SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yujuan Qin,Pengcheng Huang,Fang-Ying Wu
With food safety problems abounding, it is essential to explore some efficient and effective methods for monitoring food quality. To this end, we have developed a histamine-responsive carbon dots (CDs)-based sensing system for visual monitoring of food spoilage. In this system, the aldehyde group on the surface of CDs serves as the recognition sites to combine with the amino group on histamine to form a Schiff base that effectively enhances the fluorescence. On the other hand, 1-aminoanthraquinone (ANQ) was introduced as a reference signal to endow the fluorescent probe with self-calibrating capacity. As the histamine concentration increases, linear ratiometric response was obtained accompanied with a distinguishable fluorescence color change from red to blue. Besides, test strips-based smartphone visual assays were successfully used to evaluate and monitor the histamine levels in fish samples. Our proposed sensing strategy has been demonstrated to be efficient and robust along with great potential for real-time visual monitoring of food spoilage.
研究领域:
食品安全
·
生物传感
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Sensors & Diagnostics
·
2025
·
IF 4.1
Teody Gumabat,Jeanne Phyre Lagare Oracion,Jolina Fedelis,Ethel Keleste,Rey Capangpangan,Noel Lito Sayson,Gerard Dumancas,Arnold Alguno,Felmer Latayada
Spermine, a key biogenic amine indicating food freshness, is typically detected using costly and time-consuming chromatographic methods. This study introduces a more efficient, eco-friendly alternative—a label-free colorimetric sensing platform using gold nanoparticles (AuNPs) functionalized with 11-mercaptoundecanoic acid and capped with an N-hydroxysuccinimide (NHS)-ester. Transmission electron microscopy revealed monodisperse, spherical AuNPs (13 nm), with an increase in size upon functionalization. Fourier transform infrared spectra confirmed successful functionalization. The hydrodynamic size of the AuNPs increased from 17.26 nm to 2167 nm, and the zeta potential shifted from −48.86 mV to −35.59 mV. The platform takes advantage of the selective interaction between spermine and NHS-ester-functionalized AuNPs, inducing nanoparticle aggregation, as shown by a red shift in surface plasmon resonance (SPR). UV-vis spectroscopy demonstrated a robust linear correlation (R2 = 0.958) between spermine concentration (1.0–4.0 μM) and nanoparticle aggregation index, with a limit of detection (LOD) of 0.77 μM. The sensor also exhibited high reproducibility in pork extract matrices (coefficient of variation <5%) and selectivity for spermine amid various interfering analytes. Its eco-friendly design and rapid response time position it as a viable tool for real-time spermine monitoring in food spoilage, offering comparable performance metrics to traditional chromatographic techniques while addressing sustainability concerns.
研究领域:
生物化学
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食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Mengyuan Qin,Shuo Han,Miaomiao Chen,Pengju Li,Yuqi Wang,Wenfang Niu,Chao Gao,Hailei Wang,Yi Li
Hafnia paralvei , a Gram-negative foodborne pathogen, is found ubiquitously in various aquatic animals and seafoods, which can form biofilm as a dominant virulence factor that contributes to its pathogenesis. However, the biofilm formation mechanism of H. paralvei and its effect on food spoilage has not been fully characterized. Here we show that biofilm formation, is regulated by c-di-GMP which mediated by bcsB , can increase the spoilage ability of H. paralvei . We found that GTP was added exogenously to enhance the synthesis of c-di-GMP, which further promoted biofilm formation. The gene dgcC , one of 11 genes encoding GGDEF domain-containing proteins in H. paralvei , was significantly upregulated with GTP as substrate. The upregulation of dgcC contributes to a significant increase of c-di-GMP and the formation of biofilm. In addition, the overexpression of dgcC induced upregulation of bcsB , a reported effector protein encoding gene, which was further demonstrated that overexpression of bcsB can encourage the synthesis of bacterial cellulose and biofilm formation. The effect of biofilm formation induced by c-di-GMP on spoilage of Yellow River carp ( Cyprinus carpio ) was evaluated by sensory evaluation , the total viable count , and the total volatile basic nitrogen, which showed that biofilm formation can significantly increase the spoilage ability of H. paralvei on C. carpio . Our findings provide the regulation of c-di-GMP on expression of bcsB , that can contribute to biofilm formation and spoilage ability of H. paralvei , which is favor to understanding the pathogenesis of Hafnia paralvei and its role in food spoilage.
