MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Shan Huang,Pingping Mu,Guixin Li,Yutong Zhao,Silin Li,Yi Fang,Fuxiang Wei,Qi Xiao
Cu, B, N, S co-doped CDs with Cu–N₂O₂ sites were hydrothermally synthesized. • Cu-CDs function as both a red-emitting fluorophore and a peroxidase nanozyme in a single nanostructure. • The ·O 2 − generated by the nanozyme oxidizes TMB, quenching fluorescence and producing color. • AMP strongly adsorbs to the Cu site, scavenges ·O 2 − , and restores the fluorescence. • The dual-mode platform detects AMP at LODs of 0.78 μM (fluorescence) and 0.62 μM (colorimetry). Integrating optical signaling and enzymatic catalysis into a single nanostructure offers a promising route for biosensing. This work presents bifunctional copper-doped carbon dots (Cu-CDs) that serve simultaneously as red-emitting fluorescent probes (emission at 602 nm, quantum yield 15.84%) and as peroxidase-mimicking nanozymes for dual-mode detection of ampicillin (AMP). Hydrothermal treatment of 2,5-diaminotoluene sulfate and 3-aminophenylboronic acid with Cu 2+ produces spherical Cu-CDs (2.68 ± 0.15 nm) featuring well-defined Cu–N 2 O 2 coordination sites. Electron paramagnetic resonance spectroscopy and radical scavenging experiments confirm that the Cu–N 2 O 2 sites catalyze H 2 O 2 to generate of superoxide radicals (·O 2 − ), which oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to coloured oxTMB. The oxTMB absorbs strongly at 674 nm, spectrally overlapping with the Cu-CDs emission and quenching it through an inner filter effect-dominated static quenching mechanism. Density functional theory calculations reveal a catalytic cycle mediated by a Cu 2+ /Cu + redox couple and further demonstrate that AMP adsorbs much more strongly (−2.425 eV) than TMB (−1.578 eV) on the Cu–N 2 O 2 site. Consequently, AMP suppresses oxTMB formation not by homogeneous radical scavenging but through surface-adsorption-driven competitive inhibition, leading to fluorescence recovery and a decrease in absorbance at 674 nm. This dual-readout platform quantifies AMP over 1–150 μM (fluorometric, LOD 0.78 μM) and 1–80 μM (colorimetric, LOD 0.62 μM). It exhibits excellent selectivity against common ions, amino acids and various antibiotics, including structurally similar β-lactams (penicillin G, penicillin V, amoxicillin, cephalexin). Validation with real water samples and pharmaceutical preparations (ampicillin capsules and injections) gives recoveries of 90.40–109.20%. The integrated probe provides a robust self-validating strategy for environmental monitoring and pharmaceutical quality control. Download: Download high-res image (172KB) Download: Download full-size image
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Yi Jintao,Fu Nani,Zhong Wenhui,Liu Xia,Zhou Anqi,Li Xun
Amoxicillin (AMO), a penicillin antibiotic, and ciprofloxacin (CIP), a quinolone antibiotic, are widely used to combat bacterial infections and are frequently co-administered to enhance therapeutic efficacy. However, improper use of AMO and CIP may lead to antibiotic residues in the environment, posing serious threats to human health. The visual detection of AMO and CIP remains significant challenges. Herein, the Eu-DPA@CQDs was used for the dual-mode detection of AMO and CIP, which was synthesized by CQDs doped in europium-DPA metal-organic framework. In the colorimetric mode, the catalase-mimicking Eu-DPA@CQDs oxidized TMB to blue oxTMB. Upon addition of AMO, the oxTMB was reduced, causing color fading. Subsequent introduction of CIP restored nanozyme activity, regenerating the blue signal and enabling dual-analyte antibiotic detection. In the fluorescence mode, the Eu-DPA@CQDs probe exhibited dual emission at 440 nm and 613 nm. Upon AMO addition, the fluorescence at both wavelengths was quenched, accompanied by a color change from red to purple. In the presence of CIP, the emission at 440 nm increased and redshifted to 490 nm, while the signal at 613 nm was quenched, resulting in a multicolor transition from red to cyan. The Eu-DPA@CQDs sensor enabled rapid and dual-mode detection of AMO and CIP. The colorimetric method exhibited wide linear ranges of 0.005–25 