Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Wenju Liu,Bin Li,Shikun Wu,Shaofeng Wang,Miaomiao He,Hongjuan Bai,Han Wang,Yanmin Shen
Coumaric acid (p-CA), a naturally occurring antimicrobial agent with a broad range of potential applications, is severely limited by its poor water solubility. This study addressed this challenge by encapsulating p-CA within a biocompatible cyclodextrin metal-organic framework (CD-MOF), achieving a maximum drug-loading capacity of 10.217 ± 0.303 %. We performed comprehensive characterization involving X-ray diffraction (XRD), Fourier Transform infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Thermogravimetry Analysis (TGA) and Brunauer Emmett Teller (BET) was undertaken to confirm the successful formation of the inclusion complex. It is noteworthy that the water solubility of p-CA at 25 °C increased 10-fold upon encapsulation, with rapid release achieved within 30 min. Encapsulated p-CA showed enhanced oxidation resistance (increased from 81.527 ± 0.871 % to 85.222 ± 0.871 %), as evidenced by DPPH-scavenging assays, and retained the antimicrobial activity of p-CA (Both p-CA and p-CA/CD-MOF exhibited 100 % antibacterial efficacy against Staphylococcus aureus and approximately 90 % efficacy against Escherichia coli ). Molecular docking and dynamics simulations revealed that p-CA inhibited bacterial glucose transport by occupying the active site of enzyme IIC (EIIC). Furthermore, experiments on the preservation of freshness in strawberries demonstrated the efficacy of p-CA/CD-MOF in maintaining fruit quality over 5 days at 25 °C. These findings emphasize the potential of CD-MOF encapsulation to circumvent the solubility limitations of p-CA, thereby facilitating its expanded use in food preservation and biomedical applications.
研究领域:
食品科学
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材料科学
·
生物医学工程
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药物递送
·
抗菌剂研究
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Lu Ping,Ji Xuemeng,Xue Juan,Dong Yinping,Chen Xi
Screening drugs and compounds to fight against Cronobacter sakazakii (C. sakazakii), one of the most common pathogens that can cause fatal necrotizing enterocolitis, septicema and meningitis, is still needed. We found that p-coumaric acid (pCA) has an inhibitory effect on C. sakazakii in vitro and in vivo. Proteomic changes of C. sakazakii BAA-894 exposed to pCA were studied to reveal the antibacterial mechanisms involved. A total of 1,553 proteins were identified in C. sakazakii BAA-894 by label-free proteomics analysis. Fuzzy cluster analysis showed that 33 were up-regulated, and 110 were down-regulated with pCA treatment. Gene Ontology (GO) analysis concluded that pCA caused the change of metabolic state of bacteria and generally in the state of metabolic inhibition. KEGG Enrichment Analysis (KEGG) analysis showed that pCA inhibited energy metabolism and distorted the balance of amino acid metabolism. Posttranslational modification analysis showed that pCA affected the deamidation of three proteins, including Elongation factor Tu, one of the vital proteins in bacteria. Molecular docking suggested the hydrogen bond between the pCA carboxyl group and Elongation factor Tu Asn-64 might contribute to deamidation. Overall, we found that pCA interfered with cellular energy and amino acid metabolism and promoted elongation factor Tu deamidation, suggesting that pCA can be an effective natural substitute to control C. sakazakii.
