MOLECULES
·
2019
·Vol. 24
·期 8
·1593
·
IF 3.06
Xin-Jun Yu,Chang-Yi Huang,Hong Chen,Dong-Sheng Wang,Jing-Liang Chen,Hui-Juan Li,Xiao-Yan Liu,Zhao Wang,Jie Sun,Zhi-Peng Wang
The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protistAurantiochytriumsp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109Aurantiochytriumsp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the twoAurantiochytriumsp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginousAurantiochytriumsp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.Keywords:Aurantiochytriumsp., carbohydrate;DHA;feed;FT-IR;high-throughput
研究领域:
生物技术
·
海洋生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Zhiming Xiao,Ruiguo Wang,Decheng Suo,Shi Wang,Xiaomin Li,Shujun Dong,Tong Li,Xiaoou Su
Increasing concerns over bisphenol A (BPA) as an endocrine disrupting chemical (EDC) and its adverse effects on both humans and animals have led to the substitution by structural analogs, such as bisphenol F (BPF), in many application areas. Information regarding to the carry-over of this emerging chemical in farm animals is essential for legislation and risk assessment purposes. In this study, a large-scale number of animal experiments were designed to investigate the transfer of BPF from feed to eggs. One control and three experimental groups of laying hens (72 hens per group) were fed with basal diets and BPF-contaminated feed at concentration levels of 0.1, 0.5 and 2.5 mg kg −1 , respectively, for two weeks. The hens were then fed with BPF-free diets for a further four weeks. Eggs were collected daily, and separated into egg yolk and white for BPF analysis. The effects of different levels of BPF exposure on laying performance followed a non-monotonic dose-response curve, since low level BPF (0.1 mg kg −1 ) exposure did increase the laying rate, mean egg weight and daily feed intake, while high level BPF (2.5 mg kg −1 ) exposure showed a decreasing trend. BPF residues were detected in both egg yolks and whole eggs after two days of administration, and plateau phase was achieved within 9–18 days. There are clear linear dose-response relationships between the plateau BPF concentrations in feed and eggs. The residue of BPF was found mainly in egg yolks with conjugated form and depleted slowly (still detected 21 days after feeding the BPF-free diet of the high level group). Mean carry-over rate of 0.59% BPF from feed to eggs was obtained. Compared with the carry-over rates of PCBs and dioxins, BPF showed a relatively minor trend of bioaccumulation in eggs. To the best of our knowledge, this is the first report on the deposition, depletion, and bioaccumulation study of bisphenols in farm animals. The quantity of data can therefore be helpful in the frame of risk assessment, especially for a comprehensive estimation of consumer exposure to the residues of bisphenols.
研究领域:
动物科学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Zhenjiang Yang,Suiliang Huang,Wenwen Kong,Liang Chen,Priakhina Ekaterina,Zobia Khatoon,Muhammad Nabil Ashraf,Waseem Akram
The number of undesirable environmental impacts of fish feed has been reported widely. Although repeated fish feed exposures are more prospective to occur in water, previous studies were mostly conducted as a single exposure of fish feed. In order to fill these gaps, a 40 days incubator experiment was conducted to explore the effects of fish feed addition scenarios during the lag phase with prometryn on both Microcystis aeruginosa growth and concentrations of nutrients. The maximum algae densities in groups of single exposure were 6.0–26.2% and 8.8–74.4% higher than those in groups of double and triple exposures, respectively ( P < 0.05). At the beginning of the experiment, concentrations of nutrients in groups with different feed exposure scenarios were significantly different. The pattern of nutrient limitation showed a transformation from phosphorus limitation to nitrogen limitation generally. Furthermore, the average inhibition rates of algae by prometryn in the case of a single fish feed exposure were 4.6–9.4% lower than those under double exposures, and 22.0–26.8% lower than those under triple exposures ( P < 0.05). In addition, algae growth rates have been developed as a function of concentrations of consumed nutrients ( R 2 = 0.410–0.932). Based on the above results, we concluded that in terms of limiting algae growth multiple low-dosage additions of fish feed were considered as a better addition pattern. By optimizing feed addition scenarios, there is considerable potential to increase the environmental sustainability of aquaculture.
