Forests
·
2024
·Vol. 15
·期 7
·1193
·
IF 2.4
Yingni Huang,Qianlin Li,Chunmei Bai,Wendi Zhao,Diego Ismael Rodríguez-Hernández,Xiali Guo
Recent studies on the intra-annual dynamics of trees were mainly focused on mature trees in natural forests; however, less is known about how topography (e.g., slope’s position and aspect) shape the intra-annual xylem formation dynamics of young trees in plantation forests. We monitored intra-annual xylem formation dynamics of 6-year-old Chinese fir (Cunninghamia lanceolata(Lamb.)) trees across two different aspects (northeast and southwest) and three different positions (upper, middle, and lower) of the slope in a planted forest in subtropical China. We found that the intra-annual xylem formation of trees on the northeast slope aspect (176.98 ± 34.52 cells) was significantly higher than that on the southwest slope aspect (140.19 ± 36.07 cells) due to the higher growth rate (0.67 ± 0.11 cells/day vs. 0.53 ± 0.10 cells/day). In the middle slope position, xylem formation (187.89 ± 19.81 cells) was also significantly higher than that of the upper (147.35 ± 29.08 cells) and lower slope positions (140.51 ± 48.36 cells), resulting from higher growth rate rather than longer growing season length. Our study demonstrated that intra-annual xylem formation dynamics of Chinese fir were altered by different topographic features and therefore encourage the implementation of management strategies that consider different slope aspects and positions to maximize forest productivity.
研究领域:
林学
·
生态学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Ni Wei,Chundong Yang,Yan Su,Chenhuan Lai,Qiang Yong
To investigate the disparate effects of carbocation scavengers on promoting the enzymatic hydrolysis of softwood and hardwood, five carbocation scavengers were supplemented during acid pretreatment of masson pine and poplar in this study, including 2-naphthol (2 N), 2-naphthol-7-sulfonate (NS), p -hydroxybenzoic acid (PB), vanillic acid (VA) and syringic acid (SA). The results showed that naphthols (2 N and NS) with only one nucleophilic site enhanced delignification and enzymatic hydrolysis more significantly than the monophenols with several reactive sites. Adding 2 N and NS increased the hydrolysis yield of acid-pretreated masson pine (AM) from 14.87% to 20.58% and 35.52%, respectively. Correspondingly, the hydrolysis yield of acid-pretreated poplar (AP) improved from 56.54% to 80.47% and 86.9%, respectively. Moreover, as pretreatment severity increased, the enzymatic digestibility of AM and AP first increased and then decreased. However, the naphthols modification retarded this decline, especially for the enzymatic hydrolysis of pretreated poplar. The structure of carbocation scavengers and the lignin unit composition jointly determined the improvement on enzymatic hydrolysis of woody biomass by carbocation scavengers. Furthermore, the naphthols promoted the enzymatic hydrolysis mainly by alleviating enzyme non-productive binding.
研究领域:
生物化学
·
生物质能
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Xiaoxue Zhao,Xin Xu,Zhe Ling,Caoxing Huang,Chenhuan Lai,Qiang Yong
Developing green pretreatment technologies that prioritize the selective separation of lignin is of significant importance for the industrialization of biorefining. This work employed H 2 SO 4 -catalyzed γ-valerolactone (GVL) pretreatment on masson pine sawdust (GAM) to investigate process conditions and the enzymatic hydrolysis performance of masson pine. The results indicated that the lignin removal rate was 87.2% under the optimal pretreatment conditions (150ºC, 75 mM H 2 SO 4 ). The synergistic effect of the H 2 SO 4 catalyst and GVL pretreatment resulted in an enzymatic hydrolysis yield for GAM (76.8%) nearly six times higher than that of other non-synergistic systems (5.7%∼13.0%). Furthermore, the enzymatic hydrolysis yield increased to 97.3% upon the addition of bovine serum albumin . Microscopic visualization was used to analyze the morphological changes of cells and the micro-distribution of lignin within GAM cell walls after pretreatment. Additionally, structural changes in the molecular weight , functional groups, and linkage contents of GAM lignin were analyzed. This research provides valuable insights for masson pine in producing fermentable monosaccharides and acquiring lignin with enhanced processing properties.