研究领域:
微生物学
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蛋白质组学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Yong Hou,Tonghe Liu,Zhonghao Sun,Xudong Xu,Zhaocui Sun,Zongyang Li,Jianzhou Liu,Sichao Tian,Yihang Li,Nailiang Zhu,Haitao Liu,Guoxu Ma
Pancreatic cancer is one of the most devastating solid tumors and lacks ideal therapeutic options. The efficiency of using classic chemotherapeutic agents like gemcitabine (GEM) solely has been limited immensely owing to severe resistance and systemic toxicity, thus necessitating alternative multi-pronged strategies. Multicomponent supramolecular co-assembly leveraging natural small molecules (NSMs) cannot only produce carrier-free hydrogel materials having superior mechanical properties for convenient administration, but also achieve optimal synergies in combinational drugs therapy. Herein, a natural structural analog of GEM with favorable pro-apoptotic effect, adenosine (Ado), was found firstly to self-assemble spontaneously into a hydrogel (Ado-gel). Sequentially, we constructed a novel two-component hydrogel (DA-gel) via co-assembling Ado with another NSM evidenced here as new autophagy inhibitor, gentisic acid (2,5-dihydroxybenzoic acid, DHB). Addition of DHB had reinforced the thermal-responsiveness and viscoelasticity evidently in DA-gel, which ascribed to the heightened intermolecular hydrogen bonding and π-π stacking interactions. Together with its good injectability and biosafety, the local injection can be conducted smoothly so as to improve the compliableness of patients in clinical settings. More intriguingly, DA-gel was proven to exhibit the best anti-tumor efficiency in pancreatic carcinoma comparing with corresponding single-component and mixture in vitro and vivo , possibly due to its unique supramolecular structure amplifying two-component synergistic effects on inhibition of autophagy and mitochondrial injury, thus lead to more obvious apoptosis. Overall, the presented co-assembling strategy exhibits admirable synergistic effects, thereby may provide a new perspective for functional application of multiple NSMs and a promising alternative to current chemotherapy for the treatment of pancreatic cancer.
研究领域:
肿瘤学
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药剂学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Xuemei Meng,Tong Zhang,Jun Li,Xiya Cao,Linan Duan,Haitian Fang,Junnan Xu
To overcome the poor solubility and bioavailability of p -coumaric acid ( p -CA), an inclusion complex ( p -CA- β -CD) of p -CA and β -cyclodextrin ( β -CD) was prepared. FT-IR, XRD, DSC, and SEM were performed to characterize the p -CA- β -CD complex. Additionally, the antibacterial activity of p -CA- β -CD against Alicyclobacillus acidoterrestris and the underlying mechanisms were investigated. The results confirmed the successful fabrication of p -CA- β -CD and demonstrated a significant enhancement in p -CA solubility upon complexation with β -CD. p -CA- β -CD effectively inhibited A. acidoterrestris , with an MIC of 1.4 mg/mL, by damaging cell membrane, reducing intracellular pH and ATP, increasing ROS levels, and altering cell morphology. Moreover, 1MIC of p -CA- β -CD caused 2.89 and 2.18 log reductions of A. acidoterrestris during apple juice storage at 4 °C and 25 °C, respectively, without remarkable effect on physicochemical indexes and sensory quality. These findings suggested that p -CA- β -CD could potentially be an emerging and promising natural agent for controlling A. acidoterrestris in juice.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Wanyi Zhou,Ruosi Fang,Qihe Chen
It was studied that gallic and protocatechuic acids played important roles in ethyl carbamate (EC) forming. Gallic and protocatechuic acids can reduce the arginine consumption through inhibiting the arginine deiminase enzyme. Therefore, they are generally added to regulate EC catabolism in the course of yellow rice wine leavening at the third day. In this work, gallic and protocatechuic acids made little influence on the growth of Saccharomyces cerevisiae . Besides, the addition of 200 mg/L gallic or protocatechuic acid could prevent the transformation from urea/citrulline to EC. Gallic acid showed better inhibiting effect that the content of EC could be reduced by 91.9% at most. Furthermore, the production of amino acids and volatile flavor compounds are not markedly affected by phenolic compounds. The discoveries reveal that EC can be reduced by supplying gallic acid or protocatechuic acid while yellow rice wine leavening.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Anti-Cancer Agents in Medicinal Chemistry
·
2023
·Vol. 23
·期 13
·1577-1586
·
IF 2.8
Bo Yang,Bowen Wang,Guoliang Wang,Wenliang Cao,Qian Wang,Hao Pu,Wei An
