Journal of Fungi
·
2024
·Vol. 10
·期 8
·526
·
IF 4.2
Qi Zeng,Lu Wang,Sha Long,Wanrong Dong,Yaoyao Li,Yuxin Chen,Gao Zhou
Botryosphaeria dothidea, a notorious plant pathogen, is responsible for causing chestnut rot during postharvest storage. This research aimed to assess the antifungal properties of perilla essential oil (PEO) and perillaldehyde (PAE) againstB. dothidea. PEO’s and PAE’s inhibitory effects onB. dothideawere investigated using an agar dilution method, a fumigation method, and an in vivo assay in chestnuts and shell buckets. Based on the results of gas chromatography-mass spectrometry, it was confirmed that the main component of PEO was elemicin. The antifungal mechanism of PEO and PAE againstB. dothideawas investigated by conducting staining experiments of the fungal cell wall and cell membrane. PEO and PAE strongly inhibit the mycelial growth ofB. dothideain a dose-dependent manner. The inhibitory mechanism is mainly related to the destruction of the integrity of the fungal cell wall and plasma membrane. Notably, PEO retains its antifungal efficacy againstB. dothideain chestnuts, effectively prolonging their storage life. These findings indicate that PEO and PAE are nontoxic, eco-friendly botanical fungicides, holding promise for controlling postharvest chestnut rot.
研究领域:
植物学
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真菌学
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食品科学
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DOI
PubMed
高影响因子
Foods
·
2020
·Vol. 9
·期 3
·345
·
IF 4.092
Jun-Yu Liang,Jie Xu,Ying-Ying Yang,Ya-Zhou Shao,Feng Zhou,Jun-Long Wang
Investigations have indicated that storage pests pose a great threat to global food security by damaging food crops and other food products derived from plants. Essential oils are proven to have significant effects on a large number of stored grain insects. This study evaluated the contact toxicity and fumigant activity of the essential oil extract from the aerial parts ofElsholtzia ciliataand its two major biochemical components against adults and larvae of the food storage pest beetleTribolium castaneum. Gas chromatography–mass spectrometry analysis revealed 16 different components derived from the essential oil ofE. ciliata, which included carvone (31.63%), limonene (22.05%), andα-caryophyllene (15.47%). Contact toxicity assay showed that the essential oil extract exhibited a microgram-level of killing activity againstT. castaneumadults (lethal dose 50 (LD50) = 7.79 μg/adult) and larvae (LD50= 24.87 μg/larva). Fumigant toxicity assay showed LD50of 11.61 mg/L air for adults and 8.73 mg/L air for larvae. Carvone and limonene also exhibited various levels of bioactivity. A binary mixture (2:6) of carvone and limonene displayed obvious contact toxicity againstT. castaneumadults (LD50= 10.84 μg/adult) and larvae (LD50= 30.62 μg/larva). Furthermore, carvone and limonene exhibited synergistic fumigant activity againstT. castaneumlarvae at a 1:7 ratio. Altogether, our results suggest thatE. ciliataessential oil and its two monomers have a potential application value to eliminateT. castaneum.Keywords:Elsholtzia ciliata;Tribolium castaneum;essential oil;carvone;limonene;insecticidal activity;synergistic effect
研究领域:
植物学
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昆虫学
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农业科学
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产品关联
DOI
PubMed
高影响因子
Genes
·
2022
·Vol. 13
·期 11
·1956
·
IF 4.141
Jie Chen,Xin Hao,Ruina Tan,Yang Li,Bowen Wang,Jialiang Pan,Wei Ma,Ling Ma
Bursaphelenchus xylophilus(PWN) causes pine wilt disease (PWD), which is one of the most devastating pine diseases worldwide. Cytochrome P450 (CYP) catalyzes the biosynthetic metabolism of terpenoids and plays an important role in the modification of secondary metabolites in all living organisms. We investigated the molecular characteristics and biological functions ofBx-cyp29A3inB. xylophilus. The bioinformatics analysis results indicated thatBx-cyp29A3has a transmembrane domain and could dock with L(−)-carvone. The gene expression pattern indicated thatBx-cyp29A3was expressed in 0.2, 0.4, 0.6, 0.8, and 1.0 mg/mL L(−)-carvone solutions. TheBx-cyp29A3expression increased in a dose-dependent manner and peaked at 24 h of exposure when the L(−)-carvone solution concentration was 0.8 mg/mL. However, the gene expression peaked at 0.6 mg/mL after 36 h. Furthermore, RNA interference (RNAi) indicated thatBx-cyp29A3played an essential role in the response to L(−)-carvone. The mortality rates of theBx-cyp29A3knockdown groups were higher than those of the control groups in the 0.4, 0.6, 0.8, and 1.0 mg/mL carvone solutions after 24 h of exposure or 36 h of exposure. In summary, bioinformatics provided the structural characteristics and conserved sequence properties ofBx-cyp29A3and its encoded protein, which provided a target gene for the study of the P450 family ofB. xylophilus. Gene silencing experiments clarified the function ofBx-cyp29A3in the immune defense ofB. xylophilus.This study provides a basis for the screening of new molecular targets for the prevention and management ofB. xylophilus.Keywords:cytochrome P450;L(−)-carvone;Bursaphelenchus xylophilus;RNAi