研究领域:
微生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Kai Xu,Lewei Gao,Yueyin Liang,Zhiyuan Meng,Shuai Gong,Zhonglong Wang,Shifa Wang
Hydrogen sulfide (H 2 S) has multiple contributions to living organisms and is also linked to the freshness of most high-protein foodstuffs. Therefore, developing a highly sensitive technology for on-site detection of H 2 S is promising and crucial. In this paper, a novel ratiometric near-infrared fluorescent probe 1,1,3-trimethyl-2-(4-(5,5,7-trimethyl-4,5,6,7-tetrahydro-3 H -4,6-methanobenzo[ d ]imidazol-2-yl)styryl)− 1 H -benzo[ e ]indol-3-ium iodide ( TIBI ) was designed and synthesized from α -pinene derivative 4,6,6-trimethylbicyclo[3.1.1]heptane-2,3-dione for the specific detection of H 2 S. TIBI displayed several excellent merits during the detection of H 2 S including low detection limit (19.43 nM), short response time (50 s), and wide pH detection range (5 9). The emission wavelength of TIBI blue-shifted from 690 nm to 460 nm accompanying with the fluorescence color changed from red to blue at the presence of H 2 S, and its fluorescence intensity ratio (I 460 nm /I 690 nm ) enhanced up by 540 folds. The TIBI -loaded test strips could successfully achieved the real-time and convenient monitoring of H 2 S produced from food spoilage . Besides, probe TIBI could be also applied for imaging H 2 S level in living cells and zebrafish.
研究领域:
生物化学
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分析化学
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食品安全
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细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2025
·
IF 3.3
Xu Zhenbo,Chen Rong,Wu Jie,Zhong Feifeng,Xue Haoyue,Xiang Zhufang,Yang He,Yuan Lei,Liu Junyan,Yu Junhong
Lacticaseibacillus paracasei is a typical food spoilage bacterium especially in beer. Due to the negative impact of L. paracasei on food preservation, a rapid detection method for L. paracasei based on polymerase spiral reaction (PSR) was established and its application was verified using different types of food samples in this study. Firstly, target digging was performed using bioinformatics techniques. A target gene pheS was selected and species-specific primers were designed. Secondly, the PSR assay was established with chromogenic reaction, which avoid false positive result due to open lid. The specificity of target and primers was tested, and the limit of detection (Kolodkin-Gal in Science 328:627–629, 2010) (5.2 × 10 4 fg/μL) was examined. Thirdly, the application of PSR assay in solid and liquid food samples were verified by artificial contamination model. The PSR assay was more efficiency in liquid food (LOD: 10 3 CFU/mL) than in solid food (LOD: 10 4 CFU/mL). Thus, L. paracasei screening in 3 types of beer (LOD: 10 3 CFU/mL) was further performed to verify to efficiency of PSR assay in screening of beer spoilage bacteria. In conclusion, pheS targeting PSR assay is useful as a rapid detection method for L. paracasei in solid and liquid food samples especially in beer.
研究领域:
微生物学
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食品安全
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
TheScientificWorldJOURNAL
·
2013
·
IF 1.73
Jun Tian,Xiaobin Zeng,Hong Zeng,Zhaozhong Feng,Xiangmin Miao,Xue Peng
The antifungal efficacy of nerol (NEL) has been proved against Aspergillus flavus by using in vitro and in vivo tests. The mycelial growth of A. flavus was completely inhibited at concentrations of 0.8 μL/mL and 0.1 μL/mL NEL in the air at contact and vapor conditions, respectively. The NEL also had an evident inhibitory effect on spore germination in A. flavus along with NEL concentration as well as time-dependent kinetic inhibition. The NEL presented noticeable inhibition on dry mycelium weight and synthesis of aflatoxin B1 (AFB1) by A. flavus, totally restraining AFB1 production at 0.6 μL/mL. In real food system, the efficacy of the NEL on resistance to decay development in cherry tomatoes was investigated in vivo by exposing inoculated and control fruit groups to NEL vapor at different concentration. NEL vapors at 0.1 μL/mL air concentration significantly reduced artificially contaminated A. flavus and a broad spectrum of fungal microbiota. Results obtained from presented study showed that the NEL had a great antifungal activity and could be considered as a benefit and safe tool to control food spoilage.
