SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Wenjun Zhou,Qiang Gao,Darui Liu,Chen Li,Shu Liu,Kaisheng Xia,Bo Han,Chenggang Zhou
A novel salicylaldehyde bis-Schiff-base probe decorated with imidazolium ionic liquid moieties at both ends (SAS-IMIs) was designed and facilely synthesized as a colorimetric/ratiometric sensor to visually detect Cu 2+ and Pd 2+ in pure aqueous media. Due to the positively charged characters of its head and tail, the SAS-IMIs exhibited high water solubility with a potential advantage in minimizing the self-aggregation. More importantly, simply by varying the solution pH, colorimetric/ratiometric sensing detection of individual metal ion (Cu 2+ or Pd 2+ ) was realized without any mutual interference. Subsequently, sensitive, selective, and differential detections for Cu 2+ (LOD: 0.080 μM) and Pd 2+ (LOD: 0.076 μM) in 100% aqueous solutions were achieved, which proved to be applicable for real water samples. Results from density functional theory (DFT) calculations unveiled the Cu 2+ /Pd 2+ -binding properties of SAS-IMIs, which were in accordance with the experimental observations. Furthermore, a SAS-IMIs-based solid phase sensor was fabricated, which manifested satisfactory detection abilities for Cu 2+ and Pd 2+ .
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 146
·期 3
·1032-1039
·
IF 3.978
Jianlin Pu,Chang Liu,Bin Wang,Pei Liu,Yanzi Jin,Jiucun Chen
Carbon dots (CDs) have demonstrated considerable potential for use in sensing and bioimaging applications based on their unique intrinsic properties. However, CDs have typically been used as fluorescence sensors, as opposed to indicators, based on their ultraviolet absorption and discoloration performance. In this study, orange red-emitting CDs with a high quantum yield (QY) of 53% were constructed from 2,3-diaminopyridine via solvothermal synthesis. Different from the fluorescence quenching behavior reported previously, the constructed CDs demonstrated a unique character in their ultraviolet absorption and fluorescence emission in the presence of Fe3+. The color of the CD solution varied from mauve to orange following the addition of Fe3+ at concentrations exceeding 1 μM, which enabled these CDs to be used in the determination of the presence of Fe3+ in lake water. In addition, due to their negligible cytotoxicity, good solubility, and adequate dispersity, an outstanding cellular probe with near-infrared fluorescence was established. Overall, this study presents a unique CD-based sensor, details its fluorescence mechanism, visual colorimetry, and ultraviolet absorption variations, and confirms its applicability in near-infrared cellular imaging and environmental analyses.
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Aiping Deng,Xuan Li,Xiaoxing Fan,Yajie Li,Yelin Han,Tianzhu Guan,Shuo Wang,Guoqiang Zhu,Zhenquan Yang,Wenyuan Zhou
Bacteriophage-based biosensors have the potential for rapid, convenient, and sensitive detection of Staphylococcus aureus in food products; however, their effectiveness is often limited by the host range of the bacteriophage. This study presents a new colorimetric biosensor using the S. aureus phage SapYZU04 exhibiting a broad host range, and a MnFeO nanozyme for the effective detection of S. aureus in food products. Notably, SapYZU04 exhibited excellent features, such as a high titre (1.0 × 10 10 PFU/mL), a short latency period (5 min), a large burst size (210 PFU/cell), a wide host spectrum (98.95%, 94/95), and good stability at pH 3.0–12.0. The biosensor, referred to as SapYZU04@MnFeO was constructed by immobilising SapYZU04 on the MnFeO nanozyme via electrostatic interactions. SapYZU04@MnFeO demonstrated a broad detection range of bacterial concentrations, particularly within the range of 3 × 10 2 to 3 × 10 9 CFU/mL, with a low detection limit of 65 CFU/mL and rapid detection time (14 min). Furthermore, it exhibited high accuracy in detecting and quantifying live S. aureus with recovery rates ranging from 92.21% to 105.56%. The chromogenic system demonstrated high selectivity for S. aureus and was unaffected by other interfering microorganisms or food preservatives, highlighting its specificity and interference-resistance. Whole-genome sequencing of SapYZU04 suggested that the selectivity, sensitivity, and specificity of SapYZU04@MnFeO could be attributed to the tail fibre proteins of the phage. In conclusion, the SapYZU04@MnFeO biosensor is a highly effective tool for identifying viable S. aureus strains in the food industry.