研究领域:
分子生物学
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植物病理学
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线虫学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 15
·2645
·
IF 5.1
Cong Zhang,Kai Jiang,Yilin Lin,Rui Cui,Hong Wu
In this study, a composite film with dual antioxidant and antibacterial properties was prepared by combining 2% chitosan and 7% gelatin (2:1, w:w), with D-carvone (0–4%) as the primary active component. The effect of D-carvone content on the performance of the composite films was systematically investigated. The results showed that adding 1% D-carvone increased the water contact angle by 28%, increased the elongation at break by 35%, and decreased the WVTR by 18%. FTIR and SEM confirmed that ≤2% D-carvone uniformly bonded with the substrate through hydrogen bonds, and the film was dense and non-porous. In addition, the DPPH scavenging rate of the 1–2% D-carvone composite film increased to about 30–40%, and the ABTS+scavenging rate increased to about 35–40%; the antibacterial effect on Escherichia coli and Staphylococcus aureus increased by more than 70%. However, when the addition amount was too high (exceeding 2%), the composite film became agglomerated, microporous, and phase-separated, affecting the film performance, and due to its own taste, it reduced the sensory quality of the noodles. Comprehensively, the composites showed better performance when the content of D-carvone was 1–2% and also the best effect for freshness preservation in Xinjiang ramen. This study provides a broad application prospect for natural terpene compound-based composite films in the field of high-moisture, multi-fat food preservation, and provides a theoretical basis and practical guidance for the development of efficient and safe food packaging materials. In the future, the composite film can be further optimized, and the effect of flavor can be further explored to meet the needs of different food preservation methods.
研究领域:
食品科学
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高分子科学
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材料工程
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食品包装
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抗菌技术
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抗氧化研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INVERTEBRATE PATHOLOGY
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2024
·
IF 3.6
Peng-hua Bai,Jin-Ping Yu,Rui-rui Hu,Qian-wen Fu,Hai-chao Wu,Xing-yue Li,Guo-hao Zu,Bao-sheng Liu,Yu Zhang
Entomopathogenic nematodes (EPNs) use the chemical cues emitted by insects and insect-damaged plants to locate their hosts. Steinernema carpocapsae , a species of EPN, is an established biocontrol agent used against insect pests. Despite its promising potential, the molecular mechanisms underlying its ability to detect plant volatiles remain poorly understood. In this study, we investigated the response of S. carpocapsae infective juveniles (IJs) to 8 different plant volatiles. Among these, carvone was found to be the most attractive volatile compound. To understand the molecular basis of the response of IJs to carvone, we used RNA-Seq technology to identify gene expression changes in response to carvone treatment. Transcriptome analysis revealed 721 differentially expressed genes (DEGs) between carvone-treated and control groups, with 403 genes being significantly upregulated and 318 genes downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the responsive DEGs to carvone attraction were mainly involved in locomotion, localization, behavior, response to stimulus, and olfactory transduction. We also identified four upregulated genes of chemoreceptor and response to stimulus that were involved in the response of IJs to carvone attraction. Our results provide insights into the potential transcriptional mechanisms underlying the response of S. carpocapsae to carvone, which can be utilized to develop environmentally friendly strategies for attracting EPNs.
