INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2021
·Vol. 56
·期 12
·6591-6600
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IF 3.713
Jiali Yang,Tengfei Wang,Jianbing Di,Yaping Liu,Xiaoling Hao,Yu Wang
Summary Although several studies have shown the ability of l -glutamate (glutamate) to mitigate the stress imposed by pathogens, the underlying mechanism is still in its infancy. To gain further knowledge, this study focussed on the effect of glutamate on primary nitrogen and carbon metabolisms during apple- Penicillium expansum interaction. The obtained result showed that glutamate could effectively restrict blue mould rot development in apples, but had no direct impact on fungal growth in vitro . The application of glutamate increased the level of nitrogen in apples, resulting in a disrupted balance of carbon and nitrogen. Consistently, the key enzymes glutamine synthetase (GS) and glutamate synthase (GOGAT) involved in the GS/GOGAT cycle, and the deaminating activity of glutamate dehydrogenase (GDH), forming 2-oxoglutarate and ammonium from glutamate, were promptly stimulated by glutamate. Interestingly, glutamate led to considerable consumption of the tricarboxylic acid (TCA) cycle intermediates, such as isocitric acid and citric acid, accompanied by the enhancement of malate dehydrogenase and succinate dehydrogenase activities. Collectively, exogenous application of glutamate might confer blue mould resistance in apples, at least in part, by redirecting host’s primary nitrogen and carbon metabolisms, such as the activation of the GS/GOGAT cycle, deaminating activity of GDH and the TCA cycle.
研究领域:
植物病理学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF STORED PRODUCTS RESEARCH
·
2024
·
IF 2.7
Lingling Li,Chunhua Wu,Qian Chen,Zhengjun Shi,Kaimeng Xu,Yanfei Niu,Xiaoping Rao
The presence of chemical pesticides has an impact on human health and there is an urgent need for the development of green antifungal alternatives. This study investigated the freshness application of dehydroabietic acid-modified chitosan product (CSDA) mixed with three essential oil (litsea cubeba oil (LCO), wintergreen essential oil (WEO), cinnamon essential oil (CEO)). The antifungal activity of modified chitosan/essential oils was tested by the punching method and the antifungal mechanism was investigated by the transmission electron microscope (TEM). The results showed that fungal inhibition rate of CSDA-WEO on Penicillium digitatum reached 100% at a concentration of 0.05 μL/mL and on Penicillium italicum reached 100% at a concentration of 1 μL/mL. Both molds were not well inhibited by CSDA-LCO and CSDA-CEO. CSDA-WEO was able to affect the cell walls and energy metabolism of the molds, resulting in nutrient loss and death. Fruits were preserved with 0.1 mg/g CSDA and 1 μL/mL WEO. CSDA-WEO maintained the antioxidant activity of citrus , inhibited respiration and water loss, and extended shelf life up to 24 days. CSDA-WEO extended the shelf life of longan to 18 days and kiwi slices to 6 days. CSDA-WEO is expected to serve as an alternative to chemical fungicides and shows promise as a broad-spectrum fruit and vegetable preservative.
研究领域:
食品科学
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微生物学
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植物病理学
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农业化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2016
·
IF 2.429
Jinwei Zhang,Zhangwei Han,Bo Teng,Wuyong Chen
For getting a clear and detail process of chromium tanned leather biodeteriorated with Penicillium sp. as a reference for future research and providing a cleaner chromium containing leather waste disposal method. The chromium tanned leather (CTL) and chromium tanned hide powder (CTHP) were biodeteriorated with the Penicillium . The CTL was biodeteriorated at first to verify the biodeterioration effect, and then the changes of the CTHP structure and thermal properties after the biodeterioration were analyzed by Scanning Electron Microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), Thermal Gravimetric Analyzer (TG) and Differential Scanning Calorimeter (DSC). The deterioration degree of the CTHP was characterized by the contents of hydroxyproline and total nitrogen in the filtrate, the Penicillium number was counted too during the process. The results showed that the CTL could be biodeteriorated with the Penicillium as shrinkage temperature and mass decreasing even though the biodeterioration degree was not ideal enough. After the process of CTHP biodeteriorated with the Penicillium , the surface morphology and structure of the CTHP were damaged. The thermal resistance and thermal denaturing temperature reduced which showed the detanning effect happened in the biodeterioration process. With the increasing of biodeterioration time, the hydroxyproline and total nitrogen in the filtrate increased. The Penicillium number maximized at 96 h and protease was produced in the process with enzymatic activity reaching to 9.23U mL −1 . These phenomena demonstrated the Penicillium grew through digesting chromium tanned leather and produced metabolites. To sum up, the biodeterioration process of chromium tanned leather with Penicillium was a synergistic effect of chromium detanning and the Penicillium growth.
