FOOD CHEMISTRY
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2024
·
IF 8.5
Can Zhang,Yongfei Zhou,Li Ming,Lihong Chen,Minqiao Xue,Jing Zhang,Hongyan Zhang
The inclusion of artificial food additives such as vanillin in infant formula should be strictly monitored to mitigate potential negative impacts on the dietary habits and health of infants. This raises a necessity of an accurate inspection and prompt feedback of vanillin in infant foods. In this study, colorimetric and fluorescent dual–mode assays based on CuNS/Fe 3 O 4 @MIPs were established to detect vanillin selectively and sensitively. Quantification of vanillin could be achieved with linear detection ranges of 1–100 μM and 1–150 μM for the colorimetric and fluorescent assays respectively. The corresponding detection limits were 0.11 and 0.10 μM respectively. The CuNS/Fe 3 O 4 @MIPs–based dual–mode assays exhibited good selectivity and stability for vanillin detection in infant formula and milk-based foods. Hence, this method can serve as a reliable tool for the cost-saving, effective and quantitative determination of vanillin in infant foods, with the potential to replace conventional instrumental analysis.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Mengying Wang,Yuepeng Lu,Yong Yang,Jiahao Yu,Yechao Chen,Fengqin Tu,Jing Hou,Zong Yang,Xiaoming Jiang
Recently, we found that vanillin commonly exists in sesame oils, while vanillin compounds are not allowed to be used as food additives in edible vegetable oils according to Chinese standards for Food Additives (GB2760-2014). To trace the source of vanillin in sesame oil, an accurate and reliable method was developed and validated for the determination of vanillin, methyl vanillin and ethyl vanillin in sesame samples based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The limit of determination (LOD) and limit of quantification (LOQ) of the established method were in the range of 17.4–33.1 and 52.2–99.2 μg kg −1 , respectively. The calibration curves of the three vanillin compounds exhibited good linearity between 0 and 400 ng mL −1 , with a high correlation coefficient greater than 0.999. The recoveries ranged from 91.7% to 112%, with a low relative standard deviation (RSD) less than 5.55%. Then this method was applied for the detection of sesame seed and intermediate samples. The results showed that all samples contained vanillin in a concentration range of 382–3804 μg kg −1 , while methyl vanillin and ethyl vanillin were not detected in those samples. Hence, the vanillin in sesame oil may originate from its raw materials, indicating that vanillin was not a good detection marker for illegal addition of flavor enhancers in sesame oils.
研究领域:
分析化学
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食品安全
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食品科学
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产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2020
·Vol. 22
·期 4
·1275-1290
·
IF 9.48
Binbo Wang,Songqi Ma,Qiong Li,Hua Zhang,Junjie Liu,Rong Wang,Zhiquan Chen,Xiwei Xu,Sheng Wang,Na Lu,Yanlin Liu,Shifeng Yan,Jin Zhu
Plastics are indispensable in modern society, but are non-sustainable resources, releasing hazardous chemicals during their service life, and post-disposal issues make traditional plastics a risk. Herein, we report a “digestible”, rigid-and-flexible, bio-sourced building block for high-performance degradable plastics. This building block was synthesized from the bioresources vanillin (lignin derivative) and glycerol through solvent-free acetalization with a high conversion rate and high selectivity. It could be extremely rapidly degraded into non-toxic vanillin and glycerol under mild acidic conditions even at a similar pH and temperature to gastric juice in the human stomach (“digested”), resulting in the outstanding chemical degradability of its corresponding epoxy thermosets, which is beneficial for their recycling. By virtue of the benzene ring, heterocycle, and methoxyl group-related hydrogen bond, the degradable thermosetting plastic showed much higher mechanical properties (stronger and tougher) and comparable thermal properties relative to a commercial high-performance counterpart based on bisphenol A (BPA). This favorable performance combination has never been reported for plastics. Thus, this bio-derived building block exhibits great potential as a sustainable and upgraded alternative to petroleum-sourced aromatic chemicals such as BPA for high-performance plastics.
研究领域:
材料科学
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化学工程
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环境科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Xiaochong Zhu,Jieyuan Wu,Shizhong Li,La Xiang,Jian-Ming Jin,Chaoning Liang,Shuang-Yan Tang
Eugenol, the main component of essential oil from the Syzygium aromaticum clove tree, has great potential as an alternative bioresource feedstock for biosynthesis purposes. Although eugenol degradation to ferulic acid was investigated, an efficient method for directly converting eugenol to targeted natural products has not been established. Herein we identified the inherent inhibitions by simply combining the previously reported ferulic acid biosynthetic pathway and vanillin biosynthetic pathway. To overcome this, we developed a novel biosynthetic pathway for converting eugenol into vanillin, by introducing cinnamoyl-CoA reductase (CCR), which catalyzes conversion of coniferyl aldehyde to feruloyl-CoA. This approach bypasses the need for two catalysts, namely coniferyl aldehyde dehydrogenase and feruloyl-CoA synthetase, thereby eliminating inhibition while simplifying the pathway. To further improve efficiency, we enhanced CCR catalytic efficiency via directed evolution and leveraged an artificialvanillin biosensor for high-throughput screening. Switching the cofactor preference of CCR from NADP+ to NAD+ significantly improved pathway efficiency. This newly designed pathway provides an alternative strategy for efficiently biosynthesizing feruloyl-CoA-derived natural products using eugenol.
