Micro & Nano Letters
·
2018
·Vol. 13
·期 6
·868-871
·
IF 0.841
Yan-meng Li,Li-wei Wang,Ruo-ping Li,Jun-he Han,Ming-ju Huang
Malachite green (C 23 H 25 ClN 2 ) is an illegal fungicide of aquatic products due to its potential carcinogenic, teratogenic, and mutagenic effects. It is typically challenging to detect C 23 H 25 ClN 2 in fish with conventional techniques. The study reported a simple and rapid method for detecting C 23 H 25 ClN 2 on fish surface with surface-enhanced Raman scattering (SERS). Using the seed growth method with trometamol as the modifier, 110 nm in-diameter gold nanoparticles on thin glass as SERS substrate were prepared, which exhibited high SERS activity, reproducibility, stability, and flexibility in SERS measurement. The fish samples required no pretreatment and the entire measurement took <5 min. Quantitative analyses were also conducted. Excellent linear relationships ( R 2 = 0.9397–0.9977) were obtained between the concentrations and the peak intensities of C 23 H 25 ClN 2 at some characteristic peaks with a wide concentrations range (0.5–10 μM). The detection limit can reach 0.5 μM. This method provided an easy and fast way to detect C 23 H 25 ClN 2 on fish surface for food safety.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 10
·2483
·
IF 4.35
Wenzhi Tang,Yangchun Qi,Zhonghong Li
Food analysis based on the enzyme-linked immunosorbent assay (ELISA) is simple, sensitive and rapid, but requires a costly colorimetric instrument. The aim of this work was to develop a portable, low-cost and user-friendly colorimetric instrument for colorimetric ELISA and aflatoxin B1(AFB1) detection. The principle of the developed instrument was employing a light-emitting diode to generate the signal light and using a light-dependent resistor to measure the signal light absorbed by the oxidized 3,3′,5,5′-tetramethyl benzidine. The absorption spectra revealed that the solution absorbed signal light more strongly after reaction with H2SO4, and blue light would be favorably absorbed. Evaluations on the stability and accuracy of the instrument and interference from ambient light showed that the fabricated instrument was stable, accurate, capable of quantitative detection and insensitive to ambient light changes. In addition, this instrument is user-friendly since it could calculate and report the final amount of AFB1to the operator. Measurements of maize and peanuts showed that the instrument provided as accurate results as the professional equipment. With the low fabrication cost (about RMB 129 or USD 20), portability, and user-friendliness, this instrument presents attractive potential in the rapid detection of AFB1.Keywords:ELISA;colorimetric;food safety;mycotoxin;rapid detection
研究领域:
食品科学
·
分析化学
·
仪器仪表
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 33
·11563-11571
·
IF 7.059
Xiaoqin Zhang,Chongyuan Chen,Dongping Peng,Yizi Zhou,Jianle Zhuang,Xuejie Zhang,Bingfu Lei,Yingliang Liu,Chaofan Hu
Biogenic amines (BAs) from rotten food cause serious harm to sensitive people. Therefore, the development of a smart label that can intuitively monitor the degree of food decay in real-time is of great significance in food safety. Herein, we report the synthesis of pH-responsive carbon dots with red emission (R-CDs) by the solvothermal treatment of citric acid and urea. The fluorescence intensity of R-CDs gradually increased as the pH of the solution increased from 1 to 12. We studied the mechanisms of pH response and found that H-aggregation occurred when R-CDs were in acidic solution and caused quenching; when they were distributed in alkaline solution, the Fermi level shift caused reduced self-absorption and enhanced red fluorescence emission. The intense red emission of R-CDs is beneficial for the visual detection of alkaline organic amines and BAs derived from rotten shrimp to achieve food safety detection. Moreover, the R-CDs can be used as a promising pH-sensitive fluorescent ink, which has broad prospects and significance in anticounterfeiting and information encryption.
研究领域:
食品科学
·
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2023
·
IF 4.1
Lei Ding,Xuan Zhou,Xiaojie Liang,Yujin Dong,Cunbao Fang,Yueming Wu,Beixing Li,Wei Mu,Jin Lin,Yuekun Li
Pesticide residue has become the main technical barrier that restricts the export of Chinese wolfberry. Can we achieve high efficacy and low safety risk by balancing pesticide deposition on the leaves and fruits of Chinese wolfberry? In this research, the structural characteristics and wettability of leaves and fruits of Chinese wolfberry at different growth stages were studied. The adaxial and abaxial surfaces of leaves were hydrophobic, whereas the fruit surfaces were hydrophilic. Adding spray adjuvant could increase the retention of droplets on the leaf surfaces of Chinese wolfberry by 52.28–97.89% and reduce the retention on the fruit surfaces by 21.68–42.14%. A structural equation model analysis showed that the adhesion tension was the key factor affecting the retention of the solutions among various interface behaviors. When the concentrations of Silwet618, AEO-5, Gemini 31551, and 1227 were 2–5 times higher than their CMCs, the retention of pesticide solutions (pyraclostrobin and tylophorine) on Chinese wolfberry leaves significantly increased, and the control efficacies on aphids and powdery mildew also dramatically improved (65.90–105.15 and 41.18–133.06%, respectively). Meanwhile, the retention of pesticides on the fruit of Chinese wolfberry was reduced. This study provides new insights into increasing the utilization of pesticides in controlling pests and improving food safety.
