Tissue Engineering Part C-Methods
·
2024
·Vol. 30
·期 3
·113-129
·
IF 2.7
Xiaoxiao Li,Weikang Zhao,Dandan Zhou,Pei Li,Chen Zhao,Qiang Zhou,Yiyang Wang
The decellularized extracellular matrix (ECM) of cartilage is a widely used natural bioscaffold for constructing tissue-engineered cartilage due to its good biocompatibility and regeneration properties. However, current decellularization methods for accessing decellularized cartilaginous tissues require multiple steps and a relatively long duration to produce decellularized cartilage. In addition, most decellularization strategies lead to damage of the microstructure and loss of functional components of the cartilaginous matrix. In this study, a novel decellularization strategy based on a hydrostatic pressure (HP) bioreactor was introduced, which aimed to improve the efficiency of producing integral decellularized cartilage pieces by combining physical and chemical decellularization methods in a perfusing manner. Two types of cartilaginous tissues, auricular cartilage (AC) and nucleus pulposus (NP) fibrocartilage, were selected for comparison of the effects of ordinary, positive, and negative HP-based decellularization according to the cell clearance ratio, microstructural changes, ECM components, and mechanical properties. The results indicated that applying positive HP improved the efficiency of producing decellularized AC, but no significant differences in decellularization efficiency were found between the ordinary and negative HP-treated groups. However, compared with the ordinary HP treatment, the application of the positive or negative HP did not affect the efficiency of decellularized NP productions. Moreover, neither positive nor negative HP influenced the preservation of the microstructure and components of the AC matrix. However, applying negative HP disarranged the fibril distribution of the NP matrix and reduced glycosaminoglycans and collagen type II contents, two essential ECM components. In addition, the positive HP was beneficial for maintaining the mechanical properties of decellularized cartilage. The recellularization experiments also verified the good biocompatibility of the decellularized cartilage produced by the present bioreactor-based decellularization method under positive HP. Overall, applying positive HP-based decellularization resulted in a superior effect on the production of close-to-natural scaffolds for cartilage tissue engineering.Impact statementIn this study, we successfully constructed a novel hydrostatic pressure (HP) bioreactor and used this equipment to produce decellularized cartilage by combining physical and chemical decellularization methods in a perfusing manner. We found that positive HP-based decellularization could improve the production efficiency of integral decellularized cartilage pieces and promote the maintenance of matrix components and mechanical properties. This new decellularization strategy exhibited a superior effect in the production of close-to-natural scaffolds and positively impacts cartilage tissue engineering.
研究领域:
组织工程
·
生物医学工程
·
软骨生物学
·
机械工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 15
·2190
·
IF 5.561
Yaqiong Zhang,Zhi-Hong Zhang,Ronghai He,Riyi Xu,Lei Zhang,Xianli Gao
Using high hydrostatic pressure (HHP) to treat liquid foods can improve their aroma; however, no information about the effects of HHP on soy sauce aroma has yet been reported. The effects of HHP on the aroma of soy sauce fermented for 30 d were investigated using quantitative descriptive analysis (QDA), SPME–GC–olfactometry/MS, hierarchical cluster analysis (HCA) and principal component analysis (PCA). Results showed that the pressure used during HHP treatment had a greater influence on soy sauce aroma than the duration of HHP. Compared to the control, soy sauce that was treated with HHP at 400 MPa for 30 min (HHP400–30) obtained the highest sensory score (33% higher) by increasing its sour (7%), malty (9%), floral (27%) and caramel-like (47%) aromas, while decreasing its alcoholic (6%), fruity (6%) and smoky (12%) aromas; moreover, the aroma of HHP400–30 soy sauce was comparable with that of soy sauce fermented for 180 d. Further investigation demonstrated that HHP (400 MPa/30 min) enhanced the OAVs of compounds with sour (19%), malty (37%), floral (37%), caramel-like (49%) and other aromas (118%), and lowered the OAVs of compounds with alcoholic (5%), fruity (12%) and smoky (17%) aromas. These results were consistent with the results of the QDA. HHP treatment positively regulated the Maillard, oxidation and hydrolysis reactions in raw soy sauce, which resulted in the improvement and accelerated formation of raw soy sauce aroma. HHP was capable of simultaneously improving raw soy sauce aroma while accelerating its aroma formation, and this could treatment become a new alternative process involved in the production of high-quality soy sauce.Keywords:soy sauce;high hydrostatic pressure;aroma compound;Aspergillus oryzae;soybean
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2017
·Vol. 12
·期 10
·1056-1061
·
IF 0.497
Li Shengbin,Hu Bingxu,Zhang Fan
In this paper, we report the fabrication and characterizations of polyvinylidene fluoride (PVDF)/chitosan and PVDF/polymeric methyl methacrylate (PMMA)/titanium oxide (TiO2) materials membranes for waterproofing applications. The detailed results revealed that with the increase
of chitosan proportion, the anti-hydrostatic pressure value of PVDF/chitosan raised first and then declined. When the proportion of PVDF and chitosan reaches 92:8, the anti-hydrostatic pressure value reaches to 5437 Pa, however, when chitosan exceeds the value, its hydrophobicity was reduced.
