic search was conducted in electronic databases including PubMed, EMBASE, Cochrane Library, Web of Science, and Medline from January 1, 2012, to May 1, 2023. Various ocular biometric parameters were summarized under different refractive states, including axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD), lens thickness (LT), corneal curvature (CC), Corneal curvature radius (CR),axial length-to-corneal radius ratio (AL/CR ratio), choroidal thickness (ChT), retinal thickness (RT), retinal nerve fiber layer thickness (RNFL), and retinal blood density (VD). The differences in these parameters among different refractive states were analyzed using Stata software with fixed or random-effects models, taking into account the assessed heterogeneity level. Results This meta-analysis included a total of 69 studies involving 128,178 eyes, including 48,795 emmetropic eyes, 60,691 myopic eyes, 13,983 hyperopic eyes, 2,040 low myopic eyes, 1,201 moderate myopic eyes, and 1,468 high myopic eyes. The results of our study demonstrated that, compared to the control group (emmetropic group), the myopic group and low, moderate, and high myopic groups showed significant increases in AL, AL/CR ratio, and ACD, while the hyperopic group exhibited significant decreases. Compared to the control group, the myopic group had a significantly increase for CC, while CR, CCT, perifoveal RT, subfoveal ChT, foveal ChT, parafoveal ChT, perifoveal (except nasal) ChT, and pRNFL (except temporal) significantly decreased. Compared to the control group, the hyperopic group had a significantly increase for subfoveal ChT, foveal ChT, parafoveal ChT, perifoveal ChT, and nasal pRNFL. Compared to the control group, the low and moderate myopic groups had a significantly decreases for the CCT, parafoveal RT (except nasal), perifoveal RT (except nasal), and pRNFL (except superior and temporal). Compared to the control group, the high myopic group had a significantly increase for CR, while LT, perifoveal ChT (except nasal), parafoveal RT, perifoveal RT, and pRNFL (except temporal) had significant decreased. Conclusion The changes of ocular biometric parameters in children and adolescents are closely related to refractive errors. Ocular biometric parameters devices, as effective non-invasive techniques, provide objective biological markers for monitoring refractive errors such as myopia.
研究领域:
眼科
·
视觉科学
·
流行病学
·
生物医学工程
·
儿科
✓ 已验证
DOI
高影响因子
CHILDRENS LITERATURE IN EDUCATION
·
2019
·Vol. 51
·期 3
·411-429
·
IF 0
Guanio-Uluru Lykke
This article analyses the role of plants in three well publicised Nordic climate fictions for young adults: Memories of Water by Emmi Itäranta, originally published in 2012, The World According to Anna (2013) by Jostein Gaarder, and Bouvetøya 2052 (2015) by Lars Mæhle. Departing from the wider scholarly field of ecocritical theory, the study draws on the developing field of cultural plant studies to examine the role allocated to plants in these fictional depictions of climate change. The analysis is based on a quantitative counting and sorting of all references to plants in the analysed fictions. The aim of this exercise is to contribute to theoretical reflections on climate fiction (cli-fi) as a literary form and to say something about the kind of literary thinking with, and about, plants that currently informs (Nordic) climate fiction for young adults, given that plants are highly important to the global climate. While broadening the discussion of climate change fiction to include a consideration of plants, the article further contributes to theoretical reflection on cli-fi through its Nordic perspective. In dialogue with Adam Trexler’s work on Anglo-American climate change fiction, the Nordic fictions examined here display both similar and diverging patterns of engagement with climate change, something that highlights the importance of reflecting on the genre with reference to a wide spectrum of local literatures.
