Foods
·
2021
·Vol. 10
·期 11
·2735
·
IF 4.35
Chujun Liu,Yawen Guo,Bo Wang,Lan Chen,Kaizhou Xie,Chenggen Yang
A simple and sensitive gas chromatography–tandem mass spectrometry (GC–MS/MS) method was established for the quantitative screening of penicillin G residues in chicken and duck eggs (whole egg, yolk and albumen). The analyte was separated on a TG-1MS capillary column (30.0 m × 0.25 mm i.d., 0.25 μm) with an external calibration method and electron impact (EI) ionization. Samples were pretreated using an accelerated solvent extraction (ASE) procedure followed by solid-phase extraction (SPE) on HLB cartridges (60 mg/3 mL). The derivative, which was safer and easier to store than penicillin G, was obtained by reacting trimethylsilyl diazomethane (TMSD) with penicillin G. The method was validated by the following parameters: linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The matrix-matched calibration curves had good linearity (R2 ≥ 0.9994) within the concentration range of LOQ–200.0 µg/kg for penicillin G in the sample matrices. In the same concentration range, the accuracy, in terms of recovery, was 80.31–94.50%; the relative standard deviation (RSD), intra-day RSD and inter-day RSD ranged from 1.24 to 3.44%, 2.13 to 4.82% and 2.74 to 6.13%, respectively. The LODs and LOQs of penicillin G in the matrices were in the ranges of 1.70–3.20 and 6.10–8.50 μg/kg, respectively. The applicability of the GC–MS/MS method was demonstrated by the determination of poultry eggs obtained from local markets with no penicillin G residues.Keywords:penicillin G;GC–MS/MS;ASE;poultry eggs;SPE
研究领域:
分析化学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Lili Liu,Jun Wang,Jianan Li,Meng Zhang,Yunxiao Zhang,Rui Zhan,Jiaxin Li,Zhiping Wang
The efficient mineralization of antibiotics in production waste drainage (PWD) and fermentation residue leachate (FRL) is crucial for avoiding environmental accumulation and the generation of antibiotic-resistant bacteria and genes. In this study, the penicillin G (PG) containing PWD and FRL were treated by electrochemical enhanced heterogeneous activation of peroxymonosulfate with CoFe 2 O 4 nanoparticles (EC/CoFe 2 O 4 /PMS). The results indicated that the mineralization rate of PG did not improve significantly in EC/CoFe 2 O 4 /PMS system (39.0 %) with the same reaction condition ([CoFe 2 O 4 ] = 100 mg L -1 , [PMS] = 152 mg L -1 , [Na 2 SO 4 ] = 20 mM) of CoFe 2 O 4 /PMS system (28.1 %) coupled with 1 V cm −1 electric field. Meanwhile, the toxic intermediate product ( P 4) was detected. However, at the condition of catalyst saving mode (CSM, [CoFe 2 O 4 ] = 50 mg L -1 , [PMS] = 250 mg L -1 , [Na 2 SO 4 ] = 20 mM, E = 1 V cm −1 ), the mineralization rate of PG in artificial wastewater reached 69.1 %, which was further increased to 88.0 % and 76.5 % in the PWD and FRL, respectively. Although the removal rate of pollutants even exceeded 90 %, most of them were not meet the direct discharge requirements of China due to their high concentration in initial PWD and FRL. However, the ratio of BOD 5 /COD (represented the biodegradability) of PWD and FRL significantly increased from 0.18 and 0.37 to 0.47 and 0.79 at the end of the experiment, respectively, and the P 4 was not detected. Therefore, EC/CoFe 2 O 4 /PMS system with CSM operation mode could eliminate PG completely and achieve deep mineralization, which improved the biodegradability of production drainage, and might achieve complete mineralization of PG through combination of bioremediation methods.
