FOOD CONTROL
·
2025
·
IF 6.3
Zhang-Na Qin,Zhen-Peng Zhen,Yu-Feng Gao
In this study, a high-throughput matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) method was developed for detecting three furfurals (furfural, 5-methylfurfural, and 5-hydroxymethylfurfural) in food. The derivatization of furfurals with 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) resulted in a substantial increase in the m/z values of furfurals and an improvement in detection sensitivity. The compound 5-methoxymethylfurfural, a structural analogue of furfurals, was utilized as an internal standard to correct for fluctuations in mass spectrometry signals and matrix effects present in the real samples. The calibration curves exhibited good linearity in the furfural concentration range of 0.5–50 μg/mL, with correlation coefficients (R) exceeding 0.9996. The limits of quantification were in the range of 0.20–0.39 μg/mL. The furfural recoveries ranged from 89.2% to 116.2%, and the intra- and inter-day precisions were less than 10.8%, indicating high detection accuracy and precision. The developed method was successfully applied to the detection of furfurals in a variety of real samples, including roasted coffee beans, honey, and vinegar. The results demonstrated that the developed method was rapid and high-throughput, meeting the requirements for accurately detecting furfurals in various foods.
研究领域:
分析化学
·
食品科学
·
质谱技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Shuo Xing,Jinli Ma,Fudong Zeng,Liangqiang Wu,Yixuan Yao,Yapeng Li,Jianshi Du,Qingbiao Yang,Yaoxian Li
Zinc is one of the essential trace elements in the human body and plays an extremely important role in physiological processes. Therefore, it is necessary to develop a detection method that can be used for real-time, highly sensitive response of Zn 2+ in organisms and has low toxicity. In this work, a novel fluorescent probe NA for Zn 2+ detection based on 1,8-naphthalene dicarboxylic anhydride and dimethylpyridine amine was synthesized. Probe NA has specific recognition and real-time monitoring functions for Zn 2+ . Based on Job's plotting curve, it is inferred that the coordination ratio between probe NA and Zn 2+ is 3:1, forming a complex of [Zn(NA) 3 ] 2+ . The [Zn(NA) 3 ] 2+ exhibits obvious yellow fluorescence at 550 nm. Probe NA can achieve quantitative detection of Zn 2+ with a detection limit of 7.52 × 10 −9 M, with high selectivity and anti-interference ability. Probe NA can stably exist in a wide pH range (2–10). This indicates that it can detect Zn 2+ in complex environments. In addition, based on the low cytotoxicity, high sensitivity, and real-time monitoring characteristics of probe NA, we have expanded it to real-time monitoring of Zn 2+ in organisms (HUVEC cells and live mice), as well as qualitative and quantitative detection of Zn 2+ concentration in food and drugs.
研究领域:
化学
·
生物学
·
医学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Jian-Jun Zhong,Ningbo Liao,Tian Ding,Xingqian Ye,Dong-Hong Liu
Direct separation of biogenic amines by reversed-phase liquid chromatography (RPLC) is not an easy task because their basic and hydrophilic characteristics can lead to poor retention, column overloading, peak tailing, and hence low efficiency. Rather than routinely resorting to derivatization or using classical hydrophobic ion-pair reagents (IPR), this work proposes a new RPLC method making use of the chaotropic salt KPF 6 as inorganic additive to an acidic acetonitrilic eluent to remedy the difficulties. Amine retention, overload behavior, peak shape, and column efficiency were significantly improved. The use of excess KPF 6 led to a very slight decrease of amine retention. Depending on amine, the dependence of the logarithmic retention factor on the volume percent of acetonitrile could be reasonably linear or quite convex. Coupled with UV detection, the method was applied to trace analysis for six biogenic, aromatic or heterocyclic amines in three types of food after a sample cleanup, as necessary, by ion-pair extraction. The reliability of the whole analysis was demonstrated to be satisfactory. The proposed method outperforms existing methods in that it eliminates the need for long and cumbersome derivatization procedures without losing sensitivity; it also represents a good surrogate for classical ion-pair chromatography (IPC) because of the desirable hydrophilicity of chaotropic salts.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 105
·86445-86452
·
IF 3.84
Zhenzhen Zhang,Lingling Wang,Xueyan Liu,Danfeng Zhang,Lei Zhang,Qi Li
A new micro-solid-phase extraction method based on a prestructured filter membrane was developed to facilitate simultaneous analysis of tartrazine (TA) and sunset yellow (SY) with high performance liquid chromatography (HPLC). A filter membrane impregnated with nano-Al2O3 particles was proposed as a new micro-solid-phase extraction (μ-SPE) device for the preconcentration of colorants in food. The method integrated isolation and concentration in one step, and the adsorbed colorants could be easily eluted from the filter membrane for HPLC analysis. This process does not involve any additional sample treatment. The method is facile and easy to operate. The method showed good linearity, with correlation coefficients >0.9982 in the range of 1.0–200.0 ng mL−1, and low limits of detection (0.10–0.20 ng mL−1). Finally, the recovery values were 86.9–124.0% for the colorants in various food samples. The results indicated that the developed method would be a novel method for rapid determination of TA and SY at trace levels in real samples.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Yao Sun,Zhaobing Guan,Hongwei Cai,Yiyong Huang,Yawei Lin,Xiaosong Hu