研究领域:
微生物学
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食品安全
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Jiaying Geng,Zhongxi Huang,Qian Shen,Chenfeng Xu,Long Zhang,Cuilan Wei,Chan Fu,Weizhen An,Changmin Yu
With the emergence of numerous food safety problems, rapid and accurate detection of histamine in food spoilage remains a challenge. To this end, we developed a simple design and easy synthesis of fluorescein-based probe FCHO to achieve specific and rapid (<1 s) quantitative detection of histamine through “imine formation” reaction. Significant enhanced fluorescence signal in response to histamine enabled our probe with high sensitivity as low as 51 nM. Utilizing the visualized fluorescence color changes of the probe as histamine increasing, we combined it with paper-based test chip to construct a color-resolved and highly selective recognition system. In addition, our proposed probe has been successfully used to visually imaging histamine changes in fish samples. Finally, for the first time, we have proved it possesses reliable ability to directly in situ imaging the distribution of histamine in whole spoiled fish. Thus, our strategy will provide great potential for monitoring food spoilage.
研究领域:
食品安全
·
分析化学
·
生物传感
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 7
·932
·
IF 5.561
Xujing Guo,Xirui Chen,Rui Chen,Yujie Tu,Tianying Lu,Yuqian Guo,Liang Guo,Yonghua Xiong,Xiaolin Huang,Ben Zhong Tang
Herein, we developed a paper-based smart sensing chip for the real-time, visual, and non-destructive monitoring of food freshness using a ratiometric aggregation-induced emission (AIE) luminogen (i.e., H+MQ, protonated 4-(triphenylamine)styryl)quinoxalin-2(1H)-one) as pH sensitive indicators. Upon exposure to amine vapors, the deprotonation of H+MQ occurs and triggers its color change from blue to yellow, with the fluorescence redshift from blue to amaranth. Consequently, we successfully achieved the sensitive detection of ammonia vapors by recording the bimodal color and fluorescence changes. Given the high sensitivity of H+MQ to ammonia vapor, a paper-based smart sensor chip was prepared by depositing H+MQ on the commercial qualitative filter paper through a physical deposition strategy. After being placed inside the sealed containers, the developed H+MQ-loaded paper chip was applied to the real-time monitoring of biogenic amine contents according to its color difference and ratio fluorescence change. The detection results were further compared with those obtained by the high-performance liquid chromatography method, which verified the feasibility of the designed paper chip for the food spoilage degree evaluation. Briefly, this work indicates that the designed H+MQ-loaded paper chip could be a promising approach for improving food freshness monitoring.Keywords:aggregation-induced emission;fluorescent sensor;paper chip;biogenic amines;food spoilage
研究领域:
分析化学
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食品安全
·
传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Tianran Lin,Yarong Wu,Zhihong Li,Zhiping Song,Liangqia Guo,Fengfu Fu
Colorimetric detection of biogenic amines, well-known indicators of food spoilage, plays an important role for monitoring of food safety. However, common colorimetric sensors for biogenic amines suffer from low color resolution or complicated design and intricate output for the end-users. Herein, we explored a simple but effective strategy for visual monitoring of biogenic amines with multiple color change based on hydrolysis-induced silver metallization reaction to tune the localized surface plasmon resonance (LSPR) adsorption of Au nanorods (NRs). The color change and blue shift of longitudinal LSPR peak of Au NRs were closely related to the concentration of biogenic amines. This strategy provided a simple, sensitive, robust, nondestructive, cost-effective, and user-friendly platform for in situ evaluating the freshness of foodstuffs.