研究领域:
微生物学
·
食品安全
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xiao-Jie Shi,Xin-Yi Dai,Yu Wu,Ting-Ting Zhang,Tian-Shun Song,Xiang-Ling Li,Jing-Jing Xie
Achieving a simple process, detailed information, and visual response for cancer diagnosis and treatment is desirable but challenging. Herein, we develop a self-cascade system-triggered colorimetric array for the sensitive detection of choline, a promising biomarker in triple-negative breast cancer (TNBC). The system utilizes a ChOx-Ce 3+ probe, where ChOx catalyzes the oxidation of target choline to betaine, generating H 2 O 2 , which then in situ reacts with Ce 3+ to form a CeO 2 nanozyme. This nanozyme exhibits robust oxidase (OXD)-like activity, oxidizing tetramethylbenzidine (TMB) to oxTMB, resulting in a visible color change that correlates with choline concentration. The self-triggered cascade mechanism significantly simplifies the detection process by eliminating the need for expensive materials and complex equipment. Additionally, it enhances the selectivity, resistance to protein interference, and long-term stability of the probe. The system demonstrated high accuracy in measuring choline concentrations in MDA-MB-231 cells and real samples, with recovery rates ranging from 97.4 % to 101 %. These results illustrate the potential of the self-cascade system-triggered colorimetric array for early TNBC detection and therapeutic monitoring, offering a cost-effective, user-friendly diagnostic tool for clinical applications.
研究领域:
生物化学
·
癌症诊断
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 66
·38568-38575
·
IF 3.049
Jinping Song,Qi Ma,Yequn Liu,Yong Guo,Feng Feng,Shaomin Shuang
In this study, dual-emission carbon dots (D-CDs) are synthesized via a simple one-step solvothermal treatment of red tea. The obtained D-CDs are characterized by XPS, IR, TEM, XRD, fluorescence and UV-vis spectroscopy techniques. It is found that D-CDs present a strong red fluorescence emission peak at 671 nm and weak blue fluorescence emission peak at 478 nm under the excitation wavelength of 410 nm. The unique dual-emission properties of D-CDs provide great opportunities in ratiometric fluorescence sensing applications. The results show that Cu2+ ions can quench the fluorescence of the red emission band of D-CDs effectively, resulting in the disappearance of red fluorescence ultimately. Upon the addition of Al3+ ions, the fluorescence of blue emission band at 478 nm grows apparently, and the fluorescence color transforms gradually from red to orange, then to yellow-green. Based on these findings, a novel ratiometric fluorescence and colorimetric dual mode nanosensor is developed for simultaneous detection of Cu2+ and Al3+ ions. Regarding Cu2+ ions, the fluorescent detection linear range is 0.1–50 μM with detection limit of 0.1 μM, and the colorimetric detection limit is estimated as 25 μM. With regard to Al3+ ions, the fluorescent detection linear range is 0–20 μM and 25–100 μM with detection limit of 0.5 μM, and the colorimetric detection limit is 20 μM. Furthermore, the fluorescence response mechanisms of Cu2+ and Al3+ ions were discussed detailed. To the best of our current knowledge, this will be the first research work on the simultaneous determination of Cu2+ and Al3+ using D-CDs as fluorescent probes.
