FOOD CHEMISTRY
·
2021
·
IF 7.514
Lukai Ma,Qiuxuan He,Yuanxin Qiu,Huifan Liu,Jihong Wu,Guoqin Liu,Charles Brennan,Margaret Anne Brennan,Lixue Zhu
Vegetable oils are increasingly replacing animal fats in diets, but malondialdehyde (MDA), a peroxidation product of these oils, has been regarded as toxic; this necessitated investigation of MDA formation during consumption. This study investigated MDA formation in four vegetable oils during frying French fries (FF) and fried chicken breast meat (FCBM) at 180 °C for 7 h. Results showed that MDA contents were lower in oils used for frying foods than in control oils, mainly because MDA was incorporated into the foods. MDA content was lower in FF, but higher in FCBM, due to the different food components. Model oil and food system analyses yielded similar results. MDA bound the hydrophobic helical structure in starch-based FF, but was exhibited greater reactivity with nucleophilic groups in protein-based FCBM, resulting in stronger interaction with FCBM than with FF. Our results indicated the existence of distinct mechanisms underlying MDA migration in different food matrixes.
研究领域:
食品科学
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毒理学
·
营养学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Ya-Qian Wang,Li Li,Jin Yin,Xu Yu,Xiaowei Wu,Li Xu
In this study, silane reagents were for the first time explored as the fluorescence sensitizer. They were demonstrated to have fluorescence sensitization effect on curcumin and 3-glycidoxypropyltrimethoxysilane (GPTMS) possessed the strongest effect. Thus, GPTMS was adopted as the novel fluorescence sensitizer to turn on the fluorescence of curcumin by more than two orders of magnitude for detection. In this way, curcumin could be determined with a linear range of 0.2–2000 ng/mL and an LOD of 0.067 ng/mL. The method was applicable to determine curcumin in several actual food samples, which had the good consistency with the high performance liquid chromatographic method, demonstrating the high accuracy of the proposed method. In addition, the curcumins sensitized by GPTMS could be cured under certain conditions and held the potential for solid fluorescence application. This study expanded the scope of fluorescence sensitizer to silane reagents, and provided the novel approach for fluorescence detection of curcumin and further to generate new solid fluorescence system.
研究领域:
分析化学
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食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yuanchao Lu,Xiaohua Nie,Zeyi Wu,Chengyu Jin,Ningxiang Yu,Xianghe Meng,Qin Ye
Food matrices greatly impact TBBQ content during digestion, while lacking sufficient research and understanding. This study investigated the influence and mechanism of fried foods on the TBBQ-eliminated performance during in-vitro digestion. The results indicated that TBBQ content varied significantly among food matrices after in-vitro digestion, with the highest in peanuts (38.3%). The correlation analysis revealed that proteins remarkably facilitated TBBQ-eliminations while fats decreased the TBBQ-eliminated rate. The TBBQ-eliminated performance of proteins, protein digestive mixtures, and amino acids uncovered that sulfhydryl groups were crucial reactive groups to eliminate TBBQ, and TBBQ-eliminated rates under intestinal pH (8.0) were faster than gastric pH (1.5). Additionally, fats significantly reduced the protein-triggered TBBQ-eliminations, originating that the oil–water interface increased the interaction difficulty between lipophilic TBBQ and proteins. Thus, this work provided an in-depth understanding of food matrices (especially proteins and fats) in TBBQ eliminations to enlighten the promising TBBQ-risk-reduced strategies with high-protein and low-fat foods.
