ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jianhua Li,Lin Lu,Ping Lin,Feina Ye,Lihong Chen
MicroRNAs (miRNAs) are small molecules with significant regulatory functions that have served as biomarkers for the early diagnosis of disease. Accordingly, it is purposeful to develop a rapid, targeted assay for the sensitive, selective, and determination of target miRNAs. Single-band red emission of upconversion nanoparticles (UCNPs) offer high photostability, large anti-Stokes shifts, and low background. Additionally, improving the accuracy via dual-signal fluorescence is also effective for reducing false positive signals induced by background. Herein, taking advantage of the single-band red emission of UCNPs and DNA complementary nanotechnologies, a target-triggered self-assembled dual-signal response biosensor via a strand displacement cascade is reported. The reported approach was used for the determination of miRNA-222 (a model analyte) in serum and the results were similar to those obtained by the polymerase chain reaction (PCR). The spiked recoveries were from 92.5 to 108.4%, indicating that this biosensor has potential for accurate and sensitive determination of miRNAs in biological samples.
研究领域:
生物化学
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分子诊断学
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纳米生物技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Kai Shi,Baoting Dou,Jianmei Yang,Ruo Yuan,Yun Xiang
Specific and sensitive detection of protein biomarkers is of great importance in biomedical and bioanalytical applications. In this work, a dual amplified signal enhancement approach based on the integration of catalytic hairpin assembly (CHA) and terminal deoxynucleotidyl transferase (TdT)-mediated in situ DNA polymerization has been developed for highly sensitive and label-free electrochemical detection of thrombin in human serums. The presence of the target thrombin leads to the unfolding and capture of a significant number of hairpin signal probes with free 3′-OH termini on the sensor electrode . Subsequently, TdT can catalyze the elongation of the signal probes and formation of many G-quadruplex sequence replicates with the presence of dGTP and dATP at a molar ratio of 6:4. These G-quadruplex sequences bind hemin and generate drastically amplified current response for sensitive detection of thrombin in a completely label-free fashion. The sensor shows a linear range of 0.5 pM–10.0 nM and a detection limit of 0.12 pM for thrombin. Moreover, the developed sensor can selectively discriminate the target thrombin against other non-target proteins and can be employed to monitor thrombin in human serum samples.
研究领域:
生物医学
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生物分析学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Jian Zhu,Bin Yang,Li Peng,Jinwei Wu,Huimin Hao,Shuyan Lou
Perfluorinated compounds (PFCs) are useful man-made chemicals and serve as new emerging organic pollutants due to their environmental and health concerns. Chromatography-mass detection methods often need complex procedure and are also too expensive, so there is a critical demand to develop rapid, inexpensive, easy-to-operate and sensitive methods for PFCs detection. In this work, double fluorescent biosensors (‘DT sensor’ and ‘FT sensor’) have been designed to quantitatively detect long-chain perfluorinated compounds (PFCs), due to their strong hydrophobic interaction with DNA probe or lysozyme fiber. The ratio and rapid fluorescence responses offered more obvious signal changes, and high sensitivity with a limit of detection (LOD) of 0.16 μM (98.2 ppb) for perfluorododecanoic acid (PFDoA). For three PFCs with longer perfluoroalkyl chain (CF 2 ), increased detection sensitivity was achieved due to a stronger hydrophobicity. The fluorescent biosensors showed a good selectivity for long-chain PFCs and served as cross-reactive sensors to differentiate three different long-chain PFCs. The biosensors also had robust signal response in tap water or serum samples, and the LOD can be further lowered to pM (ppt) level after sample preconcentration.
研究领域:
环境科学
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分析化学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
Small
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2020
·Vol. 16
·期 19
·2000460
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IF 11.459
Manshu Peng,Feifei Sun,Na Na,Jin Ouyang
Nanogap antennas are plasmonic nanostructures with a strong electromagnetic field generated at the gap region of two neighboring particles owing to the coupling of the collective surface plasmon resonance. They have great potential for improving the optical properties of fluorophores. Herein, nanogap antennas are constructed using an aqueous solution-based method to overcome the defects of weak fluorescence and photobleaching associated with traditional organic dyes, and a highly sensitive nanogap antenna-based sensing strategy is presented for the detection of low-abundance nucleic acid biomarkers via a target-triggered strand displacement amplification (SDA) reaction between two DNA hairpins that are tagged to the tips of gold nanorods (Au NRs). In the presence of targets, end-to-end Au NR dimers gradually form, and the fluorophores quenched by the Au NRs exhibit a dramatic fluorescence enhancement due to the plasmon-enhanced fluorescence effect of nanogap antennas. Meanwhile, the SDA reaction results in secondary amplification of fluorescence signals. Combined with single-molecule counting, this method applied in miRNA-21 detection can achieve a low detection limit of 97.2 × 10 −18 m . Moreover, accurate discrimination between different cells through miRNA-21 imaging demonstrates the potential of this method in monitoring the expression level of low-abundance nucleic acid biomarkers.