Background: P-coumaric acid (p-CA) is a kind of phenylpropionic acid derived from aromatic amino acids, which is widely distributed in many plants and human diets. It has strong pharmacological and inhibitory effects on a variety of tumors. However, the role of p-CA in osteosarcoma, a tumor with a poor prognosis, is still unknown. Therefore, we aimed to evaluate the effect of p-CA on osteosarcoma and explore its potential mechanism. Objective: This study aimed to investigate whether p-CA has an inhibitory effect on the growth of osteosarcoma cells and explore its potential mechanism. Methods: MTT assay and clonogenic assay were used to detect the effect of p-CA on the proliferation of osteosarcoma cells. The effect of p-CA on apoptosis of osteosarcoma cells was detected by the Hoechst staining and flow cytometry. The effects of p-CA on the migration and invasion of osteosarcoma cells were detected by scratch healing assay and Transwell invasion assay. Western blot and PI3K/Akt pathway activator 740Y-P were used to detect the anti-tumor mechanism of p-CA on osteosarcoma cells. The effect of p-CA on osteosarcoma cells in vivo was verified by an orthotopic osteosarcoma tumor animal model in nude mice. Results: MTT assay and clonogenic assay showed that p-CA inhibited the proliferation of osteosarcoma cells. Hoechst stain and flow cytometry showed that p-CA could induce apoptosis of osteosarcoma cells and lead to G2 phase arrest of osteosarcoma cells. Transwell assay and scratch healing assay found that p-CA could inhibit the migration and invasion of osteosarcoma cells. Western blot showed that p-CA could inhibit the activity of the PI3K/Akt signaling pathway in osteosarcoma cells, and 740Y-P could reverse its inhibitory effect. In vivo mouse models, p-CA has an antitumor effect on osteosarcoma cells in vivo, and at the same time, it has less toxic side effects on mice. Conclusion: This study demonstrated that p-CA could effectively inhibit the proliferation, migration and invasion of osteosarcoma cells and promote apoptosis. p-CA may play an anti-osteosarcoma role by inhibiting PI3K/Akt signaling pathway.
研究领域:
肿瘤学
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药理学
·
分子生物学
✓ 已验证
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Jiangmei Zheng,Ruobin Sun,Dan Wu,Pengcheng Chen,Pu Zheng
Phenylalanine ammonia-lyase (PAL) plays a pivotal role in the biosynthesis of phenylalanine. PAL from Zea mays (ZmPAL2) exhibits a bi-function of direct deamination of L-phenylalanine (L-Phe) or L-tyrosine(-L-Tyr) to form trans -cinnamic acid or p -coumaric acid. trans -Cinnamic acid and p -coumaric acid are mainly used in flavors and fragrances, food additives, pharmaceutical and other fields. Here, the Activity of ZmPAL2 toward L-Phe or L-Tyr was improved by using semi-rational and rational designs. The catalytic efficiency ( k cat / K m ) of mutant PT10 (V258I/I459V/Q484N) against L-Phe was 30.8 μM −1 s −1 , a 4.5-fold increase compared to the parent, and the catalytic efficiency of mutant PA1 (F135H/I459L) to L-tyrosine exhibited 8.6 μM −1 s −1 , which was 1.6-fold of the parent. The yield of trans -cinnamic acid in PT10 reached 30.75 g/L with a conversion rate of 98%. Meanwhile, PA1 converted L-Tyr to yield 3.12 g/L of p -coumaric acid with a conversion rate of 95%. Suggesting these two engineered ZmPAL2 to be valuable biocatalysts for the synthesis of trans -cinnamic acid and p -coumaric acid. In addition, MD simulations revealed that the underlying mechanisms of the increased catalytic efficiency of both mutant PT10 and PA1 are attributed to the substrate remaining stable within the pocket and closer to the catalytically active site. This also provides a new perspective on engineered PAL.
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Manjia Lu,Shirong Sun,Xiaoya Li,Qiyu Liu,Wenli Zhang,Xueqing Qiu
Two distinctive aromatic units, p − coumarate and ferulate, exist in corncob lignin, which have the potential to yield p − coumaric acid ( p CA) and ferulic acid (FA). Although p CA and FA are primarily extracted from corncob lignin utilizing strong acids and bases, extremely acidic or alkaline conditions result in the disruption of the aromatic unit structure of the residual lignin. Herein, lactic acid coupled with choline chloride was utilized as acidic deep eutectic solvent (DES), while K 2 CO 3 with glycerin was used as alkaline DES, thereby facilitating the extraction of p CA, FA and lignin from corncob in a mild environment. Furthermore, the differences in monophenol yields from pyrolysis of lignin were investigated. The alkaline DES exhibited a stronger extraction capacity for these acids. The yields of p CA and FA were 15.47 mg/g and 7.44 mg/g (based on the weight of corncob). Contrastively, the subsequent pyrolysis process yielded a higher amount of monophenol from the lignin extracted using acidic DES, with notably greater quantities of low methoxy phenolic monomers. This renders it a preferred option for subsequent processing into high−calorific biofuels. This work presents a straightforward and efficient strategy for the deconstruction of the lignin from corncob to enhance the utilization value of agricultural wastes.