μM for AMO and 0.007–4.25 μM for CIP, with detection limits of 1.67 nM and 3.75 nM, respectively. A visible color transition from dark blue to colorless was observed with increasing AMO concentration, while the addition of CIP reversed the signal back to dark blue. Similarly, the fluorescence detection achieved wide linear ranges from 0.005 to 30 μM for AMO and 0.005 to 15 μM for CIP, with detection limits as low as 4.74 nM and 3.11 nM, respectively, also within only 1 min. As the concentration of AMO increased, the color shifted from red to purple. In contrast, increasing CIP concentration induced a color transition from red to cyan. These color transitions were both clearly visible to the naked eye. The sensor demonstrated excellent selectivity and anti-interference capability, providing a reliable platform for accurate, sensitive, and quantitative determination of both antibiotics in real samples. Graphical abstract
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高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Wang Nan,Su Xiaoyan,Nie Yuan,Dou Huashuo,Wang Jiaxi,Rui Minglang,Liu Yueying
Most studies focus on improving the
activity of nanozymes to build
a nanozyme-based “chemical tongue” for the sensitive
discrimination of multiple analytes. However, the construction of
sensing units with multidimensional signals is often overlooked, which
greatly limits the ultrasensitive identification of antibiotics and
even their subtypes. Herein, we have for the first time designed the
high performance of a “chemical tongue” by integrating
high peroxidase (POD)-like activity Prussian blue analogues (PBAs)
with multidimensional information from multiple oxidation products
of 3,3 ′ ,5,5 ′ -tetramethylbenzidine (TMB) as sensing units.
Initially, the bimetallic PBA with boosted POD-like activity is achieved
via combining FeRu metal doping with an acid-etching morphological
reconstruction strategy. More importantly, the catalytic activity
for this hollow-structure nanozyme with a specific activity of 73.3
U/mg is increased by approximately 10 times compared to the pristine
Prussian blue (PB) through the synergistic reconstruction of redox
couples and exposure of more active sites. And then, the one-electron,
one/two-electron, and two-electron oxidation products of TMB are achieved
by rationally controlling the concentration of the nanozyme, yielding
three chromogenic products as three sensing units with six-wavelength
channels. Therefore, the developed array is successfully applied to
the ultrasensitive detection of seven antibiotics with down to 0.1
μM and even the discrimination of three subtypes including aminoglycosides,
penicillins, and tetracyclines. The sensing mechanism depends on the
variable inhibition efficiency of multiple antibiotics on the nanozyme
catalytic activity. Furthermore, it enables identification of molar
ratios in binary mixtures and also exhibits quantitative analysis
of individual antibiotics over a broad concentration range from 0.1
to 200 μM. Moreover, with the assistance of optimizing various
machine learning, the concentration-independent recognition model
is built against this array, which enormously improves the discrimination
accuracy of seven antibiotics from 73.03% by Decision Tree (DT) to
96.63% by the Gaussian Process Classification (GPC) algorithm. The
outstanding discrimination capability of this array enables accurate
identification of seven antibiotics in three real food samples (honey,
milk, and chicken breast). This study provides a new perspective for
configuring high-feature sensor arrays in the application of food
and environmental samples.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Chenyan Lu,Dajie Zou,Ning Zhang,Huye Li,Yanyan Jia,Huan Wang
