Analytical Methods
·
2023
·Vol. 16
·期 2
·276-283
·
IF 3.1
Ting Xu,Qing Fu,Zeng Qingru,Zhaojie Wang,Xin Liu,Shensheng Xiao,Xiaoming Jiang,Yuepeng Lu,Zhiyong Gong,Yongning Wu,Min Fang
A rapid fluorescence detection method was established for 3-monochloropropane-1,2-diol (3-MCPD). The detection system works based on the fluorescence quenching of pyrocatechol–polyethyleneimine (PCh–PEI) polymer by 3-MCPD. The fluorescence quenching ability of 3-MCPD for PCh–PEI polymer was measured at different pH and temperatures. Indeed, in the presence of 3-MCPD, the fluorescence intensity of PCh–PEI polymer solution was quenched best at 100 °C and pH 8.5. Also, the effect of different concentrations of 3-MCPD on the optical properties of the PCh–PEI polymer was examined. Under optimal experimental conditions, fluorescence detection was linear in a range of 0.08–2.0 mg per L 3-MCPD, with a calculated detection limit of 0.06 mg L−1 and a correlation coefficient of 0.9974. Concisely, the reported method has good sensitivity and can be used for the rapid detection of 3-MCPD contamination in food products.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 11
·4709-4718
·
IF 4.813
Fan Qiuxia,Yuan Yahong,Jia Hang,Zeng Xuejun,Wang Zhouli,Hu Zhongqiu,Gao Zhenpeng,Yue Tianli
Abstract Shigella flexneri ( Sh. flexneri ), a common foodborne pathogen, has become one of the main threats to food safety and human health due to its high pathogenicity and persistent infection. The objective of this study was to explore the antimicrobial and anti-biofilm activities and the possible mechanism of thymoquinone (TQ) against Sh. flexneri . The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of TQ against Sh. flexneri were 0.4 and 0.5 mg/mL, respectively. TQ showed bactericidal activity against Sh. flexneri in culture medium and milk system. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) observations demonstrated that TQ could induce abnormal cell morphology and destroy cell membrane. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis suggested that TQ could inhibit protein synthesis in Sh. flexneri . Also, at sub-inhibitory concentrations (SICs), TQ exhibited an inhibitory effect on Sh. flexneri biofilm formation, which was confirmed by crystal violet quantitative analysis and SEM observation. Real-time quantitative PCR (RT-qPCR) analyses revealed that TQ downregulated the expression of genes involved in Sh. flexneri biofilm formation. Thus, TQ has potential as a natural antimicrobial and anti-biofilm agent to address the contamination and infection caused by Sh. flexneri . Key points • Antimicrobial and anti-biofilm activity of TQ on Shigella flexneri were investigated.
• TQ inhibited biofilm formation by Shigella flexneri.
• TQ provided a new strategy for Shigella flexneri control.
研究领域:
微生物学
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食品安全
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Hong Dai,Lingshan Gong,Guifang Xu,Shupei Zhang,Shuanyan Lu,Yuwei Jiang,Yanyu Lin,Longhua Guo,Guonan Chen
A simple and sensitive electrochemical sensor which was constructed with alkoxy silane and carbon nanohorns was first utilized to detect some food borne contaminants, including malachite green, sudans, triclosan, bisphenol A. Due to the large surface area, good conductivity, admirable electrochemical activity of carbon nanohorns and excellent immobility of alkoxy silane, the synergistic effect root from the easily controlled composition of organic–inorganic hybrid film was achieved. The present work demonstrated this functional film brings advantageous features such as stable and strong adherence to electrode matrix and excellent electrochemical catalysis, to electrochemical sensor to achieve advanced application. By choosing triclosan and malachite green as object, their kinetic parameters on this modified electrode were calculated and the relative quantitive protocols were established. Compared with previous reports, the designed sensor presented lower detection limit, higher sensitivity and more broad linear range. This electrochemical platform offered a useful tool for the on-site determination of food born contamination.
