FOOD AND AGRICULTURAL IMMUNOLOGY
·
2014
·
IF 0.984
Juan Peng,Xu Meng,Xiaofang Deng,Jianping Zhu,Hua Kuang,Chuanlai Xu
Hen egg is one of the most frequent causes of food allergy in infants and adults. Ovalbumin (OVA) has been identified as a major egg allergen. In order to detect OVA in foods, a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAbs) was established. The 2 mAbs were selected out of 17 murine hybridomas secreting OVA-specific antibody. Using mAb17 as the capture antibody and mAb15 as the detection antibody, the detection limit of the ELISA method was 0.51 ng/mL, and the linear dynamic range was between 1.95 and 500 ng/mL. The recovery ranged from 85.6 to 115.2%, whereas the intra- and inter-assay coefficients of variation were less than 8.6 and 13.9%, respectively. Sample analysis verified that the produced anti-OVA mAb and the developed ELISA may provide a valuable tool for the sensitive determination of OVA in processed foods and for future studies on the mechanism of how OVA functions in anaphylaxis.
研究领域:
免疫学
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食品科学
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医学
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产品关联
DOI
高影响因子
Food & Function
·
2024
·
IF 5.1
Xing Zhang,xiao chen,Fan Yang,Huming Shao,Tianliang Bai,Xuanyi Meng,Yong Wu,Anshu Yang,Hongbing Chen,Xin Li
The exposure of food allergens elicits fast changes in the intestinal microenvironment, which guides the development of allergic reactions. Investigating the key information of these changes may help in better understanding food allergy. In this research, we explored the relationship between food allergy and extracellular adenosine triphosphate (ATP), a danger molecule that has been proven to regulate the onset of allergic asthma and dermatitis but has not been studied in food allergy, by building a unique animal model based on allergen-containing diet feeding. After 7-day consumption of an allergen-containing diet, the allergic mice showed severe enteritis with raised luminal ATP levels. The luminal dysregulated ATP deteriorated food-induced enteritis by boosting Th17 cell responses and promoting mucosal neutrophil accumulation. In vitro experiments indicated that the intervention of ATP promoted the differentiation of Th17 cells and the activation of neutrophils. In addition, the diet-induced allergy showed noticeable gut dysbiosis, characterized by decreased microbial diversity and increased diet-specific microbiota signatures. As the first, we show that the food-induced enteritis is associated with an elevated concentration of luminal ATP. The dysregulated extracellular ATP exacerbated the enteritis of mice to food challenge by manipulating intestinal Th17 cells and neutrophils.
研究领域:
免疫学
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医学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Cheng Li,Yu Lu,Zhijun Zhang,Linjuan Huang,Zhongfu Wang
The highly sulfated polysaccharide sea cucumber chondroitin sulfate (SCCS) can alleviate intestinal damage and display strong anti-food-allergic activity. The O -glycopattern levels in colonic mucin are closely related to the its protective effect on function of the intestinal barrier. However, the effect of the SCCS on colonic mucin O -glycan has not been investigated. In this study, ovalbumin (OVA)-sensitized allergic mice and SCCS treatment were used. Mouse colonic mucin O -glycome was released and analyzed through reductive β-elimination combined with PGC–LC–MS. A total of presumptive 20 neutral and 28 acidic O -glycan structures were identified, in which the core 2 type acidic O -glycan structure is predominant in Balb/c female mice. Treatment with OVA and SCCS did not change the numbers of colon mucin O -glycan type, but the expression level of total O -glycosylation was more abundant in the SCCS group mice than in the OVA group (1.8-fold), especially for acidic O -glycans (co-modified by fucose and sulfate groups). Furthermore, supplementation with SCCS reversed most of the O -glycan decreasing trend, which may be associated with a return to healthy levels of gut microbiota. In conclusion, our results demonstrate that SCCS could restore colonic mucin O -glycosylation levels and intestinal homeostasis and contribute to enhancing intestinal barrier function.
