Coatings
·
2024
·Vol. 14
·期 2
·223
·
IF 2.9
Kai Liu,Wen Lai,Jianyong Wu,Yongjian Lu
The failure of bone defect repair caused by bacterial infection is a significant clinical challenge. However, the currently utilized bone graft materials lack antibacterial properties, necessitating the development of bone repair materials with both osteoinductive and antibacterial capabilities. Graphene oxide (GO) has garnered considerable attention due to its distinctive physical, chemical, and biological characteristics. In this study, we prepared a graphene oxide-poly(lactic acid) (GO-PLA) film with exceptional biological properties. In vitro investigations demonstrated that the GO-PLA film substantially enhanced the adhesion and proliferation capacity of rat bone marrow mesenchymal stem cells (rBMSCs). Furthermore, we observed augmented alkaline phosphatase activity as well as increased expression levels of osteogenic genes in rBMSCs cultured on the GO-PLA film. Additionally, we evaluated the antibacterial activity of our samples using gram-positive Streptococcus mutans (Sm) and gram-negative Actinobacillus actinomycetemcomitans (Aa). Our findings revealed that GO doping significantly inhibited bacterial growth. Moreover, implantation experiments conducted on rat skull defects demonstrated excellent guided bone regeneration performance exhibited by the GO-PLA film. Overall, our results indicate that the GO-PLA film possesses outstanding osteogenic and antibacterial properties, making it a promising biomaterial for bone tissue regeneration.
研究领域:
生物材料学
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骨组织工程
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医学材料
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产品关联
DOI
高影响因子
Applied Materials Today
·
2020
·
IF 8.352
Youguo Liao,Qiulin He,Feifei Zhou,Yi Hong,Xinyu Wu,Haoyu Wu,Xianzhu Zhang,Varitsara Bunpetch,Jingwei Zhang,Guo Ye,Weiliang Shen,Shufang Zhang,Hongwei Ouyang
Large area skull defects accompanied by craniocerebral injury are life-threating diseases that are difficult to treat due to the large area of damage and intracranial hypertension. An autologous bone is an ideal source for the regeneration of bone, including skull defects. However, two-step treatment, including craniectomy and cranioplasty, is the main strategy in clinical practice to stabilize intracranial pressure, but still remains a huge obstacle in both the preservation of auto-bone fragments and protection of exposed brain tissue. In this study, an elastic auto-bone patch was developed to provide a new and one-step method for repairing large skull defects which are currently repaired with two stages of surgery. The elastic auto-bone patch is formed by bonding auto-bone fragments together using hydrogels, which is then re-implanted to bone defects to repair large skull defects. The auto-bone patch could adapt to changes in intracranial pressure to protect brain tissue for damage, integrate well with surrounding bone tissues, and promote bone regeneration. After 8 weeks implantation, the large-size skull defect is filled with new formed bone tissues in the group of treating with auto-bone patch. This study introduced a new procedure on the application of elastic auto-bone patch to repair large area skull defects.
研究领域:
医学
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生物材料学
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神经科学
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Yicai Luo,Ziwei Wu,Yingjuan Zhang,Yang Qiao,Yinge Wei,Xuan Yan,Xiangyu Ma,Xianxian Huang,Xiaoxia Zhong,Zhimao Ye,Xinping Lu,Hongbing Liao*
Introduction: Diabetes mellitus often leads to bone metabolism disorders, hindering bone regeneration and delaying the healing of bone defects. β-Ecdysone, a plant-derived hormone known for its wide range of physiological activities, possesses hypoglycemic effects and promotes osteogenic differentiation. This study developed a composite PLGA slow-release scaffold loaded with β-ecdysone to enhance its bioavailability through topical administration and to investigate its potential to heal diabetic bone defects.Methods: The composite scaffolds were fabricated using solution casting/particle leaching and freeze-drying techniques. Then a series of characterizations were subjected to test the performance of composite scaffolds, and in vitro safety of the composite scaffolds was tested by CCK8 assay and live/dead cell staining. Further, micro-CT and histology to evaluate the effect of β-E/PLGA composite scaffolds on healing of skull defects in diabetic rats at 4 and 8 weeks after implantation. Simultaneously, the safety of the scaffolds in vivo was also evaluated.Results: The material characterization results indicated that, in comparison to the single-pore size scaffold, the composite scaffold exhibited superior porosity, swelling ratio, drug loading capacity, and mechanical properties. Additionally, the composite scaffolds showed appropriate degradation performance and sustained drug release profiles. The CCK8 cytotoxicity assay and live/dead cell staining demonstrated that BMSCs survived and proliferated on the composite scaffold under both low-glucose and high-glucose conditions. Micro-CT and histological investigation demonstrated that β-E/PLGA composite scaffolds promoted new bone growth in the skull defect region of diabetic rats.Conclusion: Overall, these findings suggest that the β-E/PLGA composite scaffolds promote the healing of bone defects in diabetic rats. The combination of β-ecdysone and tissue-engineered scaffolds presents a promising approach for treating diabetes-related bone defects.