In addition, the fibrous membrane prepared from PVDF/PMMA/TiO2 was adopted to make a thermal treatment and 6% TiO2 nanoparticles were added to improve the intensity and anti-water pressure of the fibers. The detailed experiments revealed that when the reaction temperature
reach to 50 °C, the anti-hydrostatic pressure of fibrous membrane is far better than fibrous membrane prepared from PVDF/chitosan. Thus, the results confirmed that by adding 6% TiO2 nanoparticles improves the anti-hydrostatic pressure of fibrous membrane. The observed results
shows that chitosan and TiO2 blended with PVDF are promising material for waterproofing applications.
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2018
·
IF 3.52
Qiang Xia,Yunfei Li
This investigation aimed to examine the effects of a novel processing pattern, combining high hydrostatic pressure (HHP) with germination, on the embryo growth and physicochemical characteristics of wholegrain brown rice (WBR). WBR grains were firstly subjected to mild HHP stress (30–90 MPa/5 min) and then incubated at 37 °C for 52 h for obtaining germinated samples (GBR). The results showed that HHP shock resulted in a delayed embryo growth of WBR grains, maintaining acceptable sprouting rates ranging from 65% to 76% when germination was finished. The contents of gama-aminobutyric acid in GBR were greatly increased responding to HHP stress, showing pressure intensities dependent. Total digestible and resistant starch contents in samples stressed at 60/90 MPa were decreased, mainly associated with high pressure-induced amorphization as revealed by SEM imaging and FTIR , which promoted starch hydrolysis during germination. Besides, the levels of zinc and iron were influenced by HHP pretreatments due to the high pressure-mediated degradation behavior for phytic acids. The storability of HHP-stressed GBR grains was significantly enhanced through reducing free fatty acids formation and maintaining color stability during a storage testing. These results obtained from the current work demonstrated that mild HHP stress pretreatment prior to germination process could be used as a promising strategy to modulate certain physicochemical characteristics of WBR products.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Melani Purnika Mudugamuwa Arachchige,Taihua Mu,Mengmei Ma
Novel pectin derived from sweet potato residue was modified by high hydrostatic pressure (HHP)-assisted pectinase and then used for Pb 2+ removal from aqueous solutions. The removal characteristics and mechanisms were also investigated. Results showed that modified sweet potato pectin exhibited greater adsorption performances for Pb 2+ than that of natural ones, and showed excellent eco-friendly properties and good potential for adsorption of some other heavy metals (such as Cu 2+ ). The adsorption curves were much more conformed to Langmuir model, and the highest capacity for Pb 2+ adsorption was 263.15 mg/g with 1.00% pectin at pH 7. Chemical adsorption process of pectin for Pb 2+ absorption involved O-containing functional groups (O–H, COO-), cation exchange, and along with electrostatic interactions. Overall, the results in this study indicated that sweet potato pectin modified with HHP-assisted pectinase had the potential to become an environmentally friendly coagulant-flocculant agent for the heavy metal adsorption, especially for Pb 2+ .
研究领域:
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2019
·Vol. 3
·期 6
·1052-1061
·
IF 0
Tingting Lin,Xing Su,Kai Wang,Minjie Li,Hongwei Guo,Lulu Liu,Bo Zou,Yu-Mo Zhang,Yifei Liu,Sean Xiao-An Zhang
Stimuli-responsive fluorescent materials which can change their optical signal outputs upon changes in ambient physical or chemical parameters exhibit great application potential for comprehensive environmental monitoring. In this article, a new aggregation-induced emission fluorescent switch, 1',3′,3′-trimethyl-5′-(4-(1,2,2-triphenylvinyl)phenyl)spiro[chromene-2,2′-indoline]-6-carbonitrile (TPE-Sp-CN), was synthesized and applied in the quantitative detection of three parameters, namely pH value, sulfite anion and hydrostatic pressure, with different molecular states. According to the analysis of the optical spectra, TPE-Sp-CN can distinguish water pH values from 0 to 4, the detection limit of sulfite anion is 1.04 ppm, and the pressure sensing range is 1 atm to 6 GPa with the sensing sensitivity of a 9.876 nm GPa−1 shift in the PL spectrum. Furthermore, TPE-Sp-CN can quantitatively sense all three parameters with good linear relationships, which is rarely reported. Also, it is promising to use TPE-Sp-CN as an indicator to monitor the environment or disasters that are related to the above parameters, such as harmful geological activities.
