Foods
·
2025
·Vol. 14
·期 2
·238
·
IF 5.1
Qiuqiu Zhang,Renshuai Huang,Guangjing Chen,Fen Guo,Yan Hu
Suitable planting systems are critical for the physicochemical and bioactivities of strawberry (Fragaria × ananassaDuch.) polysaccharides (SPs). In this study, SPs were prepared through hot water extraction, and the differences in physicochemical characteristics and bioactivities between SPs derived from elevated matrix soilless planting strawberries (EP-SP) and those from and conventional soil planting strawberries (GP-SP) were investigated. A higher extraction yield was observed for EP-SP (5.88%) than for GP-SP (4.67%), and slightly higher values were measured for the average molecular weight (632.10 kDa vs. 611.88 kDa) and total sugar content (39.38% vs. 34.92%) in EP-SP. In contrast, a higher protein content (2.12% vs. 1.65%) and a more ordered molecular arrangement were exhibited by GP-SP. Monosaccharide composition analysis revealed that EP-SP contained higher levels of rhamnose (12.33%) and glucose (49.29%), whereas GP-SP was richer in galactose (11.06%) and galacturonic acid (19.12%). Thermal analysis indicated only minor differences in decomposition temperatures (approximately 225–226 °C) and thermal stability between the samples. However, GP-SP showed a higher enthalpy change (ΔHg= 18.74 J/g) compared to EP-SP (13.93 J/g). Biological activity assays revealed that GP-SP generally exerted stronger non-enzymatic glycation inhibition at both early and final stages (IC50: 7.47 mg/mL vs. 7.82 mg/mL and 11.18 mg/mL vs. 11.87 mg/mL, respectively), whereas EP-SP was more effective against intermediate α-dicarbonyl compounds (maximum inhibition of 75.32%). Additionally, GP-SP exerted superior α-glucosidase inhibition (IC50= 2.4583 mg/mL), in line with kinetic and fluorescence quenching analyses showing a higher enzyme–substrate complex binding affinity (Kis= 1.6682 mg/mL;Ka= 5.1352 × 105M−1). Rheological measurements demonstrated that EP-SP solutions exhibited a pronounced increase in apparent viscosity at higher concentrations (reaching 3477.30 mPa·s at 0.1 s−1and 70 mg/mL) and a stronger shear-thinning behavior, while GP-SP showed a comparatively lower viscosity and lower network order. These findings suggest that different planting systems significantly affect both the molecular structures and functionalities of SPs, with GP-SP demonstrating enhanced hypoglycemic and anti-glycation properties. It is therefore recommended that suitable planting systems be selected to optimize the functionality of plant-derived polysaccharides for potential applications in the food and pharmaceutical industries.
研究领域:
食品科学
·
植物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Guang-Hua Tong,Fei-Yu Zhang,Hui-Xin Zheng,Chao-Ying Xu,Wei Nie,Sanad Abdalbagemohammedabdalsadeg,Ali Akbar Moosavi-Movahedi,Jun Hong,Bao-Lin Xiao
Diabetes is a chronic disease, and long term hyperglycemia and oxidative stress in diabetes patients will lead to non-enzymatic glycosylation of proteins and eventually produce advanced glycation end products (AGEs). The non-enzymatic glycosylation of proteins and the formation and accumulation of AGEs in blood vessels and tissues are closely related to the complications of diabetes. Here, the anti-glycation mechanisms of four natural flavonoids, baicalein (BA), baicalin (BE), catechin (CT), and proanthocyanidinB2 (PB2), are studied using a bovine serum albumin (BSA) glycosylation model. The results show that fructose may induce non-enzymatic glycosylation of BSA with a significant increase in glycation stage products, increase aggregation and decrease hydrophobicity. In the presence of reactive oxygen species H 2 O 2 , it exacerbates non-enzymatic glycosylation of BSA and causes more severe damage to its function and structure. BA, BE, CT, and PB2 may inhibit non-enzymatic glycosylation of BSA, exert anti-glycosylation stage products, anti-aggregation, inhibit hydrophobicity reduction, and protect BSA structure. BA/BE/CT/PB2 forms a complex with BSA to statically quench the endogenous fluorescence of BSA. Molecular docking and molecular dynamics simulation are used to further confirm that the main binding forces between BSA and flavonoids are hydrophobic, but there are also hydrogen bonding and electrostatic interactions.
研究领域:
生物化学
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药理学
·
分子生物学
·
药物化学
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生物物理学
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产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yapu Zhang,Chuanying Zhang,Mengfan Wang,Kexin Du,Xin Peng
The inside of the cell is closely filled with diverse biological macromolecules , which will affect various physiological activities in vivo . It is known that natural products have beneficial effects against the formation of advanced glycation end products (AGEs), but their inhibitory actions in macromolecular crowding environment are still poorly understood. Therefore, to investigate the influence of macromolecular crowding environment, the inhibitory effect of cichoric acid (CA) on the non-enzymatic glycation of bovine serum albumin (BSA) in the absence and presence of crowding reagents (PEG 2000 and PEG 4000) was studied. The results demonstrated that CA could significantly inhibit the formation of AGEs than aminoguanidine in the BSA-fructose model with or without PEG, and the inhibition effect in PBS solution was better than that in macromolecular crowding environment. Moreover, CA presented strong antioxidant capacity and trapping ability of glyoxal, and could stabilize the native structure of BSA caused by the glycation modification, which might be the primary mechanisms for its anti -glycation effect. Additionally, CA tended to bind to site I of BSA mainly through hydrogen bonds and hydrophobic interactions. These findings indicated that CA could be regarded as a high potential anti -glycation inhibitor to prevent the development of diabetic complications.
研究领域:
生物化学
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药理学
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分子生物学
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糖尿病研究
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产品关联
DOI
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 27
·13126-13138
·
IF 6.97
Yurui Zhou,Lu Li,Shenghui Li,Shufei Li,Miao Zhao,Qinghong Zhou,Xiaoqun Gong,Jin Yang,Jin Chang
Diabetic cataracts (DCs) are one of the most common ocular complications of diabetes, and easily causes blindness among diabetics. However, there are limited drugs to delay and prevent DCs. Research studies indicate that oxidative damage of the crystalline lens and nonenzymatic glycosylation of the lens protein play a key role in the pathogenesis of DCs. Hence, we developed a kind of autoregenerative redox nanoparticle, which was CeO2 NPs coated with PEG-PLGA (PCNPs). We first found that PCNPs could work not only as an antioxidant to protect lens epithelial cells from oxidative stress based on the repetitive elimination of reactive oxygen species (ROS), but also as a glycation inhibitor effectively restraining α-crystallin glycation and crosslinking, thereby keeping the lens transparent and alleviating DCs. Experimental results successfully validated the fact that the PCNPs were able to operate in eyes for a long time to attenuate lens opacity. We expect that this strategy will provide promising potential for the treatment of DCs.
研究领域:
药理学
·
眼科学
·
纳米技术
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糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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