MICROCHIMICA ACTA
·
2013
·Vol. 180
·期 5
·477-483
·
IF 3.434
Zhu Xiaohua,Jiao Qifang,Zhang Chuyi,Zuo Xiaoxi,Xiao Xin,Liang Yong,Nan Junmin
We have developed a stable and sensitive nonenzymatic glucose sensor by modifying a glassy carbon electrode (GCE) with a composite incorporating nickel(II) oxides and reduced graphene. The oxides were generated by directly electrodepositing nickel on the GCE with a graphene modifier using a multi-potential pulse process, and then oxidizing nickel to nickel(II) oxides by potential cycling. In comparison to the conventional nickel(II) oxides-modified GCE, this new nickel(II) oxides-graphene modified GCE (NiO-GR/GCE) has an about 1.5 times larger current response toward the nonenzymatic oxidation of glucose in alkaline media. The response to glucose is linear in the 20 μM to 4.5 mM concentration range. The limit of detection is 5 μM (at a S/N of 3), and the response time is very short (<3 s). Other beneficial features include selectivity, reproducibility and stability. A comparison was performed on the determination of glucose in commercial red wines by high-performance liquid chromatography (HPLC) and revealed the promising aspects of this sensor with respect to the determination of glucose in real samples.
A stable and sensitive nonenzymatic glucose sensor is developed by preparing the nickel(II) oxides-reduced graphene nanocomposite modified glassy carbon electrode (NiO-GR/GCE), and then used to detect the glucose contents in the commercial red wines. This NiO-GR/GCE also has a high selectivity
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 11
·1686-1696
·
IF 2.7
Xinyue Zhang,Si Wu,Tao Feng,Yuanhao Yan,Shijing Wu,Yinyu Chen,Yu Wang,Qingmiao Wang,Ning Hu,Li Wang
Based on the versatile properties of bio-derived materials, non-enzymatic assays in combination with electronic devices have attracted increasing interest. Here, we report a novel enzyme-free visualization approach for the detection of erythritol, which is a zero-calorie natural sweetener and serves as an ideal sucrose substitute for diabetics or overweight people who need sugar control. The recognition element of the electrochemical biosensor was constructed by catechol modification on a chitosan-based hydrogel film. The signal transduction was achieved by the competitive binding assay of sweeteners. The results show that 2-fluorophenylboronic acid (FPBA) can form a cyclic boronate ester with the ortho-hydroxyls of both reduced catechol and oxidized quinone, impeding the electron transfer and leading to redox signal attenuation. The addition of sweeteners caused a competitive reaction resulting in bonding between the 1,2-diols and FPBA moieties, and in the recovery of the redox signals. Importantly, the pattern of redox signal changes of catechol can be detected optically, as the oxidized quinone state is darker in color than the reduced catechol state. Using a simple cell phone imaging application, we demonstrate that erythritol can be distinguished from other sweeteners in real samples using the oxidized catechol-Chit0/agarose hydrogel film. Thus, we envision that this method could allow diabetics and people who need to control their sugar intake to detect whether the product contains only erythritol in the field or at home. In addition, this work further illustrates the potential of bio-derived materials for performing redox-based functions and enzyme-free visualization assays.
研究领域:
生物化学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-7
·
IF 5.479
Qiao Juan,Liu Qianrong,Wu Han,Cai Huiwu,Qi Li
A fluorescent probe is described for the determination of serum glucose after hepatotoxin-induced liver injury. The probe is based on the use of a water-soluble polymer and has been prepared from a multi-functional azlactone polymer as the linker, amino boronic acid, and Alizarin Red as the signalling moiety. The excitation/emission peaks of the polymeric fluorescent probe are at 468/567 nm. Fluorescence is reduced on addition of glucose. Intensity drops linearly in the 0.1 mM to 14 mM glucose concentration range. The probe was applied to non-enzymatic detection of glucose in rat serum after CCl 4 -induced liver damage.
A polymer based fluorescent probe has been constructed and applied for non-enzymatic monitoring of serum glucose following hepatotoxin induced liver injury.
研究领域:
生物化学
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医学诊断
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材料科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Liqing Du,Zhongping Li,Jiaoli Yao,Guangming Wen,Chuan Dong,Hung-Wing Li
Silicon quantum dots have become one of the most popular nanomaterials in biological applications for their excellent biocompatibility and optical properties . Herein, we synthesized amino-functionalized silicon quantum dots (NH 2 @SiQDs) via a simple microemulsion method, in which silicon tetrachloride and allylamine were used as source of silicon and functional group. NH 2 @SiQDs exhibits good water-solubility, high fluorescence quantum yield and optical stability. A non-enzymatic biosensor of glucose was developed based on the fluorescence quenching of NH 2 @SiQDs in response to glucose. The fluorescence response was linearly proportional to glucose in the concentration range of 1.0 × 10 −6 –9.0 × 10 −5 mol/L and the detection limit was determined to be 3.0 × 10 −7 mol/L. The developed glucose sensor was successfully applied in blood glucose analysis of human serum. Satisfactory result that agreed very well with traditional method was obtained.