研究领域:
生物化学
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免疫学
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营养学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 16
·7375-7386
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IF 6.1
Cheng Li,Yang Tian,Jiahuan Pei,Yuyang Zhang,Daokuan Hao,Tianjiao Han,Xiaoqin Wang,Shuang Song,Linjuan Huang,Zhongfu Wang
Previous research studies have shown that sulfated polysaccharides can inhibit food allergy, but the detailed mechanism remains largely unknown. In this study, RBL-2H3 cells were used to compare the anti-allergic activities of four sulfated polysaccharides, and an ovalbumin (OVA)-sensitized allergic mouse experiment was used to explore their desensitization effect, with regard to the alteration in gut microbiota and immune cell differentiation. Compared with the shark, bovine and porcine chondroitin sulfate, sea cucumber chondroitin sulfate (SCCS) significantly inhibited the degranulation of RBL-2H3 cells. SCCS reduced allergic symptoms and protected the jejunum from injury in mice. Furthermore, SCCS increased the relative abundance of Lachnospiraceae NK4A136, decreased the relative proportion of Prevotellaceae NK3B31, and up-regulated the secretion of short chain fatty acids such as butyric acid in the feces, resulting in an increase in the mucin 2 (MUC2) secretion by goblet cells HT-29. Meanwhile, SCCS induced the differentiation of regulatory T cells in the mesenteric lymph nodes of mice. This study provides a deeper understanding of the functioning mechanism of SCCS in alleviating food allergy and may guide the development and production of anti-allergy active ingredients.
研究领域:
免疫学
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营养学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zhencong Yang,Xue Feng,Philippe Madjirebaye,Yuting Fan,Li Yan,Zhoujin Xu,Xuli Wu
Although sodium nitrite (SN) is broadly used as a food preservative, its immunomodulatory effects on food allergy pathogenesis remain poorly understood. This study examined how subchronic SN exposure influences dendritic cell (DC)-mediated sensitization and T-cell polarization in an Ovalbumin (OVA)-induced mouse food allergy (FA) model. Our findings reveal that SN exacerbated allergic responses manifested by increased clinical symptoms, reduced weight gain, and elevated serum histamine, MCP-1, and IgE levels compared to those of OVA-sensitization alone. Mechanistically, SN promoted Th2 polarization through T-bet/GATA3 imbalance, Treg suppression, and enhanced DC activation. In vitro studies have demonstrated augmented antigen uptake by SN, MHC II expression, and selectively activated MAPK (p-JNK/ERK1/2) and STAT6 pathways, amplifying Th2 cytokine production and IgE class switching. These findings indicate that SN might disrupt immune homeostasis through coordinated modulation of DC function, Th2 polarization, and regulatory mechanisms, which highlights the potential of SN to exacerbate FA via multiple immunomodulatory pathways.
研究领域:
免疫学
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过敏与免疫学
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分子免疫学
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营养免疫学
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黏膜免疫学
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信号转导
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食品毒理学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 24
·2200455
·
IF 6.575
Yuanjie Guo,Yu Ma,Liyuan Ma,Zixin Guo,Yingliang Xiao,Yunjun Liu,Jie Li,Shengnan Wang,Yixiang Liu
Scope This study assesses whether oleuropein prevents ovalbumin (OVA)-induced food allergy (FA) and investigates the underlying mechanisms. Methods and results A Balb/c FA mouse model is established and maintained for 7 weeks. The subjects are administered OVA by oral gavage to induce FA and supplemented with different oleuropein doses (1.00–20.00 mg kg −1 per day) to evaluate its preventative efficacy. The results indicate that oleuropein effectively alleviates OVA-induced allergy symptoms and promotes temperature elevation in sensitized mice. The secretion of serology-specific OVA-immunoglobulin (Ig)E, OVA-IgG, and histamine is inhibited in the sensitized mice. Oleuropein dramatically upregulates the expression of intestinal tight junction (TJ) proteins, regenerating gene (Reg) IIIγ, and interleukin (IL)-22, enhancing the physical and biochemical barrier function of the intestinal epithelium. Additionally, oleuropein improves the immune homeostasis of the intestinal epithelium by affecting the function of mucosal mast cells and regulatory T (Treg) cells. The disordered intestinal flora of the sensitized mice also improves after oleuropein administration. Conclusions These findings suggest that oleuropein prevents FA by enhancing intestinal epithelial barrier function and improving immune homeostasis and intestinal flora in sensitized mice. Therefore, diets rich in oleuropein should be recommended for people with FA.