研究领域:
分析化学
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食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENT INTERNATIONAL
·
2021
·
IF 9.621
Kai Cui,Xiaohu Wu,Dongmei Wei,Ying Zhang,Junli Cao,Jun Xu,Fengshou Dong,Xingang Liu,Yongquan Zheng
Neonicotinoid pesticide residues are ubiquitous in various foodstuffs and may adversely affect human health. We performed a nationwide survey of neonicotinoid residues in foodstuffs collected from Chinese markets and evaluated the risks of chronic and acute exposure in 1–6-year-old children and the general population. Among the 3406 samples of 13 commodities, 62.21% contained neonicotinoids with concentrations ranging from 0.1 to 1471.43 μg/kg, and 37.58% were simultaneously contaminated with 2–7 neonicotinoids. Acetamiprid, thiamethoxam, and imidacloprid were the top three detected neonicotinoids (22.14–34.32% of samples). Chronic and acute cumulative risk assessment using the relative potency factor method revealed that exposure to neonicotinoids was within established safety limits (below 1); however, the acute risk was much greater than the chronic risk (chronic hazard index range, 1.40 × 10 −6 –2.33 × 10 −3 ; acute hazard index range, 1.75 × 10 −6 –0.15). A relatively greater acute cumulative risk was found for children with respect to consumption of grapes, mandarins, and cowpeas (acute hazard index range, 0.11–0.15). Despite the low health risk, the potential health hazards of neonicotinoids should be continuously assessed, given their ubiquity and cumulative effects.
研究领域:
食品安全
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毒理学
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环境健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qiyue Zhao,Hong-Man Hou,Gong-Liang Zhang,Hongshun Hao,Bei-wei Zhu,Jingran Bi
Heterocyclic aromatic amines (HAAs) are the main carcinogens produced during thermal processing of protein-rich foods. In this paper, a composite aerogel (TOCNF Ca ) with a stabilized dual-network structure was prepared via a template for the in-situ synthesis of UiO-66 on cellulose for the adsorption of HAAs in food. The dual-network structure of TOCNF Ca provides the composite aerogel with excellent wet strength , maintaining excellent compressive properties. With the in-situ grown UiO-66 content up to 71.89 wt%, the hierarchical porosity endowed TOCNF Ca @UiO-66 with the ability to rapidly adsorb HAAs molecules with high capacity (1.44–5.82 μmol/g). Based on excellent thermal stability , adsorption capacity and anti-interference, TOCNF Ca @UiO-66 achieved satisfactory recoveries of HAAs in the boiled marinade, which is faster and more economical than the conventional SPE method. Moreover, TOCNF Ca @UiO-66 could maintain 84.55 % of the initial adsorption capacity after 5 times of reuse.
研究领域:
食品科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Ling Yao,Lei Ouyang,Jiping Lv,Pei Dai,Lihua Zhu
There is an increasing demand for rapid and sensitive detection of hydrophobic contaminants in foods. A rapid and sensitive surface enhanced Raman scattering (SERS) detection method was developed for this purpose, based on the surface modification of nano-Ag aggregates, which had rich hot spots and controllable hydrophobicity. Cationic surfactants with different carbon chain lengths were employed to modify the aggregation of Ag nanoparticles. The adsorption of cationic surfactants on nano-Ag aggregates increased the hydrophobicity of the surface of the nano-Ag aggregates as the SERS substrate. By changing the carbon chain length of the surfactant, the hydrophobicity of the SERS substrate was able to be tuned to match with the hydrophobicity of the contaminants to be detected. Different hydrophobic contaminants such as Auramine O, Sudan I and Sudan III could be quantitatively detected, with a detection limit as low as 6.1 × 10 −8 , 7.5 × 10 −9 , 2.1 × 10 −9 mol L −1 , respectively. Compared with the traditional inorganic salt aggregation method which was unable to meet the requirements of direct quantitative detection in the sample matrices, the developed method was confirmed to have the advantages of low detection limit, good reproducibility (relative standard deviation (RSD) is less 7.6%), short operation time (about 1 min), and no need of any complicate pretreatment for sample preparation.
