Foods
·
2023
·Vol. 12
·期 3
·463
·
IF 5.2
Yunxia Cheng,Cui Wu,Zhenying Liu,Pingping Song,Bo Xu,Zhimao Chao
Qingpi, the dried immature pericarp ofCitrus reticulataBlanco, is a commonly used medicinal food with some health-promoting benefits. In general, it is essential that Qingpi be stored for a period of time, but there are no reports about the number of storage years needed to obtain the best quality of Qingpi. Our aim was to determine the best storage time of Qingpi by studying the physicochemical properties and metabolite changes in product stored from 1 to 5 years. As a result, the color of Qingpi became darker during storage. Both the levels of three flavonoids (hesperidin, nobiletin, and tangeretin) and total flavonoids (TFs) and the antioxidant activity decreased during storage and the total phenolics (TPs) content fluctuated during storage. Cluster analysis was performed on the color parameters measured using a color difference meter, revealing that the color of Qingpi differed before and after 3 years of storage. A total of 9 special differential metabolites were identified that could be used to distinguish the storage years of Qingpi. This is the first study to report the quality changes of Qingpi during storage. The optimized results of the quality evaluation indicated that Qingpi should be stored for no more than 3 years.Keywords:Qingpi;storage years;quality changes;physicochemical characteristics;color;flavonoids;antioxidant activity;metabolites
研究领域:
药理学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2021
·Vol. 32
·期 6
·1152-1161
·
IF 3.373
Xiaofang He,Sisi Huang,Mengmei Wu,Menghua Wu,Ying Zhang,Zhiguo Ma,Li Liu,Hui Cao
Introduction Citri Reticulatae Pericarpium Viride (Qing Pi in Chinese) is a clinically effective Chinese herb, which contains biologically valuable flavonoids. Qing Pi is divided into two commodity specifications, Si Hua Qing Pi (SHQP) and Ge Qing Pi (GQP), based on the harvesting time. The flavonoid contents in Qing Pi from different origins and commodity specifications may vary significantly, which will affect their therapeutic functions. Thus, it is crucial to set up a reliable and comprehensive quality evaluation method for flavonoid analysis in Qing Pi. Objectives We aimed to establish a rapid and sensitive ultrahigh-performance liquid chromatography method coupled with diode-array detection and high-resolution mass spectrometry (UPLC-DAD-HRMS) for identification and quantification of ten flavonoids in Qing Pi. Chemometric methods were further applied to distinguish Qing Pi of different origins and specifications. Methodology An UPLC-DAD-HRMS method was developed for the simultaneous separation and quantification of ten flavonoids in 46 batches of Qing Pi samples from different sources in China. Chemometric approaches were applied to discriminate Qing Pi from different origins and commodity specifications. Results The chemometric procedures (i.e., hierarchical clustering analysis and principal component analysis) were employed to identify the differences of Qing Pi samples with different origins and commodity specifications. The results showed that the contents of ten flavonoids in Qing Pi samples of different origins were significantly different, and the same results were found out between SHQP and GQP. Conclusions This study provides a chemical basis for quality control of Qing Pi.
研究领域:
中药学
·
分析化学
·
植物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Jingbin Chen,Yali Liu,Yonggui Song,Huihui Liang,Genhua Zhu,Bike Zhang,Liangliang Liao,Jian Luo,Ming Yang,Dan Su
Ethnopharmacological relevance Citri Reticulata Pericarpium Viride (also known Qing-Pi or QP) is a plant in the Rutaceae family, QP is a traditional Qi-regulating medicine in Chinese medicine that is compatible with other Chinese medicine components and has extensive clinical practice in treating anxiety and depression. Reports on the pharmacological effects of QP have demonstrated its neuroprotective effects and antioxidant capacities. Numerous pharmacological benefits of QP are attributed to its antioxidant abilities. Anxiety disorders are a broadly defined category of mental illnesses. Oxidative stress and an imbalance in the antioxidant defense system are typical pathological features of these disorders. Aim of the study The aim of this study was to evaluate the effects of QP essential oil on anxiety using animal models and investigate the underlying neurobiological mechanisms. Materials and methods This study aimed to develop an animal model of anxiety using chronic restraint stress and investigate the effects of inhalation of Citri Reticulata Pericarpium Viride essential oil on anxiety-like behavior, olfactory function, and olfactory bulb neurogenesis in mice with anxiety. Results The results showed that long-term chronic restraint stimulation caused a decrease in olfactory function, significant anxiety-like behavior, and a notable reduction in the number of neurons in the olfactory bulb. However, inhalation of Citri Reticulata Pericarpium Viride essential oil reversed these effects, improving the olfactory function, neuro-stimulating effect, alleviating anxiety-like behavior, and regulating theta (4-12Hz) oscillation in the hippocampus DG area. These effects were associated with changes in the expression levels of glutamate receptor NMDAR and NeuN in olfactory bulb. Conclusions The study revealed that mice with anxiety induced by chronic restraint stress exhibited significant olfactory dysfunction, providing strong evidence for the causal relationship between anxiety disorders and olfactory dysfunction. Moreover, QP essential oil has the potential to be developed as a therapeutic drug for anxiety disorders, in addition to its role as a complementary anxiolytic.