研究领域:
免疫学
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胃肠病学
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营养学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2019
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IF 3.197
Jingyi Ma,Juan Zhang,Qiuhong Li,Zhaoling Shi,Huajie Wu,Huiqin Zhang,Liping Tang,Ru Yi,Hui Su,Xin Sun
Although the prevalence of food allergies is rising, the standard of care for these allergies is suboptimal. Probiotics have been reported to regulate the allergic immune response, but the underlying mechanisms are not fully understood. In this study, we aimed to evaluate the effect of a mixture of probiotics on food allergy in an ovalbumin (OVA)-induced murine model. Treatment with the probiotics attenuated OVA-induced allergic symptoms in mice. Flow cytometry analysis showed that oral administration of probiotics induced mucosal CD103 + dendritic cells (DCs), which promoted differentiation of regulatory T cells (Tregs). 16S rRNA analysis revealed that the probiotics modulated the composition of microbiota, especially by increasing the proportion of the Deferribacteres and Verrucomicrobia phyla and the Mucispirillum and Clostridium XlVa genera, which in turn regulated the immune system. Our findings provide molecular insight into the application of a mixture of probiotics to alleviate food allergies and even gut immune homeostasis.
研究领域:
免疫学
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微生物学
·
营养学
✓ 已验证
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Lu Zeng,Shanshan Song,Qiankun Zheng,Pengjie Luo,Xiaoling Wu,Hua Kuang
Shrimp tropomyosin is one of the most important causes of shellfish allergy. The objective of this study was to develop a highly sensitive and specific immunoassay for the detection of shrimp tropomyosin in food. Ten monoclonal antibodies against shrimp tropomyosin were obtained by fusion and cell screening. We found two optimum monoclonal antibodies (mAbs); mAb 2 was used as the capture antibody, and HRP-labelled mAb 3 was used as the detection antibody. Using this pair of mAbs, a sandwich enzyme-linked immunosorbent assay (ELISA) was developed with the limit of detection (LOD) of 0.45 ng/mL. Cross-reactivity with other food allergens using this method was found to be negligible. An immunochromatographic assay strip was also developed for the rapid detection of shrimp tropomyosin with the LOD of 0.5 ng/mL. The results demonstrated that our developmental methods represent a useful tool for the detection of shrimp tropomyosin in food.
研究领域:
免疫学
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食品安全
·
生物技术
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Huan Li,Jiesi Feng,Yanbo Wang,Guangming Liu,Xiaojing Chen,Linglin Fu
DNA-based detection methods are highly promising for risk assessment in the food sector, such as tracing the existence of food allergens. However, due to the complexity of food matrices, cumbersome protocols are often needed to isolate the DNA components, which hinder the achievement of rapid and on-site detection. Herein, an instant and multiple DNA extraction method was developed based on the poly(vinyl alcohol) microneedle (MN) patch. With simple press and peel-off operations within 1 min, samples suitable for DNA-based analysis such as polymerase chain reaction (PCR) could be collected. By further combining with the recombinase polymerase amplification assay, rapid screening of the allergenic risks in complex samples such as shrimp ball and cheesecake could be achieved within 30 min. The MN-based DNA extraction method not only was a potential alternative to the traditional DNA extraction method but provided a transformative approach in realizing rapid, on-site detection of foodborne hazards in collaborating with fast DNA-based assays.