In this study, the hexane extract of Asters tataricus was used as the carbon source, and ethylenediamine and 4-bromophenylene diamine were selected as dopants to prepare nitrogen and bromine co-doped carbon dots (N,Br-CDs) by the hydrothermal synthesis method. The obtained fluorescent probe has a uniform particle size distribution, good water solubility and high stability, with a fluorescence quantum yield of 48.43%. Under 420 nm excitation conditions, N,Br-CDs exhibited the strongest fluorescence emission at 513 nm, indicating that the sensing system was in the “ON” state. Subsequently, TA was introduced as a quencher to induce static quenching of the N,Br-CDs system, significantly weakening the fluorescence intensity and switching the sensing system from “ON” to “OFF”. Finally, after EM was introduced into the N,Br-CDs/TA system, EM preferentially formed a complex with TA and weakened its quenching effect on the quantum dots, thereby achieving fluorescence recovery and returning the sensing system from the “OFF” state to the “ON” state. Based on the above mechanism, an “ON-OFF-ON” fluorescence sensing system with N,Br-CDs as the luminescent substrate was constructed for the sensitive detection of TA and EM. The results showed that the system exhibited good linear response to TA, with the linear ranges being 1.50–22.94 μM and 34.88–348.42 μM, respectively, and the limits of detection being 0.2168 μM and 2.1797 μM, respectively (LOD, S/N = 3). The linear range for EM was 1.93–377.08 μM, with a detection limit of 0.6693 μM. Furthermore, the sensor system was capable of quantitatively determining TA and EM in human serum, Huangshui River water, and milk matrices, demonstrating its potential for broad application in environmental monitoring and bioanalysis.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Jing Zhang,Kairu Liu,Xiaojuan Duan,Xuhao Wang,Wupeng Ge,Wenping Jin
Koji is used as a fermentation starter in Choujiu manufacture in northwestern China. Traditional natural Koji (NK) cultivates in open environments, which probably leads to diverse microflora, causes inconsistent quantities in Koji, then results in instable qualities of Choujiu. A native Choujiu producer has been purchasing NK from local workshop, while their Choujiu qualities fluctuate by the stability of NK. As a request from the producer, this study aimed for stable Koji and inheriting sensory traits of current products. Strains of mold, yeast, and LAB, separated from NK, were selected for Restored Koji (RK) making. Brewing performance of RK and NK were compared by analysis of the physicochemical properties and volatile flavor compounds (VFCs) in fermented mash, combined with sensory evaluation in corresponding Choujiu. Pure Koji from individual strains was separately cultured at 28 °C for 48 h, and then reconstituted for RK II at the proportion of mold Koji (M7 Koji): yeast Koji (Y3 Koji: Y4 Koji = 1:1): LAB Koji (L1 Koji) = 1:1:1, where M7 was Rhizopus oryzae , Y3 was Saccharomyces cerevisiae , Y4 was Saccharomycopsis fibuligera , and L1 was Pediococcus pentosaceus . Results showed RK II could meet the desired purpose of this study.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Yuanqian Xiong,Dong Zhang,Cuizhu Ye,Yu Wang,Xiangrui Deng,Danwen Deng,Chunhong Xiong,Jinsheng Zhang,Qianhui Gu,Ganhui Huang
Ciprofloxacin hydrochloride is widely used to treat animal diseases. However, its chronic abuse has caused severe accumulation in animal-derived foods , which has seriously damaged human health. Thus, in this study, a novel molecularly imprinted electrochemical sensor (MIES) was designed for selective and ultra-sensitive detection of ciprofloxacin hydrochloride (CPFX) in milk. The sensor was based on the molecularly imprinted polymers (MIPs) synthesized with poly-pyrrole (PPy) as the functional monomer , AuNPs and S-CoFe-MOFs (CoFe-Metal organic frameworks coated with chitosan) as the Supporting materials , and CPFX as the template molecule. The electrochemical behavior of MIPs/S-CoFe-MOFs/AuNPs/GCE was studied by several electrochemical methods, which showed a wider detection linear range (0.05 ∼ 500 ng·mL -1 ) and lower detection limit (0.0033 ng·mL -1 ) than those in previous reports. The optimization of experiments confirmed the good selectivity and stability of the sensors----- with the milk sample recovery of 89.04∼110.26%, and the relative standard deviations (RSD) of less than 5.5%, which was close to HPLC. In conclusion, the sensor we constructed has a high selectivity and stability potential in detecting CPFX in milk.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Daikun Li,Wei Zhan,Xinlei Gao,Qi Wang,LiPin Li,Jun Zhang,Guiyuan Cai,Wei Zuo,Yu Tian