研究领域:
分析化学
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食品安全
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 9
·1993-2001
·
IF 2.557
Tianfu Wei,Gongke Li,Zhuomin Zhang
Semicarbazide, a toxic food contaminant, widely exists in food products and it originates from the thermal degradation of a food additive of azodicarbonamide or a metabolite of nitrofurazone abused in meat specimens. Many previous methods for semicarbazide determination usually required expensive instruments, difficult-to-prepare monoclonal antibodies, and a long operation time. In this study, a high-performance liquid chromatography method was developed for the rapid determination of trace semicarbazide coupling with a nucleophilic substitution reaction firstly using 4-nitrobenzoyl chloride as derivatization reagent. The derivatization reaction was mild at room temperature for 1 min in neutral solution. Then, semicarbazide derivative was separated and quantified by high-performance liquid chromatography with ultraviolet detection under optimal separation conditions at λ max = 261 nm. The proposed method offered the detection limit of 1.8 μg/L and was successfully applied for the rapid determination of trace semicarbazide in flour products. Semicarbazide in positive real samples could be actually found and quantified in the range of 0.47−7.53 mg/kg. The recoveries were 76.6−119% with relative standard deviations of 0.5–9.1% ( n = 3). This developed method was rapid, reliable, and convenient for the determination of trace semicarbazide in food.
研究领域:
分析化学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Mei Sun,Ke Chen,Hanmei Hu,Ping Chen,Mingdi Yang,Haiou Zhou,Han Xuan
Electrically modulated surface-enhanced Raman scattering (E-SERS) enhances Raman signals by adjusting the plasmon resonance of noble metal nanostructures with an electric field. However, traditional E-SERS substrates require complex electrochemical setups, limiting portability. Piezoelectric materials, which generate surface charges under external forces, present a promising alternative. This study develops a novel self-powered E-SERS substrate by embedding piezoelectric material 0.5Ba(Zr 0.2 Ti 0.8 )O 3 -0.5(Ba 0.7 Ca 0.3 )TiO 3 (BZT-BCT) in polyvinylidene difluoride-hexafluoropropylene (PVDF-HFP) matrix combined with silver nanoparticles. Experiments with nanogenerator piezoelectricity reveal that pressing the substrate with a finger generates current and voltage, providing an electric field for SERS enhancement. Gradual addition of weights on the substrate significantly boosts Raman signals of molecules like p-aminothiophenol (PATP), crystal violet (CV), thiram, and methyl bromide (MB). The synergy of piezoelectric effect and localized plasmon resonance achieves high sensitivity, reproducibility, and detection limits down to 10 −9 M, with an enhancement factor of 10 9 . Moreover, the sensor effectively detects trace MB veterinary drug residues in fish meat and thiram pesticide residues on apple skin, introducing a new self-powered E-SERS platform for sensitive food contaminant detection.
研究领域:
材料科学
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分析化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Hongyan Yang,Lan Wang,Fei Pan,Libo Yuan,Heng Du,Pei Zhang,Kui Lu
Food contamination caused by bacteria is an important challenge in the food industry. In this study, the peptide derivative C12−CL5 was produced by attaching a 12-carbon saturated fatty acid to the N-terminus of the anti-Gram-negative bacterial peptide CL5. The amphiphilic lipopeptide C12−CL5 self-assembled into core-shell nanostructures at concentrations above its critical aggregation concentration. It was found that the self-assembling C12−CL5 showed potent activity against both Gram-negative and Gram-positive bacteria, with minimum inhibitory concentrations of 4–32 μM. Moreover, C12−CL5 exhibited good thermal stability, excellent salt tolerance, as well as strong and rapid killing kinetics. Studies on the antimicrobial mechanism of C12−CL5 indicated that it could disrupt cell membranes, leading to leakage of cellular contents and ultimately to bacterial death. This study revealed that the self-assembly of antimicrobial peptides improved their antimicrobial activity and spectrum. It provides the insights and basis for the development of potent and broad-spectrum antimicrobial peptides for use, e.g., in the food industry.
研究领域:
微生物学
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食品安全
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Nan Yang,Ting-Ting You,Yu-Kun Gao,Chen-Meng Zhang,Peng-Gang Yin
Surface enhanced Raman scattering (SERS) has been widely used in detection of food safety due to the nondestructive examination property. Here, we reported a flexible SERS film based on a polymer-immobilized gold nanorod polymer metafilm. Polystyrene–polyisoprene–polystyrene (SIS), a transparent and flexible, along with having excellent elasticity, polymer, was chosen as the main support of gold nanorods. A simple phase transfer progress was adopted to mix the gold nanorods with the polymer, which can further be used in most water-insoluble polymers. The SERS film performed satisfactorily while being tested in a series of standard Raman probes, like crystal violet (CV) and malachite green (MG). Moreover, the excellent reproducibility and elastic properties make the film a promising substrate in practical detection. Hence, the MG detection on the fish surface and trace thiram detection on orange pericarp were inspected with detection results of 1 × 10–10 and 1 × 10–6 M, which were below the demand of the National standard of China, exactly matching the realistic application requirements.
