CHEMOSPHERE
·
2019
·
IF 5.108
Tianlang Zhang,Junlang Qiu,Guosheng Chen,Jianqiao Xu,Fang Zhu,Gangfeng Ouyang
In recent years, pharmaceuticals have received increasing attentions because of their potential risks to the environment, but researches focusing on their impacts on defense system of living plants are still lacking. As an important class of phytohormones, jasmonates play crucial roles in plant defense system against environmental stress. In order to investigate the effect of pharmaceuticals uptake on endogenous jasmonates, an in vivo solid phase microextraction (SPME) method was established to simultaneously detect and monitor both pharmaceuticals and jasmonates in living plants. The proposed method exhibited wide linear ranges, high sensitivity (limits of detection ranging 0.0043–0.035 ng g −1 for pharmaceuticals and 0.091–0.22 ng g −1 for jasmonates, respectively), and satisfactory reproducibility (relative standard deviation of intrafiber ranging 4.2%–8.6% and interfiber ranging 5.2%–8.2%, respectively). Subsequently, this method was successfully applied to track the concentrations of each pharmaceutical and corresponding jasmonates in living Malabar spinach plants ( Basella alba. L ) exposed to three common pharmaceuticals (i.e. gemfibrozil, mefenamic acid and tolfenamic acid) over 15 days. In result, all pharmaceuticals appeared to trigger intensive biosynthesis of jasmonic acid (JA) (3.1–9.4 times of control) while reduced the concentration of methyl jasmonate (MeJA) (18.3%–38.1% of control). We inferred that uptake of pharmaceuticals acted as an abiotic stress and stimulated the plant defense response because of the variation of jasmonates. To the best of our knowledge, this is the first study applying SPME to detect and track both pharmaceuticals and phytohormones in living plants, which not only provided a glimpse to the adverse effect of pharmaceuticals on plants as well as the regulation of endogenous jasmonates, but also set a promising template for future in vivo analysis of xenobiotics and plant endogenous substances.
研究领域:
植物学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
GENOMICS
·
2024
·
IF 3.4
Jiahui Li,Yuyang Chen,Shuang Zhang,Yuehan Zhao,Demeng Gao,Jiaying Xing,YuYan Cao,Guangyu Xu
This study investigated the preventive and protective effects of Portulaca oleracea polysaccharides (PP) on Acute liver injury (ALI) in mice and its regulatory effects on intestinal microorganisms, and explored the underlying protective mechanisms. Initially, PP was administered, and then CCl4 was used to induce the mouse ALI model. Serum and liver markers were measured by ELISA. The fecal microbiota was analyzed by 16S rRNA sequencing. The results showed that PP significantly decreased the expression levels of ALT and AST in the serum of mice. The expression levels of MDA, TNF-α, and IL-6 in liver tissue were found to be reduced, while the levels of GSH and SOD increased. At the same time, PP increased the number of Bacteroides , reduced the number of Proteobacteria , activated the GAG degradation pathway, protected the integrity of the intestinal barrier, inhibited oxidative stress and reduced inflammation, thereby assisting the prevention and protection of ALI.
研究领域:
药理学
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肝脏病学
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微生物组学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Tianlang Zhang,Nan Li,Guosheng Chen,Jianqiao Xu,Gangfeng Ouyang,Fang Zhu
Due to their wide applications and extensive discharges, pharmaceuticals have recently become a potential risk to aquatic and terrestrial organisms. The uptake of pharmaceuticals have been shown to stimulate plant defense systems and induce phytotoxic effects. Signaling molecules such as plant hormones play crucial roles in plant stress and defense responses, but the relationship between these molecules and pharmaceutical uptake has rarely been investigated. In this study, two common pharmaceuticals, carbamazepine and ibuprofen, and three stress-related plant hormones, jasmonic acid, salicylic acid , and abscisic acid , were simultaneously tracked in the roots and stems of Malabar spinach ( Basella alba L.) via an in vivo solid phase microextraction (SPME) method. We also monitored stress-related physiological markers and enzymatic activities to demonstrate plant hormone modulation. The results indicate that pharmaceutical uptake, subsequent stress symptoms, and the defense response were all significantly correlated with the upregulation of plant hormones. Moreover, the plant hormones in the exposure group failed to recover to normal levels, indicating that plants containing pharmaceutical residues might be subject to potential risks.
