MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Tianyao Wang,Wenming Xiong,Xingguo Tian,Xiaoyan Xu
MXene quantum dots (MQDs) exhibit vast application prospection the field of fluorometric sensing, but how to efficiently utilize MQDs for the selective detection of two components still remains a challenge. Herein, an off–on fluorescence sensor based on ethylenediamine-functionalized Ti 3 C 2 MQDs (EDA-MQDs) was designed to selectively and sequentially detect Fe 3+ and histamine. EDA-MQDs were synthesized using a facile ultrasonic-hydrothermal method and exhibited bright blue fluorescence with a quantum yield up to 21.12 %. Inner filter effect occurred between Fe 3+ and EDA-MQDs, resulting in fluorescence quenching at 460 nm. Meanwhile, on account of the strong chelation between imidazole group on histamine and Fe 3+ , the Fe 3+ concentration in the system was reduced, so the fluorescence of EDA-MQDs was recovered. Thus, the as-designed fluorescence probe was used for dual-detection of Fe 3+ and histamine in real samples with satisfactory sensitivity and selectivity. The proposed sensing method was verified to possess good linear response in the range of 1–100 μM and 1–60 μM with detection limits of 0.11 μM and 46 nM for Fe 3+ and histamine, respectively. This work provided a highly effective tool for selective detecting histamine and Fe 3+ as well as tendered a novel strategy for functionalized Ti 3 C 2 MQDs in food analysis.
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
CARBON
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2015
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IF 6.196
Zhan Zhou,Qianming Wang,Jinyong Wang,Cheng Cheng Zhang
New chemical sensors based on lanthanide emission that enable sequential detection of two targets in live cells have been developed. The indicator molecules (unsaturated europium complexes) were covalently immobilized on carbon quantum dots (CQDs), and a water soluble fluorescent nano-probe ( CQDs-DTPA-Eu ) with biocompatibility was fabricated. A novel stepwise assembly method was used to generate unique responses to two different targets sequentially. Rapid and highly selective detection of tetracycline (Tc) in water and milk (detection limit 32 nM) were demonstrated. The in situ generated CQDs-DTPA-EuTc was non-emissive, but, upon addition of hydrogen peroxide (detection limit 21 nM) intense red luminescence was observed. Highly reproducible results were obtained in Plat-E cells, demonstrating the identification of tetracycline and H 2 O 2 in cellular environment with high accuracy.
研究领域:
生物化学
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分析化学
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细胞生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Jie Zhang,Xibin Zhou,Jun Wang,Dawei Fang
In this work, a novel Eu 3+ -DTPA-bis(AMC) complex with red luminescence was designed and synthesized for sequential detection of Cu 2+ and biothiols (Cys/Hcy/GSH) based on the displacement strategy with the good selectivity, high sensitivity, and large Stokes shift (288 nm). The possible detection mechanism was verified by UV–vis, the high-resolution mass spectrometry, and the fluorescence decay curve. The experimental parameters, including the solution pH, the incubation time, the concentration ratio of Eu 3+ -DTPA-bis(AMC) to Cu 2+ and biothiols concentration, were optimized. Under the optimal conditions, it shows a good linear relationship between the concentration (0–10 μM) of Cu 2+ and the fluorescence intensity of Eu 3+ -DTPA-bis(AMC), with a low detection limit of 0.065 μM. The linear range and the limit of detection of the Eu 3+ -DTPA-bis(AMC)/Cu 2+ system for Cys/Hcy/GSH were 2.5–22.5/5–45/5–50 μM and 0.11/0.07/0.05 μM, respectively. Surprisingly, the high or low concentration of Eu 3+ -DTPA-bis(AMC)/Cu 2+ can significantly affect the selectivity of the sensing system to biothiols (Cys/GSH/Hcy). When the concentration of the Eu 3+ -DTPA-bis(AMC)/Cu 2+ system is 10.0 μΜ, it could recognize biothiols (Cys/GSH/Hcy) from other substances, but when the concentration is as low as 3.3 μM, it could further specifically distinguished Cys from Hcy/GSH. Owing to the high anti-interference characteristics, accuracy and specificity, the sensing system was well applied to the cascade detection of Cu 2+ in actual environmental samples and Cys in biological and food samples, including FBS, urine, milk, beverage, fresh juice with the satisfactory recoveries from 96.20 to 106.80 %.