研究领域:
生物化学
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合成生物学
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产品关联
DOI
PubMed
高影响因子
HOLZFORSCHUNG
·
2023
·Vol. 77
·期 7
·527-540
·
IF 2.4
Li Yan,Zeyao Yan,Jiang Chen,Zhangjing Chen,Yafang Lei
Vanillin is an antifungal and environmentally friendly compound. In this study, vanillin and silica microcapsules (VSM) were microencapsulated using the sol-gel method and then impregnated into wood. Scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDXA) and transmission electron microscopy (TEM) were used to characterize the morphological structure and distribution of VSM in wood. Fourier transform infrared spectroscopy (FTIR) was used to study the intermolecular interactions between VSM and wood. The antifungal performance of the VSM-treated wood was evaluated. The study revealed that VSM had good sustained-release performance and decay resistance. Mass losses of VSM-treated wood after leaching and exposure to Trametes versicolor (L.) Quel. and Gloephyllum trabeum (Pers.) Murrill decreased from mass losses of 20.8 % and 15.9 % of the control group to 9.2 % and 6.4 %, respectively. VSM treatment disrupted the mycelium of T. versicolor and G. trabeum , inhibited their respiratory metabolism, and the ligninase-laccase enzyme activity of T. versicolor . Meanwhile, MOR and MOE of VSM-treated wood were 96.7 MPa and 12.3 GPa which were 28.8 % and 11.5 % higher than the control group, respectively.
研究领域:
材料科学
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生物学
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DOI
高影响因子
Microbial Cell Factories
·
2025
·Vol. 24
·期 1
·1-12
·
IF 4.9
Tian Yongqing,Yang Yige,Ni Minmin,Wo Jing
Background Vanillin is a widely utilized flavor compound of significant value in the food and pharmaceutical sectors, which can be obtained through natural extraction, chemical synthesis, or biotechnological processes. However, the yield from vanilla pods is insufficient to meet market demand, and chemically synthesized vanillin not only encounters limitations in its application within the food and pharmaceutical industries but also needs to address environmental concerns and unsustainable raw material sources. Hence, it is imperative to explore alternative approaches to develop an efficient and cost-effective green vanillin. To address the challenges encountered in vanillin biosynthesis, such as substrate uptake limitations and product-induced inhibition of cell growth,we leveraged the advantages of surface display technology and artificial multi-enzyme scaffolds to construct a hybrid surface-display biocatalytic system by assembling Eugenol oxidase (EUGO) and dioxygenase (NOV1), which can convert lignin biowaste 4- n -propylguaiacol (4-PG) into vanillin on the surface of Escherichia coli BL21(DE3). Results To assemble bioactive macromolecules of EUGO and NOV1 on the surface of E. coli BL21(DE3), we utilized Lpp-OmpA-SpyCatcher (LOAS) as an anchoring motif and displayed EUGO-linker-NOV1-SpyTag (ELNS) by covalent interaction between SpyTag andSpyCatcher to allow their spatial proximity. After optimization of the reaction system, our self-assembly display system exhibited highly efficiency in converting 4-PG into vanillin and reached a final concentration of vanillin at 12.58 g/L, 2.5 times higher than that achieved by thewhole-cell biocatalytic system. The LOAS-ELNS display system was applied to the sustainable biosynthesis of vanillin from lignin-derived 4-n-propylguaiacol at least 10 times. Conclusions This work provided a generalized approach to co-expressing proteins and offered an efficient, eco-friendly, and renewable method for the biosynthesis of vanillin from 4-PG.
研究领域:
生物化学
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合成生物学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 138
·期 25
·50588
·
IF 3.125
Zongwei Guo,Zice Xu,Zhiqiang Dong,Mengjie Zhang,Zhiyuan Chi,Ming Li,Lei Shang,Yuhui Ao
Vanillin, a rigid compound can be separated from lignin, is a promising sustainable candidate for industrial and high performance polymers, while synthesis of hexa-epoxies is challenging. Meanwhile, carbon fiber reinforced bio-based polymers combining high performance are more difficult to be achieved because of the contradictions of liquidity and high rigidity in the polymer structure and performance. In this paper, a novel hexa-epoxy functionalized bio-based epoxy resin (HPVIGEP) with a multi-arm star structure, which simultaneously reduced the viscosity and improved thermo-mechanical properties. The rheological behavior analysis results of HPVIGEP indicated that the viscosity was 3406.9 mPa·s at 25°C, which dramatically decreased by 75.8% compared to DGEBA (14,096 mPa·s), leading to excellent processability and adaptability. At the same time, the study on mechanical properties revealed that the cured HPVIGEP manifested 30.6%, 33.7% and 49.0% in higher tensile strength, tensile modulus and storage modulus (30°C) than of cured commercial epoxy, respectively. The tensile strength and flexural strength of carbon fiber composites which were applied HPVIGEP were increased by 9.3% and 10.9%, respectively. In a word, this work provides the promise for the application of environmentally friendly bio-based composite materials.