研究领域:
生物化学
·
生物质能
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
Forests
·
2024
·Vol. 15
·期 2
·244
·
IF 2.4
Quanmin Wen,Ruixu Chen,Tian Xu,Dejun Hao
Conifers have evolved sophisticated terpenoid defenses for protection against herbivores and pathogens.Pinus massonianaLamb. is the most widely distributed pioneer afforestation and resin tree species in China, but is seriously harmed by pine wilt disease.Monochamus alternatusis the main vector of pine wilt disease in China. Monoterpenes, sesquiterpenes and diterpenes, the main secondary defensive compounds ofP. massoniana, are catalyzed by different terpene synthases (TPSs), which participate in the important defense pathways against external biotic and abiotic stresses. Here, we aimed to identify the terpene synthases (TPSs) inP. massoniana,responding to the feeding ofM. alternatus, and to characterize the functions and products of the mono-TPSs. We identified six differentially expressed TPS genes in theP. massonianafed upon byM. alternatus, including four mono-TPS and two sesqui-TPS genes. The functions of the four mono-TPSs were verified by analysis of the main product and by-products of these mono-TPSs. (+)-α-Pinene, (−)-α-pinene, and limonene were the major products of TPS (+)-α-pinene, TPS (−)-α-pinene, and TPS limonene, respectively, but TPS (−)-β-pinene only catalyzed a trace amount of (−)-β-pinene in the products. Our findings shed light on the potential relationships between the structure of terpene synthases and their corresponding products.
研究领域:
植物学
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分子生物学
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生态学
✓ 已验证
产品关联
DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2023
·
IF 6.5
Zijing Zhou,Zhengchun Li,Fuhua Fan,Huijuan Qin,Guijie Ding
Gibberellins (GAs) play a crucial role in regulating secondary growth in angiosperms, but their effects on the secondary growth of gymnosperms are rarely reported. In this study, we administered exogenous GA 3 to two-year-old P. massoniana seedlings, and examined its effects on anatomical structure, physiological and biochemical changes, and gene expression in stems. The results showed that exogenous GA 3 could enhance xylem development in P. massoniana by promoting cell division. The content of endogenous hormone (including auxins, brassinosteroids, and gibberellins) were changed and the genes related to phytohormone biosynthesis and signaling pathway, such as GID1 , DELLA , TIR1 , ARF , SAUR , CPD , BR6ox1 , and CYCD3 , were differentially expressed under GA 3 treatment. Furthermore, GA 3 and BR (brassinosteroid) might act synergistically in promoting secondary growth in P. massoniana . Additionally, lignin content was significantly increased after GA 3 treatment accompanied by the express of lignin biosynthesis related genes. PmCAD (TRINITY_DN142116_c0_g1), a crucial gene involved in the lignin biosynthesis, was cloned and overexpressed in Nicotiana benthamiana , significantly promoting the xylem development and enhancing stem lignification. It was regarded as a key candidate gene for improving stem growth of P. massoniana . The findings of this study have demonstrated the impact of GA 3 treatment on secondary growth of stems in P. massoniana , providing a foundation for understanding the molecular regulatory mechanism of stem secondary growth in Pinaceae seedlings and offering theoretical guidance for cultivating new germplasm with enhanced growth and yield.
