JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Haichun Zeng,Jiatong Zeng,Beilin Meng,Jianmei Peng,Li Rao
Geraniol, an acyclic monoterpenoid of substantial value extracted from the essential oils of various aromatic plants, holds significant commercial and industrial importance in the realms of food, cosmetics, medicine, and bioenergy. Geraniol synthase, which is responsible for geraniol production, has been identified in only several plant species to date. Here, we present the first cloning and characterization of a geraniol synthase (PgfTPS) from Penicillium griseofulvum. This enzyme demonstrates pronounced specificity in catalyzing the conversion of geranyl diphosphate into geraniol. Moreover, through protein modeling and site-directed mutagenesis, we have identified key active-site residues crucial for the catalytic function of PgfTPS. Finally, we utilized engineered Saccharomyces cerevisiae as a host for PgfTPS expression to facilitate geraniol production. Our findings not only advance the development of efficient biocatalysts for geraniol generation but also establish a fundamental basis for further exploration into fungal monoterpene biosynthesis.
研究领域:
生物化学
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微生物学
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植物代谢工程
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DOI
PubMed
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INDUSTRIAL CROPS AND PRODUCTS
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2025
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IF 6.2
Chengshun Liu,Jianlong Li,Miao Wang,Guotai Jian,Chen Zhu,Hanxiang Li,Yongxia Jia,Jinchi Tang,Lanting Zeng
Camellia sinensis var. assamica is a major variety used to produce black tea. However, the core compounds influencing its aroma quality are unknown. In this study, Yinghong No. 9 black tea (YJBT), a distinctive black tea in South China, was analyzed. We detected 44 aroma compounds and identified seven key contributors, namely linalool, geraniol, trans -linalool oxide (furanoid), hexanal, methyl jasmonate, phenylacetaldehyde, and β-ionone. Linalool, which was generally more abundant in spring tea than in summer tea, contributed significantly to sweet and floral aromas. ( R )-Linalool was the primary linalool form in Yinghong No. 9, wherein it was highly abundant in high-quality tea leaves. The substantial expression of a linalool synthase gene and the relatively high catalytic efficiency of the encoded enzyme likely contributed to the accumulation of ( R )-linalool. This study identified the primary aroma compounds in YJBT, providing a foundation for advancing our knowledge of the aromatic quality of black tea.
研究领域:
植物学
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食品科学
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分子生物学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Rumeng Li,Bo Yao,Haichun Zeng
Nerol, a linear monoterpenoid, is naturally found in essential oils of various plants and is widely used in the fragrance, food, and cosmetic industries. Nerol synthase, essential for nerol biosynthesis, has previously been identified only in plants that use NPP as the precursor. In this study, a novel fungal nerol synthase, named PgfB, was cloned and characterized from Penicillium griseofulvum. In vitro enzymatic assays showed that PgfB could directly convert the substrate GPP into nerol. Furthermore, the successful expression of PgfB and its homologous protein in Saccharomyces cerevisiae resulted in the heterologous production of nerol. Finally, crucial amino acid residues for PgfB’s catalytic activity were identified through site-directed mutagenesis. This research broadens our understanding of fungal monoterpene synthases and presents precious gene resources for the industrial production of nerol.
研究领域:
真菌学
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生物化学
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合成生物学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
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IF 4.192
Ying Zhou,Rufang Deng,Xinlan Xu,Ziyin Yang
Linalool is abundant in tea leaves and contributes greatly to tea aroma. The two isomers of linalool, (R)-linalool and (S)-linalool, exist in tea leaves. Our study found that (R)-linalool was the minor isomer in nine of Camellia sinensis var. sinensis cultivars. The (R)-linalool synthase of tea plant CsRLIS was identified subsequently. It is a chloroplast-located protein and specifically catalyzes the formation of (R)-linalool in vitro and in vivo. CsRLIS was observed to be a stress-responsive gene and caused the accumulation of internal (R)-linalool during oolong tea manufacture, mechanical wounding, and insect attack. Further study demonstrated that the catalytic efficiency of CsRLIS was much lower than that of (S)-linalool synthase CsSLIS, which might explain the lower (R)-linalool proportion in C. sinensis var. sinensis cultivars. The relative expression levels of CsRLIS and CsSLIS may also affect the (R)-linalool proportions among C. sinensis var. sinensis cultivars. This information will help us understand differential distributions of chiral aroma compounds in tea.
研究领域:
植物学
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分子生物学
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芳香化合物代谢
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Lin Yue,Jingru Li,Wanwen Chen,Xiaoli Liu,Qixing Jiang,Wenshui Xia
The novel derivatives of geraniol grafted chitosan oligosaccharide were synthesized via substitution and deprotection reaction, respectively. The products were identified by Fourier transform infrared (FT-IR), 1 H nuclear magnetic resonance (NMR), X-ray diffraction analysis (XRD), Thermogravimetric analysis (TGA) and UV–vis absorption spectroscopy. It is revealed that the derivatives exhibited a good solubility, thermal stability and antibacterial properties.