研究领域:
纳米技术
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分子生物学
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生物医学工程
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
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2018
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IF 4.244
Qian Luan,Xin Xiong,Ning Gan,Yuting Cao,Tianhua Li,Dazhen Wu,Youren Dong,Futao Hu
An ultrasensitive colorimetric aptasensor was developed for antibiotics detection, with chloramphenicol (CAP) as model target, using DNAzyme labeled Fe-MIL-88-Pt as novel peroxidase mimic signal tags and target-triggered circular strand-displacement polymerization (CSDP) for signal amplification. The system consists of two components which can partially hybridize with each other: one is capture probe which was formed through immobilizing hairpin DNA containing aptamer sequence on magnetic beads (MB-cDNA), another is signal tag which was constructed through labeling single strand DNAzyme (G-quadruplex/Hemin) which can partially hybrid with cDNA on platinum nanoparticles functionalized Fe-MIL-88 (MIL-88-Pt-DNAzyme). All components of MIL-88, Pt and DNAzyme in the tag can act as peroxidase mimic to triply catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 into a blue-colored oxidized TMB (oxTMB) for the colorimetric readout. Thus distinctive signal can be observed by naked eye even in presence of 0.02 nM tags. In the presence of target and primer, cDNA loop can open to form cDNA/CAP intermediates, enabling primer to hybridize with the exposed sequences of the cDNA, which initiated target assisted CSDP recycles. Then numerous signal tags were released into supernatant to catalyze TMB for color development. There was a liner relationship between the absorbance and the concentration of CAP in the range of 0.1 pM (0.0323 pg/mL) to 1000 pM (323 pg/mL) with the detection limit of 0.03 pM (0.0097 pg/mL). The ultra-high sensitivity was ascribed to the multiplex catalytic activities from the tags and CSDP based signal amplification. Furthermore, this method can produce signals being observed by naked eye to facilitate in-situ detection and be further extended to detect other antibiotics in food just by simply replacing cDNA on the sensing system.
研究领域:
生物化学
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分析化学
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纳米技术
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Lihong Zhou,Yamin Xiong,Hui Wang,Aihong Yin,Xueying Zhang,Huiling Li,Qinghua Fu,Peili Huang
Monitoring copper (Cu) species in bio-samples is critical for the investigation and auxiliary diagnosis of Cu-metabolism disorders. Herein, a target-triggered DNAzyme walker, consisting of Carboxyfluorescein (FAM)-labeled substrates of the Cu-DNAzyme (Cu-Sub) coated gold nanoparticles (AuNPs) ( [email protected] track) and the enzyme chain of the Cu-DNAzyme (Cu-Enzy) functionalized magnetic beads ( [email protected] 3D walking unit), was designed for the sensitive detection of copper species. Upon addition of copper ion (Cu 2+ ), DNAzyme was activated and triggered the walking process, thereby releasing FAM-labeled fragments and recovering fluorescence. When compared with free-running Cu-Enzy or co-conjugated Cu-Enzy on AuNPs walking units, the [email protected] walking unit exhibited a higher cleavage efficiency, shorter detection time (40 min), and a lower limit of detection (LOD = 3 nM). This improved performance was mainly attributed to multivalent interactions between the 3D track and the 3D walking unit which dramatically increased the affinity and cleavage efficiency. Importantly, good accuracy was observed for copper species analysis in clinical serum samples (n = 130), relative to other instrumentation, inductively coupled plasma atomic emission spectrometry (ICP-AES) ( P > 0.01). The serum detection data indicated that total, exchangeable and relative exchangeable Cu variables could serve as potential indicators for the auxiliary diagnosis of Cu-related disorders. Thus, our target-triggered DNAzyme walker provides improved technical support for the in-depth study of clinical Cu metabolic-related diseases.
研究领域:
分析化学
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生物化学
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纳米技术
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临床诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）