Micro & Nano Letters
·
2018
·Vol. 13
·期 6
·868-871
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IF 0.841
Yan-meng Li,Li-wei Wang,Ruo-ping Li,Jun-he Han,Ming-ju Huang
Malachite green (C 23 H 25 ClN 2 ) is an illegal fungicide of aquatic products due to its potential carcinogenic, teratogenic, and mutagenic effects. It is typically challenging to detect C 23 H 25 ClN 2 in fish with conventional techniques. The study reported a simple and rapid method for detecting C 23 H 25 ClN 2 on fish surface with surface-enhanced Raman scattering (SERS). Using the seed growth method with trometamol as the modifier, 110 nm in-diameter gold nanoparticles on thin glass as SERS substrate were prepared, which exhibited high SERS activity, reproducibility, stability, and flexibility in SERS measurement. The fish samples required no pretreatment and the entire measurement took <5 min. Quantitative analyses were also conducted. Excellent linear relationships ( R 2 = 0.9397–0.9977) were obtained between the concentrations and the peak intensities of C 23 H 25 ClN 2 at some characteristic peaks with a wide concentrations range (0.5–10 μM). The detection limit can reach 0.5 μM. This method provided an easy and fast way to detect C 23 H 25 ClN 2 on fish surface for food safety.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Sai Zhang,Ruixi Yang,Yaping Wang,Qinqin Gao,Langhong Wang,Guisheng Zhou,Qinglin Sheng
In recent years, food-borne pathogen Pseudomonas cocovenenans have caused many food safety incidents. Therefore, the detection of toxoflavin produced by the bacteria has aroused widespread attention. In this study, two fluorescent sensors were prepared using nitrogen-doped carbon dots (NCDs) materials as precursors: (1) Luminol-functionalized NCDs were synthesized by NHS-EDC reaction, and L-NCDs fluorescence sensor was constructed for the detection of toxoflavins in actual food samples. The linear range of the L-NCDs fluorescence sensor is 0.1–50 μM, the detection limit is 0.05 μM and the excitation wavelength is 375 nm. (2) The NCDs-CMC fluorescent film sensor was constructed by incorporating NCDs into sodium carboxymethyl cellulose. It was excited at a wavelength of 385 nm for visual detection of toxoflavin. The film sensor has a linear range of 1–60 μM and a detection limit of 0.45 μM. The results showed that the method has good sensitivity and reliability. This study extends the application of NCDs-based fluorescence sensing to detect toxoflavin. It enables simple, rapid, low-cost sensitive detection and visual monitoring of toxoflavin. Additionally, it offers a fast and convenient new technology for preventing and controlling Pseudomonas cocovenenans contamination in food.
研究领域:
分析化学
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食品科学
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微生物学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2023
·
IF 6.8
Yujie Wang,Peng Li,Yunhe Tian,Ziqin Xiong,Zhongyi Zheng,Zhenxie Yi,Hejun Ao,Qiming Wang,Juan Li
Cadmium (Cd) contamination in rice ( Oryza sativa ) is particularly problematic due to its high risk to human health. Investigating the hidden roles of seed endophytes of rice in influencing Cd accumulation is essential to comprehensively understand the effects of biotic and abiotic factors to food security. Here, the content of Cd in soils and rice (Huanghuazhan) seeds from 19 sites along the Yangtze River exhibited considerable differences. From a biotic perspective, we observed the dominant endophytic bacteria, Stenotrophomonas (7.25 %), contribute to Cd control of rice (below 0.2 mg kg −1 ). Partial Least Squares (PLS) analysis further suggested that Enterobacteriaceae (15.48 %), altitude and pH were found to be the strong variables that might reduce the Cd uptake of rice. In contrast, Cytophagaceae (0.58 %), latitude and mean annual air pressure had the opposite effect. In pot experiments, after respectively inoculating the isolated endophytic bacteria Stenotrophomonas T4 and Enterobacter R1, N1 (f_Enterobacteriaceae), the Cd contents in shoot decreased by 47.6 %, 21.9 % and 33.0 % compared to controls. The distribution of Cd resistant genes (e.g., czcABC, nccAB, cznA ) of Stenotrophomonas, Enterobacteriaceaea and Cytophagaceae further suggested their distinct manners in influencing the Cd uptake of rice. Overall, this study provides new insights into the food security threatened by globally widespread Cd pollution.