研究领域:
生物质化学
·
能源科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Meng Zhang,Yuqi Zheng,Yang Jin,Dong Wang,Guohui Wang,Xin Zhang,Yanxin Li,Shaoxiang Lee
Developing novel bilayer food packing film having the ability to prevent bacterial infections and capable of inhibiting oxidation is utmost important, since bacterial infections and oxidation can cause food spoilage. Ag-Metal−organic framework loaded p-coumaric acid modified chitosan (P-CS/Ag@MOF) or chitosan nanoparticles (P-CSNPs/Ag@MOF) and polyvinyl alcohol/starch (PVA/ST) were used as the upper film and lower layer film to successfully prepare a bilayer composite film. The microscopic morphology, water resistance, oil resistance, oxidation resistance, optical properties, cytotoxicity and antibacterial properties of the composite films were compared. The results showed that the surface of P-CS/Ag@MOF bilayer was relatively smooth and its tensile strength (TS) was higher (27.67 MPa). Among them, P-CS/Ag@MOF bilayer films had better oil resistance and oxidation resistance activity. In addition, the P-CS/Ag@MOF bilayer film had good UV-blocking properties and transparency. P-CSNPs/Ag@MOF bilayer film had higher antibacterial activity and cytotoxicity.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 14
·5309
·
IF 4.6
Shi-Hui Lu,Xiao-Na Liang,Xiao-Jin Nong,Ran Chen,Xiu-Xia Li
Trans-p-hydroxycinnamic acid and its esters in the leaves ofLigustrum robustummight be a new resource to prevent diabetes and its complications. In the present study, a new HPLC-UV method using hydrolyzation with sodium hydroxide for quantitation oftrans-p-hydroxycinnamic acid and totaltrans-p-hydroxycinnamic acid esters in the leaves ofL. robustumwas developed, since it was difficult and troublesome to analyze no less than 34trans-p-hydroxycinnamic acid esters by usual HPLC. The extract ofL. robustumwas hydrolyzed with sodium hydroxide at 80 °C for 2 h, and then, hydrochloride was added. HPLC analysis was performed in reverse phase mode using a C-18 column, eluting with methanol-0.1% acetic acid aqueous solution (40:60,v/v) in isocratic mode at a flow rate of 1.0 mL·min−1and detecting at 310 nm. The linear range fortrans-p-hydroxycinnamic acid was 11.0–352.0 μg·mL−1(r2= 1.000). The limit of detection and limit of quantification were 2.00 and 6.07 μg·mL−1, respectively. The relative standard deviations of intra-day and inter-day variabilities fortrans-p-hydroxycinnamic acid were less than 2%. The percentage recovery oftrans-p-hydroxycinnamic acid was 103.3% ± 1.1%. It is the first HPLC method using hydrolyzation for quantification of many carboxylic esters. Finally, the method was used successfully to determinetrans-p-hydroxycinnamic acid and totaltrans-p-hydroxycinnamic acid esters in various extracts of the leaves ofL. robustum. The 60–70% ethanol extracts ofL. robustumshowed the highest contents of freetrans-p-hydroxycinnamic acid (3.96–3.99 mg·g−1), and the 50–80% ethanol extracts ofL. robustumdisplayed the highest contents of totaltrans-p-hydroxycinnamic acid esters (202.6–210.6 mg·g−1). The method can be applied also to the quality control of the products ofL. robustum.Keywords:trans-p-hydroxycinnamic acid;esters;Ligustrum robustum;HPLC-UV;hydrolyzation;sodium hydroxide;quantification
研究领域:
植物化学
·
分析化学
·
药用植物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2025
·
IF 6
Chunhong Zhang,Zhijie Qi,Zizheng Ma,Yuning Gong,Han Feng,Junbo Zang,Weifeng Du,Lei Shang