研究领域:
生物医学工程
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内分泌学
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组织工程学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Song Lv,Xiaojie Lian,Haonan Feng,Bo Yang,Zehua Liu,Tong Fu,Liqin Zhao,Di Huang
Although 3D printed scaffolds are widely used in irregularly shaped bone defects, additional steps often need to be introduced when fabricating structures with curvature. In contrast, 4D printing has a unique advantage in the fabrication of scaffolding with a curved structure. Bone defects such as skull is generally curved, so a self-bending scaffold would be more appropriate for the cranial defect site. This paper presents a novel self-bending SAGMA hydrogel was loaded with nano-spherical mineralized collagen, then fabricated by a 4D printing method, which achieves adjustable self-bending through three-step crosslinking. When subjected to UV light irradiation, variations in gradient of photo-crosslinking are induced within the scaffold. This gradient of photo-crosslinking serves as the foundation for the scaffold's self-bending. The scaffold exhibited self-bending after crosslinking with calcium ions and chitosan, respectively, with curvature ranging from 0.05 mm −1 to 0.446 mm −1 . In vivo experiments demonstrated the efficacy of the scaffold in enhancing the repair of cranial bone defects and promoting new bone formation in rats, as evidenced by microcomputed tomography and histochemical analysis. Therefore, this self-bending scaffold provides a potentially effective method for the clinical treatment of skull defects with curvature.
研究领域:
生物材料学
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组织工程学
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2024
·
IF 3.9
Tao Ge,Jianxiang Jin,Kuan Feng,Xialong Gu,Gengfan Ye,Taotao Shi,Jia Li,Hao Wang,Hongcai Wang,Maosong Chen
Cranial defect repair remains a significant challenge in neurosurgery, and designing material complexes that can support bone regeneration while minimizing complications such as infection and inflammation could help alleviate this clinical challenge. This study presents a photothermal hydrogel complex with a controlled rapid gelation process, PDA-G-A-H, which integrates photothermal polydopamine nanoparticles (PDA NPs) with gentamycin (G) and alendronate acid (A). Furthermore, the incorporation of the injectable hydrogel Pluronic F127 and collagen (H) made this composite hydrogel (PDA-G-A-H) suitable for the multifaceted needs of cranial defects. The PDA-G-A-H hydrogel exhibited superior biocompatibility, as evidenced by high cell viability and minimal hemolysis, making it a safe candidate for biomedical applications. In vitro assessments with MC3T3-E1 cells demonstrated that this hydrogel enhanced mineralization and osteogenic differentiation, and significant upregulation of key osteogenic markers was subsequently detected. The antibacterial activity of the hydrogel against Staphylococcus aureus and Staphylococcus epidermidis was also investigated. The results of the RT‒PCR analysis revealed the potential for inhibiting biofilm formation. The hydrogel composite combines biocompatibility, osteoinductive, and antibacterial potential. It has translational potential for cranial defect repair and other bone regeneration therapies.
研究领域:
生物材料学
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骨组织工程
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医学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Xiao Fei,Shi Jingjing,Zhang Xinhai,Hu Min,Chen Kangming,Shen Chao,Chen Xiaodong,Guo Yaping,Li Yang
Reducing the incidence of bone defects caused by trauma and other primary diseases is an urgent task in modern society. In the present study, we developed a gadolinium-doped whitlockite/chitosan (Gd-WH/CS) scaffold and assessed its biocompatibility, osteoinductivity, and bone regeneration capacity for the treatment of calvarial defect in a Sprague-Dawley (SD) rat model. The Gd-WH/CS scaffolds possessed a macroporous structure, with a pore size ranging 200–300 μm, which facilitated the growth of bone precursor cells and tissues into scaffold. Results of cytological and histological biosafety experiments showed that both WH/CS and Gd-WH/CS scaffolds were non-cytotoxic to human adipose-derived stromal cells (hADSCs) and bone tissue, which demonstrated the excellent biocompatibility of Gd-WH/CS scaffolds. Results of western blotting and real-time PCR analysis provided a possible mechanism that Gd3+ ions in the Gd-WH/CS scaffolds promoted the osteogenic differentiation of hADSCs through the GSK3β/β-catenin signaling pathway and significantly upregulated the expression of osteogenic related genes (OCN, OSX and COL1A1). Finally, in animal experiments, SD rat cranial defects were effectively treated and repaired with Gd-WH/CS scaffolds due to its appropriate degradation rate and excellent osteogenic activity. This study suggests the potential utility of the Gd-WH/CS composite scaffolds in treating bone defect disease.