研究领域:
植物学
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环境科学
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药理学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Xiankun Liu,Shiqiang Wen,Weichen You,Xiaofei Wang,Qing X. Li,Qiang Bian,Pei Lv,Rimao Hua
An efficient synthesis method will allow a large number of tetramic acid analogues to be synthesized for property and potency optimization. In this study, a facile and efficient method was described for the synthesis of 3-acyltetramic acids. The synthesis was accomplished mainly via (1) mild intramolecular cyclization and (2) the formation of β-ketoamides between nucleophiles and acyl Meldrum’s acids. 3-Acyltetramic acid exhibited phytotoxicity against Echinochloa crusgalli and Portulaca oleracea. At a dosage of 750 g ha–1, 6k and 6a showed high herbicidal activity against E. crusgalli, Digitaria sanguinalis and P. oleracea, Amaranthus retroflexus, respectively. 6k inhibited the synthesis of endogenous abscisic acid, thus seedling germination and plant growth. The incorporation of various acyl Meldrum’s acids and amino acid esters was applicable to the parallel synthesis of 3-acyltetramic acids. The mode of action and herbicidal activity indicate that 3-tetramic acid had good herbicidal performance and was a promising herbicide candidate. This study will provide a reference for novel herbicide development.
研究领域:
化学
·
农业科学
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植物生理学
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DOI
PubMed
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 7
·1400
·
IF 7
Ke Ning,Yameng Duan,Weiwei Tong,Yue Chen,Qinghui Zhang,Qiuhong Xie,Hongyu Xiang
Purslane, a common wild vegetable, contains active substances with various biological functions. However, its effects have been under-investigated in ulcerative colitis (UC). Therefore, this study investigated the therapeutic effects of purslane macromolecular (POEM) and small molecular extracts (POES) on dextran sulfate sodium (DSS)-induced UC in mice. Membrane separation was used to obtain extracts of different molecular weights, and their compositional differences were compared using liquid chromatography–mass spectrometry (LC/MS). POEM contained more proteins and polysaccharides, whereas POES contained more organic acids and alkaloids. These differences in composition were directly responsible for the different degrees of remission of the alleviated UC in model mice. POEM may alleviate UC by regulating the antioxidant capacity and the gut microbiota, whereas the major alleviatory effect of POES was primarily related to the regulation of antioxidant capacity. The POEM and POES effects identified in this study provide a theoretical basis for the development of purslane as a functional food.Keywords:ulcerative colitis;macromolecular extract;purslane;oxidative stress;small molecular extract;gut microbiota;Portulaca oleraceaL.
研究领域:
药理学
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胃肠病学
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营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Molecular Medicine Reports
·
2025
·Vol. 31
·期 5
·1-14
·
IF 3.5
Xueying Zhang,Lele Meng,Xiaorong Ran,Shuang Li,Changhui Wen
Purslane is a traditional Chinese medicine with a long‑standing history of efficacy in the management of dermatological conditions such as vitiligo. However, the molecular mechanisms underlying its therapeutic effects on vitiligo remain unclear. Therefore, the present study explored these mechanisms using network pharmacology, molecular docking and in vitro experiments. Following the screening process, seven principal active components were identified, namely kaempferol, hesperetin, luteolin, quercetin, arachidonic acid, cycloartenol and β‑sitosterol. In addition, six key targets, namely AKT1, tumor protein p53, peroxisome proliferator‑activated receptor γ (PPARG), estrogen receptor 1, prostaglandin‑endoperoxidase synthase 2 and mitogen‑activated protein kinase 1, and eight pathways in purslane‑based vitiligo treatment were identified. Network pharmacology and molecular docking demonstrated that flavonoids are the key components of purslane likely to mitigate oxidative stress damage in vitiligo. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that the phosphatidylinositol 3‑kinase (PI3K)/AKT, p53 and PPARG signaling pathways are associated with purslane components and vitiligo. In vitro experiments revealed that purslane total flavones (PTF) increased cell viability, decreased ROS levels and increased antioxidant enzyme activities in H2O2‑induced B16F10 cells. In addition, PTF activated the PI3K/AKT signaling pathway in H2O2‑induced B16F10 cells, and the antioxidant effect of PTF was attenuated by a PI3K/AKT inhibitor. In conclusion, the findings of the present study suggest that the flavonoids of purslane contribute, at least in part, to its therapeutic effectiveness in vitiligo by mitigating oxidative stress in melanocytes through the PI3K/AKT signaling pathway.