研究领域:
食品科学
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生物技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ALLERGY
·
2021
·Vol. 77
·期 2
·483-498
·
IF 13.146
Renlan Wu,Xiefang Yuan,Xingjie Li,Ning Ma,Hongyu Jiang,Hongmei Tang,Guofeng Xu,Zhigang Liu,Zongde Zhang
Background Alteration of commensal microbiota is highly correlated with the prevalence of allergic reactions to food in the gastrointestinal tract. The mechanisms by which microbiota modulate food allergen sensitization in the mucosal site are not fully understood. Methods We generate DCs specific knockout of retinoic acid receptor α (Rara) gene mice (DC KO Rara) to evaluate food sensitization. The bile acid-activated retinoic acid response was evaluated by flow cytometry, real-time RT-PCR and Illumina transcriptome sequencing. The global effect of Abx treatment on BA profiles in the mucosal lymph tissue mLN in mice was examined by UPLC-MS analysis. Results In this study, we demonstrate that depletion of commensal gut bacteria leads to enhanced retinoic acid (RA) signaling in mucosal dendritic cells (DCs). RA signaling in DCs is required for the production of food allergen-specific IgE and IgG1. Antibiotics induced an enlarged bile acid (BA) pool, and dysregulated BA profiles contributed to enhanced RA signaling in mucosal DCs. BA-activated RA signaling promoted DC upregulation of interferon I signature, RA signature, OX40L, and PDL2, which may lead to T helper 2 differentiation of CD4+ T cells. BA-activated RA signaling involved the farnesoid X receptor and RA receptor α (RARa) interaction. Depletion of bile acid reduces food allergen specific IgE and IgG1 levels in mice. Conclusion Our research unveils a mechanism of food sensitization modulated by BA-RA signaling in DCs, which suggests a potential new approach for the intervention of food allergic reactions.
研究领域:
免疫学
·
微生物学
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过敏
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分子生物学
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DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 7
·3934-3941
·
IF 3.241
Ziye Zhang,Hang Xiao,Xiaofeng Zhang,Peng Zhou
Shrimp tropomyosin (TM) is a glycoprotein allergen with high lysine content, however, there is little data on how deglycosylation and glycation modify the allergenicity of TM. In this work, the effects of deglycosylation (enzymatic and chemical) and glycation of TM by glucose on in vivo allergenicity and mast cell function were investigated using BALB/c mouse and RBL-2H3 mast cells. Compared with TM, after deglycosylation, the deglycosylated TMs (DGTMs) exacerbated the in vivo allergenicity and mast cell allergy response. As for the TM glycated by glucose (TM-G), glycation led to weaker allergy responses on mouse and mast cells, which might be due to glucose destroying the epitopes with glycation sites. Moreover, the glucose residues on TM-G functioned as both a resistance to prevent the accessibility of TM-G to the IgE on mast cell and masking epitopes. Therefore, deglycosylation of TM was not suitable for desensitizing TM-induced food allergy, while glycation of TM might be applied to desensitize TM allergenicity, which could provide new insight into food allergy desensitization for larger cohorts of shrimp-allergic patients.
研究领域:
免疫学
·
食品科学
·
过敏研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chenglin Zhang,Ya Wang,Qian Zhang,Baolian Liu,Xiupei Yang
The accurate determination of β-Lactoglobulin (β-LG), a major dairy allergen, is essential for susceptible individuals' health. Here, a multimodal β-LG sensor was developed by utilizing the aptamer (Apt) to regulate the metal-organic framework UiO-66-TCPP’ functions. The prepared UiO-66-TCPP had both fluorescence and peroxidase activity. After adsorbing β-LG Apt, the catalytic activity of UiO-66-TCPP was enhanced through increased substrate affinity and electron transfer, while the fluorescence of 6-carboxyfluorescein-labeled Apt was quenched via multiple interactions with the material, and the fluorescence of UiO-66-TCPP itself remained unchanged. After binding to β-LG, Apt desorbed from UiO-66-TCPP surface, thereby restoring Apt fluorescence and lowering UiO-66-TCPP’ catalytic activity. Thus, a colorimetric/ratiometric fluorescence platform for β-LG sensing in dairy products was constructed with low limits of detection (16.19, 47.69 ng/mL). On-site visual detection was further achieved by RGB analysis on a smartphone. This study proposes novel approaches to creating multi-modal platforms for accurate food allergen determination.
研究领域:
食品安全
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分析化学
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生物传感器
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纳米材料
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过敏原检测
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）