研究领域:
植物生理学
·
分子生物学
·
林业
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2023
·
IF 1.3
Xia Chenjie,Zhu Hongliu,Hu Changhui,Liu Jie,Hua Haibing,Yang Jianqiu,Chen Zhipeng,Gao Yihong
Pinus massoniana needles, a traditional herb, were applied to prevent hair loss in China. Studies available mainly focused on pine needle flavonoids with various biological activities. However, there has been no pharmacokinetics study of the flavonoids from Pinus needles extract. A selective and sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously quantify taxifolin, quercetin and catechin in rat plasma. To separate the three constituents, an Agilent Extend-C18 column (2.1 mm × 100 mm, 1.8 μm) was used with a mobile phrase of (A) 0.1% formic acid and (B) acetonitrile. The analytes were measured by multiple reaction monitoring in the negative ionization mode. There was good linearity in the established UHPLC-MS/MS method, with a coefficient of determination (r2) of >0.99. The accuracy, intra-day and inter-day precision and recovery were all satisfactory and these 3 compounds were stable under the tested conditions. The validated method in this study was successfully applied to pharmacokinetic study in healthy rats after oral and transdermal administration of Pinus needles extract. The results could provide further research foundation for pine needle extract as external preparations.
研究领域:
中药学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shutong Fan,Xun Gao,Xi Yang,Xianjun Li
To be suitable for certain construction and furniture applications, wood must be treated with a flame retardant and impregnating flame retardants into the cellulose lumens of wood is an effective flame retardant method. Phytic acid , the main storage form of phosphorus in various plant tissues, is an inexpensive, and non-toxic biomaterial that shows potential applications as an environmentally friendly bio-based flame retardant. In this study, phytic acid and zinc phytate were used to impregnate delignified wood under vacuum and pressure, which greatly enhanced the flame retardancy and smoke suppression properties of Chinese fir , while still maintaining its original texture. Phytic acid and zinc phytate were hydrogen-bonded to cellulose in wood. Phytic acid and zinc phytate were hydrogen-bonded to cellulose in wood. The results showed that the total heat release (THR) of Chinese fir treated with zinc phytate decreased from 55.66 MJ/m 2 to 5.90 MJ/m 2 , and a compact carbonized protective layer was quickly formed on the surface of Chinese fir after ignition. Thermogravimetric analysis (TGA) showed that the char yield of Chinese fir treated by the flame retardant was 177.6 % higher than that of untreated wood. This study provides an efficient, sustainable, and economical method to prepare Chinese fir with excellent flame retardancy and thermal insulation performance.
研究领域:
材料科学
·
消防安全工程
·
可持续化学
·
木材科学与技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhichen Wang,Ruili Geng,Yanai Chen,Jianglei Qin,Shuai Guo
The incidence and mortality rates of lung cancer have remained high for several decades, necessitating the discovery of new drugs and the development of effective treatment strategies. This study identified matairesinoside (MTS) as a potent inhibitor of TMEM16A, a novel drug target for lung cancer. Molecular simulation combined with site-directed mutagenesis experiments confirmed the key binding sites of MTS and TMEM16A. Cell experiments demonstrated that MTS significantly inhibited the growth, migration, and invasion of lung cancer cells, while inducing apoptosis. Gene knockdown and overexpression studies further revealed that TMEM16A is the target for MTS in regulating lung cancer cell growth. Western blot analysis elucidated the signaling transduction network involved in MTS-mediated regulation of lung cancer. Building upon these findings, a biodegradable self-healing functional hydrogel was developed to load MTS, aiming to enhance therapeutic efficacy and minimize side effects in vivo . Animal experiments demonstrated that the hydrogel/MTS formulation exhibited satisfactory inhibitory effects on lung cancer and mitigated the side effects associated with direct MTS injection. This study identified MTS as a potential candidate for anti-lung cancer therapy with well-defined pharmacological mechanisms. Moreover, the targeted drug delivery system utilizing the hydrogel/MTS platform offers a promising approach for lung cancer treatment.