研究领域:
药理学
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皮肤科
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分子生物学
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传统中药
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Qianqian Han,Lirong Huang,Ying Wang,Shixin Sun,Hao Huang,Fei Li,Fangtian Wang,Ligen Chen,Hongmei Zhang,Yanqing Wang
Novel polysaccharide-platinum conjugated polymers bearing alendronate on Portulaca oleracea polysaccharides (PPS) were designed and synthesized. Their chemical structures and properties were characterized by Fourier transform infrared spectroscopy (FT-IR), 1 H NMR and 31 P NMR spectroscopy, Thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), UV–vis spectrophotometer (UV–vis) and other analysis methods. The results demonstrated that alendronate can be used as the linker of Portulaca oleracea polysaccharides and platinum compounds. Portulaca oleracea polysaccharides-alendronate (PPS-ALN) conjugates exhibited stronger antioxidant ability than PPS. The cytotoxicity assay to cancer cells was tested in vitro, and the Portulaca oleracea polysaccharides-alendronate-platinum (PPS-ALN-Pt) conjugates strongly inhibited the proliferation of cancer cells than PPS and PPS-ALN. The evaluation of complexes affinity toward supercoiled plasmid DNA, displayed a high DNA interaction. Interestingly, the platinum conjugates displayed immunological competence in HeLa cells by cellular immunofluorescence assay. Besides, the cellular platinum accumulation of PPS-ALN-Pt conjugates was higher than that of cisplatin in HeLa cells, implying that the polysaccharide-platinum conjugated polymers might have a synergistically therapeutic application in metal anticancer drug delivery.
研究领域:
药物化学
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癌症研究
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聚合物科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Haijun Chang,Yu Huang,Yuanwei Shi,Yu Hu,Wenbin Zhou
The research aimed to elucidate how purslane extract (PE) inhibited pork myofibrillar proteins (MPs) oxidation and modulated the physicochemical properties in the Fenton oxidation system. This study employed UHPLC-QE-Orbitrap-MS/MS to comprehensively analyze the PE constituents and evaluate their antioxidant properties. Furthermore, the effects of various PE concentrations (0.05, 0.10, 0.15, 0.20, and 0.40 mg/mL) on the structural characteristics of pork MPs in a hydroxyl radical oxidation system were evaluated. The findings indicated that PE was rich in phenolics, with luteolin, kaempferol, genistein, rutin, diosmetin, morin, kaempferol-3-O-rutinoside, isoquercitrin, and isorhamnetin-3-O-neohesperidoside being the nine most prevalent compounds. These compounds contributed to the extract's strong free radical scavenging activity. Experimental results demonstrated that adding 0.20 mg/mL PE significantly reduced the carbonyl content compared with oxidized MPs while increasing total thiol, free thiol, and free amino group levels ( P < 0.05). The protective effect of PE on MP amino acid side chains was superior to that of Butylated hydroxyanisole (BHA). Moreover, PE addition protected the secondary structure of MPs and minimized the α-helix loss caused by oxidation. In addition, PE decreased the intrinsic fluorescence intensity of MPs, enhanced their surface hydrophobicity, and reduced their solubility. SDS-PAGE results indicated that oxidation and PE addition induced MP aggregation, with most of it being reversible. These findings support the potential of PE as a natural antioxidant in meat products and offer a theoretical basis for its functional applications in meat processing.
研究领域:
食品科学
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生物化学
·
食品化学
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抗氧化研究
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蛋白质科学
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天然产物
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氧化应激
·
肉类加工
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Xiaomin Zhao,Xingyu Ren,Haoming Li,Bin Zhang,Xiaojing Fan,Xianchao Feng
Development of effective, safe, and degradable food packaging is essential to meet the demands of consumers and to ensure the continued growth of the food industry. In this study, superabsorbent bioactive aerogels based on cellulose and polyvinyl alcohol combined with the antibacterial bioactive extracts extracted from Portulaca oleracea were fabricated for the preservation of chilled meats. The main physicochemical and mechanical properties of the bioactive aerogels were characterized and evaluated. The aerogels had remarkable water and oil sorption abilities and excellent antibacterial capacities, which could significantly delay spoilage of the chilled meat. Compared with commercialized absorbent pads, the bioactive aerogels effectively delayed color changes and retarded the lipid oxidation of meat, and decreased the growth of bacterium during the storage time. The microbial community analysis showed that the bioactive aerogels treatments have significant impacts on the microbial flora of chilled meat, which can significantly inhibit the growth of Pseudomonas and Escherichia, two primary spoilage microorganisms. These findings suggested that the bioactive aerogels can effectively extend the shelf-life of chilled meat and have great application potential in novel active packaging
研究领域:
食品科学
·
材料科学
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生物技术
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微生物学
·
包装技术
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产品关联
DOI
PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2023
·
IF 2.65
Lei Zhang,Ming-yue Pan,Tao Li,Zhi-min Jin,Zhu Liu,Qiu-yue Liu,Yong Liu,Jia-yuan Ding,Huan Jiang,Xingchen Hou
Quercetin was extracted from Portulaca oleracea L. through biphasic acid hydrolysis to investigate its potential as a suppressor of dipeptidyl peptidase IV (DPP-IV) and its hypoglycemic effect in type 2 diabetic mice. The extraction procedure was optimized utilizing the response surface method (RSM) in a single-factor experimental setting. An extraction efficiency of 0.675% was achieved using the following optimized parameters: 0.064 mol/L vitriol, 1 : 109.155 solid-liquid ratio, and 21.408 min ultrasonication. Overall, findings indicate the effectiveness of quercetin extraction. A mouse model for type 2 diabetes was established to receive oral treatment with various quercetin concentrations for 8 weeks. Fasting blood glucose (FBG) and the DPP-IV activity in the serum were significantly reduced. The weight and insulin levels of the mice in the quercetin group were raised compared to those in the model group ( < 0.01). Quercetin dose-dependently inhibited postprandial blood glucose excursions, as demonstrated by the oral glucose tolerance test. These results confirmed that quercetin has hypoglycemic effects and considerably improves insulin sensitivity via DPP-IV targeting.