研究领域:
分析化学
·
纳米材料科学
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2020
·
IF 3.233
Biao Zheng,Jing Li,Zhongkai Zheng,Cheng Zhang,Chunlei Huang,Jinquan Hong,Yuliang Li,Jun Wang
A sensitive and selective method for the colorimetric detection of arsenic (As III ) ions has been demonstrated using glutathione functionalized gold nanoparticles (GSH-AuNPs). When arsenic ions are added to the GSH-AuNPs solution, the binding of arsenic ions with GSH ligands induces the aggregation of AuNPs, resulting in a rapid color change of the solution. With the implementation of a home-developed RGB extracting software, excellent linear correlation has been established between the calculated RGB color values and the arsenic concentrations ( R 2 = 0.99488). The limit of detection (LOD) of arsenic ions is as low as 0.12 ppb. More significantly, the GSH-AuNPs solution shows excellent detection selectivity to other metal ions (Fe 3+ , Co 2+ , Ni 2+ , K + , Na + , Mg 2+ , Ca 2+ , Cu 2+ , Hg 2+ , and Pb 2+ ). This method could be applied to on-site and real-time fast detection of low level arsenic ions.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Xin Zhang,Xiu Gong,Binbin Shang
Colorimetric concentration detection based on photonic crystals has attracted considerable attention owing to the substantial color changes in response to external stimuli. In general, photonic crystals for colorimetric detection are carefully designed by introducing tailored recognition sites into an ordered structure. These materials are widely used in the detection of trace substances. However, the traditional photonic crystal assembled by colloid particles was rarely applied in concentration detection since the solute tends to occupy the interparticle voids, causing reduced refractive index contrasts and closed bandgaps at higher concentrations. Consequently, the development of a more convenient and universal concentration detection material is an urgent requirement. Herein, photonic crystals assembled by mesoporous SiO2 particles (m-SiO2–PC) were prepared and used as a colorimetric indicator of solution concentration for nonvolatilized substances. The solute preferentially occupied the mesopores of m-SiO2 particles, yielding an increase in reflection index, a shift in reflection peak, and considerable color contrasts on the m-SiO2–PC film after the evaporation of solvent. The relation between wavelength shifts and solution concentrations could be used for quantitative concentration detection. A colorimetric concentration indicator based on m-SiO2–PC film provides a more efficient and universal method that is applicable for a variety of solutions.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Hao-Hua Deng,Bang-Yue Luo,Shao-Bin He,Rui-Ting Chen,Zhen Lin,Hua-Ping Peng,Xing-Hua Xia,Wei Chen
Although it has been demonstrated that rare-earth elements (REEs) disturb and alter the catalytic activity of numerous natural enzymes, their effects on nanomaterial-based artificial enzymes (nanozymes) have been seldom explored. In this work, the influence of REEs on the peroxidase-like activity of bare gold nanoparticles (GNPs) is investigated for the first time, and a new type of Ce3+-activated peroxidase mimetic activity of GNPs is obtained. The introduced Ce3+ can be bound to the bare GNP surface rapidly through electrostatic attraction, after which it donates its electron to the bare GNP. As H2O2 is a good electron scavenger, more •OH radicals are generated on the surfaces of the bare GNPs, which can considerably enhance TMB oxidation. Due to its redox cycling ability, the activation effect of Ce3+ is proved to be more efficient in comparison to those of the other reported metal ion activators (e.g., Bi3+, Hg2+, and Pb2+). In addition, it is determined that Ce3+ should directly contact with the gold core to trigger its activation effect. When the surface states of the bare GNPs are altered, the Ce3+-stimulated effect is strongly inhibited. Furthermore, a novel colorimetric method for Ce3+ is developed, on the basis of its enhancing effect on the peroxidase mimetic activity of bare GNPs. The sensitivity of this newly developed method for Ce3+ is excellent with a limit of detection as low as 2.2 nM. This study not only provides an effective GNP-based peroxidase mimic but also contributes in realizing new applications for nanozymes.
研究领域:
分析化学
·
纳米生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Le Dong,Ruyu Li,Liqiu Wang,Xifa Lan,Haotian Sun,Yu Zhao,Longgang Wang
The sensitive colorimetric detection of glucose using nanomaterials has been attracting considerable attention. To improve the detection sensitivity, highly stable lentinan stabilized platinum nanoclusters (Pt-LNT NCs) were prepared, in which lentinan was employed as a mild reductant and stabilizer. The size of platinum nanoclusters (Pt NCs) was only 1.20 ± 0.29 nm. Pt-LNT NCs catalyzed the oxidation of substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H 2 O 2 ) to produce a blue oxidation product with absorption peak at 652 nm, indicating their peroxidase-like properties. Their enzymatic kinetics followed typical Michaelis-Menten theory. In addition, fluorescence experiments confirmed their ability to efficiently catalyze the decomposition of H 2 O 2 to generate •OH, which resulted in the peroxidase-like mechanism of Pt-LNT NCs. Moreover, a colorimetric method for highly selective and sensitive detection of glucose was established by using Pt-LNT NCs and glucose oxidase. The linear range of glucose detection was 5–1000 μM and the detection limit was 1.79 μM. Finally, this method was further used for detection of glucose in human serum and human urine. The established colorimetric method may promote the development of biological detection and environmental chemistry in the future.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Shu Xing,Xiaojun Xu,Pan Fu,Mengjia Xu,Tingting Gao,Xiaokang Zhang,Chao Zhao
