CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Jingjiu Mu,Lei Wang,Fan Wang,Weihong Liao,Hailing Dai,Yajing Yang,Yanjun Jiang,Lijuan Liu,Xiao Men,Haibo Zhang
Bioconversion can efficiently funnel the complex component of biomass into desired products, and improving the utilization efficiency of the bulk biomass has significant implications. Many mature processes have been established through the combination of pretreatment strategies and adapted strains, which focus solely on the lignocellulosic part of biomass. However, few processes have been designed for bioconversion of the full biomass component. In this study, a whole process design strategy was validated via converting full corn stover into single cell protein (SCP) and blue pigment indigoidine, respectively. The corresponding reduction in CO 2 emissions was assessed using life cycle assessment (LCA). By optimizing alkali treatment conditions, the depolymerization of full corn stover was streamlined from a traditional 12-step process to a more efficient 4-step One-Pot Process, incorporating in-situ lignin depolymerization. The optimized One-Pot Process using 0.1 g/g NaOH at 100 °C depolymerized full components of corn stover, and provided a balanced nutrition profile. The selected robust strain Rhodosporidium toruloides converted the components of depolymerized full corn stover to higher SCP and indigoidine compared to equivalent glucose, with the SCP and indigoidine yield reaching 208 g/kg and 72 g/kg, respectively. Furthermore, the LCA results demonstrated that the One-Pot Process reduced CO 2 emissions by 18 % and 27 % compared to traditional processes for SCP and indigoidine production, respectively. This study suggests a systematic design of the whole process for bioconverting full biomass components into bioproducts can simplify the pretreatment operations, enhance product yield, and reduce carbon emissions.
研究领域:
生物工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Ming Zhao,Xiu-Shan Zhang,Liang-Bin Xiong,Kun Liu,Xiang-Fei Li,Yan Liu,Feng-Qing Wang
Indigoidine, as a kind of natural blue pigment, is widely used in textiles, food, and pharmaceuticals and is mainly synthesized from l-glutamine via a condensation reaction by indigoidine synthetases, most of which originates from Streptomyces species. However, due to the complex metabolic switches of Streptomyces, most of the researchers choose to overexpress indigoidine synthetases in the heterologous host to achieve high-level production of indigoidine. Considering the advantages of low-cost culture medium and simple culture conditions during the large-scale culture of Streptomyces, here, an updated regulation system derived from the Streptomyces self-sustaining system, constructed in our previous study, was established for the highly efficient production of indigoidine in Streptomyces lividans TK24. The updated system was constructed via promoter mining and σhrdB expression optimization, and this system was applied to precisely and continuously regulate the expression of indigoidine synthetase IndC derived from Streptomyces albus J1704. Finally, the engineered strain was cultured with cheap industrial glycerol as a supplementary carbon source, and 14.3 and 46.27 g/L indigoidine could be achieved in a flask and a 4 L fermentor, respectively, reaching the highest level of microbial synthesis of indigoidine. This study will lay a foundation for the industrial application of Streptomyces cell factories to produce indigoidine.
研究领域:
微生物学
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生物工程
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合成生物学
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
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IF 16.6
Tian Jinzhong,Deng Wangshuying,Zhang Ziwen,Xu Jiaqi,Yang Guiling,Zhao Guoping,Yang Sheng,Jiang Weihong,Gu Yang
Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic V. natriegens strain leads to the production of 29.0 g · L −1 indigoidine and consumption of 165.3 g · L −1 formate within 72 h, achieving a formate consumption rate of 2.3 g · L −1 · h −1 . This work provides an important microbial chassis as well as design rules to develop industrially viable microorganisms for FA biorefinery.
研究领域:
生物技术
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代谢工程
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Ling Jia,Wei Lu,Dan Hu,Min Feng,Aoming Wang,Ruolin Wang,Hao Sun,Pan Wang,Qingyou Xia,Sanyuan Ma
Synthetic biology is an eco-friendly and sustainable approach for the production of compounds, particularly used when the production processes involve toxic reagents. In this study, we used the silk gland of silkworm to produce indigoidine, a valuable natural blue pigment that cannot be synthesized naturally in animals. We genetically engineered these silkworms by integrating the indigoidine synthetase ( idgS ) gene from S. lavendulae and the PPTase ( Sfp ) gene from B. subtilis into the silkworm genome. In the resulting Blue silkworm, indigoidine was detected at a high level in the posterior silk gland (PSG), spanning all developmental stages from larvae to adults, without affecting silkworm growth or development. This synthesized indigoidine was secreted from the silk gland and subsequently stored in the fat body, with only a small fraction being excreted by the Malpighian tubule. Metabolomic analysis revealed that Blue silkworm efficiently synthesized indigoidine by upregulating l -glutamine, the precursor of indigoidine, and succinate, which is related to energy metabolism in the PSG. This study represents the first synthesis of indigoidine in an animal and therefore opens a new avenue for the biosynthesis of natural blue pigments and other valuable small molecules.
研究领域:
合成生物学
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遗传学
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生物技术
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昆虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）