Remediation of wastewater with solid waste-based adsorbents is of great environmental significance, but most current waste-based adsorbents suffer from low efficiency. In this work, aminated waste paper (WP-NH 2 ) membrane is successfully prepared by oxidation of various types of waste paper followed by grafting and crosslinking polyethyleneimine (PEI). WP-NH 2 shows extremely high adsorption for various anionic dyes and antibiotics. Specifically, the adsorption amount for methyl orange (MO) and tetracycline (Tc) are 2156.2 mg·g −1 and 253.7 mg·g −1 , respectively. WP-NH 2 is stable enough to be regenerated several times and still maintains high adsorption efficiency. Mechanistic studies show that the adsorption toward anionic MO is mainly through electrostatic interaction and hydrogen bonding, and the adsorption of antibiotic Tc relies on hydrogen bonding and Lewis acid-base reaction. Grafting PEI on the fiber surface enables efficient exposure of adsorption sites. Therefore, WP-NH 2 membrane can quickly, continuously and efficiently filter out MO and Tc from water. After 5800 L·m −2 MO and 3150 L·m −2 Tc filtration, the interception rate of the two contaminants is still higher than 90 %. Moreover, WP-NH 2 shows high selectivity and can be used for efficient separation of dye mixture. These results reveal that this advanced adsorbent made from waste paper has great potential application in industrial dye and antibiotic wastewater remediation.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Yalan He,Dongling Wu,Qian Liu,Jun Tang,Rong Chang,Tao Wang
Ag nanosheets (AgNS) exhibit enormous potential because of their unique structure and properties. However, the commonly used methods to synthesize AgNS have some disadvantages, such as long reaction time or high reaction temperature, or they involve toxicity and irritating corrosion solvents, which greatly limit their development and application. In this study, AgNS with edge lengths of 500–600 nm, and thicknesses of 25–30 nm are rapidly synthesized by ethylene glycol/deionized water (EG/DI water, solvent), poly(vinylpyrrolidone) (PVP, capping agent), ascorbic acid ( L -AA, reducing agent) at 0 °C in only 30 min. This study provides a fast and facile approach for the stable and large-scale production of AgNS. Benefiting from the unique structure of the prepared AgNS, as the SERS substrate it can be used to detect dye molecules (R6G), and achieves a superb enhancement factor of 8.7 × 10 7 . What’s more, it is also successfully realized practical detection of antibiotic benzylpenicillin sodium (NaBP) in milk. The results indicate that the AgNS produced by a simple and efficient method may serve as a high-efficiency SERS substrate for speedy chemical contamination detection.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 37
·17293-17302
·
IF 3.3
Tong Shao,Xiaoshuang Wang,Rentian Guan,Suyuan Zeng,Rui Li,Min Hong,Qiaoli Yue
In this study, carbonized polymer dots (CPDs) were first proposed for the quantitative detection of chlortetracycline (CTC) based on aggregation induced emission. CPDs were synthesized by a one-step hydrothermal assay using polyethylene imine as the sole precursor. CPDs showed bright blue fluorescence under UV light (λEx 350 nm and λEm 465 nm), with a quantum yield of 14.58%. There was a significant fluorescence enhancement for the CPDs + CTC system. When the concentration of CTC increased, the fluorescence color of CPDs displayed a significant change from bright blue to dark blue with the maximum emission shifting from 465 nm to 430 nm. Furthermore, the lifetime of CPDs + CTC increased from 7.12 ns to 11.26 ns, an increment of 4.14 ns, which was ascribed to the aggregation induced emission of CTC in the presence of CPDs. There was a good linear response between the fluorescence intensity of CPDs and the concentration of CTC from 0.1 μM to 120 μM with a limit of detection of 40 nM. Therefore, an excellent fluorescence probe was successfully prepared for the detection of CTC and its application in real samples was confirmed.