研究领域:
微生物学
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环境科学
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皮革化学
✓ 已验证
DOI
高影响因子
FUNGAL GENETICS AND BIOLOGY
·
2013
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IF 3.263
Yibo Hu,Guodong Liu,Zhonghai Li,Yuqi Qin,Yinbo Qu,Xin Song
Heterotrimeric G proteins (G proteins) have been extensively investigated for their regulatory functions in morphogenesis and development in filamentous fungi. In addition, G proteins were also shown to be involved in the regulation of cellulase expression in some fungi. Here, we report the different regulatory effects of PGA3, a group III G protein α subunit, on the expressions of amylases and cellulases in Penicillium decumbens . Deletion of pga3 resulted in impaired amylase production and significantly decreased transcription of the major amylase gene amy15A . Supplementation of exogenous cAMP or its analog dibutyryl-cAMP restored amylase production in Δ pga3 strain, suggesting an essential role of PGA3 in amylase synthesis via controlling cAMP level. On the other hand, the transcription of major cellulase gene cel7A-2 increased, nevertheless cellulase activity in the medium was not affected, in Δ pga3 . The above regulatory effects of PGA3 are carbon source-independent, and are achieved, at least, by cAMP-mediated regulation of the expression level of transcription factor AmyR. The functions of PGA3 revealed by gene deletion were partially supported by the analysis of the mutant carrying dominantly-activated PGA3. The results provided new insights into the understanding of the physiological functions of G protein-cAMP pathway in filamentous fungi.
研究领域:
分子生物学
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微生物学
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真菌遗传学
✓ 已验证
DOI
PubMed
高影响因子
Systems Microbiology and Biomanufacturing
·
2022
·Vol. 2
·期 4
·634-642
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IF 0
Zhou Hengrui,Gao Song,Zeng Weizhu,Deng Zhenshan,Zhou Jingwen
Citrinin is a common mycotoxin produced by different fungi. Although considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs, few methods are effective, owing to potential safety concerns and technical limitations. In this study, a blue fungus that produces a yellow pigment was isolated from Ziziphus jujuba . The strain was then identified as Penicillium citrinum and named HR-087. Preparative liquid chromatography was used to extract the yellow pigment. Both LC–MS and NMR assays showed that it was citrinin. To enhance the citrinin production of this strain, the culture medium was optimized through orthogonal experiments, as well as batch and fed-batch process. The final titer and productivity of citrinin reached 9.62 g/L and 0.1 g/(L × h), respectively. The high titer achieved indicates the possibility of large-scale production of high purity citrinin for low-cost supply for both academic and diagnostic analysis standard.
研究领域:
微生物学
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生物技术
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天然产物化学
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发酵工程
✓ 已验证
DOI
Food Bioscience
·
2019
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IF 3.22
Weilu Guo,Mouming Zhao,Qingjin Chen,Li Huang,Yan Mao,Ning Xia,Jianwen Teng,Baoyao Wei
The ability of a strain to produce citrinin was analysed in the growth inhibition zone using HPLC and UHPLC–MS. A total of 12 Penicillium citrinum strains isolated from Liupao tea were inoculated onto tea leaves and their capability for citrinin production were studied using different water content (15–45%) and temperatures (15–40 °C). The results provided a theoretical base for the safe manufacture of Liupao tea. Five strains of P. citrinum produced a clear growth inhibition zone with diameters of 11–22 mm. The citrinin contents in the liquid cultures of the 12 strains of P. citrinum ranged from 0.03 to 14.5 μg/mL. The citrinin contents of the teas with initial water content of 15 and 25% and those inoculated with P. citrinum were below the limit of detection during the fermentation. The citrinin content of the teas with initial water contents of 35 and 45% at 25 °C increased rapidly at first and then decreased during the fermentation. Similarly, the citrinin contents of the teas at 15 and 25 °C rapidly increased and then decreased. Meanwhile, P . citrinum was unable to produce citrinin at 35 and 40 °C. The optimum conditions were 45% water and 25 °C, generating the highest citrinin yield of 181 ng/g, which was higher than the limit of 50 ng/g citrinin in red yeast rice that can cause harm to human health. The highest citrinin yield in tea fermented for 50 days was 34.8 ng/g for stored teas with proper conditions. Therefore, storing teas with a low water content and temperature can reduce the content of citrinin.