A pre-column fluorescent derivatization method based on nitrone formation has been applied to determine furfurals (e.g. furfural (F), 5-methyfurfural (5-MF) and 5-hydroxymethylfurfural (5-HMF)) in food samples for the first time. An N-substituted hydroxylamine reagent 4-((hydroxyamino)butyl)-7-hydroxycoumarin (HAHC) was used to react with the aldehyde group of furfurals to form stable nitrone derivatives with high fluorescence intensities . The reactions proceeded under mild conditions in 30 min with high derivatization yields (>93%). A baseline-separation of three furfurals derivatives was subsequently achieved within 25 min on a reversed-phase column. The detection limits were at the low femtomol level (S/N = 3, 20 μL per injection). The linear range of the calibration curve was 0.4–4000 nM with good correlation coefficients (R 2 ≥ 0.9991). The proposed method was further applied for food sample analysis, such as bovine milk powder and raisin. Satisfactory recoveries were obtained in the range of 94.7%–103.5%. Above all, this pre-column derivatization method is simple, fast and highly sensitive, providing an effective and promising way for future studies of aldehydes in different matrices.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Biling Huang,Lihua Xu,Jianxi Ying,Yufen Zhao,Shaohua Huang
Chiral carboxylic acids are ubiquitous in nature and are of pivotal importance in practical applications in the field of food science and pharmaceuticals. Simultaneous enantiomeric analysis of carboxylic acids mixtures is a significant research goal. Herein, we demonstrate a new strategy involving the use of the highly selective chiral derivatizing agent ( R )-2-amino-1,1,1-trifluoropropane (( R )-TFPA) for the enantiomeric discrimination and selective identification of carboxylic acids in mixtures, even structurally similar drugs. The key success of this sensing approach lies in the distinguishable 19 F NMR signals of diastereoisomers formed via derivatization of the substrates by ( R )-TFPA. The utility of this approach was demonstrated by simultaneously differentiating 20 chiral carboxylic acids in a complicated mixture with accurate determination of the enantiomeric excess ( ee ). Most importantly, our approach can be applied to food analysis, where the diverse carboxylic acids in real samples without separation were unambiguously identified, such as vinegar , yogurt, and grape.
研究领域:
食品科学
·
药物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Huang-Fei Jin,Ying Shi,Yan-Hua Jiao,Ting-Hong Fei,Jun Cao,Li-Hong Ye
A novel online two-step pressure injection-assisted stacking preconcentration method, which involves sweeping and affinity micelles in micellar electrokinetic chromatography was developed to simultaneously measure various organic anions. The micellar solution was a mixed solution that contained 0.3 mM didodecyldimethylammonium bromide and 20 mM borax. After the micellar solution was injected for 60 s, the tested analytes prepared in 20 mM borax were introduced into the capillary for 150 s. The key experimental factors that influenced the separation and sensitivity were investigated and optimized, including the concentration and injection time of the micellar solution, the concentration of borax in the sample solution, the concentration of sodium dodecyl sulfate and borax in the background electrolyte (BGE), the content of acetonitrile in the BGE and the injection time of the sample solution. Compared with typical injection methods, this method achieved sensitivity enhancement factors ranging from 85 to 97 under optimized conditions. Good linearity for matrix-matched calibration was established for all analytes with R 2 values of 0.9986–0.9996. The intraday ( n = 6) and interday ( n = 6) precisions of the method were less than 2.85% when expressed as relative standard deviations. When the method was applied to analyze rice and dried ginger samples, analyte recoveries ranged from 85.81% to 106.59%. Through sweeping and affinity micelles, stacking preconcentration method was successfully employed to analyze trace amounts of fenoprop and 2,4-dichlorophenoxyacetic acid in rice and dried ginger samples.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Sheng Wang,Jun Xu,Fengzhi Yue,Lina Zhang,Ning Bi,Jian Gou,Yongxin Li,Yuanyuan Huang,Tongqian Zhao,Lei Jia
Dipicolinic acid (DPA), as a biomarker for Bacillus anthracis , is highly toxic at trace levels. Rapid and on-site quantitative detection of DPA is essential for maintaining food safety and public health. This work develops a dual-channel self-calibrated fluorescence sensor constructed by the YVO 4 :Eu and Tb- β -diketone complex for rapid visual detection of DPA. This sensor exhibits high selectivity , fast response time, excellent detection sensitivity, and the detection limit is as low as 4.5 nM in the linear range of 0–16 μM. A smartphone APP and portable ultraviolet lamp can assemble a mobile fluorescence sensor for on-site analysis. Interestingly, adding Cu 2+ ions can quench the fluorescence intensity of Tb 3+ . In contrast, the addition of cysteine can restore the fluorescence, allowing the accurate detection of Cu 2+ ions and cysteine in environmental water and food samples. This work provides a portable sensor that facilitates real-time analysis of multiple targets in food and the environment.
