CURRENT BIOLOGY
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2024
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IF 8.1
Yun Chen,Xia Hua,Siyu Li,Jiamei Zhao,Huan Yu,Dongyao Wang,Jiqiang Yang,Lijun Liu
Rice paddy has been the main source of anthropogenic methane (CH 4 ) emissions, with significant variations among rice varieties. 2-Acetyl-1-pyrroline (2-AP) is the key component of the pleasant aroma in fragrant rice. Here, we show that fragrant rice is metabolically active in nitrogen assimilation and exhibits high levels of 2-AP and that CH 4 fluxes at the booting stage and cumulative emissions are 25.5% and 14.8% lower, respectively, in fragrant rice paddies compared with nonfragrant rice paddies. Three precursors involved in 2-AP synthesis—proline, glutamic acid, and ornithine—are identified as crucial nitrogen compounds that significantly promote CH 4 oxidation in the rhizosphere. Augmenting 2-AP synthesis, either through foliar spraying or by utilizing CRISPR-Cas9 technology to generate knockout lines of BETAINE ALDEHYDE DEHYDROGENASE 2 gene, effectively enhances CH 4 oxidation and reduces CH 4 fluxes. Our findings reveal that the 2-AP metabolic pathway coordinates the carbon/nitrogen cycle to improve nitrogen assimilation along with high 2-AP levels and mitigate CH 4 emissions in paddy ecosystems.
研究领域:
植物学
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环境科学
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遗传学
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产品关联
DOI
PubMed
高影响因子
Journal of Soil Science and Plant Nutrition
·
2024
·Vol. 24
·期 1
·1301-1317
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IF 3.4
Mai Yiming,Ren Yong,Deng Siying,Ashraf Umair,Tang Xiangru,Duan Meiyang,Mo Zhaowen
Foliar application of ZnO Nanoparticles (ZnO-NPs) could reduce adverse effects of multiple abiotic stresses in crop plants. The present study was conducted to induce the low temperature stress tolerance in growing rice plants by the foliar application of ZnO-NPs. Two fragrant rice cultivars i.e., Meixiangzhan2 and Xiangyaxiangzhan were grown under normal temperature (NT, day/night: 28℃/25℃) and low temperature (LT, day/night: 15℃/12℃) after foliar application of Zn and ZnO-NPs with following treatments viz . , application of distilled water only (CK), ZnSO 4 ·7H 2 O at 6.6 mg·L −1 (CK1), and application of ZnO-NPs at 25, 50, 100, and 200 mg·L −1 regarded as N25, N50, N100, and N200, respectively. The growth and physiological attributes were examined to assess the potential of ZnO-NPs to induce low temperature stress tolerance in growing rice plants. The ZnO-NPs, and temperature × ZnO-NPs had a significant effect on the growth and physiological attributes ( p < 0.05) of rice seedlings. The ZnO-NPs application enhanced the root dry weight, root-shoot ratio, total dry weight, and chlorophyll a/b ratio in Xiangyaxiangzhan by 10.48%-18.10%, 1.96%-20.31%, 3.25%-6.02%, and 3.70%-6.11% at low temperature, respectively. The activity of antioxidant enzymes in Meixiangzhan 2 was increased, whilst the malondialdehyde (MDA) content in root was significantly declined. Moreover, lower levels of ZnO-NPs treatment regulated the activity of N-metabolizing enzymes i.e., nitrate reductase (NR), glutamate synthase (GS), glutamine oxoglutarate aminotransferase (GOGAT) and in seedlings under low temperature. Regarding correlation analysis, the N100 and N200 treatment exhibited a stronger correlation with growth-related attributes, compared to other treatments. Foliar application of ZnO-NPs at appropriate concentration (100 mg·L −1 ) could alleviate the inhibitory effect of low temperature stress on the early growth of fragrant rice.
研究领域:
植物科学
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纳米技术
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农业逆境管理
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）