研究领域:
材料科学
·
可持续化学
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产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 193
·期 4
·1116-1128
·
IF 2.926
Wang Qian,Wu Xiaomei,Lu Xinyi,He Yucai,Ma Baodi,Xu Yi
Currently, the biotechnological preparation of fragrances using natural materials attracted growing attention. Enzymatic synthesis of vanillin from isoeugenol by recombinant isoeugenol monooxygenase from Pseudomonas nitroreducens Jin1 was systematically investigated herein. With series of work on the construction of recombinant E. coli over-expressing isoeugenol monooxygenase, optimization of the culture conditions for enzyme production and reaction process for converting isoeugenol into vanillin, an increase of 22-fold in the enzyme activity (2050 U/L) was obtained, and the conversion was significantly increased at high substrate concentration with the aid of magnetic chitosan membrane for product isolation in situ. Under optimal conditions, the product concentration and space-time yield reached 252 mM and 115 g/L/d, respectively, and vanillin was obtained in 82.3% yield and > 99% purity in the gram preparative scale. The developed bioprocess showed application potential for efficient preparation of vanillin from inexpensive natural resources.
研究领域:
生物技术
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酶学
·
天然产物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2018
·Vol. 7
·期 3
·1233-1243
·
IF 9.931
Songqi Ma,Jingjing Wei,Zhen Jia,Tao Yu,Wangchao Yuan,Qiong Li,Sheng Wang,Shusen You,Ren Liu,Jin Zhu
Conventional thermosets, widely used in composites, coatings, electronic packing materials, etc., are typically made of non-sustainable fossil resources and cannot be recycled under mild conditions. Degradable thermosets are promising alternatives, while combining ready degradability and high performance is a challenge. In this paper, for the first time, a spiro diacetal structure was utilized to synthesize readily recyclable thermosetting materials with high performance. A renewable bioresource, lignin derived vanillin, was used to produce a rigid spiro diacetal trigger and further built into three thermosets. Among them, spiro diacetal epoxy resin was systematically investigated and was proved to be readily degradable under mild acidic conditions while maintaining stability under neutral or basic conditions and showing outstanding thermal stability. Meanwhile, its glass transition temperature, tensile strength, modulus, elongation at break are comparable to or even better than those of conventional bisphenol A epoxy resin. Moreover, facilely recyclable carbon fiber reinforced composites and convenient-removal coatings were achieved from this epoxy resin accompanied by excellent performances.
研究领域:
材料科学
·
化学工程
·
生物质利用
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产品关联
DOI
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2021
·
IF 3.575
Likun Yan,Xiao Zhang,Jie Fu,Qiang Liu,Xiaohua Lei,Zhenyu Cao,Ju Zhang,Yaoli Shao,Qing Tong,Wei Qin,Xinxu Liu,Chun Liu,Zhiqiang Liu,Zhenghao Li,Jueliang Lu,Xundi Xu
Transient receptor potential vanilloid 3 (TRPV3) is a member of the TRP superfamily. Previous studies have demonstrated that TRPV3 is associated with myocardial fibrosis. However, the role of TRPV3 in hepatic fibrosis and its underlying mechanisms are still unclear. This study aimed to elucidate the underlying effects of TRPV3 on hepatic fibrosis at multiple biological levels. First, immunohistochemical staining was performed to examine TRPV3 expression in human hepatic cirrhosis tissues. Then, we established a CCl 4 -induced hepatic fibrosis mouse model. The TRPV3 selective agonist drofenine and its inhibitor, forsythoside B, were intraperitoneally injected to investigate the relationship between TRPV3 and liver fibrosis progression. Finally, in vitro studies were performed using hepatic stellate cells (HSCs) to discover the potential molecular biological mechanisms. Immunohistochemistry revealed TRPV3 overexpression in liver cirrhosis. In the liver fibrosis groups, TRPV3 inhibitor treatment significantly reduced liver fibrosis, while TRPV3 agonist exacerbated its progression. In HSCs, knocking down TRPV3 with siRNA impaired DNA synthesis and cell proliferation and increased cell apoptosis. Furthermore, we found that knockdown of TRPV3 could reduce the lectin like oxidized lowdensity lipoprotein receptor-1 (LOX-1) protein levels. Our research suggests that lower expression or functional levels of TRPV3 can ameliorate the inflammatory response and fibrotic tissue proliferation.
研究领域:
药理学
·
肝病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）