研究领域:
生物材料学
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骨组织工程
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Tissue Engineering and Regenerative Medicine
·
2025
·Vol. 2025
·期 1
·9964384
·
IF 2.6
Junteng Yan,Shuhao Qi,Yiwei Zhao,Peng Tian,Ning Kong,Weigang Ma,Peng Yan,Jiewen Zhang,Xu Gao,Huanshuai Guan,Pei Yang,Qin Lian,Kunzheng Wang
Managing large, critical-sized bone defects poses a complex challenge, especially when autografts are impractical due to their size and limited availability. In such situations, the development of synthetic bone implants becomes crucial. These implants can be carefully designed and manufactured as potential bone substitutes, offering controlled parameters such as porosity, hardness, and osteogenic cues. In this study, we employed digital light processing (DLP) technology to construct an alumina ceramic scaffold featuring a triply periodic minimal surface (TPMS) structure for bone transplantation. The scaffold was filled with type I collagen to enhance cell infiltration [1], thereby increasing the total surface area. In addition, type I collagen is a carrier for both bone morphogenetic protein-2 (BMP-2) and zoledronic acid (ZA). Using a clinically relevant rabbit cranium defect model, the scaffold underwent in vivo assessment for its functionality in repairing critical-sized bone defect (approximately 8 mm). Four groups of animal experiments were carried out including the control group, the gyroid scaffold group, the type I collagen-loaded scaffold group, and the bioactive factor-functionalized scaffold group. Our animal-based study results revealed that the gyroid scaffold, functionalized with bioactive molecules, provided a conductive surface for promoting increased bone formation and enhancing the healing process in critical-sized long bone and cranium defects. These findings offer preclinical evidence, supporting the use of a TPMS structure composite scaffold and present compelling support for its application as an advanced synthetic bone substitute in the future.
研究领域:
生物医学工程
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再生医学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 13
·2109882
·
IF 18.808
Jie Zhang,Boyou Zhang,Zefeng Zheng,Qingyun Cai,Jingcheng Wang,Qiang Shu,Lijia Wang
Tissue-engineered bones have therapeutic potential for critical-size bone defects; however, the production of high quantities of the tissue-engineered bones with osteo-induction ability remains a huge challenge. Hyperthermia has been shown to up-regulate the expression of osteogenesis-related proteins to efficiently to promote bone regeneration. In this study, the authors develop a novel photothermal tissueengineered bone (PTEB) with osteo-induction ability and a biomimetic cellular environment. PTEB is generated by seeding rat bone mesenchymal stem cells (rBMMSCs) in the photothermal MoS 2 -biotin-garose-gelatin scaffold, and then overlaying the scaffold using osteo-induction extracellular matrix (OiECM). The rBMMSCs act as seeding cells, while OiECM provide a biomimetic microenvironment for repairing critical-sized cranial defects in rats. The results show that the PTEB exhibit high biocompatibility and osteo-induction ability under near-infrared (NIR) radiation. Results of in vitro experiments show that PTEB under NIR radiation promote proliferation and osteogenic differentiation of rBMMSCs. Furthermore, the PTEB implantation under NIR radiation significantly induces regeneration of bone in critical-size bone defects in rats 12 weeks after implantation. These findings indicate that PTEB has great potential in regenerative medicine and may represent an effective replacement for autografts used commonly in bone tissue engineering.
研究领域:
生物医学工程
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再生医学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2020
·
IF 6.092
Peiming Liu,Lian Sun,Zeyi Wang,Jin Sun,Yaning Dong,Lin Cao,Jian Shen,Wei-Bing Zhang,Pingsheng Liu
Designing smart scaffolds to reduce administration dosage under the premise of functional healing of bone defects to avoid the severe side effects associated with BMP-2 treatments is one of the essential goals in bone tissue engineering. Here, we report a novel biodegradable PLGA/PSBMA composite as the scaffold for bone tissue engineering. The introduction of zwitterionic PSBMA components can alter the intrinsic burst degradation behavior of PLGA and enable a sustained degradation of the scaffold over the time. The PLGA/PSBMA scaffold can sequester rhBMP-2 and enable a sustained release of the sequestered rhBMP-2 with preserved bioactivity. Furthermore, PLGA/PSBMA scaffolds were able to guide robust healing of critical-sized nonunion calvarial defects (5 mm) at an ultralow dose of 400 ng/scaffold, at which level successful healing of critical-sized bone defects has never been reported. These findings indicate the PLGA/PSBMA scaffolds as novel high-efficiency rhBMP-2 delivery vehicles for bone tissue engineering, and the concept of utilizing the material, which is capable of maintaining the bioactivity of the proteins in the preparation of scaffolds, may open a new avenue for the design of smart scaffolds/vehicles for high-efficiency protein/bioactive drug therapies.