研究领域:
药理学
·
神经科学
·
民族药理学
·
行为科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Longwei Jiang,Fenghui Wang,Xinyan Xie,Cancan Xie,Anqi Li,Ning Xia,Xue Gong,Huajiang Zhang
The aim of this work was to develop active packaging film by using chitosan/guar gum (CG) film matrix and walnut green husk extract (WE), for preservation of fresh-cut apple. WE was used as cross-linking agent to improve physicochemical properties, and as active substances to enhance antioxidant activity of CG films. Fourier transform infrared spectroscopy and scanning electron microscopy results showed WE formed intermolecular hydrogen bond interactions with the film matrix, and microstructures of the film were more compact. With the increase of WE content (0–4 wt%), the mechanical properties of composite films were significantly enhanced, while permeability of water vapor and oxygen was significantly decreased ( p < 0.05). When the amount of extract reached 4 wt%, the DPPH radical scavenging activity of composite film was significantly increased to 94.59%. CG-WE and CG films were used as active packaging materials to preserve fresh-cut apple. When stored at 4 °C for 10 days, CG-WE films showed better performance in reducing firmness, weight loss, total soluble solids and inhibiting browning and microbial growth of fresh-cut apples. Therefore, as a new type of active food packaging material, CG-WE films have good physical properties, and great potential in ensuring food quality and extending shelf life.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2018
·
IF 1.845
Binbin Shi,Wene Zhang,Xue Li,Xuejun Pan
The phenolics content and antioxidant activity of walnut (Juglans sigillata Dode) green husks were analysed during different stages of development. Contents of total phenolics and total flavonoids decreased from April 25th to June 7th, then increased during June and July, and decreased again at the beginning of August. Thirteen types of phenolic compounds were identified using HPLC of which juglone had the highest content. Because of seasonal variations, the trends of different phenolic compounds were different. Contents of both total phenolics and total flavonoids were strongly correlated with antioxidant activity; however, compared with totals, less significant correlations were observed between individual phenolic compounds and antioxidant activities.
研究领域:
植物科学
·
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
CURRENT PHARMACEUTICAL DESIGN
·
2023
·
IF 3.1
Chen Chao,An Na,Pang Defeng,Cheng Yuanyuan,Chen Yingjie,Feng Xuefei,Lei Haoqi,He Wenqian,Yang Baofeng,Zhang Yan,Zhao Xin
Background: Colorectal cancer (CRC) is the most common type of gastrointestinal tumor, but the
available pharmacological treatment is insufficient. As a traditional Chinese medicine, the green walnut husks
(QLY) exhibit anti-inflammatory, analgesic, anti-bacterial and anti-tumor effects. However, the effects and
molecular mechanisms of QLY extracts on CRC were not yet made known.
Objective: This study aims to provide efficient and low toxicity drugs for the treatment of CRC. The purpose of
this study is to explore the anti-CRC effect and mechanism of QLY, providing preliminary data support for clinical
research of QLY.
Methods: Western blotting, Flow cytometry, immunofluorescence, Transwell, MTT, Cell proliferation assay,
and xenograft model were used to perform the research.