研究领域:
生物材料学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2011
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IF 2.089
Hengde Li,Tianfei Zhang,Weiqin Fu,Kazuhiro Tamura
Liquid–liquid equilibrium (LLE) data of the binodal curves and tie-line compositions were examined for ternary mixtures of (water + methanol + geraniol) and (water + ethanol + geraniol) at T = (283.15, 298.15, and 313.15) K. The immiscible area of the (water + methanol + geraniol) system is wider than that for the ethanol system at the same temperature. The experimental liquid–liquid equilibrium data have been represented by using the original UNIQUAC, extended UNIQUAC, and modified UNIQUAC models.
研究领域:
化学工程
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物理化学
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DOI
高影响因子
NEW PHYTOLOGIST
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2019
·Vol. 226
·期 2
·362-372
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IF 7.299
Mingyue Zhao,Na Zhang,Ting Gao,Jieyang Jin,Tingting Jing,Jingming Wang,Yi Wu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
Summary Plants produce and emit terpenes, including sesquiterpenes, during growth and development, which serve different functions in plants. The sesquiterpene nerolidol has health-promoting properties and adds a floral scent to plants. However, the glycosylation mechanism of nerolidol and its biological roles in plants remained unknown. Sesquiterpene UDP-glucosyltransferases were selected by using metabolites-genes correlation analysis, and its roles in response to cold stress were studied. We discovered the first plant UGT (UGT91Q2) in tea plant, whose expression is strongly induced by cold stress and which specifically catalyzes the glucosylation of nerolidol. The accumulation of nerolidol glucoside was consistent with the expression level of UGT91Q2 in response to cold stress, as well as in different tea cultivars. The reactive oxygen species (ROS) scavenging capacity of nerolidol glucoside was significantly higher than that of free nerolidol. Down-regulation of UGT91Q2 resulted in reduced accumulation of nerolidol glucoside, ROS scavenging capacity and tea plant cold tolerance. Tea plants absorbed airborne nerolidol and converted it to its glucoside, subsequently enhancing tea plant cold stress tolerance. Nerolidol plays a role in response to cold stress as well as in triggering plant–plant communication in response to cold stress. Our findings reveal previously unidentified roles of volatiles in response to abiotic stress in plants.
研究领域:
植物学
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分子生物学
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环境科学
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DOI
PubMed
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BIOTECHNOLOGY AND BIOENGINEERING
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2025
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IF 3.6
Liang Li,Hui Jiang,Tingting Xu,Xi Wang
Citronellal and citronellol are monoterpenoids that serve as key ingredients in the flavor and fragrance industry and hold significant potential for pharmaceutical applications. Currently, these compounds are primarily obtained through chemical synthesis or extraction from plant essential oils. However, these methods raise concerns regarding environmental sustainability, carbon emissions, and the growing attentions for natural production. A promising alternative is microbial biosynthesis, which enables the natural and carbon-neutral production of these compounds in an environmentally friendly manner. In this study, we developed bienzymatic cascades for the efficient production of citronellal and citronellol from geraniol, utilizing E. coli 's endogenous glycerol dehydrogenase (GLDA), NADPH-dependent aldehyde reductase (AHR), and Catharanthus roseus iridoid synthase (CrIS). The highest in vitro production reached 92 mg/L of citronellal and 170 mg/L of citronellol in a 5-h enzymatic reaction. We also engineered these enzymatic cascades into E. coli strains for in vivo production, achieving up to 714 mg/L of citronellol. This study establishes new bienzymatic cascades for the efficient biosynthesis of citronellal and citronellol, demonstrating a possible bio-based production for natural flavor and fragrance compounds.
研究领域:
合成生物学
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生物催化
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代谢工程
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工业微生物学
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酶学
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PubMed
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
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IF 4.389
Jing Hu,Shanshan Liu,Weijun Deng
Linalool is a main component in different naturally derived essential oils, and widely used in household, personal care, food and therapeutic formulations. However, the application is limited due to its high volatility and low stability. In this study, an effective encapsulation with high loading ratio was built up together with thermal-redox dual responsiveness and controlled release properties. The emulsified linalool droplets were modified with carbon-carbon double bonds, followed by the precipitation polymerization with thermal sensitive monomer, N-vinyl caprolactam. The average size and the loading ratio of the prepared linalool capsules were 1.4 μm and 50.41 wt%. The linalool capsules exhibited thermal-redox dual responsive properties and the antioxidant-antibacterial performance. Especially, responding to the stimuli mimicking practical circumstance, the synthesized capsules presented excellent bacteria inhibiting effect. This work may open a new path for fragrance and essential oil encapsulation, enlarging them as the green biological antibacterial agents in different applications.