研究领域:
肿瘤学
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药理学
·
分子生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Fei Li,Yang Liu,Jia Jia,Hongbo Yu
Lignocellulosic biorefineries depended on effective pretreatment strategies to improve the conversion efficiency of the enzymatic hydrolysis. Here, this study coupled brown rot fungi and deep eutectic solvent (DES) to pretreat Pinus massoniana . The results showed that compared to fungal pretreatment and DES pretreatment alone, the combined ChCl-Lac/fungal pretreatments could effectively improve enzymatic saccharification of Pinus massoniana . The highest content of releasing reducing sugar reached 510.3 mg/g substrate. Environmental scanning electron micrograph (ESEM) showed that the surface structure of Pinus massoniana was almost completely torn and loose and FT-IR spectra and component analysis revealed that most of hemicellulose and lignin were selected removed and cellulose was enriched after ChCl-Lac/fungal pretreatments, which could account for the enhanced hydrolysis efficiency. The combination of biological pretreatment with DES pretreatment could be a mild and promising pretreatment approach for enzymatic saccharification of lignocellulose and had an extensive application prospect in the field of biorefinery.
研究领域:
生物技术
·
生物化学工程
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2022
·
IF 8.634
Luan Yang,Tianran Zheng,Chen Huang,Jianfeng Yao
Bioconversion of renewable biomass into various useful chemicals is considered as the main way to achieve low-carbon development. Here, efficient conversion of masson pine was realized through biological refining as follows: using deep eutectic solvent (choline chloride/lactic acid) pretreatment to remove lignin and degrade hemicellulose ; adding UiO-66-NH 2 to boost enzymatic hydrolysis yield; and reusing extracted lignin to prepare a functional material. The effects of pretreatment conditions on enzymatic hydrolysis and the purity of the regenerated lignin were studied. Under the optimal condition (130 °C, 1:10, 4 h), lignin removal rate, lignin recovery, and the purity of the recovered lignin were 81.2%, 72.3%, and 92.5%, respectively. The hydrolysis yield of cellulose at 5 FPU/g-glucan enzyme dosage was increased from 42.1% to 52.8% after using UiO-66-NH 2 . The regenerated lignin was further utilized to synthesize an ultraviolet shielding film. In short, this work can achieve efficient utilization of all components of masson pine.
研究领域:
生物技术
·
化学工程
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Food & Function
·
2020
·Vol. 12
·期 1
·252-266
·
IF 4.171
Xiangyun Niu,Hongqi Shang,Siyan Chen,Ruichang Chen,Jin Huang,Yongqiang Miao,Wenping Cui,Huan Wang,Zhou Sha,Duo Peng,Ruiliang Zhu
The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine. On the contrary, disordered dynamic processes lead to chronic inflammation and disease pathology. Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation. However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown. In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice. The result showed that PPPS regulated the composition of gut microbiota in mice and increased the proportion of probiotics. Subsequently, we established immunosuppressive mice using cyclophosphamide (CTX) and found that PPPS regulated the immunosuppressive state of lymphocytes in Peyer's patches (PPs). Moreover, PPPS also regulated systemic immunity by acting on intestinal PPs. PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells. In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice. Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.
研究领域:
免疫学
·
微生物学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Cheng Cai,Xueqing Qiu,Xuliang Lin,Hongming Lou,Yuxia Pang,Dongjie Yang,Siwei Chen,Kaifan Cai
Two pretreatment methods to overcome recalcitrance of lignocelluloses, sulfite pretreatment (SPORL) and dilute acid (DA), were conducted to pretreat softwood masson pine and hardwood eucalyptus for enzymatic hydrolysis. In the presence of corresponding pre-hydrolysates, adding moderate cetyltrimethylammonium bromide (CTAB) could enhance the enzymatic hydrolysis of the SPORL-pretreated substrates, but had no enhancement for the DA-pretreated substrates. The results showed that sodium lignosulfonate (SL) in pre-hydrolysates and CTAB together had a strong enhancement on the enzymatic hydrolysis of lignocelluloses. The compound of commercial lignosulfonate SXSL and CTAB (SXSL-CTAB) could enhance the substrate enzymatic digestibility (SED) of SPORL-pretreated masson pine from 27.1% to 71.0%, and that of DA-pretreated eucalyptus from 37.6% to 67.9%. The mechanism that CTAB increased the adsorption of SL on lignin to form more effective steric hindrance and reduced the non-productive adsorption of cellulase on lignin by neutralizing the negative charge of SL was proposed.
研究领域:
生物化学
·
生物技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）