研究领域:
环境生物学
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植物学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 9
·e15291
·
IF 2.19
Jingbo Zhang,Chaofan Ji,Jing Han,Yunsong Zhao,Xinping Lin,Huipeng Liang,Sufang Zhang
Toxic biogenic amines (BAs), especially histamine, in food is a major safety issue. Two autochthonous Lactobacillus plantarum strains were inoculated as starters to reduce BAs in Yucha, a kind of spontaneously fermented fish in China. Taxonomic analysis demonstrated the proportion of Lactobacillus genus in inoculated Yucha was higher than that in the spontaneously fermented Yucha, suggesting the effective colonization abilities of the starters. Higher abundance of Lactobacillus genus resulted in less BAs contents in the inoculated Yucha as compared to that in the spontaneously ones throughout the fermentation stage. At the end of fermentation, L. plantarum strains reduced total content of the six BAs by 67.5%–97.3%; specifically, they reduced the concentration of histamine by 85.4%–96.4%. The BAs-degradation ability in the model systems indicated that the two L. plantarum strains could not degrade BAs directly. Instead, starters reduced BAs accumulation by inhibition of the growth or biological activities of BAs-producing bacterial genera. Practical applications The presence of biogenic amines (BAs) in fish products poses a health risk for consumers. This work provided two promising starters to reduce BAs accumulation in Yucha and a foundation for better understanding the BAs metabolic features. These findings could contribute to the safe production of Yucha and other similar fermented fish products.
研究领域:
食品科学
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微生物学
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生物技术
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Yingying Wang,Linping Ma,Shaogui Wu
Food safety concerns regarding Sudan Red, a carcinogenic dye, necessitate effective detection methods. This study introduces S, P co-doped blue-emitting carbon dots (BSP-CDs), synthesized via a one-pot hydrothermal method using asparagine as the carbon source. BSP-CDs exhibit excellent optical properties, environmental stability, and selective fluorescence quenching due to the inner filter effect. These properties were utilized to develop a sensitive and rapid “on-off” fluorescence sensing system for Sudan Red detection. The sensor demonstrated a linear detection range of 50–175 μM and a detection limit of 2.11 μM, with high accuracy and recovery rates in real-world water samples. This work offers a green, economical, and highly reproducible approach for Sudan Red monitoring, with significant implications for food safety and environmental protection.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yanqing Xu,Zhaojiang Yin,Qian Long,Haotian Xie,Xinying Yan,Fusheng Liao,Hanfeng Cui,Hao Fan
This study reports the design and synthesis of a fluorescent probe TPA-ACL based on the triphenylamine (TPA) structure for the dual detection of hydrogen sulfide (H₂S) and water content. In the presence of H₂S, TPA-ACL exhibits a pronounced “turn-on” fluorescence response, demonstrating excellent selectivity and high sensitivity with a detection limit (LOD) of 2.02 nM. Furthermore, TPA-ACL enables real-time detection of H₂S using test strips, allowing for visual monitoring of H₂S gas generated during food spoilage, indicated by a color change from pale yellow to deep yellow. The probe was also successfully applied for the quantitative analysis of trace water content in food samples, with a detection limit (LOD) of 70.7 ppm (0.0071 vol%) in tetrahydrofuran (THF). This study provides a sensitive, efficient, and practical tool for detecting H 2 S and water content, demonstrating significant potential for applications in food safety monitoring.