研究领域:
植物保护
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2024
·
IF 2.1
Guo Jinxiu,Kong Tao,Zhang Huaijie,Ma Runtian,Ma Zijun,Hu Bing
The development of rapid and sensitive malachite green (MG) detection methods is particularly important for food safety supervision and ecological protection. Using fulvic acid (FA) as a stabilizer and NaBH 4 as a reducing agent, ellipsoidal gold nanoparticles (AuNPs) with the average particle size of 5–20 nm were successfully prepared. Based on the interaction between fulvic acid protected gold nanoparticles (FA-AuNPs) and MG, the color change of FA-AuNPs from ruby red to blue was observed, the corresponding absorbance ratio change of gold nanoparticles at 611 nm and 529 nm (A611/A529) was also studied. Through the optimization of experimental conditions, the linear relationship between A611/A529 with the concentration of MG was established. The detection limit and linear range of this method were 0.02 μM and 0.04–2 μM, respectively. This method was successfully applied to detect malachite green in three different water samples with the recovery of 97.4–110.8%. Graphical abstract
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Sai Zhang,Ruixi Yang,Yaping Wang,Qinqin Gao,Langhong Wang,Guisheng Zhou,Qinglin Sheng
In recent years, food-borne pathogen Pseudomonas cocovenenans have caused many food safety incidents. Therefore, the detection of toxoflavin produced by the bacteria has aroused widespread attention. In this study, two fluorescent sensors were prepared using nitrogen-doped carbon dots (NCDs) materials as precursors: (1) Luminol-functionalized NCDs were synthesized by NHS-EDC reaction, and L-NCDs fluorescence sensor was constructed for the detection of toxoflavins in actual food samples. The linear range of the L-NCDs fluorescence sensor is 0.1–50 μM, the detection limit is 0.05 μM and the excitation wavelength is 375 nm. (2) The NCDs-CMC fluorescent film sensor was constructed by incorporating NCDs into sodium carboxymethyl cellulose. It was excited at a wavelength of 385 nm for visual detection of toxoflavin. The film sensor has a linear range of 1–60 μM and a detection limit of 0.45 μM. The results showed that the method has good sensitivity and reliability. This study extends the application of NCDs-based fluorescence sensing to detect toxoflavin. It enables simple, rapid, low-cost sensitive detection and visual monitoring of toxoflavin. Additionally, it offers a fast and convenient new technology for preventing and controlling Pseudomonas cocovenenans contamination in food.
研究领域:
分析化学
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jintao Yan,Liangqiang Chen,Mengjing Teng,Mengdi Hao,Bin Feng,Fan Yang,Hao Shen,Shaoning Yu,Li Wang
Bacillus cereus is one of the most common foodborne pathogens and is widely distributed in foods, soil, water, plants and fodder. As an aerobic Gram-positive bacteria, B. cereus also plays an important role in liquor production, generating flavor enhancers and antibacterial substances. Therefore, a rapid and precise analysis strategy for this microbe is highly desired in food safety and sustainable food production. In this study, we propose an effective approach for B. cereus quantification using a dual recognition biosensor. B. cereus was specifically enriched with aptamer-functionalized magnetic beads, and then detected using the broad-spectrum fluorescent material, fragmented crystalline mannose-conjugated lectin-modified nano-MIL-53(Al)-NH 2 ( [email protected] ). The dual recognition method was applied for specific quantification of B. cereus in the range of 20–2 × 10 8 CFU/mL with a limit of detection of 4 CFU/mL within 60 min. Recoveries ranged from 93.8%−100.8% in milk, rice, chicken meat, eggs, and distilled yeast as real samples were also obtained.
