Foods
·
2023
·Vol. 12
·期 5
·1049
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IF 5.2
Qiulan Liu,Xiaoqin Zou,Yang Yi,Ying Sun,Hongxun Wang,Xueyu Jiang,Kaidi Peng
To promote the functional applications of lotus root polysaccharides (LRPs), the effects of noncovalent polyphenol binding on their physicochemical properties, as well as antioxidant and immunomodulatory activities, were investigated. Ferulic acid (FA) and chlorogenic acid (CHA) were spontaneously bound to the LRP to prepare the complexes LRP-FA1, LRP-FA2, LRP-FA3, LRP-CHA1, LRP-CHA2and LRP-CHA3, and their mass ratios of polyphenol to LRP were, respectively, 121.57, 61.18, 34.79, 2359.58, 1276.71 and 545.08 mg/g. Using the physical mixture of the LRP and polyphenols as a control, the noncovalent interaction between them in the complexes was confirmed by ultraviolet and Fourier-transform infrared spectroscopy. The interaction increased their average molecular weights by 1.11~2.27 times compared to the LRP. The polyphenols enhanced the antioxidant capacity and macrophage-stimulating activity of the LRP depending on their binding amount. Particularly, the DPPH radical scavenging activity and FRAP antioxidant ability were positively related to the FA binding amount but negatively related to the CHA binding amount. The NO production of the macrophages stimulated by the LRP was inhibited by the co-incubation with free polyphenols; however, the inhibition was eliminated by the noncovalent binding. The complexes could stimulate the NO production and tumor necrosis factor-α secretion more effectively than the LRP. The noncovalent binding of polyphenols may be an innovative strategy for the structural and functional modification of natural polysaccharides.Keywords:lotus root;polysaccharide;polyphenol;noncovalent interaction;bioactivity
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Shuhua Yin,Maoping Duan,Jian Zhang,Tuo Zhang,Guanghua Zhao
BACKGROUND Myofibrillar proteins, the main contributors to the quality of meat products, are the main structural protein component of muscle and have functional properties such as the formation of a 3D protein gel network and water binding. The susceptibility of meat-derived proteins to heat-induced aggregation is the functional constraint that hinders their applications in industry, and so establishing an effective but simple method to improve their thermostability of the proteins is of great importance. RESULTS In the present study, we describe an easy approach to perform high colloidal thermostability of both paramyosin and actin by mixing them at low ionic strength. The improvement in thermal stability was found to be derived from intermolecular interactions between these two different proteins through non-covalent binding with each other. Consequently, such interactions protected each of them from thermal-induced degradation compared to individual components. Notably, this binary native protein mixture rather than single paramyosin or actin component has the ability to form protein hydrogels with a shear-thinning and reversible sol–gel transformation behavior, which is markedly different from most of reported heat-induced, denatured protein hydrogels. CONCLUSION The present study not only presents a facile and effective strategy for improvement of the thermal stability and gel properties of a binary paramyosin and actin mixture, but also enhances our understanding of how mutual interactions of protein components affect their physicochemical and functional properties. © 2023 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Agriculture and Food Research
·
2019
·
IF 0
Junmei Su,Yuan Sun,Zhenshun Li,Yang Chen,Baomiao Ding,Weiqing Sun
Lysozyme (Ly) is a good natural food preservative. However, its enzyme activity is easily influenced by food components, such as tannins. However, the specific mechanism is still unknown. In this study, tannic acid (TA) was used as model tannin to bind with Ly. The interactional properties of Ly-TA complexes were characterized with intrinsic fluorescence intensity, fourier transform infrared spectroscopy, circular dichroism and dynamic light scattering. The enzyme activities of Ly were analyzed by the lysis rate of Micrococcus lysodeikticus . The results indicated that TA can bind well with Ly through hydrophobic, hydrogen and electrostatic interactions, which induced the unfolding of Ly molecule. Consequently, the enzyme activity of Ly was inhibited for conformational changes and masking its active sites by TA, as well as the enzyme stability further declined in pH changes, thermal treatments and high salt concentrations. Our findings shed some light on the possible explanation of negative effects from tannins on the activity of Ly in plant foods.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Jinying Ma,Hanzhong Xiao,Wan Zheng,Baicun Hao,Xinman Li,Yujia Wang,Yang Shi,Xin Huang
Conventional tannin adhesives prepared by the phenol-aldehyde condensation mechanism suffer from the formaldehyde problem. Herein, we developed environmentally benign and formaldehyde-free tannin-derived non-covalent (TNC) adhesives by manipulating the multiple-hydrogen bonding interactions between tannins and poly(vinyl alcohol) (PVA). The as-prepared TNC adhesives featured exceptional interfacial compatibility due to the amphiphilic nature of tannins, which were also capable of providing high-performance non-covalent adhesion to different substrates, including wood, glass and iron. Molecular dynamics simulations combined with the hydrogen-bond breaking experiments manifested that the hydrogen-bonding interactions between tannins and PVA were essential for the TNC adhesives to accomplish high-performance adhesion. The TNC adhesives were further applied in noncovalent adhesion of xylem fibers to prepare high-performance functional materials featured with high static bending strength. Based on this strategy, a series of environmentally benign and formaldehyde-free tannin-derived adhesives were also successfully developed by using larch tannins and black wattle tannins, respectively.