研究领域:
环境科学
·
水生生物学
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Hong Liao,Wenjun Ying,Zhina Lian,Yong Xu,Junhua Zhang
Acid salts have been shown to catalyze xylan hydrolysis selectively and efficiently for xylooligosaccharides (XOS) production while using acid salts that are non-toxic and available as feed additives can avoid separation from resulting XOS-rich hydrolysates. There is no report on XOS production with sodium bisulfate (NaHSO 4 ) hydrolysis, of significance is that NaHSO 4 as feed additive does not need to be separated. In this work, NaHSO 4 hydrolysis was firstly employed to produce XOS from poplar. XOS yield of 42.7% was reached under optimal conditions of 0.04 mol/L NaHSO 4 , 170 °C and 60 min. After hydrogen peroxide/acetic acid and sodium hydroxide treatments of NaHSO 4 -pretreated poplar, high yields of glucose (92.0%) and xylose (91.3%) were obtained at a low cellulase dose of 5 FPU/g dry mass. NaHSO 4 hydrolysis was a novel strategy to prepare XOS efficiently with simple operation steps, and XOS-rich hydrolysates could be potentially used as feed additives without NaHSO 4 separation.
研究领域:
生物化学
·
可再生资源与环境工程
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2012
·Vol. 5
·期 1
·59-63
·
IF 1.547
Suo Decheng,Li Lan,Zhang Su,Su Xiaoou
A simple, highly sensitive ion chromatographic method for the simultaneous determination of betaine, choline and carnitine in feed premixes is described. Premixes were extracted via ultrasonication in water for 20 min and extracts were analyzed by ion chromatography using an aqueous 3.0 mmol L−1 methane sulfonic acid solution containing 15% (v/v) acetonitrile as the eluent and an IonPac SCS1 column. The composition of the mobile phase was optimized for the efficient separation of the three main analytes from each other as well as from common inorganic cations and trimethylamine, which is an ingredient for the synthesis of the analytes. The recoveries of choline, carnitine and betaine spikes added to premixes were all greater than 90% with replicate relative standard deviations of less than 10%. The limits of detection of this method for these analytes in premixes were calculated to be 10, 6 and 20 mg kg−1, respectively.
研究领域:
分析化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 17
·3578-3586
·
IF 1.915
Haijing Du,Yanxin Chu,Hong Yang,Kang Zhao,Jianguo Li,Pei She,Xiang Zhang,Anping Deng
Brombuterol, a new β-adrenergic agonist which can enhance the lean meat-to-fat ratio, is forbidden as an additive in animal feeds for livestock production due to its adverse effects on consumers. In this study, a highly sensitive and specific competitive enzyme-linked immunosorbent assay (ELISA) for the detection of brombuterol in tissue and feed samples was developed. The immunogen and coating antigen were prepared by directly linking brombuterol to carrier proteins using the diazobenzidine method. The antisera against brombuterol were obtained from immunized rabbits. The IC50 and limit of detection (LOD) values of the superior ELISA were 0.165 ng mL−1 and 0.007 ng mL−1, respectively. The cross-reactivity (CR) values of ELISA with clenbuterol, terbutaline and cimbuterol were less than 6.2%; there was no CR of ELISA with nine other compounds. To investigate the accuracy and precision of the assay, liver, meat and feed samples were spiked with brombuterol at different concentrations and analyzed by ELISA. Acceptable recovery rates of 91.9–115.4% and intra-assay coefficients of variation of 1.5–9.5% (n = 3) were achieved. The ELISA was also validated by HPLC. As revealed, both methods were highly correlated (R2 = 0.9927–0.9994, n = 6). The proposed ELISA was proven to be a feasible method for quantitative analysis of brombuterol in tissue and feed samples.