研究领域:
环境科学
·
生物工程
·
废水处理
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Xu Yi,Wang Haiping,Bai Qingyan,Ma Shujuan,Bo Chunmiao,Ou Junjie
A carbon dot (CD) was prepared by o -phenylenediamine and water, which showed bright yellow fluorescence under ultraviolet light irradiation (λ = 580 nm), and verified good fluorescence quenching effect on penicillin G sodium (Png-Na). Using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinker, and Png-Na as a template, a kind of composite microsphere combining CD and molecularly imprinted polymer (MIP) was synthesized by surface-initiated atomic transfer radical polymerization (SI-ATRP). For reasons of comparison, we also prepared MIP without CD and non-imprinted polymers (NIPs). Through static and dynamic adsorption experiments, the maximum adsorption capacity was 47.05 mg g −1 and the equilibrium time was 30 min. High-performance liquid chromatography (HPLC) was utilized to determine the content of Png-Na in the spiked milk samples. A sensitive, rapid, and simple method for determination of Png-Na in food samples was developed. The utilized approach enabled the quantification of Png-Na within the concentration range 20–1000 μg L −1 (with a limit of detection of 5 μg L −1 ). The recoveries achieved were in the range 93.3–98.2%, with a relative standard deviation of 1.2–4.2%. The results demonstrated that CD@MIP possessed the capability of specific adsorption and fluorescence detection of Png-Na, enabling simultaneous detection and enrichment of Png-Na in real samples. Graphical abstract
研究领域:
食品科学
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分析化学
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材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Aiting Chen,Shuting Shi,Nana Tang,Yanping Wei,Chuanqin Zhou,Quanguo He,Jianhua Ding
Penicillin G (Pen G) residues enter the environment through pharmaceutical wastewater, agricultural runoff, medical waste, and animal manure, threatening ecosystems and human health. Therefore, developing a swift, efficient, and precise method for Pen G detection is crucial. Bismuth manganate (BiMn 2 O 5 ) was successfully synthesized using the chemical co-precipitation method. The synthesized BiMn 2 O 5 was then ultrasonically compounded with carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) in a chitosan (CS) solution. Subsequently, penicillinase (Pen X) was immobilized onto the CS/BiMn 2 O 5 /MWCNTs-COOH/GCE to augment the electrode’s catalytic function via enzymatic action, resulting in the Pen X/CS/BiMn 2 O 5 /MWCNTs-COOH/GCE composite electrode. Following optimization of the experimental conditions, quantitative detection of Pen G was conducted employing differential pulse voltammetry. The study revealed that the peak currents were wide linearly related to Pen G concentrations (0.0007–0.01, 0.03–1 µM), respectively. The detection limit was established at 0.14 nM, and the sensor demonstrated a high sensitivity with a value of 484.8 µA µM −1 . The recoveries from spiked milk and lake water samples were in the ranges of 94.4–106.3 % and 101.5–104.8 %, respectively. This work is crucial for protecting ecological balance, as it can monitor the misuse of Pen G, safeguard the health of aquatic ecosystems, and maintain human health.
研究领域:
分析化学
·
环境科学
·
生物医学工程
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产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 11
·4602-4607
·
IF 1.821
MingHui Li,WenShen He,LiJun Zhang,ChengLi Duan
A capillary zone electrophoresis method was established for simultaneous analysis of penicillin G and penicillin acid in milk. Milk samples were deproteinized and the analytes were extracted with acetonitrile. Separation of target compounds was performed through electrophoresis. Under optimum conditions, the proposed method demonstrated good correlation coefficients (R > 0.9953), precision (<6.2%), accuracy (89.2–96.8%), and limits of detection and quantification of penicillin G and penicillin acid (LOD, 0.01 and 0.5 μg mL−1, LOQ, 0.04 to 1.7 μg mL−1, respectively). The proposed method for the determination of penicillin and penicillin acid in milk is rapid, convenient and low-cost, and can measure β-lactamase activity in milk. The thermal stabilities of penicillin G, penicillin acid, and β-lactamases in milk were satisfactory when the temperature was lower than 303 K. When the temperature was higher than 353 K for 5 h, β-lactamase tended to be inactivated completely. When the temperature was 373 K, penicillin G and penicillin acid were easily degraded.
研究领域:
分析化学
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食品科学
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微生物学
✓ 已验证
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2020
·Vol. 14
·期 4
·2087-2098
·
IF 1.648
Ntakatsane Moeketsi,Chen Ping,Liu Jingsheng,Xu Linlin,Dong Na,Mosebi Poloko,Adoko George,Matebesi Puleng
Antibiotic residues in animal-derived food products such as milk pose a potential health risk to consumers. A portable antimicrobial screening test plate was fabricated particularly for on-farm testing of milk. A cold-water soluble compound gelling agent composed of guar gum (GG) and konjac glucomannan (KG) was prepared and its properties were optimized for enhancing performance of the test plate. A ratio of 4:6 (GG:KG) showed improved gel strength, cohesiveness, transmittance, and water diffusion of the compound gel. Microbial colony number and size were also increased. The test plate displayed a minimum inhibition concentration of 4 µg/L penicillin G and clearer zone of inhibition. Detection time was also significantly reduced to approximately 9–15 h compared to the conventional agar plate method. The plastic film test plate with optimized compound gel demonstrated tremendous potential for detection of antibiotics in milk.