研究领域:
分析化学
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食品科学
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环境监测
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jie Liu,Jiang Zhou,Qiang Xi,Shengyuan Yang,Wenfang Du,Fubing Xiao
Bisphenol A (BPA), an endocrine disruptor , is widely used in food packaging materials , including drink containers. Sensitive detection of BPA is crucial to food safety. Herein, we have developed a novel optical-driven hydrogel film sensor for sensitive BPA detection based on the displacement of spiropyran (SP) from β -cyclodextrin ( β -CD) cavity by BPA followed by the photochromism of the released SP. The released SP converts to the ring-opened merocyanine form which shows an enhanced red fluorescence in the dark. The sensor demonstrates a linear detection range from 0.1 to 20 μg mL −1 with a limit of detection at 0.027 μg mL −1 and a limit of quantification at 0.089 μg mL −1 . Notably, the proposed β -CD/SP hydrogel can be reused due to the reversible isomerization of SP and the reversible host–guest interaction. This sensor also shows good performance for BPA determination in real samples, indicating its great potential for food safety monitoring.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Fenglan Zhou,Chunjie Zhou,Wenhua Zhang,Zsolt Zalán,Hui Shi,Jianquan Kan,Kewei Chen,Tian Cai
The detection of mycotoxins in Sichuan peppers is crucial for ensuring food safety. Hence, an efficient detection approach based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method followed by LC–MS/MS (liquid chromatography–tandem mass spectrometry) was established for the analysis of various mycotoxins in dried red and green Sichuan peppers. Samples were extracted with a solution containing 99 % acetonitrile and 1 % formic acid. A new adsorbent, namely, ENVI-Carb, and primary secondary amines were used as purifying agents. The proposed method demonstrated good linearity (R 2 ≥ 0.999), satisfactory recovery (77.3 %–109.2 %), and good repeatability (intraday and interday relative standard deviations ≤9.7 %) in dried red and green Sichuan peppers. Its limits of detection in red and green Sichuan peppers were 0.2–0.6 and 0.1–0.7 μg/kg, respectively. The established method can effectively eliminate complex matrix interference and provides an effective approach for the high-throughput detection of mycotoxins in various complex food matrices.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Siying Che,Yiwan Zhuge,Xinxiang Shao,Xiutan Peng,Haiyan Fu,Yuanbin She
Food fraud caused by the violation of glyphosate use in tea is frequently exposed, posing a potential health risk to consumers and undermining trust in food safety. In the work, an ionic fluorescent probe “[P 66614 ] [4HQCA]-Cu 2+ ( PHQCA-Cu 2+ )” was constructed using Cu 2+ and ionic liquids coordination through a competitive coordination strategy to detect glyphosate. This probe exhibited a prominent “turn-on” fluorescence response in glyphosate detection. PHQCA-Cu 2+ was destroyed by glyphosate with its strong coordination capability, and a new complex re-formed simultaneously between glyphosate and the Cu 2+ in it, where Cu 2+ served as an “invisible indicator” influencing fluorescence changes. Remarkably, PHQCA-Cu 2+ formed rapidly within 5 s, demonstrated exceptional sensitivity and selectivity, and satisfactory detection performance on paper strips impregnated with PHQCA-Cu 2+ . Importantly, PHQCA-Cu 2+ showed excellent recoveries in various green tea, which offered a viable method for identifying contaminated products from the supply chain quickly to enhance overall food safety surveillance.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Ying Hu,Lingmin Lu,Shiman Guo,Xiaolan Wu,Jiayi Zhang,Chunsong Zhou,Haiyan Fu,Yuanbin She
A reversible fluorescent probe Pyr-1 was developed by integrating pyrene and oxime functional groups and applied for visual detection of Cysteine (Cys). By featuring three binding sites, Pyr-1 exhibited selective fluorescence quenching response towards Fe 3+ through the formation of 1:1 coordination complex. The ensemble Pyr-1-Fe 3+ was further applied to detect Cys. Among the various test species, Cys induced unique fluorescence enhancement through the decomplexation process. The detection of Cys exhibited a fluorescence “turn-off-on” phenomenon within 50 s and reversible cycle performed up to at least 32 times. The detection limit was calculated as 0.446 µM. Due to the excellent stability in wide pH range (2−11), Pyr-1-Fe 3+ was successfully applied in detection of Cys in diverse food samples. In practical applications, an obvious fluorescent color difference was observed on paper-based device modified by Pyr-1-Fe 3+ , which was convenient and could be used for on-site evaluation of Cys in food samples.