研究领域:
生物化学
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纳米生物学
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分析化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
·
IF 3.232
Qiujun Lu,Ting Huang,Jieqiong Zhou,Yue Zeng,Cuiyan Wu,Meiling Liu,Haitao Li,Youyu Zhang,Shouzhuo Yao
Rational design of detection strategy is the key to high-performance fluorescence analysis. In this article, we found that the glucose-induced limitations can greatly enhance the fluorescence of functionalized carbon nanoparticles (CNPs), which are synthesized through one-step thermal pyrolysis method using phenylboronic acid derivative as the precursors. The glucose can assembly onto the surface of the CNPs to form a “shell”, limiting the surfaces' intramolecular rotation and reducing non-radiative decay, which hence resulted in enhanced fluorescence of the CNPs. Under optimal conditions, the fluorescence intensity of the CNPs is nearly 70-fold enhanced, and the method has low detection limit (10 μM) and linear response in the concentration range from 50 μM to 2000 μM. Based on this interesting “target-triggered limitation-induced fluorescence enhancement” phenomenon, a simple and effective non-enzymatic fluorescence enhancement method was developed and successfully applied to the determination of glucose in spiked serum samples. This work provides new insight into the design of fluorescence-enhanced detection strategies based on the limitation-induced property.
研究领域:
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Fuyue Wang,Yanlong Feng,Shi He,Luyu Wang,Manli Guo,Yujuan Cao,Yumin Wang,Ying Yu
Here, we prepared a nickel nanoparticles-loaded three-dimensional porous magnetic graphene-like nanocomposite (NiNPs/3D-PMG) using D113 type macroporous weakly acidic cation exchange resin as the precursor by a simple one-step strategy, which was utilized as a novel electrode modifier for the non-enzymatic electrochemical sensing of glucose. The prepared NiNPs/3D-PMG material was characterized by a series of techniques. In alkaline medium, NiNPs in the nanocomposite modified on electrode surface can be electrochemically transformed to NiOOH, which exhibits high catalytic activity toward glucose oxidation. Benefiting from the large specific surface area of the highly conductive 3D porous graphene-like structure and the catalytic performance of NiOOH, the prepared NiNPs/3D-PMG nanocomposite exhibits prominent electrocatalytic activity toward glucose oxidation. By using a magnetic electrode as the substrate for the immobilization of our NiNPs/3D-PMG with magnetic property, the stability and reusability of the fabricated electrochemical sensor are greatly improved. Under the optimized conditions, a linear amperometric response range from 2 μM to 0.22 mM was obtained, and the detection limit (S/N = 3) was 1 μM. This electrochemical sensor based on the NiNPs/3D-PMG exhibits a rapid response to glucose with high sensitivity, excellent reproducibility and strong anti-interference performance. Furthermore, our sensor has been successfully utilized to detect glucose in human serum with satisfactory results. It provides an effective new method for direct detection of glucose.
研究领域:
分析化学
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生物传感器
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纳米材料
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电化学
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临床检验
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Hao Li,Qi Zhao,Yu-Qi Ge,Zhencheng Chen,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
Cholesterol (CHO) plays a vital role in fundamental cellular life activities, and high CHO levels in the human body can lead to atherosclerosis, making the development of CHO biosensors essential. We prepared a molecularly imprinted electrochemical sensor for non-enzymatic detection of cholesterol by depositing multi-walled carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) for signal amplification on a glassy carbon electrode (GCE) and then modified with a phenol molecularly imprinted membrane (MIM) to develop a CHO enzyme-free detection platform (MIM/rGO/MWCNTs/GCE). The formation of the materials and MIM was characterized using techniques such as scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The sensor exhibited good performance in the linear range of 3.34–100.00 μM using differential pulse voltammetry (DPV), with a limit of detection (LOD) of 1.11 μM and a high sensitivity of 1.613 μA·μM −1 ·cm −2 . The sensor exhibits good reproducibility, stability, repeatability, and selectivity. The developed sensors were applied to detect human serum samples, yielding recoveries in the range of 99.4–100.4 % with a relative standard deviation (RSD) lower than 4.17 %, demonstrating the potential of the developed CHO electrochemical sensors for clinical detection.
研究领域:
分析化学
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生物传感器
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纳米材料
·
电化学
·
生物医学工程
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DOI
高影响因子
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