研究领域:
食品安全
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2011
·
IF 2.692
Pixiang Wang,Xiangyu Gong,Erzheng Su,Jingli Xie,Dongzhi Wei
A facile pretreatment method was developed to obtain high specific activity of PGA from recombinant E. coli cells for preparing immobilized enzyme with high activity, which coupled selective extraction of PGA with butyl acetate and adsorption of butyl acetate with active carbon. Butyl acetate (5%, v/v) led to a 92.0% release of PGA, the specific activity of which in the extracting solution was twice that obtained by sonication. The negative effect on PGA immobilization due to residual butyl acetate in the extracting solution was removed by adding active carbon (8%, w/v). The final PGA solution meets requirement for industrial immobilization while eliminating the high cost of a traditional purification process. This pretreatment method developed in this work is simple, highly performing and cost-effective. It has been employed in a 10 tons/year immobilized PGA production line.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Jiali Zhang,Zhaokun Xiong,Jian Wei,Yonghui Song,Yuezhong Ren,Dongyao Xu,Bo Lai
A cerium-loaded natural zeolite (CZ) was synthesized for catalytic ozonation of penicillin G (PG) in this work. The effects of key factors including Ce loading mass, the initial solution pH, catalyst dosage and ozone dosage were elucidated. Under the optimal conditions: Ce loading mass = 4%, CZ dosage = 2 g/L, ozone dosage = 6 mg/min and the initial solution pH = 4.5, the removal efficiency of PG (50 mg/L in 1 L reactor) reached 99.5% after 15 min, and the total organic carbon (TOC) removal of PG solution reached 21.2% after 2 h. Meanwhile, the results of control experiments indicated that the PG degradation efficiency of CZ/O 3 system was higher than that of O 3 alone system. The CZ catalyst was characterized by TEM, SEM-EDS, XRD, BET and XPS. The results showed that the CZ catalyst retained the high mesopore framework of natural zeolite. Cerium oxide was successfully supported on the zeolite. Then, radical scavenging tests revealed that hydroxyl radical ( OH) and superoxide radical ( O 2 − ) were the dominant reactive oxygen species (ROS) for PG degradation. Furthermore, fifteen species of PG degradation intermediates were found by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UHPLC/orbitrap-MS) and ion chromatography (IC). Also, two possible PG degradation pathways in the heterogeneous catalytic ozonation systems were proposed and the catalytic mechanism of CZ/O 3 system was analyzed in detail. Finally, the potential toxicity of the intermediates formed during the catalytic ozonation was examined, the toxicity decreased after 60 min. In brief, this study would provide a low-cost and efficient method for removing high concentration PG in solution.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2020
·Vol. 67
·期 9
·1591-1601
·
IF 1.554
Zheng Zhaoyu,Xue Ping,Shi Keren,Zhang Weiwei,Hu Chunmiao,Li Peng
The novel di-functional magnetic nanoflowers (DMNF) which had both epoxy groups and hydrophilic catechol as well as phthaloquinone groups capable of covalently coupling of penicillin G acylase (PGA) were characterized by scanning electron microscopy, transmission electron microscope (TEM), vibrating sample magnetometer, N 2 adsorption, and so on. The studies showed that DMNF possessed “hierarchical petal” structure of nanosheets had specific saturation magnetization of 39.7 emu/g and average pore diameter of 25.4 nm as well as specific surface area of 17.28 m 2 /g. For hydrolysis of penicillin G potassium catalyzed by the PGA immobilized on DMNF with enzyme loading of 106 mg/g-support, its apparent activity reached 2,667 U/g, which benefited from the “hierarchical petal” and large pore structure of the magnetic DMNF leading to high enzyme loading and fast diffusion of substrate molecules to the immobilized PGA to reaction. The apparent activity of the immobilized PGA could keep 2,408 U/g (above 90% of its initial activity) after repeating use for 10 cycles. The magnetic immobilized PGA exhibited excellent operational stability due to covalently coupling of the enzyme molecules between the support by covalent interaction of the amino groups of PGA and the reactive groups of epoxy, catechol, and phthaloquinone groups on DMNF. Furthermore, the PGA displayed good acid and alkaline resistance as well as thermal stability by immobilization using DMNF.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
高影响因子
GOLD BULLETIN
·
2023
·Vol. 56
·期 1
·1-7
·
IF 2.2
Wang Tao,Liu Qian,Shi Minshan,Chang Rong,Tang Jun,He Yalan,Wu Dongling