研究领域:
材料科学
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胶粘剂化学
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环境友好型材料
✓ 已验证
DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2025
·
IF 2.7
Wentao Hao,Chenchen Ke,Xianxiang Pan,Tengfei Qi,Wenping Wang,Wenjuan Li,Wen Yang
Fluorescent supramolecular materials have attracted a lot of attention over the past few decades. However, those can respond to multiple stimuli are not often reported, especially for the nonconventional fluorescent materials. Here, a supramolecular material is reported that can respond to temperature/electrical heating, humidity, volatile acids/organic amines, and mechanical strain. This material is prepared by physical mixing oligomeric polyethyleneimine (o-PEI) and oligomeric polyacrylic acid (o-PAA). Although they are almost non-fluorescent, their combination produces bright fluorescence, and the emission bands shift to longer wavelengths as the amount of o-PAA increases. Calculations based on Density Functional Theory (DFT) show that there are strong noncovalent interactions between o-PEI and o-PAA, resulting in through-space conjugation and cluster-triggered emission. By studying the relationship between emission intensity, temperature, and molecular motion, the dependence of emission intensity on relaxation time is established, which will provide insight into the luminescence origin of nonconventional fluorescent materials. In addition, this material shows good self-healing ability. The luminescence, responsiveness and self-reparability are all related to the strong but reversible noncovalent bonds that link the o-PEI and o-PAA. This work not only highlights the value of supramolecular polymers, but also enriches the research of nonconventional fluorescent materials.
研究领域:
材料科学
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化学
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2020
·
IF 4.231
Linjie Zhou,Xinjie Pei,Kun Fang,Rui Zhang,Jun Fu
Incorporating sacrificial bonds into hydrogels networks has been demonstrated an efficient way to obtain ultrahigh strength , but is usually at the expense of flexibility and recoverability. This study demonstrates novel triple physically crosslinked hydrogels with very high stretchability, toughness and rapid self-recovery at ambient conditions. Physically crosslinked κ-carrageenan acts as the first network to host free radical copolymerization of polymers with Pluronic F127 diacrylate micelles . Hydrophobic association of micelles in the second network contributes to outstanding stretchability (over 1100%) and toughness (10.83 MJ/m 3 ). Further crosslinking of the second network through tridentate Fe 3+ –COO − coordination significantly enhances the fracture strength up to 2.7 MPa, and elastic modulus 1171 kPa. Systematic investigations demonstrate the key roles played by non-covalent crosslinking on energy dissipation during loadings and the damaged non-covalent networks could recover 40% toughness in 1 min at ambient conditions. These hydrogels are promising for applications as biocompatible load-bearing materials.