研究领域:
微生物学
·
食品科学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Fenglan Zhou,Chunjie Zhou,Wenhua Zhang,Zsolt Zalán,Hui Shi,Jianquan Kan,Kewei Chen,Tian Cai
The detection of mycotoxins in Sichuan peppers is crucial for ensuring food safety. Hence, an efficient detection approach based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method followed by LC–MS/MS (liquid chromatography–tandem mass spectrometry) was established for the analysis of various mycotoxins in dried red and green Sichuan peppers. Samples were extracted with a solution containing 99 % acetonitrile and 1 % formic acid. A new adsorbent, namely, ENVI-Carb, and primary secondary amines were used as purifying agents. The proposed method demonstrated good linearity (R 2 ≥ 0.999), satisfactory recovery (77.3 %–109.2 %), and good repeatability (intraday and interday relative standard deviations ≤9.7 %) in dried red and green Sichuan peppers. Its limits of detection in red and green Sichuan peppers were 0.2–0.6 and 0.1–0.7 μg/kg, respectively. The established method can effectively eliminate complex matrix interference and provides an effective approach for the high-throughput detection of mycotoxins in various complex food matrices.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Yujie Wang,Peng Li,Yunhe Tian,Ziqin Xiong,Zhongyi Zheng,Zhenxie Yi,Hejun Ao,Qiming Wang,Juan Li
Cadmium (Cd) contamination in rice ( Oryza sativa ) is particularly problematic due to its high risk to human health. Investigating the hidden roles of seed endophytes of rice in influencing Cd accumulation is essential to comprehensively understand the effects of biotic and abiotic factors to food security. Here, the content of Cd in soils and rice (Huanghuazhan) seeds from 19 sites along the Yangtze River exhibited considerable differences. From a biotic perspective, we observed the dominant endophytic bacteria, Stenotrophomonas (7.25 %), contribute to Cd control of rice (below 0.2 mg kg −1 ). Partial Least Squares (PLS) analysis further suggested that Enterobacteriaceae (15.48 %), altitude and pH were found to be the strong variables that might reduce the Cd uptake of rice. In contrast, Cytophagaceae (0.58 %), latitude and mean annual air pressure had the opposite effect. In pot experiments, after respectively inoculating the isolated endophytic bacteria Stenotrophomonas T4 and Enterobacter R1, N1 (f_Enterobacteriaceae), the Cd contents in shoot decreased by 47.6 %, 21.9 % and 33.0 % compared to controls. The distribution of Cd resistant genes (e.g., czcABC, nccAB, cznA ) of Stenotrophomonas, Enterobacteriaceaea and Cytophagaceae further suggested their distinct manners in influencing the Cd uptake of rice. Overall, this study provides new insights into the food security threatened by globally widespread Cd pollution.
研究领域:
环境生物学
·
植物学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 9
·e15291
·
IF 2.19
Jingbo Zhang,Chaofan Ji,Jing Han,Yunsong Zhao,Xinping Lin,Huipeng Liang,Sufang Zhang
Toxic biogenic amines (BAs), especially histamine, in food is a major safety issue. Two autochthonous Lactobacillus plantarum strains were inoculated as starters to reduce BAs in Yucha, a kind of spontaneously fermented fish in China. Taxonomic analysis demonstrated the proportion of Lactobacillus genus in inoculated Yucha was higher than that in the spontaneously fermented Yucha, suggesting the effective colonization abilities of the starters. Higher abundance of Lactobacillus genus resulted in less BAs contents in the inoculated Yucha as compared to that in the spontaneously ones throughout the fermentation stage. At the end of fermentation, L. plantarum strains reduced total content of the six BAs by 67.5%–97.3%; specifically, they reduced the concentration of histamine by 85.4%–96.4%. The BAs-degradation ability in the model systems indicated that the two L. plantarum strains could not degrade BAs directly. Instead, starters reduced BAs accumulation by inhibition of the growth or biological activities of BAs-producing bacterial genera. Practical applications The presence of biogenic amines (BAs) in fish products poses a health risk for consumers. This work provided two promising starters to reduce BAs accumulation in Yucha and a foundation for better understanding the BAs metabolic features. These findings could contribute to the safe production of Yucha and other similar fermented fish products.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yanqing Xu,Zhaojiang Yin,Qian Long,Haotian Xie,Xinying Yan,Fusheng Liao,Hanfeng Cui,Hao Fan
This study reports the design and synthesis of a fluorescent probe TPA-ACL based on the triphenylamine (TPA) structure for the dual detection of hydrogen sulfide (H₂S) and water content. In the presence of H₂S, TPA-ACL exhibits a pronounced “turn-on” fluorescence response, demonstrating excellent selectivity and high sensitivity with a detection limit (LOD) of 2.02 nM. Furthermore, TPA-ACL enables real-time detection of H₂S using test strips, allowing for visual monitoring of H₂S gas generated during food spoilage, indicated by a color change from pale yellow to deep yellow. The probe was also successfully applied for the quantitative analysis of trace water content in food samples, with a detection limit (LOD) of 70.7 ppm (0.0071 vol%) in tetrahydrofuran (THF). This study provides a sensitive, efficient, and practical tool for detecting H 2 S and water content, demonstrating significant potential for applications in food safety monitoring.
研究领域:
分析化学
·
食品科学
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yingying Wang,Linping Ma,Shaogui Wu
Food safety concerns regarding Sudan Red, a carcinogenic dye, necessitate effective detection methods. This study introduces S, P co-doped blue-emitting carbon dots (BSP-CDs), synthesized via a one-pot hydrothermal method using asparagine as the carbon source. BSP-CDs exhibit excellent optical properties, environmental stability, and selective fluorescence quenching due to the inner filter effect. These properties were utilized to develop a sensitive and rapid “on-off” fluorescence sensing system for Sudan Red detection. The sensor demonstrated a linear detection range of 50–175 μM and a detection limit of 2.11 μM, with high accuracy and recovery rates in real-world water samples. This work offers a green, economical, and highly reproducible approach for Sudan Red monitoring, with significant implications for food safety and environmental protection.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）