研究领域:
材料科学
·
环境监测
·
化学传感
✓ 已验证
产品关联
DOI
FOOD CHEMISTRY
·
2022
·
IF 9.231
Sinan Zhang,Zhenhong Zheng,Chuyao Zheng,Yadong Zhao,Zhuo Jiang
The mechanism of the high hydrostatic pressure (HHP) effect on horseradish peroxidase (HRP) is still unclear. The activity, thermal stability and structural changes of HRP after HHP treatments were studied in this work. Compared with the untreated sample, the enzyme activity reduces by 36% after 800 MPa processing. The results indicated that the conformation of the enzyme active center changes under pressure. Furthermore, HHP also changes the conformation of disulfide bonds and some secondary structures in HRP. These structural and conformational changes induce decreased activity. In addition, differential thermal scanning (DSC) results showed that the thermal denaturation temperature decreased from 103.74 °C to 85.78 °C after pressure treatment, suggesting HRP molecules formed large aggregates after pressure treatment. In this study, the interaction mechanism between pressure and enzyme was studied as well, and the results can provide some guidance for the application of HHP technology in fruit and vegetable products processing.
研究领域:
食品科学
·
酶学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Xiangxiang Sun,Ahmed S.M. Saleh,Yifan Lu,Zhuangzhuang Sun,Xiuyun Zhang,Xiangzhen Ge,Huishan Shen,Xiuzhu Yu,Wenhao Li
In this study, proso millet starch was isolated and subjected to treatment with ultra-high pressure (UHP), cold plasma (CP), or their combination to modify its functional properties. The changes in structural, physicochemical, and digestive properties of proso millet starch after these treatments were investigated. The proso millet native starch granules showed irregular and polygonal shapes with a smooth surface. Treatments with CP or UHP at low pressures did not change the morphological properties or crystalline structure type of proso millet starch granules, while the treatment with UHP at 600 MPa and CP resulted in a complete gelatinization of starch. Also, UHP treatment at high pressure, followed by CP treatment, destroyed the partial crystalline region and reduced the short-range order of proso millet starch. Besides, a combination of UHP and CP treatment promoted the depolymerization of long chains in proso millet starch. Moreover, the combined treatments could enhance the resistance to high temperature and shearing and improve the pasting stability of starch. Furthermore, the combined treatment could increase the slowly digestible starch content. Therefore, the combination of UHP and CP treatment can be suggested for modifying the functional properties of proso millet starch and promoting its industrial applications.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Yang He,Liankui Wen,Hansong Yu,Fei Zheng,Zhitong Wang,Xuanwei Xu,Hao Zhang,Yong Cao,Bixiang Wang,Baiji Chu,Jianyu Hao
Natural anthocyanins are safer and nutritious as compared to synthetic pigments; however, their stability is poor. They can produce spontaneous copigmentation with organic acids, leading to the improvement of colour stability, albeit slowly. Box–Behnken experimental design was used to elucidate the mechanism of copigmentation between Vitis amurensis Rupr anthocyanins (0.1 mg/mL) and organic acids (0.87 mg/mL, ferulic acid: d -gluconic acid:caffeic acid:vanillic acid = 1.5:2.5:2.5:0.5, w/w/w/w) promoted by high hydrostatic pressure (HHP; 300 MPa, 2 min). The copigmentation effect and antioxidant activity of anthocyanins were also evaluated. The structure of anthocyanins was analysed using ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy, high-performance liquid chromatography, and mass spectrometry. The results of HHP copigmentation showed that the following anthocyanins were newly formed—delphinidin-3- O -catechol, petunidin-3- O -catechol, delphinidin-4-vinyl-catechol, petunidin-3- O -guaiacol, malvidin-4-vinyl-guaiacol, cyanidin-3- O -(6″- O -caffeoyl)-glucoside, peonidin-3- O -(6″- O -caffeoyl)-glucoside, delphinidin-3- O -(6″- O -caffeoyl)-glucoside, malvidin-3- O -glucoside-4-vinyl-guaiacol, and malvidin-3- O -(6″- O -feruloyl)-glucoside—owing to appropriate modifications that increased the copigmentation rate (R = 42.12%), photo-thermal stability (R > 45%), and potential antioxidant activities expressed in vivo ( p < 0.01 vs. Model Group).