研究领域:
生物化学
·
食品安全
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 81
·期 7
·1097-1102
·
IF 1.401
Zhong Jian-Jun,Liao Ningbo,Pu Yunfeng,Ding Tian,Ye Xingqian,Liu Donghong
Nowadays, there is a demand for fast liquid chromatography (LC) of biogenic amines in food with the equipment available in the average laboratory. To this end, high-temperature liquid chromatography (HTLC) is presented in this study as a viable option, working on a conventional LC platform at moderately high temperatures up to 80 °C. The LC platform was adapted by including an appropriate length of stainless-steel microbore tubing as a preheater. Biogenic amines as benzoyl derivatives showed good thermostability on a thermally rugged column (150 × 4.6 mm, 5 µm). As a model application the HTLC conditions were developed for wine separation. The separation selectivity changed considerably with temperature in the range 36–84 °C, and baseline resolution was obtained only at temperatures well above ambient. At 73 °C oven temperature, the system allowed a flow rate as high as 3 mL min −1 with the backpressure not exceeding 195 bar (2800 psi). The separation took less than 5 min, comparably fast to documented fast LC separations, and typically at least three to five times faster than a conventional separation.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROPHORESIS
·
2015
·Vol. 36
·期 20
·2509-2515
·
IF 3.028
Haiyun Zhai,Kaisong Yuan,Xiao Yu,Zuanguang Chen,Zhenping Liu,Zihao Su
A novel fluorescence detection system for CE was described and evaluated. Two miniature laser pointers were used as the excitation source. A Y-style optical fiber was used to transmit the excitation light and a four-branch optical fiber was used to collect the fluorescence. The optical fiber and optical filter were imported into a photomultiplier tube without any extra fixing device. A simplified PDMS detection cell was designed with guide channels through which the optical fibers were easily aligned to the detection window of separation capillary. According to different requirements, laser pointers and different filters were selected by simple switching and replacement. The fluorescence from four different directions was collected at the same detecting point. Thus, the sensitivity was enhanced without peak broadening. The fluorescence detection system was simple, compact, low-cost, and highly sensitive, with its functionality demonstrated by the separation and determination of red dyes and fluorescent whitening agents. The detection limit of rhodamine 6G was 7.7 nM (S/N = 3). The system was further applied to determine illegal food dyes. The CE system is potentially eligible for food safety analysis.
研究领域:
分析化学
·
生物医学工程
·
食品安全
·
分子检测技术
✓ 已验证
DOI
高影响因子
SENSORS
·
2018
·Vol. 18
·期 9
·2762
·
IF 2.475
Jingheng Ning,Quanguo He,Xin Luo,Min Wang,Donglin Liu,Jianhui Wang,Jun Liu,Guangli Li
A facile cuprous oxide nanoparticles functionalized electro-reduced graphene oxide modified glassy carbon electrode (denoted as Cu2O NPs-ERGO/GCE) was fabricated via a simple physical adsorption and electrochemical reduction approach. Cyclic voltammetry and second-order derivative linear scan voltammetry were used to investigate the electrocatalysis oxidation of vanillin on the Cu2O NPs-ERGO/GCE. The compound yielded a well-defined voltammetric response in 0.1 M H2SO4at 0.916 V (vs. saturated calomel electrode (SCE)). A linear calibration graph was obtained in the concentration range of 0.1 μM to 10 μM and 10 μM to 100 μM, while the detection limit (S/N = 3) is 10 nM. In addition, the Cu2O NPs-ERGO/GCE presented well anti-interference ability, stability, and reproducibility. It was used to detect vanillin sensitively and rapidly in different commercial food products, and the results were in agreement with the values obtained by high performance liquid chromatography.Keywords:cuprous oxide nanoparticles;electroreduced graphene oxide;vanillin;food analysis
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2023
·
IF 3.3
Liyun Yang,Naijia Jiang,Huiyan Wu,Zhouyang Li,Yi Liu,Zhiqiang Zhou
Development of a facile and effective strategy for highly sensitive detection of sulfite (SO 3 2− ) in food is of great significance to human health. Herein, fluorescent porphyrin-based MOFs (PCN-224) was synthesized and further developed as an enhanced fluorescence sensor for SO 3 2− detection. The fluorescence peak of PCN-224 locates at 664 nm. In the presence of SO 3 2− , the acid-base reaction between H 2 PO 4 − and SO 3 2− promotes the generation of PO 4 3− , thus forming Zr-OP bonds and enhancing the fluorescence intensity of PCN-224 at 664 nm. Therefore, H 2 PO 4 − is used as an auxiliary agent to construct the sensing system PCN-224/H 2 PO 4 − . The fluorescence enhancement of PCN-224/H 2 PO 4 − shows a good linear relationship in the range of 0.01–18 μM and the limit of detection is as low as 3.8 nM. In addition, the constructed PCN-224/H 2 PO 4 − sensor system can be successfully used for SO 3 2− detection in the granulated sugar with a recovery ranging from 100.17% to 106.25%, demonstrating the feasibility of PCN-224/H 2 PO 4 − nanosensor for SO 3 2− detection in real sample analysis.
研究领域:
化学传感器
·
食品安全
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）