研究领域:
食品科学
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营养学
·
化学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 20
·3306
·
IF 5.561
Xiaofu Wang,Yunsong Zhao,Sufang Zhang,Xinping Lin,Huipeng Liang,Yingxi Chen,Chaofan Ji
Biogenic amines (BAs) are produced by microbial decarboxylation in various foods. Histamine and tyramine are recognized as the most toxic of all BAs. Applying degrading amine enzymes such as multicopper oxidase (MCO) is considered an effective method to reduce BAs in food systems. This study analyzed the characterization of heterologously expressed MCO fromL. sakeiLS. Towards the typical substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the optimal temperature and pH for recombinant MCO (rMCO) were 25 °C and 3.0, respectively, with the specific enzyme activity of 1.27 U/mg. Then, the effect of different environmental factors on the degrading activity of MCO towards two kinds of BAs was investigated. The degradation activity of rMCO is independent of exogenous copper and mediators. Additionally, the oxidation ability of rMCO was improved for histamine and tyramine with an increased NaCl concentration. Several food matrices could influence the amine-oxidizing activity of rMCO. Although the histamine-degrading activities of rMCO were affected, this enzyme reached a degradation rate of 28.1% in the presence of surimi. Grape juice improved the tyramine degradation activity of rMCO by up to 31.18%. These characteristics of rMCO indicate that this enzyme would be a good candidate for degrading toxic biogenic amines in food systems.Keywords:Lactobacillus sakei;biodegradation;multicopper oxidase;food matrices;biogenic amines
研究领域:
食品科学
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酶学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Ji Wang,Huihui Li,Changbin Li,Yifeng Ding,Yuanshang Wang,Wenjuan Zhu,Jia Wang,Yanchun Shao,Hui Pan,Xiaohong Wang
Recently, using bacteriophages as new molecular probes in reliable platforms for the detection of bacterial pathogens has attracted more and more increasing attentions. In this paper, a novel isolated Myoviridae bacteriophage SEP37 was covalently immobilized onto gold nanoparticles (AuNPs) modified gold disk electrode (GDE) surfaces using cysteamine (Cys) as a crosslinker. Substrates of GDE-AuNPs-Cys-Phage SEP37 and specific capture of Salmonella cells had been characterized using scanning electron microscopy (SEM) separately. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to study the electrochemical response of the biosensor interface manufacturing and bacterial capture process. Under the optimal experimental conditions, this phage-based EIS biosensor was able to detect Salmonella with a wide linear range from 2 × 10 1 to 2 × 10 6 colony forming unit (CFU)/mL within 30 min in spiked lake water and lettuce samples, with a limit of detection (LOD) of 17 CFU/mL. The detection linear range of spiked chicken samples was 2 × 10 2 to 2 × 10 5 CFU/mL, with a LOD of 1.3 × 10 2 CFU/mL. In combination with a pre-enrichment process for 3.5 h, this assay could reach a LOD of 1 CFU/mL in chicken breast meat samples. Besides, this phage-based EIS biosensor provided good reproducibility and stability. This phage-based EIS biosensor opens a new opportunity for the detection of pathogenic bacteria using the inherent selectivity of bacteriophage recognition.
研究领域:
微生物学
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食品安全
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-12
·
IF 5.3
Li Na,Guo Yiqun,Wu Mengmeng,Wang Aiying,Guo Yuna,Li Yuancheng
A highly specific and ultrasensitive electrochemical immunosensor for malachite green (MG) detection in complex food matrices is presented. The sensor was constructed through a stepwise assembly process incorporating gold nanoparticles, antibodies, and metal–organic framework (MOF)-derived Pd/CuO@NiO nanocomposites. Its structure and electrochemical performance were thoroughly validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Key detection parameters, including pH and nanocomposite concentration, were systematically optimized to maximize sensor performance. The sensor demonstrated a broad linear detection range (10 −4 to 100 ng/mL) and an ultralow detection limit (42 pg/L). Specificity tests confirmed the immunosensor’s ability to selectively detect MG without interference from structurally similar compounds such as crystal violet, nitrofuran drugs, and chloramphenicol. Its practical applicability was verified using pretreated freshwater fish samples spiked with MG, yielding recoveries of 95%–105%. Furthermore, the results showed strong agreement with those of the conventional enzyme-linked immunosorbent assays (ELISA) method, with minimal difference ratios observed. These findings establish the immunosensor as a reliable, accurate, and rapid tool for detecting MG in food safety applications. Looking ahead, the platform’s modular design and versatility provide opportunities to extend its application to other harmful contaminants in food and environmental monitoring, contributing to broader advancements in public health and safety. Graphical abstract
