TALANTA
·
2024
·
IF 5.6
Xiaoming Chen,Shuo Cao,Liye Tao,Runlan Yan,Sheng Cao,Jingwen Hao,Yuelin Yi,Chunyan Luan,Jianmin Wu,Yue Gao,Xiao Liang
Lipidomics has demonstrated significant potential for disease diagnosis and prediction. The development and optimization of a robust mass spectrometry (MS) platform for lipidome analysis is critically important, as it can facilitate biomarker discovery, cohort testing, and performance evaluation in clinical lipidomics studies. In this work, we developed a high-throughput and reliable platform, termed MS Lab on a Chip ( MS LOC ), which integrates the MetArray chip, an automated lipidomics pretreatment protocol, and the reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) instrument. The MetArray chip, produced through a mass production process, exhibited exceptional stability as an MS substrate. The integration of automated lipid pretreatment and MS detection processes ensures high throughput, stability and efficiency during sample preparation. The analysis of various lipid standards and different types of biological samples enabled comprehensive investigation of lipid features and annotation using the MS LOC . Furthermore, a small cohort study, consisting of hepatocellular carcinoma (HCC) and non-HCC groups, was conducted on this platform, providing preliminary validation of its performance and suggesting that this platform offers a comprehensive protocol for clinical lipidomics testing.
研究领域:
分析化学
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临床研究
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质谱技术
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组学技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Nan Zhao,Hao Wang,Jiarui Li,Xi Lin,Liming Guo,Xinhua Guo
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is emerging as the most powerful tool for the identification of bacteria. Herein, we demonstrated that the capability of MALDI-TOF-MS towards the discrimination of clinical bacteria can be further dramatically improved. A cooperative matrix consisting of (E)-propyl α-cyano-4-hydroxylcinnamylate (CHCA-C3) and α-cyano-4-hydroxycinnamic acid (CHCA) was used to replace the commonly used matrix CHCA. As a result, 1.4–3.8 times more fingerprint peaks have been identified by the cooperative matrix in comparison with CHCA when six different bacteria were studied. The peaks distribute in the wider m / z range, and the spectral quality has also been significantly improved. As an example of practical applications, the direct and more reliable discrimination between Escherichia coli (E. coli) and Shigella sonnei ( S. sonnei) was achieved based on their protein fingerprints, which was previously claimed to be impossible by CHCA. Thus, a promoted MALDI-TOF-MS platform towards bacterial identification has been established by utilizing the new cooperative matrix, which will exhibit great potential for many applications.
研究领域:
分析化学
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微生物学
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质谱学
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Yuean Chu,Mingyue Yu,Yunxing Li,Suoxian Yang,Hongyan Bi
Tracement of the origin of seafoods and aquatic products is an important issue for avoiding the falsification of production regions of aquatic products. Herein, by analyzing black tiger shrimp ( Penaeus monodon ) as a model of aquatic products, we demonstrated a method to trace the origin of aquatic products by analyzing the gut microbiome of black tiger shrimp ( Penaeus monodon ) collected from three commercial farms in China, via matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Multivariate analysis was executed on the obtained mass spectrometric datasets of bacteria extracted and collected from shrimp samples with different production regions. The gut microorganisms of black tiger shrimps can be identified by scoring the gut microbiota of black tiger shrimps collected from three producing areas. The black tiger shrimp from three production regions were found with distinguished characteristics of bacterial prevalence in their digestive gland microbiota. The dominant bacteria genus in digestive gland microbiota from black tiger shrimp samples from the three production regions are completely different. Single-blind shrimp samples with unknown production region was successfully screened by comparing the detected MS data in different ways. This strategy can trace the production region of the analyzed shrimp samples in a quick and efficient mode. The application of the present protocol was further demonstrated by analyzing the biometric species from gill tissue of Chinese mitten crab ( Eriocheir sinensis ) samples collected from crab-producing areas in Jiangsu Province (China), that is, Taihu Lake, Gaoyou Lake, and Hongze Lake for tracing their production area. It is expected to extend the presently developed method to provide clues for the origin traceability of other aquatic products.
研究领域:
水产养殖
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食品科学
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微生物学
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质谱分析
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yanbo Hu,Yiming Li,Yuzhu Shen,Baochun Zhang,Jiaxin Liu,Yi Cao,Jun Zhao
In this study, six rare protopanaxadiol (PPD)-type ginsenosides, namely, F2, CK, C-Mc1, C-Mc, CO, and CY, were prepared using enzymatic hydrolysis. Firstly, major (PPD)-type ginsenosides, including Rb1, Rb2, Rc, and Rd, were biotransformed by employing single and combined β-glucosidases BaBgl1A and BaBgl3A. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses revealed that ginsenosides F2, CO, and C-Mc1 could be produced by BaBgl1A, while rare ginsenosides CK, CY, and C-Mc could be produced by the combined use of BaBgl1A and BaBgl3A. Furthermore, optimal preparation conditions for these rare ginsenosides were elucidated, with yields exceeding 76.34%. Additionally, large quantities of rare ginsenosides were prepared to determine their structures. Characteristic absorption peaks in 13 C NMR spectra and ion peaks in MALDI-TOF-MS suggested that the six high-purity rare ginsenosides (i.e., F2, CO and C-Mc1, CK, CY, and C-Mc) could be produced at high-efficiency. The findings of this study offer insights into novel enzymolysis methods for the targeted preparation of rare PPD-type ginsenosides, thus widening the range of raw materials that could be directed to in-depth biological activity research.
研究领域:
生物化学
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药理学
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生物技术
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 45
·期 1
·123-130
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IF 3.288
Wei Gao,Pingping Jiang,Qian Gu,Hong Zhang,Pingbo Zhang,Agus Haryono
A novel green plasticizer, acetylated lactic acid 1,6-hexane diester (ALHD), was successfully synthesized based on L-lactic acid, a renewable organic acid from corn starch. Its chemical structure was characterized using FTIR, 1H NMR and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The plasticizing effects of ALHD as an alternative substitute for commercial plasticizer acetyl tributyl citrate (ATBC) in poly(vinyl chloride) (PVC) were investigated in detail. The results show that the PVC films plasticized using ALHD have better organic solvent resistance and food mimic migration stability than ATBC. After 24 hours of extraction in cyclohexane and petroleum ether, the weight loss of PVC sample S5 plasticized using pure ALHD was reduced by 83.6% and 77.5%, respectively, compared to that of PVC sample S1 plasticized with pure ATBC. Furthermore, after 72 hours of migration in activated carbon, the weight loss of PVC sample S5 decreased by 44.0% compared to that of PVC sample S1, which indicated that ALHD had excellent volatility and migration stability. The elongation at break of the PVC pieces increased from 644.65% to 802.15% as the content of ALHD improved, showing an increase of 24.4%. The mechanical property analysis showed that, with the replacement of ALHD in the place of ATBC, the PVC blends exhibited better flexibility and elasticity.
研究领域:
材料科学
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化学
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环境科学
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高分子科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）