研究领域:
昆虫学
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分子生物学
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植物科学
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生物防治
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产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2023
·Vol. 10
·期 12
·3317-3328
·
IF 7.3
Baoyu Li,Wengui Duan,Guishan Lin,Yucheng Cui,Rongzhu Wen,Chuwen Liu,Yulu Xie
Natural product-based nanopesticide is an effective and sustainable approach for solving the environmental issues caused by the application of current commercial herbicides. In this study, 21 novel L-carvone-derived 4-methyl-1,2,4-triazole-thioether derivatives 5a–5u were rationally designed by transketolase-based in silicon virtual screening, synthesized, and structurally characterized using FT-IR spectroscopy, 1H/13C NMR spectroscopy, and HRMS technique. Besides, the herbicidal activity of the target compounds against Brassica campestris and Echinochloa crusgalli L. was evaluated, and the bioassay results revealed that compound 5a with the most significant herbicidal and TK-inhibition activities could serve as a potential TK inhibitor and could serve as a leading compound for further study of green herbicides. Subsequently, the molecular skeletons of chitosan and L-carvone were incorporated for developing high-efficiency nanopesticide carriers, and thus samples 7a–7d were synthesized by N-alkylation of chitosan and characterized using various methods. Compared with unmodified chitosan, L-carvone-derived nanochitosan carrier 7b with the micro-morphologies of nubby lumps and porous surfaces exhibited higher dispersibility and similar thermostability. Finally, L-carvone-derived 4-methyl-1,2,4-triazole-thioether/nanochitosan complexes 7b/5a, 7c/5a, and 7d/5a with better 5a-loading capacities and multi-stage sustained releasing performances were constructed, and the herbicidal activities of 7b/5a and 7d/5a against Brassica campestris were evaluated in the petri dish and greenhouse. It was found that 7b/5a and 7d/5a exhibited significant herbicidal activities. Among them, 7b/5a had great potential as a green and eco-friendly nanopesticide for weed control.
研究领域:
植物学
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农业科学
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材料科学
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DOI
高影响因子
Molecular Catalysis
·
2024
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IF 3.9
Zehao Li,Wanying Wang,Guoguo Wang,Yingjie Feng,Yan Chen,Zhijian Li,Lulu Zhang,Jinchu Yang
Carvone has been widely used in pharmaceutical, food, cosmetic, and chemical industries due to its pleasant odor with high economic value and pharmacological activities. Carveol dehydrogenase is an enzyme that catalyzes the oxidation of carveol into carvone in the presence of NAD(P) + . In this study, two dehydrogenase genes from Klebsiella sp. O852 ( KlebADH1 and KlebADH2 ) were cloned and expressed in Escherichia coli BL21(DE3), and the purified enzymes were characterized for their biocatalytic properties. KlebADH1 and KlebADH2 displayed optimal catalytic activity at 40 °C and 30 °C, respectively, at pH 9. In addition to carveol, KlebADH1 had the potential to catalyze the oxidation of perillyl alcohol, nerol and geraniol, while borneol and geraniol could serve as potential substrates for KlebADH2. The substrate kinetic parameters of enzymes were performed by changing the concentration of carveol, and the results indicated that KlebADH2 exhibited a high affinity for carveol. Additionally, KlebADH1 and KlebADH2 were confirmed to possess carveol dehydrogenase activity through a double-enzyme-coupled (DEC) biotransformation system. The investigation of the biocatalytic properties of KlebADH1 and KlebADH2 laid a solid foundation for further study of carvone sustainable synthesis.
研究领域:
生物技术
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酶学
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化学工程
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药学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SAFETY
·
2023
·
IF 2.4
Shengjie Li,Feng Cheng,Xueqin Cao,Bakri Mahinur,Maitinuer Maiwulanjiang
As the interest in “natural” and “safe” products grows, the use of natural products instead of synthetic preservatives to combat food spoilage and poisoning caused by microorganisms during processing and storage has become a prioritized option. The present research evaluated the antibacterial activity of the Mentha longifolia L. essential oil (MLEO) against several pathogenic bacteria, and the mechanism of action against methicillin-resistant Staphylococcus aureus (MRSA). Gas Chromatography Quadrupole Time-of-Flight Mass Spectrometry (GC-Q-TOF MS) analysis suggested that main components of MLEO were carvone (47.39%) and limonene (12.48%). The oil showed considerable antibacterial activity with MIC values of 0.394–1.576 mg/mL, and could be a promising bactericide. Non-targeted metabolomics analysis based on GC-Q-TOF MS identified 66 different metabolites, and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of these metabolites revealed that MLEO achieves the effects by affecting amino acid metabolism in MRSA.