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Lijun Wang,Hong Zhou,Haixia Hu,Xue Wu,Wenhao Guo,Yan Liu,Yukun Huang,Xiao Yang,Xianggui Chen
Nanozyme-based detection methods have gained increasing attention in the field of food safety. However, the effect of the food matrix on the nanozyme activity requires tedious sample pre-treatment for nanozyme-based detection. Therefore, a difunctional histidine-modified magnetic hybrid nanozymes (His-Fe 3 O 4 @Cu) was used as capture probes and signal amplifiers to develop a simple colorimetric aptasensor for Salmonella Typhimurium detection. The prepared His-Fe 3 O 4 @Cu nanozymes demonstrated a 5.0-fold increase in peroxidase-like activity compared to Fe 3 O 4 nanozymes and exhibited superparamagnetism with saturation magnetization values of 63.5 emu/g. By virtue of the strong superparamagnetism of the His-Fe 3 O 4 @Cu nanozymes, aptamer-conjugated His-Fe 3 O 4 @Cu nanozymes first enriched and separated S. Typhimurium from food matrix. Then, aptamer-conjugated His-Fe 3 O 4 @Cu nanozymes employed an etching solution to generate a detection signal via the peroxidase-like activity. In optimal conditions, this proposed colorimetric aptasensor successfully detected S. Typhimurium with a limit of detection (LOD) of 8 CFU/mL in a linear range of 1.2 × 10 1 to 1.2 × 10 6 CFU/mL. Finally, the proposed aptasensor was further validated in three actual samples, with a capture efficiency ranging from 88.6 % to 96.5 % and recoveries between 88.2 % and 104 %. These results revealed the significant potential of this method for S. Typhimurium detection in food samples.
研究领域:
食品安全
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生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ruiyu Fu,Xiaoqin Hai,Qingxin Lu,Hongbo Li,Jiaxiao Niu,Yanhui Zhang,Tingze Ren,Xiaoli Guo,Xin Di
A molecular-imprinted polymer (MIP) gel with high effective recognition of amphenicol antibiotics was synthesized for the first time based on layered double hydroxide (LDH) as the support and initiator, and functionalized β-cyclodextrin (β-CD) as the functional monomer. The synergistic effect of molecular imprinting recognition and β-CD host-guest affinity enabled MIP gel to exhibit excellent selectivity (imprinted factors: 3.9–9.4) and high adsorption capacity (28.9–75.4 mg g −1 ) for amphenicol antibiotics. Different adsorption isotherms and kinetics models were followed, suggesting heterogeneous single-layer recognition and chemical adsorption. After 5 cycles of adsorption and desorption, the adsorption capacity of MIP gel retained above 83.6 %, demonstrating favorable reproducibility and stability. Under optimal conditions, the method validation showed a satisfactory limit of detection (5–10 μg L −1 ), good correlation (r 2 > 0.9967), and respectable recovery (82.6–105.3 %). The MIP gel was applied to extract amphenicol antibiotics from food matrices, achieving recoveries in the range of 78.3–104.5 %. Importantly, the recognition mechanism was studied in detail using density functional theory. Therefore, the established method demonstrates high sensitivity and can be applied as a new tactic for detecting amphenicol antibiotics in food matrices.
研究领域:
分析化学
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食品安全
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Lei Cheng,Rong Huang,Qiang Cao,Na Liu,Peiyi Li,Mengmeng Sun,Haiyang Qin,Lidong Wu
Azo pigments with azo functional groups (–N N–) are commonly used in foods. Through rational design, here we report synthesis of a novel magnetic metal–organic framework (MOF-545) and the use of Fe 3 O 4 @MOF-545 for the selective separation of azo pigments from beverages. We find that Fe 3 O 4 @MOF-545 exhibits excellent adsorption/removal capacities for new coccine (NC, 459 mg g −1 ) and lemon yellow (LY, 476 mg g −1 ) individually. The results indicated that the adsorption capacities of Fe 3 O 4 @MOF-545 were 5 times higher than reported previously. The large surface area (120.4 m 2 /g) and the suitable pore size (1.6 nm) of Fe 3 O 4 @MOF-545 provided multiple accessible channels for azo pigment adsorption. The adsorption kinetics, stability, recovery, and reusability of Fe 3 O 4 @MOF-545 were all reported. The recoveries ranged from 92.9 to 104.5%, reflecting the capacity of MOF-545 to efficiently separate NC and LY. Fe 3 O 4 @MOF-545 is suitable for azo pigment determination and separation in food matrices.