研究领域:
食品安全
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Juan-ying Li,Yuxiao Tong,Qi Gong,Yaxiong Tian,Yanhao Yang,Luting Wen,Hongjie Chen,Qian Wang
Eastern China is an important area for aquaculture, but the aquaculture environment, as well as the aquatic product quality, are threatened by environmental pollutants such as polycyclic aromatic hydrocarbon (PAHs) and pyrethroid pesticides (PYRs). A three-month monitoring program for PAHs and PYRs throughout the whole culture processes of the shrimp Penaeus vanmamei was carried out. Specifically, concentrations of PAHs and PYRs ranged from 339 ± 19.5–631 ± 75.6 ng·L −1 and 65.3 ± 6.74–93.5 ± 10.8 ng·L −1 in water, 183 ± 33.8–369 ± 25.1 ng·g −1 dw and 441 ± 132–521 ± 62.8 ng·g −1 dw in sediments, while 416 ± 84.3–646 ± 19.7 and 483 ± 23.3–513 ± 22.1 ng·g −1 dw in suspended solids. Mean concentrations of PAHs and PYRs in the adult shrimp were 634 ± 82.3 ng·g −1 ww and 665 ± 58.1 ng·g −1 ww, respectively. A health threat would arise if men consume more than 28.8 g and women consume more than 24.4 g each day. PAHs in the aquaculture environment were generally from the influent water and rainfall, while PYRs were mainly introduced by feed. After entering the culture environment, PAHs primarily increased the water load, leading to more significant bioaccumulation of the shrimp from the water phase (72–95 %). For PYRs, the main sources were feed (44.4 %) and influent water (20.6 %), and they were accumulated in shrimp through feed assimilation directly (15.0 %–32.0 %). Additionally, feed waste containing PYRs increased the contamination burden of sediments and suspended solids, leading to bioaccumulation through assimilation (4.00 %–15.0 %) as well. Although the contribution of influent water to PYRs in the culture environment was lower than that of feed, the contribution of aqueous exposure to the enrichment of PYRs in the shrimp was higher (53.0 %–81.0 %) than that of food (feed, sediments, and suspended solid) intake (19.0 %–47.0 %). The hydrophobicity of PYRs led to an easier bioaccumulation from freely dissolved phase to biota. Therefore, it is important to pay attention to the water quality during the whole culture period, as well as feed quality, to ultimately improve the quality of aquaculture products. In summary, different pollutants in the aquaculture environment and products were from different sources and exposure pathways. Water quality monitoring and improvement are more efficient in decreasing PAHs in the shrimp, while PYRs reduction should be assisted with feed quality control.
研究领域:
环境科学
·
水产养殖学
·
生态毒理学
✓ 已验证
产品关联
DOI
高影响因子
Insects
·
2025
·Vol. 16
·期 4
·380
·
IF 2.9
Tingting Zhang,Yinchen Yu,Jianyu Li,Li Zheng,Shiwei Chen,Jianjun Mao
Simple SummaryTheHarmonia axyridisis an important natural enemy insect that has received widespread attention in research and the application of biological pest control. Currently, suitable artificial feed for the mass-rearing of theH. axyridishas not been found, which affects the application of this natural enemy in biological control. This study found that the egg production of artificial feed without added egg yolk is similar to that of artificial feed without added egg yolk supplemented with juvenile hormone III. Compared to the control artificial feed, the egg production significantly increased, which is of great significance for further exploring the effects of non-insect source artificial feed on the growth and development of theH. axyridis.Artificial diets applied in the mass-rearing propagation ofH. axyridiscan improve reproductive ability by optimizing the feeding formula. This study used transcriptome data to investigate the effects of various artificial diets on the growth and development ofH. axyridis. Results indicate that spawning increased with the low-fat and JH III-supplemented artificial diet (Diet 3). Furthermore, the highest glycogen content found in Diet 3 was significantly different from the other two groups. Triglyceride content decreased as adult feeding time increased in the three artificial diet groups, with the fastest decrease observed in the low-fat diet (Diet 2). Protein content increased gradually in the high-fat diet (Diet 1) group compared to the other treatment groups. The adults reared on low-fat artificial diets, when compared to those on artificial diets supplemented with juvenile hormones at the transcriptome level, were found to have upregulated genes enriched in ubiquitin-mediated proteolysis, ribosome biogenesis, and the hedgehog signaling pathway. In contrast, the genes upregulated in the latter group were enriched in oxidative phosphorylation, amino acid biosynthesis, and the metabolism of other amino acids. The results suggest that nutritional status significantly affects the growth and development ofH. axyridisand has practical implications for the artificial feeding of natural pest enemies.