Rapid and sensitive single nucleotide polymorphisms (SNPs) genotyping is of particular important for early diagnosis, prevention, and treatment of specific human diseases. A simple and low-cost SNP detection method would be valuable for routine analysis in resource-limited settings. Here, we demonstrated a novel and convenient gold nanoparticle (AuNPs) based colorimetric approach for efficient screening of SNPs at room temperature without instrumentation. SNP detection is performed in a single tube with one set of unmodified AuNPs, a label-free peptide nucleic acid (PNA) probe, a single exonuclease (S1 nuclease), and the target to be tested. S1 nuclease could digest DNAs in DNA/PNA duplexes involving a mismatch into small fragments, while DNAs in the fully-matched DNA/PNA duplexes can be effectively protected by PNA from enzymatic degradation. This difference could be easily discriminated by color changes associated with gold aggregation. PNA oligomers can induce immediate AuNP aggregation even in the presence of nucleoside monophosphates (dNMPs), the digestion products of DNA . Whereas PNA/DNA duplexes can effectively stabilize unmodified AuNPs, and the stabilization effect of PNA/DNA is better than single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Without the need of precise temperature control and extra salt addition, SNPs are detected with a detection limit of 2.3 nM in cell lysate. Moreover, this system can effectively discriminate a range of different mismatches even in spiked cell lysate, demonstrate the potential use of this biosensor for biological samples.
研究领域:
分子生物学
·
纳米生物技术
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yan Zhao,Yiqun Zheng,Caiyun Zhao,Jinmao You,Fengli Qu
A novel colorimetric immunoassay was designed for the sensitive detection of carbohydrate antigen 125 (CA125). The success of this immunoassay relies on the use of hollow polydopamine-gold nanoparticles (PDA-Au) for signal amplification to achieve sensitive nonenzymatic colorimetric detection. In particular, PDA-Au was used as a stable and sensitive label and aminated-Fe 3 O 4 magnetic nanoparticles (Fe 3 O 4 NPs) were employed to immobilize capture antibody (Ab 1 ) and acted as a separable immunosensing probe. PDA-Au exhibited high catalytic performance towards p -nitrophenol reduction and thus resulted in significant color change and UV/vis signal variations. The immunoassay was performed based on sandwich protocol. As compared to pure Au nanoparticles, the signal amplification and sensitivity of PDA-Au-based assay was significantly improved. For instance, the dynamic range of the developed colorimetric immunoassay for CA125 was 0.1–100 U/mL with a detection limit of 0.1 U/mL at S / N =3. In addition, this immunoassay was also tested for the analysis of clinical serum samples, which demonstrated its potential for practical diagnostic applications.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yunshan Zhang,Tuo Huang,Fang Yang,Qianglong Tan,Jing Ye,Xianzhong Feng,Diming Zhang
In recent years, entropy-driven circuit (EDC) dynamic DNA networks have garnered significant attention in nucleic acid detection owing to their simplicity, efficiency, and flexible design. Nevertheless, conventional EDC reactions face a constraint in achieving optimal signal amplification due to a solitary and feeble driving force. To overcome this limitation, we innovatively devised a gold nanoparticle (AuNP) dispersion-enhanced EDC (Au-EDC) approach, pioneering a novel colorimetric signal amplification and output system. The system was harmoniously integrated with the ligase chain reaction (LCR) for precise single nucleotide polymorphism (SNP) genotyping. Specifically, LCR was selectively executed solely on the positive strand of the mutant target (MT), facilitating precise point-to-strand information transduction. Subsequently, the LCR product triggered the Au-EDC cycling reaction, causing the DNA-AuNPs network to progressively disintegrate and release a pronounced colorimetric signal. This strategic design ingeniously harnessed the entropy increase that occurs as AuNPs undergo a transition from aggregated to dispersed states, offering a supplemental impetus for the EDC cycle. The integrated LCR-Au-EDC system excelled in detecting MT at concentrations as low as 320 fM and differentiating pooled samples with mutation frequencies as low as 0.1%. Moreover, the system accurately performed SNP genotyping on the real genomes derived from soybean leaves. Consequently, this study not only develops a colorimetric signal amplification and output sensing system based on EDC reactions but also provides a cost-effective and efficient SNP genotyping tool.
研究领域:
生物化学
·
分子生物学
·
纳米技术
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）