研究领域:
材料科学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Meirong Niu,Xujun Pu,Xian Chen,Dabing Ren,Cheng Zhang,Lunzhao Yi,Yukun Yang,Ying Gu,Shuo Wang
Minimizing sample pretreatment while maintaining specific analyte binding and eliminating non-target interference from food matrices remains challenging in food safety analysis. Here, we fabricated an antifouling surface using a dopamine-triggered polyzwitterion co polymer, polydopamine-poly(carboxybetaine methacrylate-co-N-(2-aminoethyl) methacrylamide hydrochloride) [pDA-p(CBMA90-co-AEMA10)], via in-situ polymerization and the Michael addition reaction. Compared with homopolymers that were prepared using a single carboxybetaine methacrylate (CBMA), the copolymer formed by regulating the proportions of CBMA and (2-aminoethyl)methacrylamide hydrochloride monomers outperformed in terms of antifouling ability. Thiol-modified aptamers were subsequently immobilized on the polydopamine layer via Michael addition to construct an electrochemical sensor for aflatoxin B1 (AFB1). The sensor exhibited strong antifouling performance in various simulated and real food matrices, with signal suppression as low as 0.54 %. It also achieved a low detection limit of 0.19 pg mL −1 and enabled sensitive AFB1 detection using a simple sample pretreatment method, with recovery rates ranging from 94.6 % to 105.8 %.
研究领域:
食品安全分析
·
材料科学
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Jie Chen,Jinyi Chen,Juan Zhang
In this study, a hydrophobic NH 2 -UiO-66@NaOA composite was developed as an advanced SPME coating for efficient extraction and sensitive detection of polycyclic aromatic hydrocarbons (PAHs) in environmental and food matrices. Surface functionalization with sodium oleate (NaOA) effectively addressed the moisture resistance limitations of conventional MOFs, forming a stable hydrophobic layer while maintaining crystalline integrity. The modified material exhibited enhanced PAHs extraction efficiency through synergistic π-π stacking and hydrophobic interactions. Combined with GC-FID analysis, the optimized method demonstrated excellent sensitivity (LODs: 0.014–0.083 ng mL −1 ), wide linear range (0.2–200 ng mL −1 , R 2 > 0.99), and good reproducibility (RSD ≤ 7.71 %). Successful application was demonstrated in environmental water samples (lake water, wastewater) and food matrices (honey, black tea), showing reliable performance in complex sample analysis. The developed modification strategy showed broad applicability, as confirmed through successful extension to other MOF structures (ZIF-8, NH 2 -MIL-88). These results highlight the composite's potential as a robust SPME coating for monitoring trace-level persistent organic pollutants in environment and food safety applications.
研究领域:
分析化学
·
环境科学
·
食品安全
·
材料科学
·
污染物检测
·
色谱分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Youxiong Zhang,Qingping Wu,Ming Sun,Jumei Zhang,Shuping Mo,Juan Wang,Xianhu Wei,Jianling Bai
Herein, a facile and versatile label-free aptamer-based fluorescent assay for simple and sensitive detection of food allergen in food matrix is developed based on the functionalized magnetic nanoparticles (MNPs) as separation carrier and commercially available OliGreen dye as signal probe. First, the aptamer hybridized with capture probe that was conjugated on the surface of MNPs to form aptamer-MNPs complexes as detection probes, which undergo conformational change upon interaction with target analytes, resulting in the release of aptamer from the surface of MNPs. So the released aptamer in the supernatant produced a significant fluorescence enhancement signal by introducing the OliGreen dye based on its ultrasensitive and specific fluorescence enhancement upon binding to ssDNA. As proof-of concept, this proposed strategy was applied to quantitative detection of tropomyosin, one of the major allergen found in shellfish. Under optimal conditions, the linear range was from 0.4 to 5 μg mL −1 (R 2 = 0.996) with a low limit of detection calculated to be 77 ng mL −1 . Moreover, the aptamer-based assay had remarkable selectivity and was successfully applied in the detection of tropomyosin in food matrix. Due to its simple design, easy operation and high sensitivity and selectivity, the proposed aptamer assay could expect to expand applications in various allergens detection by changing corresponding aptamers on the detection probe.
研究领域:
食品科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）