研究领域:
食品安全
·
纳米技术
·
分析化学
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Peng Qin,Shun-Hui Liu,Chong-Yu Wang,Xin Kang,Fengdong Qu,Zhen-Hua Ge,Xuetao Gan
Bismuth sulfide (Bi2S3) has significant potential for gas sensing applications, but its performance is often limited by insufficient active surface sites and suboptimal response efficiency. This study aims to overcome these challenges by developing a halogen-doped Bi2S3 nanostructure sensor array for detecting key gases associated with food spoilage. Halogen doping (Cl, Br, and I) substantially enhances the electronic structure and surface activity of Bi2S3, improving both its selectivity and adsorption properties for target gases. Theoretical calculations reveal that halogen doping not only introduces primary adsorption sites on Bi2S3 nanomaterials but also facilitates electron transfer between gas molecules and the material. The sensor array exhibits excellent sensitivity and accuracy in detecting NH3, H2S, and CO gases, achieving an estimation error of only 0.8% for NH3 concentration when combined with machine learning algorithms. Furthermore, the array successfully distinguishes 18 different gas concentrations and classes, achieving a classification accuracy of 97.8%. The study also investigates the impact of humidity on sensor performance, revealing that Bi2S3–Cl nanorods maintain high NH3 adsorption properties under humid conditions. Practical application in beef freshness detection confirms the sensor array’s potential for real-world use, offering innovative solutions for food quality monitoring.
研究领域:
材料科学
·
食品科学
·
传感技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-8
·
IF 6.408
Wu Jinmei,Lu Qi,Fu Xiao,Xu Huajian,Wan Pingping,Fu Huimin,Ding Jiqiang,Zhang Jing,Mei Qingsong
A novel nanoassembly was constructed through encapsulating upconversion nanoparticles (UCNPs) into a metal–organic framework structure (ZIF-8), in which doxorubicin (DOX) was absorbed into pores of ZIF-8. The blue emission of UCNPs was quenched by DOX through the fluorescence resonance energy transfer (FRET) strategy. When the nanoprobe was exposed to food samples with different pH values, ZIF-8 collapsed to release DOX molecules, resulting in upconversion recovery. The porous structure of ZIF-8 provides abundant space for DOX absorption, which significantly improves the detection capacities and accuracy. It is shown that the probe has a good linear relationship when pH values vary from 2.5 to 7.4, and can distinguish pH variations as low as 0.5 in real samples. This strategy has been successfully used to determine food spoilage by determination of pH variations. Graphical abstract
研究领域:
分析化学
·
食品科学
·
纳米生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Siyao Luo,Chang-Ying Hu,Xiaowen Xu
Hydrogels are a promising option for detecting food spoilage in humid conditions, but current indicators are prone to mechanical flaws, posing a concern for packaging systems that require strong mechanical properties. Herein, a double network hydrogel was prepared by polymerizing methacrylamide in a chitosan system with aluminum chloride and glycerol. The resulting hydrogel demonstrated high stretchability (strain >1500 %), notch insensitivity, excellent fatigue resistance, and exceptional anti-freezing capabilities even at −21 °C. When incorporating bromothymol blue (BB) or methyl red (MR), or mixtures of these dyes into the hydrogels as indicators, they exhibited sensitive colorimetric responses to pH and NH 3 levels at different temperatures. Hydrogels immobilizing BB to MR ratios of 1:1 and 1:2 displayed clearer and more sensitive color responses when packed into chicken breast, with a sensitivity level of 1.5 ppm of total volatile basic nitrogen (TVB-N). This color response correlated positively with the accumulation of TVB-N on the packaging during storage at both 25 °C and 4 °C, providing sensitive indications of chicken breast deterioration. Overall, the developed hydrogels and indicators demonstrate enhanced performance characteristics, including excellent mechanical strength and highly NH 3 -sensitive color responses, making significant contributions to the food spoilage detection and intelligent packaging systems field.
研究领域:
材料科学
·
食品安全
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）