研究领域:
食品科学
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微生物学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
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2021
·
IF 7.086
Rui Qu,Huijie Hou,Keke Xiao,Bingchuan Liu,Sha Liang,Jingping Hu,Shijie Bian,Jiakuan Yang
The evaluation of ecological risks of contaminant mixtures to organisms is very challenging due to the non-linear response of organisms to each component, especially under the co-existence of both stimulators and inhibitors. Whether the stimulatory effect can reduce or even offset the inhibitory effect would be critical to the risk assessment and the treatment measures of mixed pollutants. Here, the combined toxicity of sodium fluoride (NaF), a stimulator with stimulation rate >100%, and six compounds that cannot induce hormesis (four ionic liquids (ILs) and two pesticides) were studied. The time-dependent toxicity of each toxicant on Vibrio qinghaiensis sp.-Q67 was investigated at 0.25, 2, 4, 6, 8, 10 and 12 h. Results showed that four ILs and two pesticides failed to induce hormesis, while NaF induced hormesis from 2 to 6 h and induced stimulation only after 6 h and reached its maximum (650%) at 12 h. All mixture rays with NaF induced hormesis at different times. In the four NaF-IL mixture systems, the absolute value of maximum stimulation demonstrated an upwards and then a downwards trend with the increasing of mixture ratio of IL. In two NaF-pesticide systems, the maximum stimulation effect declined with the increasing of the mixture ratio of pesticide. The toxicities of the mixture were successfully predicted by the improved inverse distance weighted interpolation, which are not able to be predicted by the commonly used concentration addition or independent action models. This paper shed lights on evaluating the hormesis of mixtures and the ecological risk of fluoride.
研究领域:
生态毒理学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Haichun Zeng,Jiatong Zeng,Beilin Meng,Jianmei Peng,Li Rao
Geraniol, an acyclic monoterpenoid of substantial value extracted from the essential oils of various aromatic plants, holds significant commercial and industrial importance in the realms of food, cosmetics, medicine, and bioenergy. Geraniol synthase, which is responsible for geraniol production, has been identified in only several plant species to date. Here, we present the first cloning and characterization of a geraniol synthase (PgfTPS) from Penicillium griseofulvum. This enzyme demonstrates pronounced specificity in catalyzing the conversion of geranyl diphosphate into geraniol. Moreover, through protein modeling and site-directed mutagenesis, we have identified key active-site residues crucial for the catalytic function of PgfTPS. Finally, we utilized engineered Saccharomyces cerevisiae as a host for PgfTPS expression to facilitate geraniol production. Our findings not only advance the development of efficient biocatalysts for geraniol generation but also establish a fundamental basis for further exploration into fungal monoterpene biosynthesis.
研究领域:
生物化学
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微生物学
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植物代谢工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2021
·
IF 5.537
Yi Li,Long Guo,Zhiqin Zhou
Penicillium italicum ( P. italicum ), the cause of citrus blue mould, is a pathogenic fungus that seriously affects the postharvest quality of citrus fruit and causes severe economic loss. Our previous research showed that adding limonin to eugenol nanoemulsion could improve the antifungal activity, and inhibited the growth of P. italicum on the surface of citrus fruit. Accordingly, the present study aimed to investigate the underlying mechanism of limonin-loaded eugenol emulsion (EuLm) against P. italicum based on the influence on membrane structure and intracellular substance metabolism. Results indicated that EuLm showed disruptive activity on the fungal cell membrane, mitochondrial membrane potential and the ergosterol synthesis. Meanwhile, EuLm treatment causes the intracellular substances of total lipids, cell proteins and total sugars to decrease by 56.7 %, 37.4 % and 17.4 %, respectively. GC–MS-based metabolomics showed 34 metabolites declined after EuLm treatment, including amino acids, fatty acids, carbohydrates, alcohols, and glycosides. The results suggested that EuLm interferes with fungal metabolic pathways, such as the TCA cycle, lipid metabolism and protein synthesis. Our study provides new insight into exploring the underlying mechanisms of antifungal phytochemicals against P. italicum .