研究领域:
昆虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2012
·Vol. 34
·期 2
·277-283
·
IF 3.303
Libo Li,Hongwei Du,Huan Yu,Lei Xu,Tianyan You
CE coupled with amperometric detection method was developed using ionic liquid 1-ethyl-3-methylimidazolium tetrafluoroborate (EMImBF 4 ) as additive for the simultaneous detection of clenbuterol (CLB), terbutaline (TER), and ractopamine (RAC) in feed. The effects of detection potential, concentration of EMImBF 4 , pH, and concentration of the running buffer, separation voltage as well as injection time on the separation and detection of these three β -agonists were investigated in detail. Under the optimum conditions: the detection potential at 1.05 V, 50 mmol/L Tris-HAc at pH 8.0 with 0.6% (v/v) EMImBF 4 , electrokinetic injection 6 s at 16 kV and separation voltage at 16 kV, a baseline separation for these three analytes could be achieved within 11 min. Introduction of EMImBF 4 into the running buffer resulted in significant improvement in separation selectivity and enhancement in peak currents for those β -agonists, especially for TER and RAC, which could not be separated in the running buffer without additive. The method exhibited wide linear range with LOD (S/N = 3) of 2, 1, and 2 nmol/L for CLB, TER, and RAC, respectively. The precision was determined in both intraday ( n = 5) and interday ( n = 3) assays, and the RSDs for both migration time and peak current were less than 6%. The proposed method was also applied to analyze β -agonists in feed sample.
研究领域:
分析化学
·
兽医药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Fermentation-Basel
·
2022
·Vol. 8
·期 1
·25
·
IF 5.123
Wen-Tao Sun,Yuan Huang,Chang-Rong Wu,Chao Peng,Yu-Long Zheng,Chao Chen,Jun Hao
In this study, the influence of two lactic acid bacteria (LAB) strains [Lactobacillus rhamnosusBDy (LR-BDy) andLactobacillus buchneriTSy (LB-TSy)] selected from Southwest China on the fermentation characteristics and in vitro gas production ofBroussonetia papyrifera(paper mulberry) silage were experimentally explored. The experimental groups were a control group (C), an LB-TSy treatment (LB), an LR-BDy treatment (LR), and an LR-BDy + LB-TSy hybrid group (LR × LB). After the LAB were added, the pH value of paper mulberry silage significantly declined (p< 0.05), and the crude protein content was effectively preserved (p< 0.05). However, no significant changes were found in the levels of neutral detergent fiber, acid detergent fiber, and crude ash (p> 0.05). The lactic acid content in paper mulberry silage was evidently increased (p< 0.05). The in vitro gas production in the LR at 36, 48, and 72 h were markedly higher than that in the other treatments (p< 0.05). Owing to the addition of LAB, the microbial diversity in paper mulberry silage was reduced, while the relative bacterial abundance ofLactobacilluswas enhanced. Hence, the addition of LAB selected from the warm and humid region in Southwest China can improve the quality of paper mulberry silage and elevate its feeding value in this region.Keywords:paper mulberry;silage;in vitro gas production;microbial community;lactic acid bacteria
研究领域:
畜牧学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Li Xiaomei,Chen Fei,Xiong Yi,Guo Linna,Xu Jingjing,Lin Yanli,Ni Kuikui,Yang Fuyu
The aim of this study was to investigate the influence of Perilla frutescens, alone or in combination with Lactobacillus plantarum a214 or citric acid, on forage oat silage quality, bacterial and fungal microbiological profile during ensiling and aerobic exposure. With the exception of Perilla frutescens, all additives could improve silage quality of forage oat based on lower ammonia-nitrogen content and higher residual of water soluble carbohydrates during anaerobic fermentation compared to control silage, especially in Perilla frutescens combined with citric acid (CAPF). Lactobacillus was the dominant bacteria in all silages, while CAPF group increased the relative abundance of Lactobacillus lindneri and Lactobacillus brevis compared to control silage. The application of Perilla frutescens suppressed the relative abundance of yeasts such as Pichia fermentans and Wickerhamomyces anomalus in response to aerobic exposure, especially in CAPF treatment, leading to high acetic acids and lower dry matter loss, as well as good aerobic stability. Therefore, Perilla frutescens, alone or in combination with citric acid, has potential to improve aerobic stability of forage oat silage by shifting bacterial and fungal community composition, and can be used as new additive to prepare high-quality silage for animal production.
研究领域:
微生物学
·
动物营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbial Biotechnology
·
2020
·Vol. 13
·期 4
·1118-1131
·
IF 5.328
Tao Ma,W. Wu,Y. Tu,N. Zhang,Q. Diao
Summary This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high-concentrate (HC) and high-forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets ( P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter ( P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet ( P = 0.045). The relative abundance of 10 bacterial genera was affected by the three-way interaction of treatment, diet and time ( P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales . Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition.
研究领域:
动物科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）