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Nanomaterials
·
2022
·Vol. 12
·期 18
·3241
·
IF 5.719
Hui Yang,Yunlong Wei,Xiufang Yan,Chao Nie,Zhenchun Sun,Likai Hao,Xiankun Su
Recycling waste biomass into valuable products (e.g., nanomaterials) is of considerable theoretical and practical significance to achieve future sustainable development. Here, we propose a one-pot hydrothermal synthesis route to convert waste tobacco stems into biomass-based N, S-codoped carbon dots (C−dots) with the assistance of carbon black. Unlike most of the previously reported luminescent C−dots, these biomass-based C−dots showed a satisfactory stability, as well as an excitation-independent fluorescence emission at ~520 nm. Furthermore, they demonstrated a pH-dependent fluorescence emission ability, offering a scaffold to design pH-responsive assays. Moreover, these as-synthesized biomass-based C−dots exhibited a fluorescence response ability toward tetracycline antibiotics (TCs, e.g., TC, CTC, and OTC) through the inner filter effect (IFE), thereby allowing for the establishment a smart analytical platform to sensitively and selectively monitor residual TCs in real environmental water samples. In this study, we explored the conversion of waste tobacco stems into sustainable biomass-based C−dots to develop simple, efficient, label-free, reliable, low-cost, and eco-friendly analytical platforms for environmental pollution traceability analysis, which might provide a novel insight to resolve the ecological and environmental issues derived from waste tobacco stems.Keywords:waste tobacco stems;biomass-based C−dots;tetracycline antibiotics;inner filter effect;environmental pollution traceability analysis
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JOURNAL OF CHEMICAL NEUROANATOMY
·
2022
·
IF 3.097
Liaoxi Tan,Yixin Hu,Xinyi Zhang,Chunlei Zhang,Chuchu Xi,Zhao Yang,Zhengyu Cao,Fang Zhao
Background and purpose Microglial activation plays an important role in the onset and progression of neuropathic pain by producing a variety of pro-inflammatory cytokines that interact with neurons to enhance neuronal hyperexcitability. Corydalis decumbens (Thunb.) pers., a traditional Chinese medicine has been used to treat mild cancer pain, dementia and to remit cerebral ischemia in clinics. Phenylphthalide isoquinolines are the major type of metabolites of C. decumbens and one of the derivatives, Corydecumine G (Cor G) has been shown to inhibit neuronal excitability . The present study aims to investigate the analgesic efficacy of Cor G in neuropathic pain rat model, the effects of Cor G on microglia activation and the possible mechanisms. Experimental approach Neuropathic pain was modeled using chronic constriction sciatic nerve injury (CCI) in rats. Western blot , immunofluorescence, and qRT-PCR were used to evaluate the levels of protein and mRNA. Key results Intraperitoneal administration of Cor G concentration-dependently ameliorates mechanical and thermo allodynia , suppresses CCI-induced p38/ERK phosphorylation and spinal cord microglia activation, and attenuates the expression levels of NO, inos , Tnf-α , Pge 2 in dorsal horn of L4-L6 spinal cord on the ligation side in CCI rats. Pretreatment with 30 μM Cor G decreased LPS-induced BV2 microglia activation, which occurred via the inos , Tnf-α , Il-1β , Il-6 and phospho-p38/ERK pathways. Conclusions and implications Taken together, we suggest that Cor G, the specific phthalide isoquinoline from traditional Chinese medicine Corydalis Decumbentis Rhizoma, may be promising for treatment of neuropathic pain.
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Advanced Science
·
2023
·
IF 15.1
Youqiang Fang,Xing Luo,Yanteng Xu,Zheng Liu,Rachel L. Mintz,Haiyang Yu,Xuan Yu,Kai Li,Enguo Ju,Haixia Wang,Zhaohui Tang,Yu Tao,Mingqiang Li
As a currently common strategy to treat cancer, surgical resection may cause tumor recurrence and metastasis due to residual postoperative tumors. Herein, an implantable sandwich-structured dual-drug depot is developed to trigger a self-intensified starvation therapy and hypoxia-induced chemotherapy sequentially. The two outer layers are 3D-printed using a calcium-crosslinked mixture ink containing soy protein isolate, polyvinyl alcohol, sodium alginate, and combretastatin A4 phosphate (CA4P). The inner layer is one patch of poly (lactic- co -glycolic acid)-based electrospun fibers loaded with tirapazamine (TPZ). The preferentially released CA4P destroys the preexisting blood vessels and prevents neovascularization, which obstructs the external energy supply to cancer cells but aggravates hypoxic condition. The subsequently released TPZ is bioreduced to cytotoxic benzotriazinyl under hypoxia, further damaging DNA, generating reactive oxygen species, disrupting mitochondria, and downregulating hypoxia-inducible factor 1 α , vascular endothelial growth factor, and matrix metalloproteinase 9. Together these processes induce apoptosis, block the intracellular energy supply, counteract the disadvantage of CA4P in favoring intratumor angiogenesis, and suppress tumor metastasis. The in vivo and in vitro results and the transcriptome analysis demonstrate that the postsurgical adjuvant treatment with the dual-drug-loaded sandwich-like implants efficiently inhibits tumor recurrence and metastasis, showing great potential for clinical translation.
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