研究领域:
材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2017
·Vol. 5
·期 46
·24493-24501
·
IF 8.867
Hao Wu,Zhimin Chen,Jialin Zhang,Feng Wu,Chunying He,Yiqun Wu,Zhiyu Ren
The development of innovative methods for the integration of carbon nanotubes (CNTs) and polyaniline (PANI) that preserve their exceptional quality, while robustly enriching their gas-sensing features, is still rare and remains challenging for the realization of rapid and sensitive gas detection. Herein, due to the advantages in its structure, tetra-β-carboxyphthalocyanine cobalt(II) (TcPcCo) acts not only as a sensing promoter, but also as an efficient mediator to couple multi-walled CNTs (MCNTs) with PANI, resulting in MCNT@PANI hybrids with a uniform 3D network; the planar 18π-electrons in TcPcCo allow it to closely conjugate on the surface of MCNT by π–π interactions; moreover, the outer –COOH groups in TcPcCo prompt it to link with the aniline monomers based on acid–base interactions. During the subsequent polymerization process, TcPcCo also serves as a protonic acid doped into PANI, increasing the electronic conductivity of PANI. Due to the specific selectivity of TcPcCo, high sensitivity of PANI towards NH3, and good conductivity and stability of the MCNTs, the obtained core–shell MCNT@PANI hybrids demonstrate radically enhanced NH3-sensing performance, especially in terms of response/recovery duration (∼5.0 s/12.0 s to 100 ppm NH3) and selectivity, outperforming the single components and previously reported materials at room temperature. In addition, the MCNT@1.0PANI hybrid shows a superior sensing sensitivity (140.99% to 100 ppm NH3) as well as remarkable stability over 60 days. The proposed strategy, TcPcCo-mediated non-covalent coupling of MCNT with PANI, is of potential importance towards the design and construction of ideal materials for future sensing devices, as well as many other applications.
研究领域:
材料科学
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传感器技术
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Shuohan Ma,Qingqing Zhu,Siyu Yao,Ruihao Niu,Yu Liu,Yu Qin,Yuxue Zheng,Jinhu Tian,Dandan Li,Wenjun Wang,Donghong Liu,Enbo Xu
The starch–phenolics complexes are widely fabricated as functional foods but with low phenolics retention limited by traditional liquid reaction and washing systems. In this study, ferulic acid (FA, 5%) was exogenously used in the crystalline form, and it reacted with starch in a high-solid extrusion environment, which was simultaneously controlled by thermostable α-amylase (0–252 U/g). Moderate enzymolysis (21 or 63 U/g) decreased the degree of the starch double helix and significantly increased the FA retention rate (>80%) with good melting and distribution. Although there were no significantly strong chemical bonds (with only 0.17–2.39% FA bound to starch hydrolysate), the noncovalent interactions, mainly hydrogen bonds, van der Waals forces, and electrostatic interactions, were determined by 1H NMR and molecular dynamics simulation analyses. The phased release of total FA (>50% in the stomach and ∼100% in the intestines) from bioextrudate under in vitro digestion conditions was promoted, which gives a perspective for handing large loads of FA and other phenolics based on starch carrier.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBON
·
2016
·
IF 6.198
Cheng Chen,Shihui Qiu,Mingjun Cui,Songlv Qin,Guoping Yan,Haichao Zhao,Liping Wang,Qunji Xue
Graphene(G)-based polymer nanocomposites have attracted great interest owing to their superior physicochemical properties over polymers. However, the tendency of graphene sheets to aggregate makes it difficult to achieve homogenous dispersion in polymer matrix . Herein, by utilization of poly(2-butylaniline) (P2BA) as a dispersing agent , stable dispersion of graphene in organic solvents was achieved via non-covalent π-π interactions between P2BA and graphene nanosheets . The exfoliated graphene nanosheets were then integrated with coating matrix by curing reaction of epoxy resin with P2BA functionalized graphene (P2BA-G) and amine hardener . Embedding a small percentage of well-dispersed graphene nanosheets (P2BA 0.5% -G 0.5% ) in epoxy coating remarkably improved anticorrosion performance and wear resistance properties, which was attributed to the synergistic effects of the redox catalytic capability of P2BA, high mechanical and barrier properties of well-dispersed graphene nanosheets in the epoxy matrix . The present study provides a promise strategy for development of graphene reinforced organic coatings with superior physical-mechanical properties for metal protection.