研究领域:
材料科学
·
食品安全
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Xin Meng,Yuanyuan Yue,Mengyang Huang,Zhangqun Duan,Kang Liu,Lina Wu
Background Lead ion (Pb 2+ ) is a toxic heavy metal that poses severe threats to food safety. Traditional methods like inductively coupled plasma mass spectrometry (ICP-MS) and atomic absorption spectrometry (AAS) rely on bulky instrumentation, which are limited by high costs, complex sample preparation requirements, and inability to meet the demands for rapid on-site testing. Nanozyme-based biosensors have emerged as promising alternatives, yet single-mode sensors often suffer from matrix interference in complex food samples. Therefore, developing a rapid and reliable on-site method is critical for Pb 2+ detection. Results To address this challenge, a CRISPR/Cas12a-driven dual-mode biosensor integrating Pt/CeO 2 nanozyme-mediated peroxidase activity with GR-5 DNAzyme recognition was developed. The biosensor's design capitalizes on GR-5 DNAzyme for Pb 2+ -specific recognition, triggering CRISPR/Cas12a-mediated cleavage of electrochemical/colorimetric signal probe SH-ssDNA-Pt/CeO 2 to generate dual signals. This innovative platform synergizes electrochemical precision and colorimetric simplicity for Pb 2+ detection in complex food matrices. The biosensor achieved an ultra-sensitive electrochemical response (linear range: 0.002–200 nM; limit of detection: 0.14 pM) alongside a robust colorimetric readout (linear range: 0.5–2000 nM; limit of detection: 0.47 nM), representing significant sensitivity improvements over conventional single-mode sensors. Crucially, the intrinsic cross-verification mechanism between orthogonal signal modalities minimized false positives while ensuring >90.5 % recovery in spiked corn, edible oil, beef and red wine samples with RSD <5 %. These results highlight the potential of the dual-mode sensor as a practical, field deployable sensing platform for the detection of Pb 2+ . Significance This work innovatively integrates the specificity of CRISPR with the catalytic properties of nanozymes into a self-validating electrochemical/colorimetric dual-mode system. The detection method not only establishes a robust platform for highly sensitive, reliable and visualized detection of Pb 2+ in complex food matrices, but also pioneers a new paradigm for multi-modal biosensor design.
研究领域:
分析化学
·
食品安全
·
生物传感器
·
环境毒理学
·
分子诊断
·
纳米生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Benchao Su,Zhong Zhang,Zhichang Sun,Zongwen Tang,Xiaoxia Xie,Qi Chen,Hongmei Cao,Xi Yu,Yang Xu,Xing Liu,Bruce D. Hammock
Ochratoxin A (OTA) contamination in food is a serious threat to public health. There is an urgent need for development of rapid and sensitive methods for OTA detection, to minimize consumer exposure to OTA. In this study, we constructed two OTA-specific fluonanobodies (FluoNbs), with a nanobody fused at the carboxyl-terminal (SGFP-Nb) or the amino-terminal (Nb-SGFP) of superfolder green fluorescence protein. SGFP-Nb, which displayed better fluorescence performance, was selected as the tracer for OTA, to develop a FluoNb-based nanosensor (FN-Nanosens) via the fluorescence resonance energy transfer, where the SGFP-Nb served as the donor and the chemical conjugates of OTA-quantum dots served as the acceptor. After optimization, FN-Nanosens showed a limit of detection of 5 pg/mL, with a linear detection range of 5–5000 pg/mL. FN-Nanosens was found to be highly selective for OTA and showed good accuracy and repeatability in recovery experiments using cereals with various complex matrix environments. Moreover, the contents of OTA in real samples measured using FN-Nanosens correlated well with those from the liquid chromatography with tandem mass spectrometry . Therefore, this work illustrated that the FluoNb is an ideal immunosensing tool and that FN-Nanosens is reliable for rapid detection of OTA in cereals with ultrahigh sensitivity.
研究领域:
食品安全
·
生物传感
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）