研究领域:
微生物学
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药理学
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食品科学
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分析化学
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产品关联
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2022
·Vol. 70
·期 2
·697-706
·
IF 2.724
Shijin Wu,Bijiao Wang,Hongde Yan
Asymmetric reduction of ( R )-carvone and ketoisophorone by an engineered ene-reductase from Galdieria sulphuraria (GsOYE) combined with glucose dehydrogenase for NADPH regeneration were studied. A semi-rational protein engineering was used to enhance the activity and selectivity of GsOYE. Upon the sequence alignment and molecular docking results, two amino acid residues at positions 66 and 270 were selected as saturation mutation sites. Finally, a single substitution variant of GsOYE-N270A with complete conversion (100%) and diastereoselectivity (de p >99%) for reduction of ( R )-carvone and a double substitution variant GsOYE-Y66P/N270H with improved stereoselectivity for reduction of ketoisophorone were obtained.
研究领域:
生物化学
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蛋白质工程
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催化
·
酶学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shijin Wu,Xiaojing Ma,Hongde Yan
The asymmetric reduction of α , β -unsaturated compounds conjugated with electron-withdrawing group by ene-reductases (ERs) is a valuable method for the synthesis of enantiopure chiral compounds. This study introduced an ER from Corynebacterium casei ( Cc ER) which was heterologously expressed in Escherichia coli BL21(DE3), and the purified recombinant Cc ER was characterized for its biocatalytic properties. Cc ER exhibited the highest specific activity at 40 °C and pH 6.5, and showcased appreciable stability below 40 °C over a pH range of 6.0–7.0. The enzyme displayed high resistance to methanol. Cc ER accepted NADH or NADPH as a cofactor and exhibited a broad substrate spectrum towards α , β -unsaturated compounds. It achieved complete conversion of 2-cyclohexen-1-one and good performance for stereoselective reduction of ( R )-carvone (conversion 98 %, diastereoselectivity 96 %). This study highlights the robustness and potential of Cc ER.
研究领域:
生物化学
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生物技术
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酶学
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有机化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2024
·
IF 10.7
Beitao Liu,Jiahui Li,Cijian Zhang,Yibo Wang,Xigao Jian,Zhihuan Weng
Environmental economics is intensifying the necessity for the advancement of recycling technologies to address the increasing volume of discarded thermoset polymers. Covalent adaptable networks (CANs) provide a new strategy for the recycling and functionalization of thermosetting resins. Herein, a carvone-based CAN is developed, in which thermally driven depolymerization of mechanically robust polymer networks is achieved by dynamic thiol-Michael bonds. The original cross-linking network can be retained through simple drying and hot-pressing steps without purifying degradation products. The reversibility of the thiol-Michael group is demonstrated and the CAN exhibits shape memory behaviors triggered by human body temperature. We propose a micro-robotic injection model with good mechanical strength and fast response speed for complex 1D to 3D shape morphing in aqueous media at 37 °C. Our findings present a high-quality strategy to realize recycling and functionalization of bio-based thermoset resins and expand the toolbox for the preparation of bio-based CANs with thermo-responsive behavior.
研究领域:
材料科学
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聚合物化学
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可持续材料
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回收技术
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产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Qi Lu,Qiong Wang,Yi Wang,Peng Guo,De Yang,Shujing Xue
This study investigated the effects of gamma irradiation (GI) at a dose of 0.6 kGy on the dynamic changes of volatile and non-volatile compounds in Cara Cara oranges during postharvest storage. Sixty-one volatile components were identified, with total relative concentrations ranging from 2465.89 to 5785.54 μg/L. Limonene, linalool, ethyl butyrate, ethyl 2-methylbutyrate and nerolidol were identified as key aroma-active compounds in fresh Cara Cara oranges. The application of GI treatment reduced limonene content and enhanced esterification, promoting ethyl butyrate and ethyl 2-methylbutyrate as dominant aroma contributors in stored fruit. The GI treatment significantly reduced the fruit damage rate and MDA content while enhancing antioxidant capacity in Cara Cara fruit during prolonged storage. Through a widely targeted analysis, 134 differential non-volatile metabolites were annotated in the KEGG pathway. Elevated levels of key metabolites and the increased activity of rate-limiting enzymes (PFK, PAL, 4CL, ACC, SDH, and MDH), suggested GI might potentially accelerate metabolic flux in postharvest Cara Cara oranges. Overall, this study indicated that GI treatment could effectively reduce postharvest damage in Cara Cara oranges while modulating flavor profiles and metabolic responses.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）