研究领域:
材料科学
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化学工程
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
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IF 6.6
Xiaoke Peng,Xiaojia Yan,David Julian McClements,Shuai Chen,Cuicui Ma,Xuebo Liu,Guoqiang Zhang,Fuguo Liu
The objective of this study was to prepare dendrobium officinale polysaccharide-epigallocatechin-3-gallate (DOP-EGCG) covalent conjugates and non-covalent complexes to enhance the antioxidant properties of the polysaccharide. The conjugates were synthesized via free radical induction, whereas the complexes were formed through physical mixing. The structure and properties of the conjugates and complexes were then characterized. C-H bonds and hydrogen bonds were found to play a crucial role in the formation of the conjugates. Conjugating DOP and EGCG decreased the crystallinity of the polysaccharide and altered the crystal morphology of the polyphenol. The thermal stability of DOP in the EGCG complexes (99.2 °C) was lower than that of free DOP (103.0 °C), whereas that of the DOP in the EGCG conjugates was higher (113.7 °C). The conjugation of EGCG led to an increase in its phenolic content (112.9 ± 1.8 g/kg), which enhanced its antioxidant properties. Indeed, the conjugates had higher antioxidant properties, as seen by an increase in free radical scavenging, reactive oxygen species quenching, and reducing ability, than the complexes. The present study demonstrated that the formation of polysaccharide-polyphenol conjugates could enhance the antioxidant properties of DOP, thereby expanding its range of application as a functional ingredient in foods and beverages.
研究领域:
食品科学
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植物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL PHYSICS
·
2024
·Vol. 160
·期 23
·
IF 3.1
Li Meiyue,Tian Xiao,Du Weiping,Wang Xiujuan,Lei Juncheng,Gao Tianyue,Zou Siyu,Xu Xuefang,Wang Hao,Chen Junhua,Gou Qian
The rotational spectrum of an acrolein–formaldehyde complex has been characterized using pulsed jet Fourier transform microwave spectroscopy complemented with quantum chemical calculations. One isomer has been observed in pulsed jets, which is stabilized by a dominant O=C⋯O tetrel bond (n → π* interaction) and a secondary C–H⋯O hydrogen bond. Splittings arising from the internal rotation of formaldehyde around its C 2v axis were also observed, from which its V 2 barrier was evaluated. It seems that when V 2 equals or exceeds 4.61 kJ mol −1 , no splitting of the spectral lines of the rotational spectrum was observed. The nature of the non-covalent interactions of the target complex is elucidated through natural bond orbital analysis. These findings contribute to a deeper understanding on the non-covalent interactions within the dimeric complex formed by two aldehydes.
研究领域:
物理化学
·
量子化学
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分子光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER COMPOSITES
·
2024
·
IF 4.8
Fangshan Li,Heng Wang,Yuchao Ke,Yuan Hu,Ru Xia
[AMIm]Cl@GNP/SBR composites were prepared by blending ionic liquid 1-allyl-3-methylimidazolium chloride salt([AMIm]Cl) modified graphene nanoplatelets (GNP) and styrene-butadiene rubber (SBR). The mechanical and thermal properties as well as the structure–activity relationship of the obtained composites based on the modified filler were investigated under the synergistic effect of non-covalent and covalent bonding. The experimental results indicated that the modification of GNP by [AMIm]Cl occurred under mild conditions. The dispersibility of [AMIm]Cl@GNP in SBR was enhanced by its participation in vulcanization and the synergistic effect of non-covalent and covalent cross-linking networks such as cation-π, π-π, and hydrogen bonds. This improvement led to an increase in the interfacial bond strength of the composites and a reduction in the interfacial thermal resistance. These effects resulted in both reinforcement and thermal conductivity properties. The tensile strength and elongation at break of [AMIm]Cl@GNP/SBR composites increased from 18.2 to 22.9 MPa and from 737.9% to 923.5%, respectively, when the modification ratio of [AMIm]Cl was 1% and the loading of [AMIm]Cl@GNP was only 4 phr (mass ratio). At the same time, the thermal conductivity in the vertical direction of the composites increased from 0.25 to 0.44 W/(m·K), and the thermal conductivity in the horizontal direction increased from 0.27 to 0.81 W/(m·K). Highlights Modified powder [AMIm]Cl@graphene nanoplatelets were prepared; [AMIm]Cl@graphene nanoplatelet/styrene-butadiene rubber blend was prepared; Mechanical properties of blends significantly improved over blank; The horizontal thermal conductivity of the blends increases obviously; The vertical thermal conductivity of the blends was improved.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）