研究领域:
生态文学
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环境人文学
·
当代文学研究
·
青年文化研究
·
气候变化与社会
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DOI
BRAIN RESEARCH BULLETIN
·
2022
·
IF 3.715
Lin Bai,Yingying Wu,Ruike Wang,Rundong Liu,Mengchen Liu,Qiong Li,Yue Ba,Huizhen Zhang,Guoyu Zhou,Fangfang Yu,Hui Huang
As a ubiquitous toxic heavy metal, lead (Pb) exposure is known to be implicated in the onset and development of neurodegenerative diseases which may cause more serious health hazards with age and the accumulation of Pb in the body. Autophagy is the main degradation route for abnormal aggregated proteins and damaged cell organelles. Here, we aimed to study the effects of adolescent Pb exposure on autophagy at different life nodes. In this study, we developed a time-series model of Pb exposure in mice and randomly divided 4-week-old male C57BL/6 mice into six groups (4 C, 13 C, 16 C, 4Pb, 13Pb and 16Pb). Mice in Pb groups was consumed deionized water containing 0.2 % Pb(Ac) 2 for 3 months and then reared to anticipated life nodes, while the control group consumed deionized water. Western blot and Real-time qPCR were used to assess the effects of developmental Pb exposure on individual components of the autophagy machinery and modulation of microtubule-associated protein 1 light chain 3 (LC3) at each age stage. Our results showed that Pb exposure during adolescence reduced the p-mTOR/mTOR ratios with enhanced expression of Beclin-1, Atg12 and Atg7in both the hippocampus (HPC) and prefrontal cortex (PFC) of senescent mice while upregulation of LC3II/LC3I ratios and p62 suggested that autophagy mediates degradation was interrupted. Overall, we confirm that Pb exposure during adolescence promotes autophagic processes in the aged mice brain and that autophagic degradation is hindered, ultimately leading to a failure of autophagic degradation.
研究领域:
毒理学
·
神经科学
·
分子生物学
·
发育生物学
·
衰老研究
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Lin Lin,Siyuan Li,Que Liu,Xingxing Zhang,Ying Xiong,Shaoyun Zhao,Liyue Cao,Jiaxuan Gong,Yaping Liu,Rong Wu
Objective: This study aimed to investigate the potential relation between the retarded growth of skeletal muscle (SM) and dysbiosis of gut microbiota (GM) in children with asthma, and to explore the potential action mechanisms of traditional pediatric massage (TPM) from the perspective of regulating GM and short-chain fatty acids (SCFAs) production by using an adolescent rat model of asthma.Methods: Male Sprague-Dawley rats aged 3weeks were divided randomly into the 5 groups (n=6~7) of control, ovalbumin (OVA), OVA + TPM, OVA + methylprednisolone sodium succinate (MP) and OVA + SCFAs. Pulmonary function (PF) was detected by whole body plethysmograph, including enhanced pause and minute ventilation. Airway allergic inflammation (AAI) status was assessed by concentrations of OVA-specific immunoglobulin E in plasma, interleukin (IL)-4 and IL-1β in bronchoalveolar lavage fluid via ELISA assay. SM mass was assessed by using cross-sectional areas of diaphragm muscle and gastrocnemius via hematoxylin and eosin staining. GM and SCFAs production were detected by 16S rDNA sequencing and GC-MS, respectively. The protein and gene expressions of free fatty acid receptor 2 in SM were detected by using immunohistochemical staining and qRT-PCR, respectively. qRT-PCR was used to detect other relative gene expressions that were closely related with SM mass. The activity of insulin-like growth factor-1 (IGF-1)/protein kinase B (PKB/AKT) pathway in SM was detected by western blotting test.Results: OVA exposure caused obvious AAI and poor PF in adolescent rats. OVA-exposed adolescent rats had a retarded growth of SM mass and inhibited activity of IGF-1/AKT pathway, which was related with GM dysbiosis, reduced SCFAs production and FFAR2 expressions in SM. TPM efficiently enhanced the SM mass, along with alleviating AAI and improving PF. TPM activated IGF-1/AKT pathway in SM, which was closely related with correcting GM dysbiosis, enhanced SCFAs production and FFAR2 expressions.Conclusion: The retarded growth of SM mass and inhibition of IGF-1/AKT pathway existed in OVA-exposed adolescent rats, which was related with GM dysbiosis, reduced SCFAs production and FFAR2 expressions in SM. TPM efficiently enhanced the SM mass, at least, partially via regulating GM, enhancing SCFAs production and activating FFAR2-IGF-1/AKT pathway.
研究领域:
儿科学
·
免疫学
·
药理学
·
微生物学
·
肌肉骨骼系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）