研究领域:
药理学
·
生物化学
·
糖尿病研究
·
植物化学
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产品关联
DOI
PubMed
高影响因子
Nutrients
·
2024
·Vol. 16
·期 20
·3549
·
IF 4.8
Yiming Zhang,Shengying Zhu,Yueming Gu,Yanjing Feng,Bo Gao
Background/Objectives: Hyperuricemia (HUA) is a common metabolic disease caused by purine metabolic disorders in the body.Portulaca oleraceaL. (PO) is an edible wild vegetable. Methods: In this study, the regulatory effect of PO on HUA and its potential mechanism were initially elucidated through network pharmacology and experimental validation. Results: The results showed that PO from Sichuan province was superior to the plant collected from other habitats in inhibiting xanthine oxidase (XOD) activity. Berberine and stachydrine were isolated and identified from PO for the first time by UPLC-Q-Exactive Orbitrap MS. The potential molecular targets and related signaling pathways were predicted by network pharmacology and molecular docking techniques. Molecular docking showed that berberine had strong docking activity with XOD, and the results of in vitro experiments verified this prediction. Through experimental analysis of HUA mice, we found that PO can reduce the production of uric acid (UA) in the organism by inhibiting XOD activity. On the other hand, PO can reduce the body ‘s reabsorption of urate and aid in its excretion out of the body by inhibiting the urate transporter proteins (GLUT9, URAT1) and promoting the high expression of urate excretory protein (ABCG2). The results of H/E staining showed that, compared with the positive drug (allopurinol and benzbromarone) group, there was no obvious renal injury in the middle- and high-dose groups of PO extract. Conclusions: In summary, our findings reveal the potential of wild plant PO as a functional food for the treatment of hyperuricemia.
研究领域:
药理学
·
代谢疾病
·
植物化学
·
分子生物学
·
实验验证
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Li Xudong,Cui Mingxuan,Zhu Lijie,Zhang Haoqian,Guan Yunhui,Song Xiangjia,Zhao MengLin,Li Zhiguo,Liao Fei,Shi Liqiang,Zhao Ningning
Portulaca oleracea L. (PL) has significant clinical efficacy in treating ulcerative colitis (UC), which significantly affects patients’ daily life. However, the absorption and metabolism of PL in vivo remain unclear due to the difficulty in detecting organic acids. This study focused on analyzing the pharmacogenetic composition of PL extract in 24 rats ( n = 6) and its targeted network pharmacology. Using UPLC MS with derivatization techniques, plasma, and faeces were collected from healthy and UC rats gavaged with PL extracts. Subsequently, in vitro derivatization of the above biological samples and PL extracts was performed before and after derivatization to qualitatively analyze to ensure biosafety. A total of 61 active constituents and 21 constituents in vivo were detected. After derivatization, the sensitivity for detecting non-acid compositions increased in the negative mode, and the sensitivity for detecting acids also increased in the positive mode. For plasma and intestinal analysis, the detective sensitivity of organic acid was effectively increased for 1–3 times, and healthy rats with PL were detected with higher levels of the active constituents than that of UC rats with PL. Combined with the pharmacological analysis of the targeting network, it might act through the hypoxia-inducible factor-1 (HIF-1) signaling pathway and phosphatidylinositol 3-kinase (PI3K-Akt) signaling pathway. However, individual differences in the samples may limit the generalizability of the results, and further validation with larger sample sizes is needed. This study not only provides a scientific basis for the material basis of the medicinal effects but also provides new ideas and methods for the study of traditional Chinese medicines. Graphical
研究领域:
药理学
·
分析化学
·
中医药学
·
代谢组学
·
炎症性肠病研究
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）