研究领域:
材料科学
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生物学
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DOI
PubMed
高影响因子
Results in Chemistry
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2025
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IF 4.2
Chengjia Bao,Weijian Qi,Zhuanjun Zhu,Ting Xu,Long Zhao,Yanhong Ma,Yang Liu,Bowen Yang,Xueru Luo,Hui Yun,Xiaojuan Li
Chitosan (Chi), curcumin (Cur) and geraniol (Ger) are natural compounds, which can potentially serve as green corrosion inhibitors. Herein, the corrosion inhibition efficiency (IE) of them was compared. The corrosion rate of 1.5 mM Chi on the 1 st d was 0.78 ± 0.41 g/ (m 2 h), significantly lower than that of the control group (CK) (2.59 ± 0.32 g/ (m 2 h)), while the corrosion rate of 2 mM Cur decreased by 8.48–18.71 times. The corrosion IE of 1.5 mM Ger remained above 60 % for over 15 days. To improve corrosion IE on Q235 steel in acidic solution, two-component and three-component compounds of them at their optimal IE concentrations were also tested and evaluated by weight loss and electrochemical analysis. Chi+Cur + Ger exhibited the minimum growth rate of corrosion rate (0.19–15.36 g/ (m 2 h)) and the maximum corrosion IE (81.87–96.41 %) during 20 days. The weight loss was 20.65 % of the control on the 20th day. The Tafel results indicated Chi+Cur + Ger functioned as a mixed-type corrosion inhibitor, inhibiting the cathodic reaction more significantly. The surface observation and X-ray photoelectron spectroscopy (XPS) proved the formation of an adsorption layer on the steel surface. Chi and Cur could adsorb on the steel surface through chemical interaction like by amine and/or hydroxyl groups, while Ger through physical adsorption. The synergistic anticorrosion layer not only prevented steel surface from corrosive solution attack, but also increased the resistance to retard the charge transfer in the solution. The results might shed new insight in the application of green complex for steel corrosion inhibition.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
Frontiers in Microbiology
·
2023
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IF 5.2
Li Wen-Ru,Zeng Tao-Hua,Zhang Zhi-Qing,Shi Qing-Shan,Xie Xiao-Bao
Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that can cause severe respiratory tract infections. Geraniol, a chemical component of essential oils, has antimicrobial and anti-inflammatory activities, along with low toxicity. However, the effect and mechanism of geraniol against P. aeruginosa virulence factors are rarely studied. In this study, we investigated the quorum sensing (QS) inhibitory effects and mechanisms of geraniol against P. aeruginosa PAO1, using physiological and biochemical techniques, quantitative reverse transcription polymerase chain reaction, and transcriptomics. Geraniol slightly affected P. aeruginosa PAO1 growth, prolonged the lag phase, and delayed growth periods in a concentration-dependent manner. Geraniol inhibited three QS systems of P. aeruginosa, las, rhl, and pqs by suppressing the expression level of their key genes, including the three signal synthetase encoding genes of lasI, rhlI, and pqsABCDEH, and the corresponding signal receptor encoding genes of lasR, rhlR, and pqsR. Geraniol also suppressed certain virulence genes regulated by these three QS systems, including rhlABC, lasAB, lecAB, phzABMS, and pelABG, resulting in the attenuation of the related virulence factors, rhamnolipids, exoprotease LasA, elastase, lectin, pyocyanin, and biofilm. In conclusion, geraniol can suppress the virulence factors of P. aeruginosa PAO1 by inhibiting the three QS systems of las, rhl, and pqs. This study is significant for improving the treatment of bacterial infections caused by P. aeruginosa.
研究领域:
微生物学
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药理学
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分子生物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2024
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IF 5
Xueying Song,Jiamu Kang,Xingyan Wei,Liu Liu,Yongfeng Liu,Feng Wang
Shigella flexneri has the ability to contaminate pork and cause foodborne diseases. This study aimed to examine the effectiveness of linalool (a natural preservative) against S. flexneri and explore its potential application in contaminated pork. The results showed that linalool was capable of damaging the cell membrane and binding to the DNA of S. flexneri , and inhibiting biofilm formation and disrupting mature biofilms. The antibacterial effectiveness of linalool on the surface of pork was further demonstrated by analyzing the physicochemical properties of the pork ( i.e. , weight loss rate, pH value, color index, and TVB-N value) and its protein profiles. Linalool did not completely kill S. flexneri in pork at minimum bactericidal concentration (MBC) concentration and its antibacterial effect of linalool was stronger during the initial stage of storage. During storage, linalool influenced the abundance of specific proteins in the pork, particularly those involved in pathways related to fat metabolism. These findings offer novel insights into the antibacterial efficacy of linalool and its underlying mechanism in pork.
研究领域:
食品科学
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微生物学
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分子生物学
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产品关联
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PubMed
高影响因子
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