Results: In this study, the potential of QLY to inhibit the proliferation, migration invasion and induce apoptosis
of the mouse colorectal cancer cell line CT26 in vitro was identified. The xenograft tumor model of CRC noted
that QLY suppressed tumor growth without sacrificing body weight in mice. In addition, QLY-induced apoptosis
in tumor cells through NLRC3/PI3K/AKT signaling pathway was revealed.
Conclusion: QLY regulates the levels of mTOR, Bcl-2 and Bax by affecting the NLRC3/PI3K/AKT pathway
to promote apoptosis of tumor cells, suppressing cell proliferation, invasion and migration, and subsequently
preventing the progression of colon cancer.
研究领域:
肿瘤学
·
药理学
·
中医药
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Zhao Xiaolan,Ying Jiabao,Wang Zhuochen,Wang Yulu,Li Zhen,Gu Tianyi,Liu Shujun,Li Yulong,Liu Bing,Xin Fengjiao,Wen Boting
Introduction: Walnut green husk (WGH) is a waste byproduct from walnut industry. However, it is not well-known about its bioactive effect on human gut health.Methods: This study conducted in vitro digestion and fermentation experiments to study the bioactive effect of WGH.Results: Microbial fermentation was the primary mechanism to efficiently release phenolics and flavonoids, resulting in more excellent antioxidant capacities (DPPH, ABTS, and FRAP assays), which reached a highest value with 14.82 ± 0.01 mg VcE/g DW, 3.47 ± 0.01 mmol TE/g DW, and 0.96 ± 0.07 mmol FeSO4·7H2O/g DW, respectively. The surface microstructure of WGH became loose and fragmented after microbial fermentation. The analytical results of gut microbiota demonstrated that WGH could significantly increase the relative abundance of Proteobacteria in phylum level and Phascolarctobacterium in genus level while certain pro-inflammatory bacteria (such as Clostridium_sensu_stricto_1, Dorea, Alistipes, and Bilophila) was inhibited. Additionally, 1,373 differential metabolites were identified and enriched in 283 KEGG pathways. Of which some metabolites were significantly upregulated including ferulic acid, chlorogenic acid, umbelliferone, scopolin, muricholic acid, and so forth.Discussion: These results indicated that WGH could have antioxidant and anti-inflammatory activities in the human gut, which could improve the economical value of WGH in the food industry.
研究领域:
食品科学
·
营养学
·
微生物学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2022
·
IF 2.65
Qixuan Mu,Yaping Zhang,Ying Cui,Xin Chai,Junlian Liu,Yongzhi Li,Huijuan Yu,Yuefei Wang
As the representatives of closely related Chinese medicinal materials (CMMs) originated from Rutaceae family, Aurantii fructus immaturus (AFI), Aurantii fructus (AF), Citri reticulatae pericarpium viride (CRPV), and Citri reticulatae pericarpium (CRP) have better functions in regulating qi and promoting gastrointestinal motility. However, differences in the quality of closely related Citrus CMMs have not yet been revealed until now. Herein, this study focused on the systematic differentiation and in-depth understanding of closely related Citrus CMMs by a strategy integrating chemometrics and network pharmacology. Determined by ultra performance liquid chromatography, the content of nine flavonoids showed obvious fluctuations in the decoction pieces from different species (Citrus aurantium Linnaeus and Citrus reticulate Blanco) with decreasing levels in the samples of ripe fruits. Decoction pieces from the different species and ripening stages were well distinguished by orthogonal projection to latent structure-discriminate analysis (OPLS-DA) and cluster analysis. As a result, four active components including narirutin, naringenin, hesperidin, and 3,5,6,7,8,3′,4′-heptemthoxyflavone were filtered out by variable importance for the projection (VIP) value (VIP > 1.0), which were regarded as chemotaxonomic markers. Furthermore, a components-targets-diseases network was constructed via ingenuity pathway analysis (IPA), and the correlations were predicted between four chemotaxonomic markers, 223 targets, and three diseases including colitis, breast cancer, and colorectal cancer. The obtained results will be of great significance for identifying closely related Citrus CMMs and conduce to improving the resource utilization of CMMs.
研究领域:
药理学
·
分析化学
·
网络生物学
·
中医药
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）