Commercial epoxy resins are significantly limited in their applications in structural materials, packaging materials, and other fields due to inherent drawbacks such as the non-renewability of synthetic raw materials, high brittleness, and flammability. During the modification process, challenges related to the compatibility of additives and the “see-saw” issue between mechanical performance and flame retardancy persistently arise. This study presents the first synthesis of sustainable flame-retardant epoxy resins (p-CAIE) using biomass-derived p-coumaric acid (p-CA) and isophorone diisocyanate (IPDI) as raw materials. p-CAIE is utilized as a modifier for commercial epoxy resins, resulting in thermosetting materials (p-CAIE-E51/DDM) with excellent thermal stability, superior mechanical properties, and enhanced flame retardancy. The compatibility between the components is significantly improved, and the introduction of extensive hydrogen bonding within the crosslinked network leads to remarkable enhancements in performance. Specifically, the tensile strength and elongation at break of the 15 % p-CAIE-E51/DDM cured resin system increased by 119 % and 360 %, respectively, while the impact strength improved by 151 % compared to pure E51/DDM. The limiting oxygen index (LOI) reached 29.5 %, and the UL-94 test achieved a V-0 rating. Additionally, the peak heat release rate (PHRR), total heat release (THR), total smoke production (TSP), and carbon monoxide production (COP) were reduced by 54.2 %, 55.5 %, 53.6 %, and 40.5 %, respectively, effectively suppressing thermal and smoke release. The material also exhibited high transparency within the visible light range (400 nm-760 nm). Thus, this research provides an environmentally friendly strategy for the multifunctional manufacturing of bio-based epoxy resins, offering a promising solution for simultaneously enhancing the flame retardancy, toughness, and transparency of thermosetting epoxy materials.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 11
·2269
·
IF 4.926
Yan-Lu Wang,Wei-Kang Wang,Qi-Chao Wu,Fan Zhang,Wen-Juan Li,Sheng-Li Li,Wei Wang,Zhi-Jun Cao,Hong-Jian Yang
In plant cell wall, ferulic acid (FA) andp-coumaric acid (pCA) are commonly linked with arabinoxylans and lignin through ester and ether bonds. These linkages were deemed to hinder the access of rumen microbes to cell wall polysaccharides. The attachment of rumen microbes to plant cell wall was believed to have profound effects on the rate and the extent of forage digestion in rumen. The objective of this study was to evaluate the effect of bound phenolic acid content and their composition in corn silages on the nutrient degradability, and the composition of the attached bacteria. Following an in situ rumen degradation method, eight representative corn silages with different FA andpCA contents were placed into nylon bags and incubated in the rumens of three matured lactating Holstein cows for 0, 6, 12, 24, 36, 48, and 72 h, respectively. Corn silage digestibility was assessed by in situ degradation methods. As a result, the effective degradability of dry matter, neutral detergent fibre, and acid detergent fibre were negatively related to the ether-linked FA andpCA, and their ratio in corn silages, suggesting that not only the content and but also the composition of phenolic acids significantly affected the degradation characteristics of corn silages. After 24 h rumen fermentation, Firmicutes, Actinobacteria, and Bacteroidota were observed as the dominant phyla in the bacterial communities attached to the corn silages. After 72 h rumen fermentation, the rumen degradation of ester-linked FA was much greater than that of ester-linkedpCA. The correlation analysis noted thatErysipelotrichaceae_UCG-002,Olsenella,Ruminococcus_gauvreauii_group,Acetitomaculum, andBifidobacteriumwere negatively related to the initial ether-linked FA content whilePrevotellawas positively related to the ether-linked FA content and the ratio ofpCA to FA. In summary, the present results suggested that the content of ether-linked phenolic acids in plant cell walls exhibited a more profound effect on the pattern of microbial colonization than the fibre content.Keywords:corn silage;rumen-attached bacteria;phenolic acids;in situ degradation
研究领域:
动物营养学
·
微生物学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）