研究领域:
材料科学
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生物医学工程
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Zongpu Han,Yixiong Lin,Xinyu Guo,Jieyun Xu,Xiaomeng Gao,Ruihan Yang,Yuan Zhao,Mixiao Gui,Linjun Zhang,Yuanlong Guo,Zetao Chen
The strategy of re-organogenesis provides an optimal framework for restoring complex organ structures and functions in adult damage. While the focus has often been on restoring organogenesis stem cells, there is limited investigations of reverting the environmental niche to support this approach. The guiding principle of “Nature selects the fittest to survive” drives the intricate dynamic changes in cellular events within the niche environment, especially through immune surveillance. The extracellular matrix (ECM) serves as the “self-associated molecular patterns” of the niche, containing extensive data on cell-niche reaction data and acting as the active tuner of immune surveillance. In this study, hyaluronic acid (HA) is identified as a unique component of the ECM in cranial osteo-organogenesis. Mechanistically, HA activates the Cluster of Differentiation 44 (CD44)-Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK)-Ets Variant 1/5 (ETV1/5)- Mannose Receptor C-Type 1 (MRC1) axis in macrophages, establishing a distinct immune surveillance during osteo-organogenesis. Furthermore, HA is utilized as a novel engineered material for an “Osteo-organogenesis niche”, restoring immune surveillance and synergistically regulating stem cells to achieve re-osteo-organogenesis in cranial defects of rats. Taken together, the study unveils a previously unknown strategy for leveraging re-organogenesis by utilizing “organogenesis niche” ECM engineered materials to manipulate immune surveillance, thereby comprehensively regulating stem cells and other tissue cells effectively for re-organogenesis.
研究领域:
再生医学
·
免疫学
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组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2022
·Vol. 17
·期 5
·055007
·
IF 4.103
Lin Wu,Yunchao Wu,Liu Jingjin,Suwan Liu,Qianzhe Li,Mengyu Yang,Miaomiao Zhang,Qing Ji,Feimin Zhang,Fengyi Du
Calvarial bone defect remains a clinical challenge due to the lack of efficient osteo-inductive agent. Herein, a novel calcium and phosphorus codoped carbon dot (Ca/P-CD) for bone regeneration was synthesized using phosphoethanolamine and calcium gluconate as precursors. The resultant Ca/P-CDs exhibited ultra-small size, stable excitation dependent emission spectra and favorable dispersibility in water. Moreover, Ca/P-CDs with good biocompatibility rapidly entered the cytoplasm through endocytosis and increased the expression of bone differentiation genes. After mixing with temperature-sensitive hydrogel, Ca/P-CDs were injected in situ into calvarial defect and promoted the repair of bone injury. These Ca/P-CDs provide a new treatment method for the bone repair and should be expended the application in the biomedical fields.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Li Hui,He Zihao,Li Wenjing,Li Jiao Jiao,Lin Jianhao,Xing Dan
Bone regeneration strategies based on mesenchymal stem cell (MSC) therapy have received widespread attention. Although MSC incorporation into bone scaffolds can help with the repair process, a large number of studies demonstrate variable effects of MSCs with some noting that the inclusion of MSCs does not provide better outcomes compared to unseeded scaffolds. This may in part be related to low cell survival following implantation and/or limited ability to continue with osteogenic differentiation for pre-differentiated cells. In this study, we incorporated MSCs into gelatin microcryogels to form microtissues, and subjected these microtissues to osteogenic induction. We then mixed as-formed microtissues with those subjected to 6 days of osteogenic induction in different ratios, and investigated their ability to induce in vitro and in vivo osteogenesis during self-assembly. Using a full-thickness rat calvarial defect model, we found that undifferentiated and osteogenically induced microtissues mixed in a ratio of 2:1 produced the best outcomes of bone regeneration. This provides a new, customizable cell-based therapeutic strategy for in vivo repair of bone defects.
研究领域:
组织工程
·
干细胞生物学
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再生医学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）