研究领域:
植物化学
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微生物学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Jie Tang,Chaoran Yang,Xiaoting Qin,Jingyu Si,Xuewen Dong,Xiaobo Hu,Qiang Yu
Citrus aurantium L. (CAL) was a potential low-cost source of soluble dietary fiber (SDF). To make full use of this resource, a considerable efficiency cellulose degrading strain ( Penicillium griseofulvum ) was screened from soil humus and used in the modification of CAL-SDF. After fermentation by Penicillium griseofulvum , the structure characteristics and adsorption properties of CAL-SDF were improved. Under the optimum fermentation conditions, the yield of SDF enlarged from 5.47% to 20.0%. Scanning electron microscope (SEM) and Fourier infrared spectroscopy (FTIR) analysis showed that the microstructure became loose and porous after fermentation, unfermented SDF (U-SDF) and fermented SDF (F-SDF) had characteristic intake peaks of polysaccharide . The results of X-ray diffraction (XRD) and thermogravimetric analysis (TGA) showed that the crystallization degree (from 16.50% to 23.15%) of SDF was improved by fermentation, which increased the thermal stability of SDF. The monosaccharide composition and average molecular weight (Mw) results showed that fermentation altered the monosaccharide percentage of SDF as well as significantly reduced the molecular weight (from 576,914 to 112,977 Da). Together, these alterations reduced the water holding capacity (from 11.31 to 1.31 g/g) and oil holding capacity (from 14.24 to 12.86 g/g) of SDF, but significant ( P < 0.05) increased water solubility (from 89.35% to 92.09%), the glucose dialysis retardation index (GDRI), cholesterol adsorption capacity (CAC) and nitrite adsorption capacity (NIAC). In summary, Penicillium griseofulvum could be used as a potential strain for the modification of the CAL-SDF. Moreover, F-SDF may be used as a functional food component, which will increase CAL's value proposition.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Rumeng Li,Bo Yao,Haichun Zeng
Nerol, a linear monoterpenoid, is naturally found in essential oils of various plants and is widely used in the fragrance, food, and cosmetic industries. Nerol synthase, essential for nerol biosynthesis, has previously been identified only in plants that use NPP as the precursor. In this study, a novel fungal nerol synthase, named PgfB, was cloned and characterized from Penicillium griseofulvum. In vitro enzymatic assays showed that PgfB could directly convert the substrate GPP into nerol. Furthermore, the successful expression of PgfB and its homologous protein in Saccharomyces cerevisiae resulted in the heterologous production of nerol. Finally, crucial amino acid residues for PgfB’s catalytic activity were identified through site-directed mutagenesis. This research broadens our understanding of fungal monoterpene synthases and presents precious gene resources for the industrial production of nerol.
研究领域:
真菌学
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生物化学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2010
·
IF 1.412
Shuyan Zang,Bin Lian,Juan Wang,Yanjie Yang
To explore biodegradation of 2-naphthol and its metabolites accumulated in wastewater treatment, a series of bio-degradation experiments were conducted. Two main metabolites of 2-naphthol, 1,2-naphthalene-diol and 1,2-naphthoquinone, were identified by high-performance liquid chromatography with standards. Combining fungus Aspergillus niger with bacterium Bacillus subtilis in the treatment enhanced 2-naphthol degradation efficiency, lowered the accumulation of the two toxic metabolites. There were two main phases during the degradation process by the kinetic analysis: 2-naphthol was first partly degraded by the fungus, producing labile and easily accumulated metabolites, and then the metabolites were mainly degraded by the bacterium, attested by the degradation processes of 1,2-naphthalene-diol and 1,2-naphthoquinone as sole source of carbon and energy. Sodium succinate, as a co-metabolic substrate, was the most suitable compound for the continuous degradation. The optimum concentration of 2-naphthol was 50 mg/L. The overall 2-naphthol degradation rate was 92%, and the COD Cr removal rate was 80% on day 10. These results indicated that high degradation rate of 2-naphthol should not be considered as the sole desirable criterion for the bioremediation of 2-naphthol-contaminated soils/wastewater.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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