研究领域:
食品科学
·
化学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Qiang Xia,Liping Wang,Yunfei Li
This investigation aims to evaluate the effects of high hydrostatic pressure (HHP) applied prior to germination on functionality and quality of wholegrain-germinated brown rice (GBR). Wholegrain brown rice (WBR) were firstly stressed by HHP treatments (50–350 MPa/20 min), and then incubated at 37 °C to obtain GBR grains after a 2-day soaking period. Gama-aminobutyric acid contents significantly depended on the pressure applied, showing 25% increment in 50 MPa-stressed grains compared to the control. HHP shock led to significantly improved in vitro starch digestibility, which was related to the transformation of crystalline starch granules into amorphous form as consistently revealed by scanning electron microscope imaging and Fourier-transform infrared spectroscopy. HHP-pretreated samples achieved markedly enhanced storability by influencing kinetic curves of lipid hydrolysis and oxidation. These results suggested that metabolic response to HHP during germination could significantly improve functional and quality characteristics of WBR products.
研究领域:
食品科学
·
营养学
·
农业工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TISSUE & CELL
·
2022
·
IF 2.586
Yuan Xu,Jian He,Jun He
Purpose The treatment of intervertebral disc degeneration is limited, cyanidin can protect chondrocytes from degeneration. This research investigated the effect of cyanidin on human nucleus pulposus cells (HNPC) metabolism and its mechanism. Methods HNPC were treated with cyanidin, XAV-939 (inhibitor of Wnt/β-catenin signaling) and SKL2001 (activator of Wnt/β-catenin signaling), and pressurized at 1, 3, and 30 atm. Quantitative real-time PCR and Western blot were used to assess the expressions of matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 (MMP-13), Collagen-II, Aggrecan , Wnt-3a and β-catenin in treated HNPC. Cell counting kit-8 was used to detect the HNPC cell viability . Radioisotope incorporation method was used to assess the proteoglycan synthesis rate of HNPC. The level of cell matrix was detected by toluidine blue staining. Results Proper hydrostatic pressure of 3 atm could elevate cell viability, proteoglycan synthesis and the level of cell matrix of HNPC, while high hydrostatic pressure of 30 atm reduced the above effects. Cyanidin and XAV-939 reversed the promoting effect of 30 atm pressure on Wnt/β-catenin signaling pathway and the inhibiting effect on cell viability, proteoglycan synthesis and the level of cell matrix. Subsequently, SKL2001 further reversed the function of cyanidin on HNPC. Conclusion Cyanidin attenuated the high hydrostatic pressure-induced degradation of cellular matrix of HNPC via blocking the Wnt/β-catenin signaling pathway. Our findings in this research provided a basis for in vitro experiment of cyanidin and a theoretical fundament on this disease.
研究领域:
骨科
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Shenpeng Hu,Gaihuan Ren,Yunhe Zhang,Yang Liu,Weijie Zhang,Wen Sun,Dongxiang Li,Zhenhua Cui,Wenjing Yu,Dongxu Lu,Jianxin He
High-performance waterproof and breathable nanofiber membranes are in urgent demand across various markets; however, their production typically relies on environmentally harmful fluorinated compounds or intricate manufacturing processes. In this study, we utilized fluorine-free polyurethane (PU) and stearic acid (SA) as raw materials, with dimethylformamide (DMF) employed as a pure, single solvent. A simple one-step electrospinning technique was applied to fabricate superhydrophobic PU/SA nanofiber membranes exhibiting exceptional waterproofness and breathability. This approach eliminates the need for hazardous fluorine-containing compounds while ensuring material stability through a pure solvent system and a streamlined one-step electrospinning process. Incorporating hydrophobic SA endows the PU/SA nanofiber membrane with a superhydrophobic nature, characterized by a water contact angle of 150°. By systematically optimizing the SA content within the PU/SA nanofiber membranes, the resulting membrane exhibits remarkable waterproofness, with an ultrahigh hydrostatic pressure resistance of 102.57 kPa, alongside excellent breathability, as indicated by a water vapor transmission (WVT) rate of 5.034 kg m–2 d–1. The PU/SA nanofiber membrane also has good air permeability (22.06 mm s–1) and robust mechanical properties, including a tensile strength of 7.17 MPa and an elongation at break of 74.80%. Moreover, the PU/SA nanofiber membrane demonstrates excellent antifouling and self-cleaning properties. The PU/SA nanofiber membrane developed in this study, with its superior waterproof and breathable performance, holds significant potential for applications in outdoor apparel.
研究领域:
材料科学
·
纺织工程
·
高分子化学
·
环境友好材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）