Haibin Liu,Tingting Cao,Jing Wang,Yi Yuan,Haijie Li,Kuo He,Hongshuo Chen,Lei Wang
Meat adulteration is a growing concern for consumers and researchers, necessitating the development of a simple and robust method for identifying animal sources in meat products. In this study, a double-tailed recombinase polymerase amplification (RPA) integrated with surface-enhanced Raman scattering (SERS) based two-color lateral flow nucleic acid hybridization strip (LFNAHS) was established for simultaneous detection of pork and horse meat. Magenta and cyan gold nanoparticles functionalized with specific probes and Raman dyestuff were used to color the LFNAHS based on RPA amplification products. The assay successfully detected colored test lines for horse and pork adulteration, with quantitative analysis achieved through Raman intensity measurements. The method showed a high specificity with no cross-reactivity and a detection limit of 0.01 % (wt%). Authenticity testing of 40 donkey products yielded results consistent with PCR-agarose gel electrophoresis. This assay can be extended to identify other meat sources by replacing the specific primer sets. This versatile method can be adapted for identifying other meat sources, making it a valuable tool for accurate, sensitive, and quantitative meat authentication in resource-limited settings.
Yaxin Yu,Fei Xia,Qi Cao,Hanyuan Zhang,Wanting Shi,Peng Zhang,Wenxiu Sun
The adoption of innovative active food packaging has become increasingly crucial for suppressing microbial proliferation and prolonging the shelf life of food items. In this research, we detail the development of novel photodynamic antimicrobial nanofiber films via the electrospinning method, employing polyvinyl alcohol (PVA) as the base material and erythrosine B (EB) as the photosensitizing agent. We examined the chemical composition, photophysical characteristics, and antibacterial properties of these films. FTIR spectroscopy validated the integration of EB within the nanofiber structures. Furthermore, EB enhanced the films' resistance to water, lowered water vapor permeability, and displayed moderate mechanical strength. The nanofiber films exhibited a remarkable capability for generating reactive oxygen species and showed significant antibacterial effectiveness against E. coli , S. aureus , P. putida , and P. aeruginosa , maintaining an overall migration limit of EB within the acceptable threshold of 10 mg/dm 2 . We utilized PVA/EB-G films along with tray packaging to preserve chilled horsemeat, assessing physicochemical alterations and microbial populations. The storage life of chilled horsemeat was prolonged by 6 days in comparison to controls that did not include PVA/EB-G films. The results of this investigation propose a novel strategy for harnessing photodynamic mechanisms in the preservation of chilled meat.
Jianmei Li,Yuting Yin,Haiyu Li,Zongyao Zhao,Mengqin Xiang,Yong Tang,Qi Sun
The escalating economic and ethical concerns associated with camel meat adulteration using cheaper horsemeat necessitate the development of rapid, on-site detection technologies that overcome the limitations of conventional laboratory-based methods. In this study, a portable electrochemical DNA biosensor was fabricated by integrating a polydopamine (PDA)-modified screen-printed carbon electrode (SPCE) with a nucleic acid-functionalized UIO-66(Zr) metal-organic framework (MOF). The PDA coating facilitated stable, covalent immobilization of DNA capture probes through Schiff base reactions, while the UIO-66(Zr) MOF functioned as a high-capacity signal amplifier owing to its large surface area and intrinsic redox activity. This synergistic architecture enabled ultrasensitive detection, achieving a limit of 3.57 pmol/L for horsemeat DNA and 0.5 g/kg adulteration in camel meat blends—significantly surpassing the performance of existing detection methods. The biosensor exhibited excellent specificity by distinguishing single-base mismatches, along with high reproducibility (RSD = 4.12%), operational stability over 15 days, and accurate recovery rates ranging from 91.7% to 112.4% in spiked samples. Its disposable electrode format, cost-effectiveness, and compatibility with portable potentiostats underscore its strong potential for on-site meat authentication. Overall, this work provides a practical and scalable solution to combat meat fraud in resource-limited settings and establishes a versatile MOF-electrode nanoassembly platform for advanced food safety monitoring.
危险品化学品经营许可证（带存储）