研究领域:
分析化学
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食品科学
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生物化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Caihong Zhou,Jingjing Fan,Liangyuan Wang,Baoxue Gao,Chunhui Wei,Long Ma,Lijuan Yin
Norovirus (NoV) is a leading cause of nonbacterial acute gastroenteritis, primarily transmitted through contaminated food and water. Rapid, sensitive, and on-site detection is crucial for controlling NoV outbreaks and ensuring food safety. In this study, we developed a portable, one-tube CRISPR/Cas12a-driven colorimetric biosensor for the rapid and sensitive detection of NoV RNA without the need for pre-amplification. The biosensor integrates magnetic nanoprobes with gold nanoparticle (AuNP) bioprobes, connected via single-stranded DNA (ssDNA) linkers. Upon the specific recognition of NoV cDNA, the Cas12a protein’s trans -cleavage activity is activated, cleaving the ssDNA linkers and releasing AuNP bioprobes from the magnetic nanoprobes. This release results in a visually detectable red color change and enhanced UV–visible absorbance in the solution following magnetic separation. These changes can be easily detected using portable devices such as microplate readers or smartphones. This method achieves a detection limit of 10 copies/µL within 60 min, with no cross-reactivity from other viruses or bacteria. Validation using oyster samples showed perfect concordance with qPCR results. Designed for resource-limited settings, our biosensor can be adapted for detecting various pathogens by altering the target nucleic acids and crRNA sequences. This advancement offers significant potential for enhancing rapid diagnostics and outbreak management.
研究领域:
分子生物学
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食品科学
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诊断技术
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Junling He,Yong Tang,Chiliang Lin,Shaoyun Peng,Stig Pedersen-Bjergaard,Frederik André Hansen,Chen Zhou
Effective monitoring of veterinary drug residues in food is essential for legislation compliance and food safety, yet remains challenging due to low concentrations and complex matrices. This study introduced a miniaturized 96-well electromembrane extraction (EME) technique for pre-concentration and isolation 80 prohibited/restricted veterinary drugs from honey samples. Three liquid membranes were developed and characterized: V 1 (“V” for veterinary), a mixture of 2-undecanone and 0.5 % 2-nitro-p-cymene, was optimal for extraction of quinolones, tetracyclines, and β -agonists. V2, a mixture of 2-nitrophenyl octyl ether and a deep eutectic solution (formed upon mixing 6-methylcoumarin with thymol in a 1:1 w /w ratio) in a 1:1 v /v ratio, with the addition of 0.5 % di(2-ethylhexyl) phosphate (DEHP), was suitable for macrolides and sulfonamides extraction. V3, a deep eutectic solvent formed upon 1-decanoic acid and camphor in a 1:1 w /w ratio with 1 % DEHP, was suitable for aminoglycosides and β -lactams. Operational parameters were fine-tuned to achieve optimal extraction efficiencies, with recoveries ranging from 20.1–117.5 %, 20.3–91.9 %, and 20.1–118.8 % for V1, V2, and V3, respectively. High precision was observed for compounds with recoveries over 40 % (intra-day: 0.4–27.7 %; inter-day: 2.6–33.0 %), and matrix effects were generally within 80–120 %. This work established a theoretical framework for EME in food analysis, highlighting its potential as a powerful sample pretreatment method for the detection of veterinary drug residues. The results demonstrated the feasibility and advantages of using EME for ensuring food safety and regulatory compliance.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）