研究领域:
分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2019
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IF 4.916
Can Chen,Fenghua Geng,Yongxiang Wang,Haidong Yu,Lin Li,Sheng Yang,Jinhua Liu,Wei Huang
The present work constructs a sequentially triggered nanoswitch (STN) for sequential detection of Cr 3+ , P 2 O 7 4− (PPi) and alkaline phosphatase (ALP) depending on polythymine (T40) templated fluorescent Cu nanoparticles (Cu NPs). A significant phenomenon is that Cr 3+ can only causing 5% QE of fluorescent Cu NPs synthesized by lower than 500 μM Cu 2+ , but the fluorescence of the Cu NPs synthesized by more than 500 μM Cu 2+ can be quenched up to 90% QE by the same concentration of Cr 3+ . Then the quenched fluorescence of CuNP-Cr 3+ complex provides a sensing platform for PPi due to the strong binding between Cr 3+ and PPi, resulting in dissociation of Cr 3+ from the surface of Cu NPs and the recovery of fluorescence emission . Further ALP hydrolysis of PPi disrupts Cr 3+ -PPi assemble and Cr 3+ is released to interact with Cu NPs, which induces fluorescence quenching again. Thus, sequentially detection of Cr 3+ (LOD, 0.03 μM), PPi (LOD, 0.005 μM) and ALP (LOD, 0.125 mU/mL) was successfully implemented with high sensitivity and selectivity. The sensor is also successfully used for Cr 3+ , PPi and ALP assays in the human serum. Additionally, the sensitive “on-off-on-off” sensing behavior of the Cu NPs allow three chemical inputs (Cr 3+ , PPi and ALP) to construct a logic gate.
研究领域:
生物化学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Guo Wei,Chuanliang Wang,Rui Wang,Xuhui Geng,Jinhua Li,Lingxin Chen,Zhihua Song
In this study, a novel carbon dots-based system was developed for the sequential quantification of Au 3+ and L-cysteine (L-Cys). The system comprises N,F-doped carbon dots (N,F-CDs), a custom-designed miniaturized fluorimeter, and test strips. The N,F-CDs exhibit outstanding optical properties, including a large Stokes shift (127 nm) and high fluorescence intensity. The test strips offer low-cost, low-volume sample consumption, while the miniaturized fluorimeter provides high sensitivity in a compact format. The system demonstrated excellent analytical performance, including high sensitivity (limits of detection: 0.048–0.488 μmol/L for Au 3+ and 8.7–40.9 nmol/L for L-Cys), minimal matrix interference (within ±20 %), high accuracy (97.5 % to 104.5 % for Au 3+ , 97.6 % to 107.5 % for L-Cys), good precision (2.9 % to 4.5 % for Au 3+ , 1.2 % to 7.0 % for L-Cys), robust stability, and strong inter/intra-day reproducibility (relative standard deviations ≤2.1 %). Furthermore, it showed high selectivity for Au 3+ and L-Cys in complex matrices such as tap water, milk, and lemon-flavored beverages.
研究领域:
分析化学
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纳米技术
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环境监测
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生物分析
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材料科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Fanqiang Bu,Bing Zhao,Wei Kan,Liyan Wang,Bo Song,Jianxin Wang,Zhe Zhang,Qigang Deng,Guangming Yin
Ag + and SCN − play extremely important roles in the fields of the physiology and environment. In this work, on the basis of phenanthro[9,10- d ]imidazole derivative ( DIPIP ) which can exhibit the aggregation-induced emission (AIE) properties in aqueous solution, we achieved a sequential on-off-on switch for Ag + and SCN − with high selectivity and sensitivity. A remarkable fluorescence quenching effect of Ag + on the probe DIPIP was observed with 1:2 stoichiometry , Subsequently, the fluorescence intensity of in situ generated DIPIP -Ag + ensemble was easily switched on after the interaction between Ag + and SCN − , which was attributed to the stronger affinity of SCN − to capture Ag + . In particular, the extreme limits of detection (LOD) for Ag + and SCN − in standard solutions were as low as to be 74.5 nM and 7.8 nM, respectively. Furthermore, the probe DIPIP and the DIPIP -Ag + ensemble could be used to detect Ag + in the real water and SCN − in smoker saliva samples, respectively. In addition, the sequential “on-off-on” fluorescence mode of DIPIP to Ag + and SCN − were also successfully applied in living HeLa cells.
研究领域:
分析化学
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环境科学
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生物化学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Yanxia Xiao,Wenjuan Dong,Huiping Wang,Yumin Hao,Zihan Wang,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
A novel visual nanoprobe was developed for the sequential detection of morin and zinc ion (Zn 2+ ) based on Cl and N co-doped carbon quantum dots (ClNCQDs) via a fluorometric and colorimetric dual-readout mode. The yellow fluorescence ClNCQDs was synthesized by the one-step hydrothermal treatment of o -chlorobenzoic acid and p -phenylenediamine. The most distinctive property of the ClNCQDs is the large stokes shift (177 nm), which is significantly higher than other reported CQDs. The fluorescence of the ClNCQDs can be effectively quenched by morin based on the synergistic effect of IFE, electrostatic interaction, and dynamic quenching process, and recovered upon the addition of Zn 2+ due to strong interaction between morin and Zn 2+ . The nanoprobe exhibited favorable selectivity and sensitivity toward morin and Zn 2+ with detection limits of 0.09 µM and 0.17 µM, respectively. Simultaneously, the color of the ClNCQDs solution was changed (light-pink → faint-yellow → dark-yellow) along with the variation of the fluorescence signal of the ClNCQDs. This proposed nanoprobe was successfully applied for morin and Zn 2+ analyses in actual samples and live cells with high accuracy. The results of this study demonstrate the great application prospects of the ClNCQDs for morin and Zn 2+ detection in complex actual samples and biosystems.
研究领域:
纳米技术
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分析化学
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材料科学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）