The high enhancement activity of litchi-like gold nanoparticles (Au NPs) is prepared in deep eutectic solvent (DES), and the prepared Au NPs as SERS active substrate exhibits excellent sensitivity when rhodamine 6G (R6G) is used as a probe molecule for detection. The enhancement factor (EF) is calculated to be about 1.8 × 10 12 , and the minimum detected concentration is outstanding in R6G aqueous solution at 10 −12 M. In addition, the microtrace determination of penicillin G sodium (PG) in camel milk powder produced in Xinjiang was successfully achieved by using the prepared Au NPs. This work provides an environmentally friendly, simple, and rapid method to prepare efficient and sensitive surface-enhanced Raman scattering (SERS) materials. Meanwhile, it also uncovers a new possibility for the development of various nanoparticles with SERS prepared using DES as reactive solutions. Graphical abstract
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2024
·Vol. 19
·期 1
·e0297149
·
IF 2.9
Zhixiang Lv,Zhou Wang,Shaobo Wu,Xiang Yu
With the emergence of penicillin resistance, the development of novel antibiotics has become an urgent necessity. Semi-synthetic penicillin has emerged as a promising alternative to traditional penicillin. The demand for the crucial intermediate, 6-aminopicillanic acid (6-APA), is on the rise. Enzyme catalysis is the primary method employed for its production. However, due to certain limitations, the strategy of enzyme immobilization has also gained prominence. The magnetic Ni 0.4 Cu 0.5 Zn 0.1 Fe 2 O 4 nanoparticles were successfully prepared by a rapid-combustion method. Sodium silicate was used to modify the surface of the Ni 0.4 Cu 0.5 Zn 0.1 Fe 2 O 4 nanoparticles to obtain silica-coated nanoparticles (Ni 0.4 Cu 0.5 Zn 0.1 Fe 2 O 4 -SiO 2 ). Subsequently, in order to better crosslink PGA, the nanoparticles were modified again with glutaraldehyde to obtain glutaraldehyde crosslinked Ni 0.4 Cu 0.5 Zn 0.1 Fe 2 O 4 -SiO 2 -GA nanoparticles which could immobilize the PGA. The structure of the PGA protein was analyzed by the PyMol program and the immobilization strategy was determined. The conditions of PGA immobilization were investigated, including immobilization time and PGA concentration. Finally, the enzymological properties of the immobilized and free PGA were compared. The optimum catalytic pH of immobilized and free PGA was 8.0, and the optimum catalytic temperature of immobilized PGA was 50°C, 5°C higher than that of free PGA. Immobilized PGA in a certain pH and temperature range showed better catalytic stability. V max and K m of immobilized PGA were 0.3727 μmol·min -1 and 0.0436 mol·L -1 , and the corresponding free PGA were 0.7325 μmol·min -1 and 0.0227 mol·L -1 . After five cycles, the immobilized enzyme activity was still higher than 25%.
研究领域:
生物化学
·
材料科学
·
制药工程
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产品关联
DOI
PubMed
高影响因子
RADIATION PHYSICS AND CHEMISTRY
·
2017
·
IF 1.315
Libing Chu,Shuting Zhuang,Jianlong Wang
The gamma radiation induced-degradation of a β-lactam antibiotic, penicillin G was investigated in aqueous solution. Special attention was paid to the effects of the organic substances such as peptone and glucose on penicillin G degradation, which can be found in the wastewater of the factories producing antibiotics. Results showed that gamma radiation was effective to degrade and deactivate penicillin G in pure water. With the initial concentrations of 0.27 mM, 1.34 mM and 2.68 mM, a complete removal of penicillin G could be achieved at the adsorbed doses of 2.5 kGy, 10 kGy and 20 kGy, respectively. Penicilloic acid from the β-lactam ring cleavage and a series of fragment compounds such as thiazolidine and penicillic acid were identified during gamma irradiation-induced degradation of penicillin G. Addition of Fe 2+ was efficient to enhance the mineralization. The TOC removal efficiency of penicillin G was 21.7% using gamma irradiation alone at 10 kGy, which increased to 56.4% with 1.0 mM Fe 2+ addition. The gamma radiation-induced degradation of penicillin G was inhibited in the presence of peptone and glucose and the inhibitive effect increased with increasing their concentrations. The rate constant, k of the pseudo first-order kinetics decreased by 74% and 64% in the presence of 1.0 g/L of peptone and glucose, respectively, and by 96% and 89% in the presence of 10 g/L of peptone and glucose, respectively. The ratio of k / k 0 was increased by 1.3 times with H 2 O 2 addition and by 3 times with Fe 2+ addition, in the presence of 10 g/L of glucose. Adding Fe 2+ was effective to improve the ionizing radiation induced degradation of penicillin G antibiotic in the glucose-containing wastewater.
研究领域:
环境科学
·
辐射化学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）